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The map label for this gene is nrdE [H]

Identifier: 218547808

GI number: 218547808

Start: 408979

End: 411123

Strand: Reverse

Name: nrdE [H]

Synonym: EFER_0395

Alternate gene names: 218547808

Gene position: 411123-408979 (Counterclockwise)

Preceding gene: 218547809

Following gene: 218547807

Centisome position: 8.96

GC content: 52.03

Gene sequence:

>2145_bases
TTGGCAACGACAACCGCAGAACGCCTGACGCAGGAAACAATGGATTACCACGCCCTGAATGCGATGCTTAACCTTTACGA
TAGCGCAGGTCGCATTCAGTTCGATAAAGATCGCCAGGCCGTTGACGCCTTTATGGCGACACATGTGCGTCCGAACAGTG
TGACCTTCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAACGTCCTCAACCGCTAC
TCTCGCGATTTTGTCATTACGCTGTTTGCCCACGCACACGCCAGCGGTTTTCGTTTTCAGACATTCCTCGGAGCATGGAA
GTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAGCGTTATCTGGAAGATTTTGCCGATCGAGTCACGATGGTGG
CGCTGACGCTGGCACAAGGCGATAAAACACTGGCTAAGCAACTGACCGATGAGATGCTGTCAGGACGCTTTCAGCCTGCA
ACACCGACATTCCTCAACTGCGGTAAGCGGCAGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATAT
GGAGTCGATTGGCCGGGCAGTAAATTCCGCACTACAACTGTCGAAACGCGGCGGCGGCGTGGCATTTTTGCTGTCGAATC
TGCGGGAAGCGGGCGCGCCAATTAAACGTATTGAAAATCAATCTTCTGGCGTGATTCCGGTGATGAAAATGCTGGAGGAC
GCATTTTCCTATGCCAACCAGCTCGGCGCTCGTCAGGGTGCTGGCGCAGTCTATTTACATGCCCATCATCCCGATATTCT
GCGTTTTCTCGATACGAAACGGGAAAATGCCGACGAGAAAATCCGCATTAAAACACTGTCGCTGGGGGTGGTGATCCCGG
ATATCACCTTCCATCTGGCAAAAGAGAATGCGCAGATGGCGCTGTTTTCGCCCTATGACGTTGAGCGAGTTTATGGCAAG
CCGTTTGCCGATATCGCCATCAGCGAACACTATGCCGAACTGGTAGCCGATGAACGCATCCGCAAAAAATACATCAACGC
CCGTGATTTCTTCCAGCGGCTGGCGGAGATCCAGTTTGAATCCGGCTATCCCTACATCATGTATGAAGACACGGTTAACC
GTGCTAACCCTATTGCCGGGCGTATAAATATGAGCAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTAT
GACGAGAATCTCGACTATGCCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCCCACACCATGGA
TTCCCCCGATTTTGCCCGCACGGTAGAGATCGCCGTGCGCGGTTTAACGGCAGTATCAGATATAAGTCATATCCGCAGCG
TGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGTTATCTGGCGCGA
GAAGGCATCGCTTATGGATCGCCGGAAGCACTCGATTTCACCAATCTCTATTTCTATACCATCACCTGGCACGCGCTGCG
TACCTCGATGTTACTGGCGCGCGAACGCGGCGAAACCTTTGCCGGGTTCAAACAGTCGCGCTATGCCAGCGGTGAATATT
TTAGCCAATATCTGCAAGGTAACTGGCAGCCGAAAACGGCGAAAGTTGGCGAGTTGTTTGCTCGTAGCAGTATCACGTTA
CCTACCCGCGAAATGTGGGCGCAACTGCGCGATGACGTAATGCGCTATGGCATTTACAACCAGAATCTCCAGGCAGTGCC
GCCAACTGGCTCCATCTCTTATATCAACCATGCCACGTCGAGCATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAG
AAGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAA
ATCGGCGCAGAAAAGATCATCGACACCTACGCCGAAGCGGCTCGCCATGTCGATCAAGGGCTGTCGCTGACGCTGTTTTT
CCCTGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTATGCCTGGCGTAAAGGTATCAAAACGCTCTACTACA
TCCGCCTGCGTCAGATGGCGCTGGAAGGTACTGAAATTGAAGGCTGCGTCTCCTGCGCGCTTTAA

Upstream 100 bases:

>100_bases
ATGTGATTGCCCGGAAATGCGGCGTGCCGTGGCTGTACCGCTTTGAACTCATGGGTACGCAAAGCGATATCGAAAACGTT
CGTAAAGGAGTAACCGAATT

Downstream 100 bases:

>100_bases
GGAATATCTATGAAACTCTCACGTATCAGCGCCATCAACTGGAATAAGATATCTGACGATAAAGATCTGGAGGTGTGGAA
CCGCCTGACCAGCAATTTCT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: R1E protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQQRLNWLVNEGYYDENVLNRY
SRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDKTLAKQLTDEMLSGRFQPA
TPTFLNCGKRQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
PFADIAISEHYAELVADERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEY
DENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVEIAVRGLTAVSDISHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSSITL
PTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYE
IGAEKIIDTYAEAARHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL

Sequences:

>Translated_714_residues
MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQQRLNWLVNEGYYDENVLNRY
SRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDKTLAKQLTDEMLSGRFQPA
TPTFLNCGKRQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
PFADIAISEHYAELVADERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEY
DENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVEIAVRGLTAVSDISHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSSITL
PTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYE
IGAEKIIDTYAEAARHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
>Mature_713_residues
ATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQQRLNWLVNEGYYDENVLNRYS
RDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDKTLAKQLTDEMLSGRFQPAT
PTFLNCGKRQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDA
FSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKP
FADIAISEHYAELVADERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYD
ENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVEIAVRGLTAVSDISHIRSVPSIEAGNAASHAIGLGQMNLHGYLARE
GIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSSITLP
TREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEI
GAEKIIDTYAEAARHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reductio

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=600, Percent_Identity=28.1666666666667, Blast_Score=191, Evalue=2e-48,
Organism=Escherichia coli, GI1789030, Length=714, Percent_Identity=97.3389355742297, Blast_Score=1456, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=582, Percent_Identity=25.9450171821306, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17555242, Length=747, Percent_Identity=25.1673360107095, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320914, Length=619, Percent_Identity=26.1712439418417, Blast_Score=173, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6322123, Length=643, Percent_Identity=26.4385692068429, Blast_Score=170, Evalue=8e-43,
Organism=Drosophila melanogaster, GI17352481, Length=672, Percent_Identity=24.5535714285714, Blast_Score=153, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80495; Mature: 80364

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCEECCCHH
QRLNWLVNEGYYDENVLNRYSRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGK
HHHHHHHCCCCCCHHHHHHHCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHEEEEEECCCH
RYLEDFADRVTMVALTLAQGDKTLAKQLTDEMLSGRFQPATPTFLNCGKRQRGELVSCFL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
HHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KENAQMALFSPYDVERVYGKPFADIAISEHYAELVADERIRKKYINARDFFQRLAEIQFE
CCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEC
SGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCHHHCCCCEEECCCCE
NIAHTMDSPDFARTVEIAVRGLTAVSDISHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCCCCHHHHH
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQG
CCCCCCCCCCEEECEEEEEEEEHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHCC
NWQPKTAKVGELFARSSITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCC
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEAARHVDQG
CCCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure 
ATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCEECCCHH
QRLNWLVNEGYYDENVLNRYSRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGK
HHHHHHHCCCCCCHHHHHHHCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHEEEEEECCCH
RYLEDFADRVTMVALTLAQGDKTLAKQLTDEMLSGRFQPATPTFLNCGKRQRGELVSCFL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
HHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KENAQMALFSPYDVERVYGKPFADIAISEHYAELVADERIRKKYINARDFFQRLAEIQFE
CCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEC
SGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCHHHCCCCEEECCCCE
NIAHTMDSPDFARTVEIAVRGLTAVSDISHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCCCCHHHHH
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQG
CCCCCCCCCCEEECEEEEEEEEHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHCC
NWQPKTAKVGELFARSSITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCC
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEAARHVDQG
CCCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8820648; 2158980 [H]