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The map label for this gene is yfeG [H]

Identifier: 218548121

GI number: 218548121

Start: 752061

End: 753113

Strand: Direct

Name: yfeG [H]

Synonym: EFER_0733

Alternate gene names: 218548121

Gene position: 752061-753113 (Clockwise)

Preceding gene: 218548120

Following gene: 218548126

Centisome position: 16.39

GC content: 52.42

Gene sequence:

>1053_bases
ATGAAAAAGACCCGTACAGCCAATTTGCACCATCTTTATCATGAACCCTTACCCGAAAACCTGAAGCTCACGCCGAAGGT
CGAAGTGGATAATGTTCATCAACGACAGACAACGGATGTCTATGAACATGCTTTGACGATTACCGCCTGGCAGCAGATTT
ACGATCAGCTTCATCCGGGCAAATTTCATGGTGAATTTACGGAAATTCTGCTCGATGATATTCAGGTTTTCCGTGAATAC
ACGGGGCTGGCGCTGCGTCAGTCGTGCCTGGTCTGGCCGAACTCGTTCTGGTTTGGCATTCCGGCGACGCGCGGTGAGCA
GGGATTTATCGGTTCGCAATGTCTGGGAAGCGCAGAAATTGCGACGCGCCCTGGAGGAACCGAGTTTGAATTAAGTACGC
CGGACGATTACACGATCCTTGGCGTGGTGCTTTCTGAAGATGTCATCACCCGGCAGGCTAACTTTTTGCATAACCCGGAA
AGGGTGCTGCATATGTTACGTAACCAGTCGGCGCTGGAAGTGAAAGAGCAGCATAAAGCCGCGCTGTGGGGCTTTATCCA
ACAGGCTCTGGCGACATTTTGCGAGAACCCGGAAAATCTCCATCAGCCTGCAGTGCGAAAAGTGCTGGGGGATAATTTGC
TAATGGCGATGGGGGCGATGCTGGAAGAAGCGCAGCCAATGGTGACGGTGGAAAGCATCAGTCATCAGAGTTACCGTCGG
TTGCTTTCCCGCGCCCGTGAATATGTGCTGGAAAACATGTCCGAACCGGTGACGGTGCTGGATTTGTGTAATCAACTGCA
CGTCAGTCGCCGCACCTTGCAAAACGCATTTCACGCTATTTTGGGCATTGGCCCAAACGCGTGGCTGAAACGCATTCGCC
TGAACGCCGTGCGCCGTGAACTGATTAGCCCGTGGTCGCAAAGTATGACGGTAAAAGACGCCGCCATGCAGTGGGGATTC
TGGCATCTTGGGCAGTTTGCCACGGATTATCAGCAATTGTTTGCAGAAAAGCCTTCACTGACGCTGCATCAGCGGATGCA
GGAACTGGAGTGA

Upstream 100 bases:

>100_bases
TTCTGCAGGGACGTTACGTAAACGCAGTAGTCGTTATCGTCTCAAGCCCCATTAACCTGTCGGAGGTGCCGGGTGTCCTA
CAACACCCGGCATTAACATC

Downstream 100 bases:

>100_bases
GGGTGAGGGCCGGATGAGGATGGTGTTTGATCCGGTCCATAGTGAAGTTACAAATTACGTTGAATTGCTATGTATTTTAT
TGCCCAACGGGATAGGCGAT

Product: transcriptional regulator EutR

Products: NA

Alternate protein names: Ethanolamine operon regulatory protein [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREY
TGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPE
RVLHMLRNQSALEVKEQHKAALWGFIQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTVESISHQSYRR
LLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGF
WHLGQFATDYQQLFAEKPSLTLHQRMQELE

Sequences:

>Translated_350_residues
MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREY
TGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPE
RVLHMLRNQSALEVKEQHKAALWGFIQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTVESISHQSYRR
LLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGF
WHLGQFATDYQQLFAEKPSLTLHQRMQELE
>Mature_350_residues
MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREY
TGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPE
RVLHMLRNQSALEVKEQHKAALWGFIQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTVESISHQSYRR
LLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGF
WHLGQFATDYQQLFAEKPSLTLHQRMQELE

Specific function: Activates the transcription of the eut operon. Also positively regulates its own transcription. Probably binds ethanolamine and vitamin B12 as effectors [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=348, Percent_Identity=98.5632183908046, Blast_Score=719, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 40171; Mature: 40171

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPG
CCCCCCCCHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSAEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHCCCCHH
ATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPERVLHMLRNQSALEVKEQHKA
EECCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHH
ALWGFIQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTVESISHQSYRR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LISPWSQSMTVKDAAMQWGFWHLGQFATDYQQLFAEKPSLTLHQRMQELE
HHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPG
CCCCCCCCHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSAEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHCCCCHH
ATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPERVLHMLRNQSALEVKEQHKA
EECCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHH
ALWGFIQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTVESISHQSYRR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LISPWSQSMTVKDAAMQWGFWHLGQFATDYQQLFAEKPSLTLHQRMQELE
HHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503 [H]