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The map label for this gene is yfgB [H]

Identifier: 218548046

GI number: 218548046

Start: 658503

End: 659657

Strand: Direct

Name: yfgB [H]

Synonym: EFER_0655

Alternate gene names: 218548046

Gene position: 658503-659657 (Clockwise)

Preceding gene: 218548045

Following gene: 218548047

Centisome position: 14.35

GC content: 52.29

Gene sequence:

>1155_bases
ATGTCTGAACAATTAGTCACACCTGAAAACGTCACCACAAAAGATGGAAAAATCAACCTGCTGGATCTCAACCGTCAGCA
GATGCGGGAGTTTTTTAAAGATTTAGGTGAAAAACCCTTCCGCGCCGATCAGGTGATGAAGTGGATGTATCACTATTGCT
GCGACAACTTTGATGAGATGACCGACATCAACAAAGTGTTGCGCGGCAAGCTGAAAGAGGTGGCGGAAATTCGCGCACCG
GAAGTGGTTGAAGAACAGCGTTCATCTGACGGCACCATTAAATGGGCGATTGCCGTTGGCGATCAGCGCGTCGAAACGGT
GTATATCCCCGAAGACGACCGTGCCACGCTTTGCGTTTCCTCGCAGGTGGGGTGTGCGCTGGAGTGTAAATTCTGTTCCA
CCGCCCAGCAGGGCTTTAACCGCAATCTGCGGGTGTCGGAAATTATCGGCCAGGTGTGGCGAGCGGCGAAAATCGTTGGC
GCAGCAAAAGTCACCGGTCAGCGCCCAATCACTAACGTAGTGATGATGGGTATGGGCGAGCCGCTGCTCAACCTGAACAA
CGTCGTTCCGGCGATGGAAATCATGCTCGATGATTTCGGTTTTGGTCTGTCTAAACGTCGCGTTACGCTTTCCACTTCCG
GTGTAGTTCCGGCGCTGGATAAACTGGGCGATATGATCGACGTAGCGCTGGCAATTTCCCTGCACGCGCCGAACGACGAA
ATTCGTGACGAAATTGTGCCGATCAACAAAAAGTACAATATCGAAACGTTCCTTGCTGCGGTGCGCCGCTATCTGGAGAA
ATCTAACGCCAATCAGGGCCGAGTTACTATTGAATACGTGATGCTTGATCACGTTAACGACGGCACTGAACACGCGCACC
AACTGGCGGAGCTGCTGAAAGATACGCCGTGTAAGATCAACCTGATCCCGTGGAACCCGTTCCCTGGCGCGCCGTATGGA
CGCAGTTCGAACAGCCGTATCGACCGTTTCTCAAAAGTGCTGATGAGCTACGGTTTCACCACTATTGTGCGTAAAACTCG
TGGTGATGATATCGATGCTGCCTGTGGTCAGTTGGCGGGCGATGTTATCGACCGTACGAAACGTACTCTGCGTAAACGGA
TGCAGGGTGAGGCTATCGACGTTCGTTCTGTCTGA

Upstream 100 bases:

>100_bases
GTACAATGCAGCGCCCCCGGACGAGCAGCCGCTCACCGGGGCGTTTCTTTTTTCAACCCTCCAGGGGCCATAACGTGTAA
TAACGAGGCCGGAATAGCAT

Downstream 100 bases:

>100_bases
TAAATGTTACGCCACGGTTGTTTTATATACCGTGGCGTATTGTTTTATGAATGTAGCAGTCTGTTTCAATTGCCTGGTCA
ATAATTACGGTGCGTTTTTG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAP
EVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYG
RSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDVRSV

Sequences:

>Translated_384_residues
MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAP
EVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYG
RSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDVRSV
>Mature_383_residues
SEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPE
VVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGA
AKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDEI
RDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGR
SSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDVRSV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=384, Percent_Identity=99.21875, Blast_Score=796, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 43116; Mature: 42985

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEM
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHH
TDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVS
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEE
SQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMMGMGE
CCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
PLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHEEHHEEEEECCCHH
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLK
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHC
DTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAG
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHH
DVIDRTKRTLRKRMQGEAIDVRSV
HHHHHHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
SEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEM
CCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHH
TDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVS
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEE
SQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMMGMGE
CCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
PLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHEEHHEEEEECCCHH
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLK
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHC
DTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAG
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHH
DVIDRTKRTLRKRMQGEAIDVRSV
HHHHHHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA