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The map label for this gene is lepB [H]

Identifier: 218547904

GI number: 218547904

Start: 506255

End: 507229

Strand: Direct

Name: lepB [H]

Synonym: EFER_0506

Alternate gene names: 218547904

Gene position: 506255-507229 (Clockwise)

Preceding gene: 218547903

Following gene: 218547905

Centisome position: 11.03

GC content: 51.08

Gene sequence:

>975_bases
ATGGCGAATATGTTTGCCCTGATTCTGGTGATTGCCACACTGGTGACGGGCATTTTATGGTGCGTGGATAAATTCTTTTT
CGCACCTAAACGGCGGGAACGTCAGGCAGCGGCGCAGGCGGCTGTCGGTGACTCACTGGATAAAGCAACGCTGAAGAAAG
TTGCGCCGAAGCCTGGCTGGCTGGAAACCGGTGCTTCTGTTTTTCCGGTGCTGGCTATCGTATTGATTGTGCGTTCGTTT
ATTTATGAACCGTTCCAGATCCCGTCAGGTTCGATGATGCCGACTCTGTTAATCGGTGACTTTATTCTGGTAGAAAAGTT
TGCTTACGGCATTAAAGATCCTATCTACCAGAAAACGCTGATCGAAACCGGTCATCCGAAACGTGGCGATATTGTGGTCT
TTAAATACCCGGAAGATCCAAAGCTTGATTACATCAAGCGTGCGGTGGGTTTACCGGGCGATAAAGTCACTTACGATCCG
GTTTCAAAAGAGCTGACGATTCAGCCGGGATGCAGCTCCGGTCAGGCGTGTGAAAACGCGCTGCCGGTCACCTACTCAAA
CGTGGAACCGAGCGATTTCGTTCAGACCTTCTCACGTCGTAATGGTGGGGAAGCAACCAGCGGATTCTTTGAAGTGCCGA
AAAACGAAACCAAAGAAAATGGAATTCGTCTTTCCGAGCGTAAAGAGACTCTGGGTGATGTGACGCACCGTATTTTGACA
GTGCCGATTGCCCAGGATCAGGTAGGGATGTATTACCAGCAGCCAGGGCAACAACTGGCAACCTGGATTGTTCCACCGGG
CCAATACTTCATGATGGGTGACAACCGTGACAACAGCGCGGACAGCCGTTACTGGGGCTTTGTGCCGGAAGCGAATCTGG
TAGGTCGGGCAACGGCTATCTGGATGAGTTTCGATAAGCAAGAAGGCGAATGGCCGACTGGTGTGCGCTTAAGTCGTATT
GGCGGCATCCATTAA

Upstream 100 bases:

>100_bases
GAAACGCATGAAGCAGATCGGTAACGTCGAGCTGCCGCAGGAAGCGTTCCTCGCCATTCTGCACGTCGGCAAAGACAACA
AATAACCCTTAGGAGTTGGC

Downstream 100 bases:

>100_bases
TAGCTTATCTTCGTTCACGTTGTCGCCGTTATGGCGACAACGTGAATTATTTATGAGATAAATCTCCCGTGGCTAACGAC
ATCCCCCGTCGTTGTGTATA

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAVGDSLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSF
IYEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETKENGIRLSERKETLGDVTHRILT
VPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRI
GGIH

Sequences:

>Translated_324_residues
MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAVGDSLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSF
IYEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETKENGIRLSERKETLGDVTHRILT
VPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRI
GGIH
>Mature_323_residues
ANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAVGDSLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFI
YEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDPV
SKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETKENGIRLSERKETLGDVTHRILTV
PIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIG
GIH

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=324, Percent_Identity=99.3827160493827, Blast_Score=667, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR019766 [H]

Pfam domain/function: PF00717 Peptidase_S24; PF10502 Peptidase_S26 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 35975; Mature: 35844

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAVGDSLDKATLKKVAPKPGW
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
LETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTL
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
IETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDPVSKELTIQPGCSSGQACENA
HHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEECCCCCEEEECCCCCCCCHHHCC
LPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETKENGIRLSERKETLGDVTHRILT
CCEEECCCCHHHHHHHHHHCCCCCCCCCCEECCCCCCHHCCCCCHHHHHHHHHHHHHHEE
VPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGRATAI
EECCCCHHHHHHHCCCHHEEEEEECCCCEEEEECCCCCCCCCCEECCCCCCCHHCCCEEE
WMSFDKQEGEWPTGVRLSRIGGIH
EEEECCCCCCCCCCEEHHCCCCCC
>Mature Secondary Structure 
ANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAVGDSLDKATLKKVAPKPGW
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
LETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTL
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
IETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDPVSKELTIQPGCSSGQACENA
HHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEECCCCCEEEECCCCCCCCHHHCC
LPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETKENGIRLSERKETLGDVTHRILT
CCEEECCCCHHHHHHHHHHCCCCCCCCCCEECCCCCCHHCCCCCHHHHHHHHHHHHHHEE
VPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGRATAI
EECCCCHHHHHHHCCCHHEEEEEECCCCEEEEECCCCCCCCCCEECCCCCCCHHCCCEEE
WMSFDKQEGEWPTGVRLSRIGGIH
EEEECCCCCCCCCCEEHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6311837; 9278503; 2202591; 1618816; 1546969; 8394311; 2551889; 9823901 [H]