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The map label for this gene is ppnK [H]

Identifier: 218547867

GI number: 218547867

Start: 463631

End: 465259

Strand: Reverse

Name: ppnK [H]

Synonym: EFER_0458

Alternate gene names: 218547867

Gene position: 465259-463631 (Counterclockwise)

Preceding gene: 218547869

Following gene: 218547866

Centisome position: 10.14

GC content: 49.23

Gene sequence:

>1629_bases
ATGCTGGGCCGTGATTTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTAC
GACCGTTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATG
GCCTGATGCACGTTCGGGTCCAGCGGCACGTTAGTTTCGGCGATCACTTCAACGCCAAACTTACGCACAACATCCAGCAT
CGACTTCAGCGTCAGCTCAATACCTTCAACCATCGCGGACATATCCGGGTTAGCTTTATCAGCCACTTCCAGCGCACGAT
CCAGGCTATCAATCACCGGCAGCAATTCGTTGATGAATTTCTCCAGCGCGAATTTATGTGCTTTTTCGATATCCAGTTCA
GTACGGCGACGCAGGTTTTCCATTTCGGCTTTTACGCGCAAAATGCCGTCACGTTCACGGATCTGGGCTTCAGCCAGCTG
AGCTTCGAGATTCGCAATTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTT
CGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCG
TTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTC
ATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGACACCCACGGCACCC
TACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCG
CTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTGGCTGATCTCGCGGTAGTCGTT
GGTGGCGACGGTAATATGCTGGGCGCGGCGCGCACACTCGCCCGTTACGATATTAAAGTTATTGGAATCAACCGTGGCAA
CCTGGGTTTCCTGACCGACCTTGACCCCGATAACGCTCAGCAACAATTAGCCGATGTGCTGGAAGGTCACTACATCAGCG
AGAAACGTTTTTTGCTGGAAGCGCAAGTCTGTCAGCAAGATTGCCAAAAGCGCATCAGCACCGCGATAAATGAAGTGGTG
CTTCACCCAGGAAAAGTGGCGCATATGATTGAATTCGAAGTATATATCGACGAGATATTTGCGTTTTCGCAGCGTTCCGA
TGGCCTGATTATTTCGACACCTACAGGCTCTACTGCCTATTCTCTCTCGGCAGGCGGTCCAATCCTGACGCCTTCTCTGG
ATGCAATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGT
CTGCGTTTTTCACATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGAGAAGA
TGTCCTGATTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGCTATTTCAATACATTAAGCACCA
AGCTCGGCTGGTCAAAAAAATTATTCTAA

Upstream 100 bases:

>100_bases
TCTCGATGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGTGAGAGTGTGGCGGAGTAAC
GAAAACGGCCCGGCATTCGC

Downstream 100 bases:

>100_bases
TTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAA
CAACTATGTTGGCACAACTG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MLGRDLLRKQKLSFCFRYSNHRRTNRTTVQRITLLHNAQYVTWRNVIRFHHCDGLMHVRVQRHVSFGDHFNAKLTHNIQH
RLQRQLNTFNHRGHIRVSFISHFQRTIQAINHRQQFVDEFLQREFMCFFDIQFSTATQVFHFGFYAQNAVTFTDLGFSQL
SFEIRNFFIARIHLLSRSFWLNCLNLFVLIHDNFFRGLPLRRFLFFTTHEFLRVFFRIHLANFAYYGDQFQGFKGSTHIV
INLRNKDLGKMNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIAHELQLKNVKTGTLAEIGQLADLAVVV
GGDGNMLGAARTLARYDIKVIGINRGNLGFLTDLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVV
LHPGKVAHMIEFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSARPLVINSSSTIR
LRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYSYFNTLSTKLGWSKKLF

Sequences:

