Click here to switch to the map view.

The map label for this gene is pdxB [H]

Identifier: 218548225

GI number: 218548225

Start: 860153

End: 861289

Strand: Direct

Name: pdxB [H]

Synonym: EFER_0843

Alternate gene names: 218548225

Gene position: 860153-861289 (Clockwise)

Preceding gene: 218548223

Following gene: 218548226

Centisome position: 18.74

GC content: 52.33

Gene sequence:

>1137_bases
GTGAAAATCCTTGTTGATGAAAATATGCCTTATGCCCGCGAATTATTTAGTCGCCTGGGGGAAGTGAAAGCAGTTCCTGG
CCGCCCGATTCCCGTCGCTCAACTGGCAGACGCGGATGCGCTGATGGTGCGTTCGGTCACCAAAGTGAATGAATCTTTGC
TGGCAGGAAAACCCATTAAATTTGTTGGCACTGCCACAGCGGGGACCGACCATGTCGATGAAGCATGGTTAAAGCAGGCG
GGAATTGGTTTTTCCGCAGCACCTGGCTGTAATGCGATTGCGGTGGTGGAATATGTTTTCTCCTCACTGCTGATGCTTGC
TGAACGCGATGGATTTTCACTGCATGAGCGTACCGTGGGGATCGTGGGCGTTGGTAACGTTGGTCGGCGTTTACAGGCAC
GACTGGAAGCGCTGGGGATCAAAACCTTGCTTTGCGATCCGCCGCGTGCCGATCGTGGGGATGAGGGCGATTTCCGTTCG
TTGGATGAATTAGTCCAGCATGCGGATATCCTGACATTCCATACGCCACTCTTTAAAGATGGACCGTACAAAACGCTACA
TCTGGCGGATGAAAAACTGATCCGCAGCCTGAAGCCGGGAGCGATCTTGATTAACGCCTGCCGTGGCGCAGTCGTCGATA
ATACTGCCCTGTTGACCTGTCTGAATGAAGGGCAGAAGTTAAGCGTAGTGCTGGATGTCTGGGAAGGCGAACCGGAACTC
AACGTAGAATTGCTGAAAAAAGTGGATATCGGCACGCCGCATATTGCGGGTTACACCCTCGAAGGTAAAGCCCGTGGCAC
GACGCAAGTGTTTGAAGCCTATAGCAAGTTTATTGGGCATGAACAACACGTTGCGCTGGATACGTTGCTACCCGCACCAG
AGTTTGGTCGCATTACGCTGCATGGTCTGCTCGATCAACCGACGCTGAAAAGGCTGGTGCATTTGGTGTATGATGTGCGC
CGCGATGACGCACCGCTGCGTAAAGTCGCCGGAATACCGGGTGAGTTCGATAAACTGCGCAAAAACTATCTTGAGCGCCG
TGAATGGTCATCTCTGTATGTGATTTGTGATGACGCCAGTGCGGCATCATTGCTGTGTAAACTGGGTTTTAACGCCGTTC
ATCATCCGGCACGTTAA

Upstream 100 bases:

>100_bases
TCACTTATCCTTAAATCATAAATCTTCGTAAGTATGCCTGACGGCGAGATTATGGCACACTTGCCCGGTTAACTCTCGTT
CTCAAACAGGTAACACAAAC

Downstream 100 bases:

>100_bases
TCTCTTCTTCATGCTCTCTGCTGTAACATTGGCAGAGAGCTTTGCTATTTCTGGAGTAAACCACCATGTCTGAAGGCTGG
AACATTGCCGTCCTGGGCGC

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKILVDENMPYARELFSRLGEVKAVPGRPIPVAQLADADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
NVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGLLDQPTLKRLVHLVYDVR
RDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR

Sequences:

>Translated_378_residues
MKILVDENMPYARELFSRLGEVKAVPGRPIPVAQLADADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
NVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGLLDQPTLKRLVHLVYDVR
RDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR
>Mature_378_residues
MKILVDENMPYARELFSRLGEVKAVPGRPIPVAQLADADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
NVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGLLDQPTLKRLVHLVYDVR
RDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=257, Percent_Identity=30.3501945525292, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI61743967, Length=253, Percent_Identity=26.8774703557312, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4557497, Length=253, Percent_Identity=26.8774703557312, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI145580578, Length=276, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4557499, Length=276, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI145580575, Length=276, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI6912396, Length=316, Percent_Identity=26.2658227848101, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788660, Length=378, Percent_Identity=98.1481481481482, Blast_Score=754, Evalue=0.0,
Organism=Escherichia coli, GI1787645, Length=205, Percent_Identity=25.8536585365854, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789279, Length=305, Percent_Identity=28.1967213114754, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082289, Length=259, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=263, Percent_Identity=28.1368821292776, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320925, Length=359, Percent_Identity=27.2980501392758, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322116, Length=359, Percent_Identity=27.0194986072423, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=300, Percent_Identity=27, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324964, Length=264, Percent_Identity=26.1363636363636, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921140, Length=273, Percent_Identity=26.007326007326, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24646446, Length=253, Percent_Identity=26.8774703557312, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646448, Length=253, Percent_Identity=26.8774703557312, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646452, Length=253, Percent_Identity=26.8774703557312, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646450, Length=253, Percent_Identity=26.8774703557312, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62472511, Length=253, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 41404; Mature: 41404

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVDENMPYARELFSRLGEVKAVPGRPIPVAQLADADALMVRSVTKVNESLLAGKPIK
CEEEECCCCCHHHHHHHHHCCEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
FVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHERTVG
EEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCEEE
IVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRSLDELVQHADILTFHTPLFKD
EEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCC
GPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
CCCCEEEHHHHHHHHHCCCCEEEEECCCCCEECCCEEEEEECCCCEEEEEEEEECCCCCC
NVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITL
CHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHEEHHHHCCCCCCCCEEE
HGLLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDAS
EECCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCH
AASLLCKLGFNAVHHPAR
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKILVDENMPYARELFSRLGEVKAVPGRPIPVAQLADADALMVRSVTKVNESLLAGKPIK
CEEEECCCCCHHHHHHHHHCCEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
FVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHERTVG
EEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCEEE
IVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRSLDELVQHADILTFHTPLFKD
EEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCC
GPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
CCCCEEEHHHHHHHHHCCCCEEEEECCCCCEECCCEEEEEECCCCEEEEEEEEECCCCCC
NVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITL
CHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHEEHHHHCCCCCCCCEEE
HGLLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDAS
EECCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCH
AASLLCKLGFNAVHHPAR
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA