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The map label for this gene is yiaO [H]

Identifier: 218548882

GI number: 218548882

Start: 1560992

End: 1561966

Strand: Direct

Name: yiaO [H]

Synonym: EFER_1527

Alternate gene names: 218548882

Gene position: 1560992-1561966 (Clockwise)

Preceding gene: 218548881

Following gene: 218548883

Centisome position: 34.02

GC content: 42.46

Gene sequence:

>975_bases
ATGGAAAAGAAAAAACTGTCACTGGCTCTTTGTACGCTGTTTGGCGGGTTATTAAGTTTTTCTGCATATGCGGCTGATGT
CACGTTAAAATTAGGTCACCCTGTTAACGAAGAGCATAGTTGGCATAAAGCGTCGCTTAAATTTGCTGAACTGGTAAAGC
AAAAGACAAATGATGCAGTTGAAGTAAAAATTTATCCTAACGAATCATTAGGTAAAGAGATGGATATTATCAACGCTATC
CAGTTGGGGAGTGTTGATATGACCATCACAGGGGAGTCATTACAAAACTGGGCACCTTCTGCGGCATTACTGGCAGTGCC
TTATATGATTACATCTTCTGAACAAATGGAAAAAATAGTTAATGGGGAAGTCGGTGCGCAAATATCAAAAGATATTGAAG
AACGCGTGAAACTGAAAACTATTGCCTGGTTCGAACGTGGCCCGCGTAATTTGACCTCTAACCGTCCGATCACGAATCCA
CAAGAGTTAAAAGGGCTGACTCTGCGCTTACCTAACGTACCGATGTTCATCAGCGTATGGAAAGATTTAGGCGCGCAGCC
GACACCAATGGCATTCTCAGAAGTGTTTACTGGGCTGCAACAGGGCGTCATTGAGGGGCAAGAAAACCCCTTATCTATGA
TCATGAGCGGTAGTTTCTTTGAAGTGCAGAAATACCTTAACCGGACTGAGCACGTTCGTAGTTGGATCTATCTGACAATG
GGCAGCAAAAAGTTCAACAAGCTGAAGCCTGAATATCAGCAAGCTATTCTGGATGCAGGCAAAGAAGCTCAGGCTTACGA
GCATGAGTTATATCTCCAGGATGAAGAAAAATTAGTTGAAGCTCTGCAAGCGAAAGGGATGACACTGGTTGATGTTCATC
AGAATGAATTTGCTAAAATAGCAAACGAGTCAGTCGAAAAATCAATAAATCCGCAAATTAAGCCTCTTTACGAAAAAGCT
CGCGCCACAAAATAA

Upstream 100 bases:

>100_bases
ATAGAGTCCCAACAAGAAGGTCTTTGCAAAGTATTATTAAAGTTTCCCGAATCCTGACCCTCATTTATAAAAACAACTGT
ACCTACAAGGAACATAGATC

Downstream 100 bases:

>100_bases
CATTAAACGGGGGAGTCGCTTCCCCCTTTTTTGAGCTACAGGGGTTCGGATATGTTTAATAAGCTTTATGGCTATATAGA
AAAGATCTATTTAATTATTG

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MEKKKLSLALCTLFGGLLSFSAYAADVTLKLGHPVNEEHSWHKASLKFAELVKQKTNDAVEVKIYPNESLGKEMDIINAI
QLGSVDMTITGESLQNWAPSAALLAVPYMITSSEQMEKIVNGEVGAQISKDIEERVKLKTIAWFERGPRNLTSNRPITNP
QELKGLTLRLPNVPMFISVWKDLGAQPTPMAFSEVFTGLQQGVIEGQENPLSMIMSGSFFEVQKYLNRTEHVRSWIYLTM
GSKKFNKLKPEYQQAILDAGKEAQAYEHELYLQDEEKLVEALQAKGMTLVDVHQNEFAKIANESVEKSINPQIKPLYEKA
RATK

Sequences:

