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The map label for this gene is helD [H]

Identifier: 218548471

GI number: 218548471

Start: 1144593

End: 1146647

Strand: Direct

Name: helD [H]

Synonym: EFER_1099

Alternate gene names: 218548471

Gene position: 1144593-1146647 (Clockwise)

Preceding gene: 218548468

Following gene: 218548474

Centisome position: 24.94

GC content: 49.25

Gene sequence:

>2055_bases
ATGGAACTGAAAGCGACTTCTCTTGGAAAACGTCTGGCGCAGCACCCTTATGATCGGGCGGTCATTCTTAATGCAGGGGT
TAAAGTCTCTGGCGATCGCCATGAGTACCTCATTCCATTTAATCATTTACTGGCAATTAACTGTAAGCGCGGTCTGGTGT
GGGGCGAGCTGGAGTTTGTGTTGCCTGATGAGAAAGTTGTCCGTTTGCATGGTACGGAGTGGGCAGAAACACAACGCTTC
TATCATCACCTTAATGCTCACTGGCAGCAATGGAGCGACGAAATGAGTCAAATTGCCGCCACGGTACTGCAAGAGCAATT
AGCGCTGATTACCCAGCAAACCGGGGAAAATAAATGGCTGACGCGAGATAAAACCCAGGGGATTCAGCAACAAATTCGTC
AGGCATTTTCCGCACTGCCGTTGCCCGTCAGCCGACTGGAAAGCTTTGATAATTGCCGCGATCCATTAAATCAGTGTATG
GCATGGCTGAAAGATATCGAAGGAAGCCGTCGACAACATAATCAGCGATATACCGAGGCGATGATAAAAGAGAATGCTGA
GTTTTTTCAGCAGATAGAGTCCTCCCCGCTGAATCCCGCTCAGACACGGGCAGTTGTCAACGGTGAACACTCTCTGCTGG
TGTTGGCGGGGGCCGGTAGTGGAAAAACATCTGTTCTGGTGGCCCGTGCCGGATGGTTGCTGGCCCGAGGCGAAGCCGCG
GCTGAACAAATTTTATTGCTGGCATTTGGTCGCAAAGCTGCCGAAGAAATGGATGAACGAATTCGTCAGCGTTTGCATAC
TGAGGACATAACGGCACGTACTTTTCACTCCCTTGCTCTTTATATTATTCAGCAGGGCAGTAAAAAATTACCCACGGTCA
GCAAACTGGAAAATGACACTCAGGCCAGGCACGAGCTCTTTATTAAGGAATGGCGTAAACAGTGCAGCGAGAAAAAAGCG
CAGGCGAAAGGCTGGCGGCTATGGCTGGAAGACGAGTTGCAGTGGGATGTGCCGGAAGGGAATTTCTGGGACGATACAAA
ACTGCAACGCCGCCTTGCTTCCCGACTTGATCGTTGGGTCAGTTTAATGCGAATGCACGGCGGCGCACAGGCGGAGATGA
TCGCTGGGGCACCAGAGGATATTCGCGATTTATTCAGTAAACGTATCAAGCTGCTGGCTCCATTGTTAAAAGCCTGGAAA
AGCGCGTTAAAAGCAGAAAATGCAGTGGATTTCTCTGGTCTGATTCATCAGGCGATTGTGATTCTGGAAAAAGGGCGGTT
TATCAGTCCGTGGAAGCACATTCTGGTGGATGAGTTTCAGGATATCTCGCCGCAGAGAGCCGCACTTCTGGCAGCCCTGC
GCAAGCAAAACAGTCAAACCTCACTATTTGCTGTGGGTGACGACTGGCAGGCAATCTACCGTTTCAGTGGTGCACAACTT
TCGCTGACTACCGCATTCCATCAAACATTTGGTGAGGGAGATCGTTGTGATCTGGATACCACCTACCGTTTTAATAGTCG
TATCGGCGAAGTCGCTAATCGGTTTATTCAGCAGAATCCGCATCAACTTAAAAAACCGCTTAATAGCCTGATGAGCGGTG
ATAAAAAATCGGTGACCTTGCTTGAGGAAGGACAACTGGATGCACTGTTAGATAAACTTTCCGGTTATGCCAAACCTGAA
GAACGTATCCTCGTACTGGCGAGATATCATCACCTTAAACCAGCGAGTCTCGAAAAAGCACAAACTCGCTGGCCGAAGTT
GCAAATCGATTTTATGACCATCCACGCTAGTAAAGGCCAGCAGGCTGACTATGTCATTATTGTCGGATTGCACGAAGGGA
ATGATGGTTTCCCGGCCCCGGCGCGTGAATCAATCATTGAAGAGGCGTTACTTCCTCCTGTTGAAGACTTTGCTGATGCA
GAGGAACGGCGTTTGTTATATGTAGCGCTTACGCGTGCGCGCCAGCGTGTCTGGGCTTTATTTAATAAAGCCTCACCGTC
ATCGTTTGTGCAAAGTTTAAAAGATCTGGATGTGCCGATTGCCAGAAAACCATGA