>Translated_542_residues
MLGRDLLRKQKLSFCFRYSNHRRTNRTTVQRITLLHNAQYVTWRNVIRFHHCDGLMHVRVQRHVSFGDHFNAKLTHNIQH
RLQRQLNTFNHRGHIRVSFISHFQRTIQAINHRQQFVDEFLQREFMCFFDIQFSTATQVFHFGFYAQNAVTFTDLGFSQL
SFEIRNFFIARIHLLSRSFWLNCLNLFVLIHDNFFRGLPLRRFLFFTTHEFLRVFFRIHLANFAYYGDQFQGFKGSTHIV
INLRNKDLGKMNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIAHELQLKNVKTGTLAEIGQLADLAVVV
GGDGNMLGAARTLARYDIKVIGINRGNLGFLTDLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVV
LHPGKVAHMIEFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSARPLVINSSSTIR
LRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYSYFNTLSTKLGWSKKLF
>Mature_542_residues
MLGRDLLRKQKLSFCFRYSNHRRTNRTTVQRITLLHNAQYVTWRNVIRFHHCDGLMHVRVQRHVSFGDHFNAKLTHNIQH
RLQRQLNTFNHRGHIRVSFISHFQRTIQAINHRQQFVDEFLQREFMCFFDIQFSTATQVFHFGFYAQNAVTFTDLGFSQL
SFEIRNFFIARIHLLSRSFWLNCLNLFVLIHDNFFRGLPLRRFLFFTTHEFLRVFFRIHLANFAYYGDQFQGFKGSTHIV
INLRNKDLGKMNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIAHELQLKNVKTGTLAEIGQLADLAVVV
GGDGNMLGAARTLARYDIKVIGINRGNLGFLTDLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVV
LHPGKVAHMIEFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSARPLVINSSSTIR
LRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYSYFNTLSTKLGWSKKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=259, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788968, Length=292, Percent_Identity=100, Blast_Score=610, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6320794, Length=243, Percent_Identity=28.8065843621399, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6325068, Length=223, Percent_Identity=32.7354260089686, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=29.1666666666667, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28573830, Length=297, Percent_Identity=29.6296296296296, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28573832, Length=297, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28573826, Length=297, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28573828, Length=297, Percent_Identity=29.6296296296296, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI161077047, Length=297, Percent_Identity=29.6296296296296, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653422, Length=270, Percent_Identity=27.037037037037, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI281363323, Length=270, Percent_Identity=27.037037037037, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653424, Length=270, Percent_Identity=27.037037037037, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281363321, Length=270, Percent_Identity=27.037037037037, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129957, Length=270, Percent_Identity=27.037037037037, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 62870; Mature: 62870

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRDLLRKQKLSFCFRYSNHRRTNRTTVQRITLLHNAQYVTWRNVIRFHHCDGLMHVRV
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHCCCCCEEEEEE
QRHVSFGDHFNAKLTHNIQHRLQRQLNTFNHRGHIRVSFISHFQRTIQAINHRQQFVDEF
EECCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
LQREFMCFFDIQFSTATQVFHFGFYAQNAVTFTDLGFSQLSFEIRNFFIARIHLLSRSFW
HHCCEEEEEEEEECHHHHHHEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LNCLNLFVLIHDNFFRGLPLRRFLFFTTHEFLRVFFRIHLANFAYYGDQFQGFKGSTHIV
HHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEE
INLRNKDLGKMNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIAHELQLK
EEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
NVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLTDLDPDNAQ
CCCCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHCEEEEEEECCCCEEEEECCCCCCHH
QQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMIEFEVYIDEIF
HHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHH
AFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSARPLVINSSSTIR
HHCCCCCCEEEECCCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCCEEEEECCCEEE
LRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYSYFNTLSTKLGWSKK
EEEECCCCCEEEECCCEEEEEECCCCCEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCC
LF
CC
>Mature Secondary Structure
MLGRDLLRKQKLSFCFRYSNHRRTNRTTVQRITLLHNAQYVTWRNVIRFHHCDGLMHVRV
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHCCCCCEEEEEE
QRHVSFGDHFNAKLTHNIQHRLQRQLNTFNHRGHIRVSFISHFQRTIQAINHRQQFVDEF
EECCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
LQREFMCFFDIQFSTATQVFHFGFYAQNAVTFTDLGFSQLSFEIRNFFIARIHLLSRSFW
HHCCEEEEEEEEECHHHHHHEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LNCLNLFVLIHDNFFRGLPLRRFLFFTTHEFLRVFFRIHLANFAYYGDQFQGFKGSTHIV
HHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEE
INLRNKDLGKMNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIAHELQLK
EEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
NVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLTDLDPDNAQ
CCCCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHCEEEEEEECCCCEEEEECCCCCCHH
QQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMIEFEVYIDEIF
HHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHH
AFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSARPLVINSSSTIR
HHCCCCCCEEEECCCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCCEEEEECCCEEE
LRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYSYFNTLSTKLGWSKK
EEEECCCCCEEEECCCEEEEEECCCCCEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCC
LF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA