Click here to switch to the map view.

The map label for this gene is yliE [H]

Identifier: 218548352

GI number: 218548352

Start: 1005071

End: 1007419

Strand: Direct

Name: yliE [H]

Synonym: EFER_0974

Alternate gene names: 218548352

Gene position: 1005071-1007419 (Clockwise)

Preceding gene: 218548351

Following gene: 218548353

Centisome position: 21.9

GC content: 40.14

Gene sequence:

>2349_bases
ATGCATAGTTTGTACGAAAAGATTAAGATTAGATTGATAATTCTTTTTCTACTGGCTGCGCTATTATTTATTGGCCTGTT
TTATGTATTAAACCTTGAACTGGTTTCTGACAGAGCCATAAAAAATGCTGAAAACAGATTTGAGCTAATTGAAAAGAATA
TTGGCTATTTTTTTAACGATATTGAAAGAGATGCCCTAACACTTAAAGATTCTCTTTATATGCTACGTAGAGAAGATGAG
ATTATCCGCGCAGCATTATTAAAAATGGAATCAATGCCTTATCTCGATACCATTGGTTTGGTCATGGATAACGGTAAATA
TTTACTCTTTACCCGTGGTGTGAATAATAAAATAAATGTTTATCACCAGAATAAAATCAACGGACCGTTAGTTGATCAGG
CAGGCAGGGAGGTCTTTAAACAGTTTAATCCTAATAAACGCCCGTGGTCGGAAACGTTAGTCAATGAAAATAGTGGCTGG
AGTGAAGCTTATAATTGCTTCGAACGTCCGGGTAAAAAATGTATCTCATGGACATTAGCCATTAACGGTAAAGATCGAGA
GCTACTGGCAATTGACAGAATACATGTTGATCTGAACTGGCGACACTTAAATGAGTATCTTGATAAAATCAGCAGTGGTG
GTGAAGTTCTTTTCCTGCAACAGGGTAACGTGGTTATCGCCAAAAACAACAACGCACGAGAAAAACTGATCGTTTATAAT
GGAGAAGGTAATTATCACATTATAGACTCGAACGACACCTATTATATTTCACAACGCGCTCAGGTGCCGAATCATCCACT
ATTTGATATTTATTTTTATTATCCAATCGGGAGTTTATTCAGTACTGCAGATAAATATGTCTATATCTCCTTTGTCTTTA
TTATTGCAGTATTGGTAGTGCTGTATGCAATAAGTGTCAGGATCTTGCGTGGGCAGTTTTCAGAAATGACCTCACTGGTA
ACCGCGCTGGAATTCTTACCAGACTCTGCAGACGAAAACCAGGCATTAAAAATTCATGATGGCGATGCGAAAGAGATAAT
CAGTATTAAAAATTCGATTGCTGAAATGAAAGGGGCGGAAATTGAGCGCTCGAATAAACTACTGTCACTGATCTCTTATG
ATCAGGAAAGTGGCTTTATAAAGAATATGGCGATTATTGAATCGAATAATCGTCAGTACCTGGGGGCCGGGATGATTAAA
TTCTGCGGTCTGGAATCTCTGGAGGCTGTTTTTGGTGTAGAAGAGCGTAACAAAGTCGTCAAAGTTCTCTGCCGCCGTAT
TGCCGAAAAATTTGCTCAAAGTTGCGATATTGTCACGCTTTCCAGTGATCTCTACTTCCTGTTATGCCGGGAAAATGTGC
GTGCTTTTGCGAATAACATTGCCCTGGTTGAGACGTTTGAAAAAGGTCTGGGTTACCGGAATATCCGTATTCATAATTCG
GCGATTTATGAGTTTCTCAAAGGTGAGGCTGCTTACGGTTACGTTGAGAAACTCAAACTGGCCCTATCCAGTATTCGCAA
TCATATGTTCTCTGAGTTCATTCTTTGCGATAACGATAAACTTAATGAAATAGAAGAGAACATCTGGATCTCACGTAATA
TTCGTCGGGCAATGAAAGAGGGCGAACTTTTCCTCGTTTACCAGCCTATTGTTGATATTCGTAGCCAGGGGATCCTTGGG
GCAGAAGCACTCTGCCGTTGGGTGTCTGAGGAACGGGGTAATATTTCGCCACTGAAATTTATTTCGATTGCAGAAGATAT
CGGTTTTATTAATGAGTTAGGGAACTGGATCATTGAGACCGCTATGCACGAATTTGCGGAGTTCAGGGCAAAGGCGCAAC
TGCGGGAAGATTTCCATCTGCATATTAATGTCTCGCCCTGGCAACTTAATGAACCTCTGTTCTATGAACGGATTATGGCG
TGTCTCAGCAGTAGTGGTCTGCAACCGCAACAGATTTGTATTGAGATCACTGAAACAGTGGTTGAACGTATTAATGACTA
TTTCTATCAGAATATTGCGGTATTCAGAGAACAAGGTTTTACCATTGCTATCGATGACTTCGGTACAGGTTTATCTAACC
TGAAACGCTTTTATGAGATTAATCCTGACAGCATCAAGATTGATTATGAGTTTACATCCGATATTTTTGGTACTGCGGGG
AAAATTATCCAGGTGATATTAGATCTTGCGCAAGATAACCATATTCCGGTTGTTGCAGAAGGTGTCGAAACACCAGAGAT
TGCCAGTGCGTTAATGGATATTGGCTGTGTACAGGCGCAGGGGTATCTGTATCAGAAACCGTTGCCATTTTCAGCATGGG
ATATCAGTGGAAAGTTAGTGAAAGAGTAA