Upstream 100 bases:

>100_bases
TTCTCTGCCAGGCGCAATTGTAACGTTTTTTTGGGCTGGAGCACGTTTTCTCTGGCGGTTAAACTGATATGAAAATCTCA
TCATGTGGATCAACTGAGTC

Downstream 100 bases:

>100_bases
ATAAGCAGGCGGTGGTGACCGCCTGCACATTTTATTTCAGGCGTTCTGCGAGATAACGCTGATAATCAGGGATGAGAATA
TCCACCGGATCATTAAAGTG

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MELKATSLGKRLAQHPYDRAVILNAGVKVSGDRHEYLIPFNHLLAINCKRGLVWGELEFVLPDEKVVRLHGTEWAETQRF
YHHLNAHWQQWSDEMSQIAATVLQEQLALITQQTGENKWLTRDKTQGIQQQIRQAFSALPLPVSRLESFDNCRDPLNQCM
AWLKDIEGSRRQHNQRYTEAMIKENAEFFQQIESSPLNPAQTRAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAA
AEQILLLAFGRKAAEEMDERIRQRLHTEDITARTFHSLALYIIQQGSKKLPTVSKLENDTQARHELFIKEWRKQCSEKKA
QAKGWRLWLEDELQWDVPEGNFWDDTKLQRRLASRLDRWVSLMRMHGGAQAEMIAGAPEDIRDLFSKRIKLLAPLLKAWK
SALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTSLFAVGDDWQAIYRFSGAQL
SLTTAFHQTFGEGDRCDLDTTYRFNSRIGEVANRFIQQNPHQLKKPLNSLMSGDKKSVTLLEEGQLDALLDKLSGYAKPE
ERILVLARYHHLKPASLEKAQTRWPKLQIDFMTIHASKGQQADYVIIVGLHEGNDGFPAPARESIIEEALLPPVEDFADA
EERRLLYVALTRARQRVWALFNKASPSSFVQSLKDLDVPIARKP

Sequences:

>Translated_684_residues
MELKATSLGKRLAQHPYDRAVILNAGVKVSGDRHEYLIPFNHLLAINCKRGLVWGELEFVLPDEKVVRLHGTEWAETQRF
YHHLNAHWQQWSDEMSQIAATVLQEQLALITQQTGENKWLTRDKTQGIQQQIRQAFSALPLPVSRLESFDNCRDPLNQCM
AWLKDIEGSRRQHNQRYTEAMIKENAEFFQQIESSPLNPAQTRAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAA
AEQILLLAFGRKAAEEMDERIRQRLHTEDITARTFHSLALYIIQQGSKKLPTVSKLENDTQARHELFIKEWRKQCSEKKA
QAKGWRLWLEDELQWDVPEGNFWDDTKLQRRLASRLDRWVSLMRMHGGAQAEMIAGAPEDIRDLFSKRIKLLAPLLKAWK
SALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTSLFAVGDDWQAIYRFSGAQL
SLTTAFHQTFGEGDRCDLDTTYRFNSRIGEVANRFIQQNPHQLKKPLNSLMSGDKKSVTLLEEGQLDALLDKLSGYAKPE
ERILVLARYHHLKPASLEKAQTRWPKLQIDFMTIHASKGQQADYVIIVGLHEGNDGFPAPARESIIEEALLPPVEDFADA
EERRLLYVALTRARQRVWALFNKASPSSFVQSLKDLDVPIARKP
>Mature_684_residues
MELKATSLGKRLAQHPYDRAVILNAGVKVSGDRHEYLIPFNHLLAINCKRGLVWGELEFVLPDEKVVRLHGTEWAETQRF
YHHLNAHWQQWSDEMSQIAATVLQEQLALITQQTGENKWLTRDKTQGIQQQIRQAFSALPLPVSRLESFDNCRDPLNQCM
AWLKDIEGSRRQHNQRYTEAMIKENAEFFQQIESSPLNPAQTRAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAA
AEQILLLAFGRKAAEEMDERIRQRLHTEDITARTFHSLALYIIQQGSKKLPTVSKLENDTQARHELFIKEWRKQCSEKKA
QAKGWRLWLEDELQWDVPEGNFWDDTKLQRRLASRLDRWVSLMRMHGGAQAEMIAGAPEDIRDLFSKRIKLLAPLLKAWK
SALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTSLFAVGDDWQAIYRFSGAQL
SLTTAFHQTFGEGDRCDLDTTYRFNSRIGEVANRFIQQNPHQLKKPLNSLMSGDKKSVTLLEEGQLDALLDKLSGYAKPE
ERILVLARYHHLKPASLEKAQTRWPKLQIDFMTIHASKGQQADYVIIVGLHEGNDGFPAPARESIIEEALLPPVEDFADA
EERRLLYVALTRARQRVWALFNKASPSSFVQSLKDLDVPIARKP