>Translated_324_residues
MEKKKLSLALCTLFGGLLSFSAYAADVTLKLGHPVNEEHSWHKASLKFAELVKQKTNDAVEVKIYPNESLGKEMDIINAI
QLGSVDMTITGESLQNWAPSAALLAVPYMITSSEQMEKIVNGEVGAQISKDIEERVKLKTIAWFERGPRNLTSNRPITNP
QELKGLTLRLPNVPMFISVWKDLGAQPTPMAFSEVFTGLQQGVIEGQENPLSMIMSGSFFEVQKYLNRTEHVRSWIYLTM
GSKKFNKLKPEYQQAILDAGKEAQAYEHELYLQDEEKLVEALQAKGMTLVDVHQNEFAKIANESVEKSINPQIKPLYEKA
RATK
>Mature_324_residues
MEKKKLSLALCTLFGGLLSFSAYAADVTLKLGHPVNEEHSWHKASLKFAELVKQKTNDAVEVKIYPNESLGKEMDIINAI
QLGSVDMTITGESLQNWAPSAALLAVPYMITSSEQMEKIVNGEVGAQISKDIEERVKLKTIAWFERGPRNLTSNRPITNP
QELKGLTLRLPNVPMFISVWKDLGAQPTPMAFSEVFTGLQQGVIEGQENPLSMIMSGSFFEVQKYLNRTEHVRSWIYLTM
GSKKFNKLKPEYQQAILDAGKEAQAYEHELYLQDEEKLVEALQAKGMTLVDVHQNEFAKIANESVEKSINPQIKPLYEKA
RATK

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=296, Percent_Identity=32.7702702702703, Blast_Score=198, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36388; Mature: 36388

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKKLSLALCTLFGGLLSFSAYAADVTLKLGHPVNEEHSWHKASLKFAELVKQKTNDAV
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEE
EVKIYPNESLGKEMDIINAIQLGSVDMTITGESLQNWAPSAALLAVPYMITSSEQMEKIV
EEEECCCCCCCCHHHHHHHHEECCEEEEEECCHHHCCCCCHHHHHHHHHHCCHHHHHHHH
NGEVGAQISKDIEERVKLKTIAWFERGPRNLTSNRPITNPQELKGLTLRLPNVPMFISVW
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCEEEECCCCCHHHHHH
KDLGAQPTPMAFSEVFTGLQQGVIEGQENPLSMIMSGSFFEVQKYLNRTEHVRSWIYLTM
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEEEEE
GSKKFNKLKPEYQQAILDAGKEAQAYEHELYLQDEEKLVEALQAKGMTLVDVHQNEFAKI
CCHHHHHCCHHHHHHHHHCCCHHHHHHHHEEECCHHHHHHHHHHCCCEEEEECHHHHHHH
ANESVEKSINPQIKPLYEKARATK
HHHHHHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MEKKKLSLALCTLFGGLLSFSAYAADVTLKLGHPVNEEHSWHKASLKFAELVKQKTNDAV
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEE
EVKIYPNESLGKEMDIINAIQLGSVDMTITGESLQNWAPSAALLAVPYMITSSEQMEKIV
EEEECCCCCCCCHHHHHHHHEECCEEEEEECCHHHCCCCCHHHHHHHHHHCCHHHHHHHH
NGEVGAQISKDIEERVKLKTIAWFERGPRNLTSNRPITNPQELKGLTLRLPNVPMFISVW
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCEEEECCCCCHHHHHH
KDLGAQPTPMAFSEVFTGLQQGVIEGQENPLSMIMSGSFFEVQKYLNRTEHVRSWIYLTM
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEEEEE
GSKKFNKLKPEYQQAILDAGKEAQAYEHELYLQDEEKLVEALQAKGMTLVDVHQNEFAKI
CCHHHHHCCHHHHHHHHHCCCHHHHHHHHEEECCHHHHHHHHHHCCCEEEEECHHHHHHH
ANESVEKSINPQIKPLYEKARATK
HHHHHHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]