Upstream 100 bases:

>100_bases
TTGTGTATTTTGCCTGATTTTGTAGAATCAGGCCATTTGATTAACCGCCGCGCCATACTTAGCGGTGTAATGAGTAACCA
ATACTTAGTTAATTATTGTC

Downstream 100 bases:

>100_bases
AAAAGGTTTATGTCCAGAATCAATAAAATCGTACTTATGGTGAGTCTGCTGATTTTTTTGTTAATATCGGCAGTCGCCTG
CGGAATCTATACCCAAATGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 782; Mature: 782

Protein sequence:

>782_residues
MHSLYEKIKIRLIILFLLAALLFIGLFYVLNLELVSDRAIKNAENRFELIEKNIGYFFNDIERDALTLKDSLYMLRREDE
IIRAALLKMESMPYLDTIGLVMDNGKYLLFTRGVNNKINVYHQNKINGPLVDQAGREVFKQFNPNKRPWSETLVNENSGW
SEAYNCFERPGKKCISWTLAINGKDRELLAIDRIHVDLNWRHLNEYLDKISSGGEVLFLQQGNVVIAKNNNAREKLIVYN
GEGNYHIIDSNDTYYISQRAQVPNHPLFDIYFYYPIGSLFSTADKYVYISFVFIIAVLVVLYAISVRILRGQFSEMTSLV
TALEFLPDSADENQALKIHDGDAKEIISIKNSIAEMKGAEIERSNKLLSLISYDQESGFIKNMAIIESNNRQYLGAGMIK
FCGLESLEAVFGVEERNKVVKVLCRRIAEKFAQSCDIVTLSSDLYFLLCRENVRAFANNIALVETFEKGLGYRNIRIHNS
AIYEFLKGEAAYGYVEKLKLALSSIRNHMFSEFILCDNDKLNEIEENIWISRNIRRAMKEGELFLVYQPIVDIRSQGILG
AEALCRWVSEERGNISPLKFISIAEDIGFINELGNWIIETAMHEFAEFRAKAQLREDFHLHINVSPWQLNEPLFYERIMA
CLSSSGLQPQQICIEITETVVERINDYFYQNIAVFREQGFTIAIDDFGTGLSNLKRFYEINPDSIKIDYEFTSDIFGTAG
KIIQVILDLAQDNHIPVVAEGVETPEIASALMDIGCVQAQGYLYQKPLPFSAWDISGKLVKE

Sequences:

