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The map label for this gene is yneF [H]

Identifier: 218548907

GI number: 218548907

Start: 1584406

End: 1585839

Strand: Direct

Name: yneF [H]

Synonym: EFER_1552

Alternate gene names: 218548907

Gene position: 1584406-1585839 (Clockwise)

Preceding gene: 218548906

Following gene: 218548908

Centisome position: 34.53

GC content: 46.93

Gene sequence:

>1434_bases
ATGGATAGCAATTTAATGCAACCTGACCATACCCCCATTTTCCGTTTGTTCAAGGAAGATAATTCATTACGTAACGCTTT
TGTCATATTTGCGTTGACGACACTTTTTTATTTTATCGGTGCCAAACTTCGTCTTGTTCATGAACTCTCGCTCTTCTGGC
CGTTGAACGGCGTGATGGCAGGGGTGTTTGCTCGCTATGTCTGGCTTAACCGACTTCATTATTACGCTGTCAGCTATGTC
GCGATGCTGGTTTATGATGCGATGACAACGCAATGGGGGTGGGTGTCGATCGTTATCAATCTGTCCAATATGGTATTTAT
TATTACCGTTGCACGATTAGTCGCACGCGATAAGCAATTGGGTAAAAATAAATACGAACCAGTCAGTGCATTACGTTTAT
TTAATTATTGCCTGATTGCTGCCTTGTTATGTGCGCTGGTGGGGGCCATTGGTTCTGACAGTATCGATTCACTCTCATTT
GGTCCTTTGTTGGCAGACTGGTTTAGTGAGCAATTTTCTACTGGCGTTCTTATTGTGCCGTGTATGTTGACGCTGGCGCT
CCCGCGAATTGTTCCCCGATTTAAAGCTGAACAACTAATGCCGATAGTGGCGTTGATCATTTCGGTTATAGCCTCGGTGG
TGATTGGCGGAGCAGGGAGTCTGGCGTTTCCATTGCCCGCATTGATCTGGTGTGCTGTGCGCTATACCCCACAGGTGACT
TGTCTGCTGACGTTTGTTACGGGGGCTGTCGAAATTGTGCTGGTGGCAAACTCCGTGATCAATATTGCTGTGGCTTCACC
ATTCTCTATTCCGCAAATGTTTTCCGCTCGTTTGGGGATTGCCACGATGGCCATCTGCCCGATTATGGTTTCCTTTAGTG
TGGCGGCCATCAACTCACTGATGAAACAGGTGGCGCAGCGGGCTGATTTTGATTTTCTGACGCAGGTCTATTCGCGCTCA
GGATTATATGAAGCGCTTAAAAAAACCGAGCTGCGTGGCACGCAACATATAACGGTAATGTTGCTTGATATTGATTACTT
CAAAAGCATTAACGACAGTTTCGGGCATGAGTGTGGCGATAAAGTATTGAGTGTTTTTGCGAAACACGTGCAAAAGATAG
TGGGCAATCAAGGATTGGTGGCGCGCATGGGCGGTGAAGAGTTTGCTGTTGTCGTACCATCGCTTAATGCCCAGGAAGGT
TTGCTGCTGGCAGAGAAAATCCGTAAAGCGGTGGCGCTACATCCTTTCGACTGGCAGCAGAAAACACTCTATCTGACAGT
GAGTATTGGTATTGGTAGTGGGGCAACGCGCTTCGAAACACTGACGGAAGTCTTTGATAAGTTGATGGTGGAAGCAGATG
AGTGTCTGTATCGCTCGAAAAAAGAGGGCCGTAATCGCACCAGCGCTATGAATTATCAACAAAAGGCTGTCTGA

Upstream 100 bases:

>100_bases
CTTGACTTTGAAGTGTATTACGTGTCGCCAATAGCTATGAACATGCTATCCTTTATGAGAATTTACTCATTAGCCTGAGA
TAATTGCCTGTCGCAATTAT

Downstream 100 bases:

>100_bases
CAACTTATGTGATCAACTGCACGTTTTGTCGTTAAGTGCAGGAATTGTCATCATCATTTGATCAAAATAGGGGTTCGTTG
TATTAAAGTTTGCTAACCTG

Product: membrane-associated diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MDSNLMQPDHTPIFRLFKEDNSLRNAFVIFALTTLFYFIGAKLRLVHELSLFWPLNGVMAGVFARYVWLNRLHYYAVSYV
AMLVYDAMTTQWGWVSIVINLSNMVFIITVARLVARDKQLGKNKYEPVSALRLFNYCLIAALLCALVGAIGSDSIDSLSF
GPLLADWFSEQFSTGVLIVPCMLTLALPRIVPRFKAEQLMPIVALIISVIASVVIGGAGSLAFPLPALIWCAVRYTPQVT
CLLTFVTGAVEIVLVANSVINIAVASPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVAQRADFDFLTQVYSRS
GLYEALKKTELRGTQHITVMLLDIDYFKSINDSFGHECGDKVLSVFAKHVQKIVGNQGLVARMGGEEFAVVVPSLNAQEG
LLLAEKIRKAVALHPFDWQQKTLYLTVSIGIGSGATRFETLTEVFDKLMVEADECLYRSKKEGRNRTSAMNYQQKAV

Sequences:

>Translated_477_residues
MDSNLMQPDHTPIFRLFKEDNSLRNAFVIFALTTLFYFIGAKLRLVHELSLFWPLNGVMAGVFARYVWLNRLHYYAVSYV
AMLVYDAMTTQWGWVSIVINLSNMVFIITVARLVARDKQLGKNKYEPVSALRLFNYCLIAALLCALVGAIGSDSIDSLSF
GPLLADWFSEQFSTGVLIVPCMLTLALPRIVPRFKAEQLMPIVALIISVIASVVIGGAGSLAFPLPALIWCAVRYTPQVT
CLLTFVTGAVEIVLVANSVINIAVASPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVAQRADFDFLTQVYSRS
GLYEALKKTELRGTQHITVMLLDIDYFKSINDSFGHECGDKVLSVFAKHVQKIVGNQGLVARMGGEEFAVVVPSLNAQEG
LLLAEKIRKAVALHPFDWQQKTLYLTVSIGIGSGATRFETLTEVFDKLMVEADECLYRSKKEGRNRTSAMNYQQKAV
>Mature_477_residues
MDSNLMQPDHTPIFRLFKEDNSLRNAFVIFALTTLFYFIGAKLRLVHELSLFWPLNGVMAGVFARYVWLNRLHYYAVSYV
AMLVYDAMTTQWGWVSIVINLSNMVFIITVARLVARDKQLGKNKYEPVSALRLFNYCLIAALLCALVGAIGSDSIDSLSF
GPLLADWFSEQFSTGVLIVPCMLTLALPRIVPRFKAEQLMPIVALIISVIASVVIGGAGSLAFPLPALIWCAVRYTPQVT
CLLTFVTGAVEIVLVANSVINIAVASPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVAQRADFDFLTQVYSRS
GLYEALKKTELRGTQHITVMLLDIDYFKSINDSFGHECGDKVLSVFAKHVQKIVGNQGLVARMGGEEFAVVVPSLNAQEG
LLLAEKIRKAVALHPFDWQQKTLYLTVSIGIGSGATRFETLTEVFDKLMVEADECLYRSKKEGRNRTSAMNYQQKAV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=315, Percent_Identity=83.4920634920635, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI1787262, Length=212, Percent_Identity=29.7169811320755, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=36.25, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788381, Length=195, Percent_Identity=27.6923076923077, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=33.5294117647059, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788085, Length=186, Percent_Identity=31.7204301075269, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=28.9156626506024, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787816, Length=188, Percent_Identity=29.7872340425532, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693134, Length=184, Percent_Identity=29.8913043478261, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787056, Length=178, Percent_Identity=33.7078651685393, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 52819; Mature: 52819

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCC
>Mature Secondary Structure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CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]