Click here to switch to the map view.

The map label for this gene is nuoM [H]

Identifier: 218548274

GI number: 218548274

Start: 908552

End: 910081

Strand: Direct

Name: nuoM [H]

Synonym: EFER_0893

Alternate gene names: 218548274

Gene position: 908552-910081 (Clockwise)

Preceding gene: 218548273

Following gene: 218548275

Centisome position: 19.8

GC content: 53.99

Gene sequence:

>1530_bases
ATGTTACTACCCTGGCTAATATTAATTCCCTTTATTGGCGGCTTCCTGTGCTGGCAGACCGAACGCTTTGGCGTCAAGGT
GCCACGCTGGATCGCGCTGATCACCATGGGATTGACGCTGGCGCTGTCGCTGCAACTGTGGTTGCAGGGCGGTTATTCAC
TGACGCAATCCGCCGGAATTCCGCAGTGGCAGTCTGAATTCGACATGCCATGGATCCCGCGTTTTGGTATCTCTATTCAT
CTCGCCATTGATGGTCTGTCGCTGCTGATGGTCGTGCTGACCGGTCTGCTCGGCGTGCTGGCGGTACTTTGTTCGTGGAA
AGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCA
TCGACATGTTCCTGTTCTTCTTTTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTTTGGGGGCATAAA
GCTTCTGACGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTACACCCAGGCGAGTGGTCTGGTGATGTTGAT
TGCTATCCTGGCGCTGGTTTTTGTTCACTACAATGCGACCGGTGTCTGGACGTTCAATTATGAAGAGCTGCTGAATACGC
CGATGTCTAACGGCGTGGAATATCTGTTGATGCTGGGCTTCTTCATCGCCTTCGCAGTGAAAATGCCGGTGGTTCCGCTG
CATGGCTGGCTGCCGGATGCGCACTCTCAGGCACCGACTGCCGGTTCCGTTGACCTCGCGGGGATCTTGCTGAAAACCGC
CGCTTACGGTTTGCTGCGTTTCTCTCTGCCACTGTTTCCGAACGCGTCGGCAGAGTTCGCGCCAATCGCCATGTGGTTGG
GTGTTATCGGCATCTTCTACGGTGCGTGGATGGCCTTCGCCCAGACCGATATCAAACGTCTGATCGCCTACACCTCGGTT
TCCCACATGGGCTTCGTGCTGATTGCTATCTACACCGGCAGCCAGTTGGCCTACCAGGGCGCGGTCATTCAGATGATTGC
TCACGGCTTGTCGGCGGCGGGTCTGTTTATTCTCTGCGGTCAGCTTTATGAGCGTATCCATACCCGCGATATGCGCATGA
TGGGCGGTCTGTGGAGCAAAATGAAATGGCTGCCAGCACTGTCGCTGTTCTTTGCAGTGGCAACGCTTGGGATGCCAGGC
ACCGGTAACTTCGTCGGCGAATTTATGATCCTGTTCGGCAGCTTCCAGGTTGTCCCGATGATTACCGTTATCTCTACCTT
CGGGCTGGTCTTTGCTTCTGTTTATTCGCTGGCGATGCTTCATCGCGCTTACTTCGGTAAAGCGAAAAGCGAGATTGCCA
GCAAAGAACTGCCAGGGATGTCGCTGCGTGAGCTGTTTATGATCCTGTTGCTGGTGGTGCTGCTGGTACTGCTGGGCTTC
TATCCGCAGCCGATTCTGGATACCTCGCATTCCGCGATTGGCAATATCCAGCAGTGGTTTGTTAATTCCGTTACTACTAC
AAGGCCGTAA

Upstream 100 bases:

>100_bases
GTGTATCCGACACTACCCCCGGTCCGAAGAGAATTTTCTGTCGTGAGAATTCGTTGAAAATCCGGTCCTGACGGGACTTT
TACAAGGAATAAAGATCGCC

Downstream 100 bases:

>100_bases
ATCGCCATGACAATAACTCCACAAAACCTGATCGCACTGCTACCGTTGCTGATCGTCGGCTTGACGGTGGTGGTTGTGAT
GCTCTCCATTGCGTGGCGAC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M; NUO13 [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIH
LAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPG
TGNFVGEFMILFGSFQVVPMITVISTFGLVFASVYSLAMLHRAYFGKAKSEIASKELPGMSLRELFMILLLVVLLVLLGF
YPQPILDTSHSAIGNIQQWFVNSVTTTRP

Sequences:

>Translated_509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIH
LAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPG
TGNFVGEFMILFGSFQVVPMITVISTFGLVFASVYSLAMLHRAYFGKAKSEIASKELPGMSLRELFMILLLVVLLVLLGF
YPQPILDTSHSAIGNIQQWFVNSVTTTRP
>Mature_509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIH
LAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPG
TGNFVGEFMILFGSFQVVPMITVISTFGLVFASVYSLAMLHRAYFGKAKSEIASKELPGMSLRELFMILLLVVLLVLLGF
YPQPILDTSHSAIGNIQQWFVNSVTTTRP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=368, Percent_Identity=33.4239130434783, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI251831117, Length=271, Percent_Identity=28.0442804428044, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1788613, Length=509, Percent_Identity=99.2141453831041, Blast_Score=1009, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=452, Percent_Identity=25.4424778761062, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1788831, Length=497, Percent_Identity=25.7545271629779, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1788614, Length=494, Percent_Identity=25.7085020242915, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693160, Length=432, Percent_Identity=25.462962962963, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788829, Length=234, Percent_Identity=26.4957264957265, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56586; Mature: 56586

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGI
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
PQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNL
CCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHH
MWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNGVEYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHH
ITVISTFGLVFASVYSLAMLHRAYFGKAKSEIASKELPGMSLRELFMILLLVVLLVLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
YPQPILDTSHSAIGNIQQWFVNSVTTTRP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGI
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
PQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNL
CCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHH
MWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNGVEYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHH
ITVISTFGLVFASVYSLAMLHRAYFGKAKSEIASKELPGMSLRELFMILLLVVLLVLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
YPQPILDTSHSAIGNIQQWFVNSVTTTRP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]