>Translated_782_residues
MHSLYEKIKIRLIILFLLAALLFIGLFYVLNLELVSDRAIKNAENRFELIEKNIGYFFNDIERDALTLKDSLYMLRREDE
IIRAALLKMESMPYLDTIGLVMDNGKYLLFTRGVNNKINVYHQNKINGPLVDQAGREVFKQFNPNKRPWSETLVNENSGW
SEAYNCFERPGKKCISWTLAINGKDRELLAIDRIHVDLNWRHLNEYLDKISSGGEVLFLQQGNVVIAKNNNAREKLIVYN
GEGNYHIIDSNDTYYISQRAQVPNHPLFDIYFYYPIGSLFSTADKYVYISFVFIIAVLVVLYAISVRILRGQFSEMTSLV
TALEFLPDSADENQALKIHDGDAKEIISIKNSIAEMKGAEIERSNKLLSLISYDQESGFIKNMAIIESNNRQYLGAGMIK
FCGLESLEAVFGVEERNKVVKVLCRRIAEKFAQSCDIVTLSSDLYFLLCRENVRAFANNIALVETFEKGLGYRNIRIHNS
AIYEFLKGEAAYGYVEKLKLALSSIRNHMFSEFILCDNDKLNEIEENIWISRNIRRAMKEGELFLVYQPIVDIRSQGILG
AEALCRWVSEERGNISPLKFISIAEDIGFINELGNWIIETAMHEFAEFRAKAQLREDFHLHINVSPWQLNEPLFYERIMA
CLSSSGLQPQQICIEITETVVERINDYFYQNIAVFREQGFTIAIDDFGTGLSNLKRFYEINPDSIKIDYEFTSDIFGTAG
KIIQVILDLAQDNHIPVVAEGVETPEIASALMDIGCVQAQGYLYQKPLPFSAWDISGKLVKE
>Mature_782_residues
MHSLYEKIKIRLIILFLLAALLFIGLFYVLNLELVSDRAIKNAENRFELIEKNIGYFFNDIERDALTLKDSLYMLRREDE
IIRAALLKMESMPYLDTIGLVMDNGKYLLFTRGVNNKINVYHQNKINGPLVDQAGREVFKQFNPNKRPWSETLVNENSGW
SEAYNCFERPGKKCISWTLAINGKDRELLAIDRIHVDLNWRHLNEYLDKISSGGEVLFLQQGNVVIAKNNNAREKLIVYN
GEGNYHIIDSNDTYYISQRAQVPNHPLFDIYFYYPIGSLFSTADKYVYISFVFIIAVLVVLYAISVRILRGQFSEMTSLV
TALEFLPDSADENQALKIHDGDAKEIISIKNSIAEMKGAEIERSNKLLSLISYDQESGFIKNMAIIESNNRQYLGAGMIK
FCGLESLEAVFGVEERNKVVKVLCRRIAEKFAQSCDIVTLSSDLYFLLCRENVRAFANNIALVETFEKGLGYRNIRIHNS
AIYEFLKGEAAYGYVEKLKLALSSIRNHMFSEFILCDNDKLNEIEENIWISRNIRRAMKEGELFLVYQPIVDIRSQGILG
AEALCRWVSEERGNISPLKFISIAEDIGFINELGNWIIETAMHEFAEFRAKAQLREDFHLHINVSPWQLNEPLFYERIMA
CLSSSGLQPQQICIEITETVVERINDYFYQNIAVFREQGFTIAIDDFGTGLSNLKRFYEINPDSIKIDYEFTSDIFGTAG
KIIQVILDLAQDNHIPVVAEGVETPEIASALMDIGCVQAQGYLYQKPLPFSAWDISGKLVKE

Specific function: Overexpression reduces biofilm formation. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787055, Length=782, Percent_Identity=69.5652173913043, Blast_Score=1122, Evalue=0.0,
Organism=Escherichia coli, GI87081921, Length=256, Percent_Identity=31.640625, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1790496, Length=247, Percent_Identity=33.6032388663968, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1787541, Length=251, Percent_Identity=32.2709163346614, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI87081845, Length=239, Percent_Identity=33.4728033472803, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI87081980, Length=249, Percent_Identity=34.5381526104418, Blast_Score=118, Evalue=2e-27,
Organism=Escherichia coli, GI1788502, Length=242, Percent_Identity=34.7107438016529, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1788849, Length=250, Percent_Identity=30.8, Blast_Score=109, Evalue=9e-25,
Organism=Escherichia coli, GI87081743, Length=241, Percent_Identity=30.7053941908714, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI87082096, Length=252, Percent_Identity=28.968253968254, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI226510982, Length=266, Percent_Identity=26.6917293233083, Blast_Score=97, Evalue=6e-21,
Organism=Escherichia coli, GI1786507, Length=248, Percent_Identity=28.6290322580645, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787410, Length=236, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633 [H]

Pfam domain/function: PF00563 EAL [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 89589; Mature: 89589

