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The map label for this gene is pepB [H]

Identifier: 218548040

GI number: 218548040

Start: 646488

End: 647771

Strand: Direct

Name: pepB [H]

Synonym: EFER_0649

Alternate gene names: 218548040

Gene position: 646488-647771 (Clockwise)

Preceding gene: 218548039

Following gene: 218548041

Centisome position: 14.09

GC content: 55.92

Gene sequence:

>1284_bases
ATGACAGAAGCGATGAAGATTACCCTCTCGACCCAACCTGCCGACGCGCGCTGGGGAGAAAAAGCGACTTACAGCATTAA
TAATGATGGTATTACCCTGCATTTGAACGGGGCAGACGATCTGGGGCTGATCCAGCGTGCGGCGCGCAAGATTGACGGTC
TGGGCATCAAGCATGTTCAGCTAAGCGGTGAAGGCTGGGATGCGGATCGTTGCTGGGCATTCTGGCAAGGTTACAAAGCC
CCGAAAGGCACGCGTAAAGTGGAGTGGCCGGATCTGGACGATGCCCAGCGTCAGGAACTGGATAACCGCCTGATGATCAT
CGACTGGGTGCGTGACACCATCAACGCACCAGCAGAAGAACTGGGGCCATCGCAACTGGCACAGCGTGCTGTTGATCTGA
TCAGCAACGTCGCAGGCGATCGTGTGACTTACCGGATCACCAAAGGTGAAGATCTTCGTGATCAAGGTTATATGGGGCTG
CACACTGTCGGGCGTGGTTCAGAACGTTCCCCGGTATTGCTGGCGCTGGATTACAACCCAACTGGCGATAAAGAAGCGCC
GGTGTACGCGTGCCTGGTAGGTAAAGGCATCACCTTTGATTCCGGCGGCTACAGCATCAAACAGACCGCGTTTATGGACT
CAATGAAGTCTGACATGGGCGGTGCGGCAACGGTTACCGGGGCGCTGGCATTTGCCATTACGCGCGGTCTGAACAAGCGT
GTGAAGCTGTTCCTCTGCTGTGCGGATAACCTGATTAGCGGCAACGCGTTCAAACTGGGGGATATCATTACCTATCGCAA
CGGTAAAAAAGTCGAAGTCATGAACACCGATGCGGAAGGGCGTCTGGTTCTGGCAGATGGCCTGATTGACGCCAGTGCGC
AGAAACCGGAACTGATTATCGATGCCGCGACACTCACCGGTGCGGCGAAAACCGCGCTGGGTAATGATTATCACGCGCTG
TTCAGTTTTGACGATGCGTTGGCCGGTCGCCTACTGGAGAGTGCCGCCCAGGAGAACGAACCGTTCTGGCGTCTGCCGCT
GGCGGAGTTCCACCGTAGCCAGCTGCCATCTAACTTTGCGGAACTGAACAACACCGGAAGCGCGGCGTATCCGGCAGGTG
CAAGTACGGCGGCGGGCTTCCTGTCGCACTTTGTTGAGAACTATAAACAAGGTTGGCTGCATATCGATTGTTCGGCGACT
TACCGTAAAGCACCAGTTGAACAGTGGTCTGCGGGTGCGACCGGGCTGGGTGTGCGCACGATTGCTAATCTGTTAACGGC
GTAA

Upstream 100 bases:

>100_bases
GGGCTTCACTCACTTGCCGCCTTCCTGCAACGCGAATCATTTAGCGGAAAAATCCTGGGGCTGCCAACTGGAGGCCCTTT
TACAAATAAGGATAACTAAA

Downstream 100 bases:

>100_bases
ACGTCTTATTTGGCCTACAAAATTGCGAACATCCAATAGATTGCGATTCCCTTGTAGGCCTGATAAGCGTAGCGCATCAG
GCACTTGTGCCCCGAACGTT

Product: aminopeptidase B

Products: NA

Alternate protein names: Aminopeptidase B [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKA
PKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHAL
FSFDDALAGRLLESAAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYKQGWLHIDCSAT
YRKAPVEQWSAGATGLGVRTIANLLTA

Sequences:

>Translated_427_residues
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKA
PKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHAL
FSFDDALAGRLLESAAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYKQGWLHIDCSAT
YRKAPVEQWSAGATGLGVRTIANLLTA
>Mature_426_residues
TEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAP
KGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGLH
TVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKRV
KLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALF
SFDDALAGRLLESAAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYKQGWLHIDCSATY
RKAPVEQWSAGATGLGVRTIANLLTA

Specific function: Probably plays an important role in intracellular peptide degradation [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=317, Percent_Identity=36.9085173501577, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI47155554, Length=289, Percent_Identity=35.9861591695502, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI87082123, Length=427, Percent_Identity=98.8290398126464, Blast_Score=870, Evalue=0.0,
Organism=Escherichia coli, GI1790710, Length=315, Percent_Identity=37.7777777777778, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17556903, Length=327, Percent_Identity=32.7217125382263, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17565172, Length=207, Percent_Identity=31.4009661835749, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI221379063, Length=344, Percent_Identity=31.9767441860465, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI221379062, Length=344, Percent_Identity=31.9767441860465, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21357381, Length=344, Percent_Identity=31.9767441860465, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24662227, Length=322, Percent_Identity=29.1925465838509, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21355725, Length=289, Percent_Identity=29.4117647058824, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24661038, Length=320, Percent_Identity=28.125, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161077148, Length=344, Percent_Identity=28.7790697674419, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20130057, Length=344, Percent_Identity=28.7790697674419, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20129969, Length=294, Percent_Identity=29.2517006802721, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI21355645, Length=292, Percent_Identity=27.3972602739726, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24662223, Length=292, Percent_Identity=27.3972602739726, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129963, Length=316, Percent_Identity=27.8481012658228, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922386, Length=311, Percent_Identity=27.6527331189711, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646701, Length=271, Percent_Identity=28.4132841328413, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646703, Length=271, Percent_Identity=28.4132841328413, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358201, Length=271, Percent_Identity=28.4132841328413, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008330
- InterPro:   IPR011356
- InterPro:   IPR000819 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.23 [H]

Molecular weight: Translated: 46237; Mature: 46105

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQ
CCCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
LSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEE
ECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEHHHHHCCCHHH
LGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGLHTVGRGSERSPVLLALDYNP
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCEEEEEECCC
TGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
CCCCCCCEEEEEECCCEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPELII
EEEEEEECCCCCCCCCEEECCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCEEEE
DAATLTGAAKTALGNDYHALFSFDDALAGRLLESAAQENEPFWRLPLAEFHRSQLPSNFA
EEHHHCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHH
ELNNTGSAAYPAGASTAAGFLSHFVENYKQGWLHIDCSATYRKAPVEQWSAGATGLGVRT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHCCCHHHHCCCCCCCHHHH
IANLLTA
HHHHHCC
>Mature Secondary Structure 
TEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQ
CCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
LSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEE
ECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEHHHHHCCCHHH
LGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGLHTVGRGSERSPVLLALDYNP
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCEEEEEECCC
TGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
CCCCCCCEEEEEECCCEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPELII
EEEEEEECCCCCCCCCEEECCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCEEEE
DAATLTGAAKTALGNDYHALFSFDDALAGRLLESAAQENEPFWRLPLAEFHRSQLPSNFA
EEHHHCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHH
ELNNTGSAAYPAGASTAAGFLSHFVENYKQGWLHIDCSATYRKAPVEQWSAGATGLGVRT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHCCCHHHHCCCCCCCHHHH
IANLLTA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]