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=684, Percent_Identity=86.8421052631579, Blast_Score=1243, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=341, Percent_Identity=29.6187683284457, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI2367296, Length=338, Percent_Identity=29.585798816568, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77931; Mature: 77931

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKATSLGKRLAQHPYDRAVILNAGVKVSGDRHEYLIPFNHLLAINCKRGLVWGELEFV
CCCCHHHHHHHHHHCCCCCEEEEECCEEECCCCCEEEECCCCEEEEECCCCCEEEEEEEE
LPDEKVVRLHGTEWAETQRFYHHLNAHWQQWSDEMSQIAATVLQEQLALITQQTGENKWL
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TRDKTQGIQQQIRQAFSALPLPVSRLESFDNCRDPLNQCMAWLKDIEGSRRQHNQRYTEA
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MIKENAEFFQQIESSPLNPAQTRAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAA
HHHHHHHHHHHHHCCCCCHHHHHEEECCCCEEEEEEECCCCCCEEEEEECCCCEECCHHH
AEQILLLAFGRKAAEEMDERIRQRLHTEDITARTFHSLALYIIQQGSKKLPTVSKLENDT
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHH
QARHELFIKEWRKQCSEKKAQAKGWRLWLEDELQWDVPEGNFWDDTKLQRRLASRLDRWV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCHHHHHHHHHHHHHHHH
SLMRMHGGAQAEMIAGAPEDIRDLFSKRIKLLAPLLKAWKSALKAENAVDFSGLIHQAIV
HHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTSLFAVGDDWQAIYRFSGAQL
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCEE
SLTTAFHQTFGEGDRCDLDTTYRFNSRIGEVANRFIQQNPHQLKKPLNSLMSGDKKSVTL
EHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEE
LEEGQLDALLDKLSGYAKPEERILVLARYHHLKPASLEKAQTRWPKLQIDFMTIHASKGQ
ECCCCHHHHHHHHCCCCCCHHHEEEEEHHCCCCCCHHHHHHHCCCEEEEEEEEEECCCCC
QADYVIIVGLHEGNDGFPAPARESIIEEALLPPVEDFADAEERRLLYVALTRARQRVWAL
CCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHCEEHHHHHHHHHHHHHHH
FNKASPSSFVQSLKDLDVPIARKP
HHCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MELKATSLGKRLAQHPYDRAVILNAGVKVSGDRHEYLIPFNHLLAINCKRGLVWGELEFV
CCCCHHHHHHHHHHCCCCCEEEEECCEEECCCCCEEEECCCCEEEEECCCCCEEEEEEEE
LPDEKVVRLHGTEWAETQRFYHHLNAHWQQWSDEMSQIAATVLQEQLALITQQTGENKWL
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TRDKTQGIQQQIRQAFSALPLPVSRLESFDNCRDPLNQCMAWLKDIEGSRRQHNQRYTEA
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MIKENAEFFQQIESSPLNPAQTRAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAA
HHHHHHHHHHHHHCCCCCHHHHHEEECCCCEEEEEEECCCCCCEEEEEECCCCEECCHHH
AEQILLLAFGRKAAEEMDERIRQRLHTEDITARTFHSLALYIIQQGSKKLPTVSKLENDT
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHH
QARHELFIKEWRKQCSEKKAQAKGWRLWLEDELQWDVPEGNFWDDTKLQRRLASRLDRWV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCHHHHHHHHHHHHHHHH
SLMRMHGGAQAEMIAGAPEDIRDLFSKRIKLLAPLLKAWKSALKAENAVDFSGLIHQAIV
HHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTSLFAVGDDWQAIYRFSGAQL
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCEE
SLTTAFHQTFGEGDRCDLDTTYRFNSRIGEVANRFIQQNPHQLKKPLNSLMSGDKKSVTL
EHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEE
LEEGQLDALLDKLSGYAKPEERILVLARYHHLKPASLEKAQTRWPKLQIDFMTIHASKGQ
ECCCCHHHHHHHHCCCCCCHHHEEEEEHHCCCCCCHHHHHHHCCCEEEEEEEEEECCCCC
QADYVIIVGLHEGNDGFPAPARESIIEEALLPPVEDFADAEERRLLYVALTRARQRVWAL
CCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHCEEHHHHHHHHHHHHHHH
FNKASPSSFVQSLKDLDVPIARKP
HHCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]