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSLYEKIKIRLIILFLLAALLFIGLFYVLNLELVSDRAIKNAENRFELIEKNIGYFFND
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
IERDALTLKDSLYMLRREDEIIRAALLKMESMPYLDTIGLVMDNGKYLLFTRGVNNKINV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCEEEEECCCCCCEEE
YHQNKINGPLVDQAGREVFKQFNPNKRPWSETLVNENSGWSEAYNCFERPGKKCISWTLA
EECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEE
INGKDRELLAIDRIHVDLNWRHLNEYLDKISSGGEVLFLQQGNVVIAKNNNAREKLIVYN
ECCCCCEEEEEEEEEEEECHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCEEEEEEE
GEGNYHIIDSNDTYYISQRAQVPNHPLFDIYFYYPIGSLFSTADKYVYISFVFIIAVLVV
CCCCEEEECCCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHH
LYAISVRILRGQFSEMTSLVTALEFLPDSADENQALKIHDGDAKEIISIKNSIAEMKGAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCC
IERSNKLLSLISYDQESGFIKNMAIIESNNRQYLGAGMIKFCGLESLEAVFGVEERNKVV
HHHCCHHHHHHCCCCCCCCEEEEEEEECCCCEEECCCHHHHCCHHHHHHHHCHHHHHHHH
KVLCRRIAEKFAQSCDIVTLSSDLYFLLCRENVRAFANNIALVETFEKGLGYRNIRIHNS
HHHHHHHHHHHHCCCCEEEECCCEEEEEEHHHHHHHHHCHHHEEHHHHCCCCCEEEEEHH
AIYEFLKGEAAYGYVEKLKLALSSIRNHMFSEFILCDNDKLNEIEENIWISRNIRRAMKE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHC
GELFLVYQPIVDIRSQGILGAEALCRWVSEERGNISPLKFISIAEDIGFINELGNWIIET
CCEEEEEECHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AMHEFAEFRAKAQLREDFHLHINVSPWQLNEPLFYERIMACLSSSGLQPQQICIEITETV
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VERINDYFYQNIAVFREQGFTIAIDDFGTGLSNLKRFYEINPDSIKIDYEFTSDIFGTAG
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHEECCCCEEEEEEEECCHHHHHHH
KIIQVILDLAQDNHIPVVAEGVETPEIASALMDIGCVQAQGYLYQKPLPFSAWDISGKLV
HHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHEECCCEEEECCCCCCEECCCCCEE
KE
CC
>Mature Secondary Structure
MHSLYEKIKIRLIILFLLAALLFIGLFYVLNLELVSDRAIKNAENRFELIEKNIGYFFND
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
IERDALTLKDSLYMLRREDEIIRAALLKMESMPYLDTIGLVMDNGKYLLFTRGVNNKINV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCEEEEECCCCCCEEE
YHQNKINGPLVDQAGREVFKQFNPNKRPWSETLVNENSGWSEAYNCFERPGKKCISWTLA
EECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEE
INGKDRELLAIDRIHVDLNWRHLNEYLDKISSGGEVLFLQQGNVVIAKNNNAREKLIVYN
ECCCCCEEEEEEEEEEEECHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCEEEEEEE
GEGNYHIIDSNDTYYISQRAQVPNHPLFDIYFYYPIGSLFSTADKYVYISFVFIIAVLVV
CCCCEEEECCCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHH
LYAISVRILRGQFSEMTSLVTALEFLPDSADENQALKIHDGDAKEIISIKNSIAEMKGAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCC
IERSNKLLSLISYDQESGFIKNMAIIESNNRQYLGAGMIKFCGLESLEAVFGVEERNKVV
HHHCCHHHHHHCCCCCCCCEEEEEEEECCCCEEECCCHHHHCCHHHHHHHHCHHHHHHHH
KVLCRRIAEKFAQSCDIVTLSSDLYFLLCRENVRAFANNIALVETFEKGLGYRNIRIHNS
HHHHHHHHHHHHCCCCEEEECCCEEEEEEHHHHHHHHHCHHHEEHHHHCCCCCEEEEEHH
AIYEFLKGEAAYGYVEKLKLALSSIRNHMFSEFILCDNDKLNEIEENIWISRNIRRAMKE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHC
GELFLVYQPIVDIRSQGILGAEALCRWVSEERGNISPLKFISIAEDIGFINELGNWIIET
CCEEEEEECHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AMHEFAEFRAKAQLREDFHLHINVSPWQLNEPLFYERIMACLSSSGLQPQQICIEITETV
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VERINDYFYQNIAVFREQGFTIAIDDFGTGLSNLKRFYEINPDSIKIDYEFTSDIFGTAG
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHEECCCCEEEEEEEECCHHHHHHH
KIIQVILDLAQDNHIPVVAEGVETPEIASALMDIGCVQAQGYLYQKPLPFSAWDISGKLV
HHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHEECCCEEEECCCCCCEECCCCCEE
KE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]