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The map label for this gene is alkB [H]

Identifier: 218548327

GI number: 218548327

Start: 975969

End: 976619

Strand: Direct

Name: alkB [H]

Synonym: EFER_0948

Alternate gene names: 218548327

Gene position: 975969-976619 (Clockwise)

Preceding gene: 218548326

Following gene: 218548328

Centisome position: 21.27

GC content: 51.77

Gene sequence:

>651_bases
ATGTTGGATTTATTTGCCAATGCAGAACCGTGGCAAGAATCGCTGGCGGATGGTGCGGTAATTTTGCGCCGTTTTGCTTT
TAACGTTGCGGAACAACTGATCCGCGATATTAATGACGTTGCCAGCCAGTCGCCGTTTCGCCAGATGGTCACTCCAGGCG
GATATACCATGTCGGTAGCGATGACCAACTGTGGGCATCTGGGCTGGACGACCAATCGGCAAGGTTATCTCTATTCGCCC
ATTGATCCGCAAACAAATAAACCATGGCCCGCCATGCCGCATAGTTTTCATGATTTATGTCAATGTGCGGCCACGGCGGC
GGGTTATCCAGATTTCCAGCCTGATGCTTGTCTTATCAACCGCTACGCTCCTGGCGCGAAACTGTCGCTGCATCAGGATA
AAGACGAACCGGATCTGCGCGCGCCGATTGTTTCTGTTTCTCTGGGCTTACCCGCGATTTTTCAATTTGGTGGCCTGAAA
CGAAATGATCCGCTCAAACGTTTGTTGCTGGAACATGGCGATGTGGTGGTGTGGGGCGGTGAATCGCGGCTGTTTTATCA
CGGCATTCAACCGTTGAAAGCGGGCTTTCATCCACTCACCGCCGACTGCCGCTACAACCTGACATTCCGTCAGGCAGGTA
AAAAAGAATAA

Upstream 100 bases:

>100_bases
ATCGGGTGGTCCATGGTGATGGCACACTTTCCGGTTACCGCTGGGGCGTGTCGCGCAAAGCGCAACTGCTGCGCCGCGAA
TCTGAACAAGAGGAGAGGTA

Downstream 100 bases:

>100_bases
AAATAAGAATTATTATTGCTGTGCGCGCGGAGATGTTTAAACTGCGGGCTGTAATTCATTGTCCGGGTTTTCTGCATGGA
ACTTCTTGTACTTGTCTGGC

Product: oxidative demethylase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLDLFANAEPWQESLADGAVILRRFAFNVAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTTNRQGYLYSP
IDPQTNKPWPAMPHSFHDLCQCAATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTADCRYNLTFRQAGKKE

Sequences:

>Translated_216_residues
MLDLFANAEPWQESLADGAVILRRFAFNVAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTTNRQGYLYSP
IDPQTNKPWPAMPHSFHDLCQCAATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTADCRYNLTFRQAGKKE
>Mature_216_residues
MLDLFANAEPWQESLADGAVILRRFAFNVAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTTNRQGYLYSP
IDPQTNKPWPAMPHSFHDLCQCAATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTADCRYNLTFRQAGKKE

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=98, Percent_Identity=33.6734693877551, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1788541, Length=216, Percent_Identity=95.8333333333333, Blast_Score=431, Evalue=1e-122,
Organism=Drosophila melanogaster, GI45555401, Length=98, Percent_Identity=41.8367346938776, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24029; Mature: 24029

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLFANAEPWQESLADGAVILRRFAFNVAEQLIRDINDVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEE
MTNCGHLGWTTNRQGYLYSPIDPQTNKPWPAMPHSFHDLCQCAATAAGYPDFQPDACLIN
ECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
RYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGG
CCCCCCEEEECCCCCCCCHHCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKAGFHPLTADCRYNLTFRQAGKKE
CCEEEEECCCHHHCCCCCCCCCCEECEEHHCCCCCC
>Mature Secondary Structure
MLDLFANAEPWQESLADGAVILRRFAFNVAEQLIRDINDVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEE
MTNCGHLGWTTNRQGYLYSPIDPQTNKPWPAMPHSFHDLCQCAATAAGYPDFQPDACLIN
ECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
RYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGG
CCCCCCEEEECCCCCCCCHHCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKAGFHPLTADCRYNLTFRQAGKKE
CCEEEEECCCHHHCCCCCCCCCCEECEEHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]