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The map label for this gene is ygaF [H]

Identifier: 218547825

GI number: 218547825

Start: 423066

End: 424334

Strand: Reverse

Name: ygaF [H]

Synonym: EFER_0412

Alternate gene names: 218547825

Gene position: 424334-423066 (Counterclockwise)

Preceding gene: 218547826

Following gene: 218547824

Centisome position: 9.25

GC content: 56.74

Gene sequence:

>1269_bases
ATGTATGATTTTGTGATTATTGGCGGCGGCATCATTGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGATGC
ACGCATTGCGTTGCTGGAAAAAGAGTCCGGCCCTGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGG
TCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACTAAAGCCTTTTGCGATCAAAAC
GGCATTCGCTACGACAACTGCGGCAAGATGCTGGTCGCCACGTCTGAACTCGAAATGGAACGGATGCGCGCTTTATGGGA
ACGCACAGCGGCGAACGGTATTGAACGCGAGTGGTTAAACGCCGACGAACTGCGCGAGTGCGAACCGAATATCACCGGGC
TCGGCGGCATTTTTGTGCCGTCCAGCGGTATTGTCAGCTACCGCGAAGTGACGGCGGCGATGGCAAAAATCTTCCAGGCC
AGAGGCGGTGAGATTATTTATAACGCCGAAGTCAGCGGCCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGCCA
GGGCGGCGAATATGAAGCATCGACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGCCTCG
AACCTGGCTTTATTATCTGCCCGTTCCGCGGCGAGTATTTCCGCCTTGCGCCGGAGCATAACCACATTGTTAACCACCTG
ATTTACCCCATCCCCGACCCGGCAATGCCGTTTTTGGGCGTGCATCTCACTCGTATGATCGACGGCAGCGTGACCGTTGG
GCCAAACGCGGTGCTGGCTTTTAAACGTGAAGGCTATCGCAAGCGCGACTTCTCATTTAGCGACACGCTGGAGATTTTGG
GCTCGTCGGGGATTCGCCGGGTGCTGCAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGC
GGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCGGGCTTTCGTTAAGCGATCTTCAGCCCTGGCCCGCCGGTGTGCGGGC
GCAGGCAGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATG
CGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAG
AGTAACCCCGGACGCACGCTGCGAGCGGCACGTAGTGTGGATGCCTTACACGCCGCCTTTAATCAATAA

Upstream 100 bases:

>100_bases
CATCCGGATCTGCGTCGTGAACTGATGCGCCAGCGCGGCTATTTCGCTTACGCCACTCACCACTACCAGACGCATCAGTA
AGCGCAAAGGAATTGAGCGG

Downstream 100 bases:

>100_bases
CCTTTGAAAACAGGATGTAGCGATGAAACTGAACGACAGTAACTTATTCCGCCAGCAGGCGTTAATTAACGGGGAATGGC
TGGACGCCAACAATGGCGAA

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQN
GIRYDNCGKMLVATSELEMERMRALWERTAANGIEREWLNADELRECEPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
RGGEIIYNAEVSGLNEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNHIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKS
GYLRLVQKYCPGLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQ
SNPGRTLRAARSVDALHAAFNQ

Sequences:

>Translated_422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQN
GIRYDNCGKMLVATSELEMERMRALWERTAANGIEREWLNADELRECEPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
RGGEIIYNAEVSGLNEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNHIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKS
GYLRLVQKYCPGLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQ
SNPGRTLRAARSVDALHAAFNQ
>Mature_422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQN
GIRYDNCGKMLVATSELEMERMRALWERTAANGIEREWLNADELRECEPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
RGGEIIYNAEVSGLNEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNHIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKS
GYLRLVQKYCPGLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQ
SNPGRTLRAARSVDALHAAFNQ

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=398, Percent_Identity=41.4572864321608, Blast_Score=320, Evalue=1e-87,
Organism=Escherichia coli, GI87082148, Length=422, Percent_Identity=98.3412322274881, Blast_Score=861, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17565764, Length=401, Percent_Identity=42.8927680798005, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24585081, Length=407, Percent_Identity=39.8034398034398, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 45937; Mature: 45937

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGS
CCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCCCCEEEEEEEECCCC
LKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERMRALWERTAANGIEREWLN
CEEEEEEECCCHHHHHHCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHCC
ADELRECEPNITGLGGIFVPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSGLNEHKNG
HHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
VVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNHIVNHL
EEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHE
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRR
EECCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHH
VLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPGLSLSDLQPWPAGVRAQAVSPDGKLIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEECCE
LFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAF
EEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
NQ
CC
>Mature Secondary Structure
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGS
CCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCCCCEEEEEEEECCCC
LKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERMRALWERTAANGIEREWLN
CEEEEEEECCCHHHHHHCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHCC
ADELRECEPNITGLGGIFVPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSGLNEHKNG
HHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
VVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNHIVNHL
EEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHE
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRR
EECCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHH
VLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPGLSLSDLQPWPAGVRAQAVSPDGKLIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEECCE
LFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAF
EEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
NQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]