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The map label for this gene is alaS [H]

Identifier: 218547790

GI number: 218547790

Start: 388390

End: 391020

Strand: Direct

Name: alaS [H]

Synonym: EFER_0377

Alternate gene names: 218547790

Gene position: 388390-391020 (Clockwise)

Preceding gene: 218547789

Following gene: 218547791

Centisome position: 8.46

GC content: 53.48

Gene sequence:

>2631_bases
ATGAGCAAGAGCACCGCTGAGATCCGTCAGGCGTTTCTCGACTTTTTCCATAGTAAGGGACATCAGGTAGTTGCCAGCAG
CTCCCTGGTACCCCATAACGACCCAACTTTGTTGTTTACCAACGCCGGGATGAACCAGTTCAAGGATGTGTTCCTTGGGC
TCGACAAGCGTAATTATTCCCGCGCTACCACTTCCCAACGCTGCGTGCGTGCGGGTGGTAAACACAACGACCTGGAAAAC
GTCGGTTACACCGCGCGTCACCATACCTTCTTCGAAATGCTGGGCAACTTCAGCTTCGGCGACTATTTCAAACACGATGC
TATTCAGTTTGCATGGGAACTGCTGACCAGCGAAAAATGGTTTGCCCTGCCGAAAGAGCGTCTGTGGGTTACCGTCTATG
AAAGCGACGACGAAGCCTACGAAATCTGGGAAAAAGAAGTAGGGATCCCGCGCGAACGTATTATTCGCATCGGCGATAAC
AAAGGTGCGCCATACGCATCTGACAACTTCTGGCAGATGGGCGACACTGGTCCGTGTGGCCCGTGCACCGAAATCTTCTA
CGATCACGGCGACCACATTTGGGGTGGCCCTCCGGGAAGTCCGGAAGAAGACGGCGACCGTTACATTGAGATTTGGAACA
TCGTCTTCATGCAGTTCAACCGCCAGGCAGATGGCACGATGGAACCGCTGCCGAAGCCGTCTGTAGATACCGGTATGGGT
CTGGAGCGTATTGCTGCGGTGCTGCAACACGTTAACTCTAACTATGACATTGACCTGTTCCGCACGCTGATCCAGGCAGT
AGCAAAAGTCACTGGCGCGACCGATCTGAGCAATAAATCGCTGCGCGTAATCGCTGACCACATTCGTTCTTGTGCGTTCC
TGATCGCGGATGGCGTAATGCCGTCCAACGAAAACCGTGGTTATGTACTGCGTCGTATCATTCGTCGCGCAGTGCGTCAC
GGTAATATGCTCGGCGCGAAAGAAACCTTCTTCTACAAACTGGTTGGTCCGCTGATCGACGTTATGGGCTCGGCGGGTGA
AGACCTGAAACGCCAGCAGGCGCAGGTTGAGCAGGTGCTGAAGACTGAAGAAGAGCAGTTTGCCCGCACTCTGGAGCGCG
GTCTGGCGCTGCTGGACGAAGAGCTGGCGAAACTTTCTGGCGATACGCTGGATGGCGAAACTGCTTTCCGTCTGTATGAC
ACCTACGGTTTCCCGGTTGACCTGACGGCTGATGTTTGTCGTGAACGCAACATCAAAGTTGACGAAGCTGGTTTTGAAGC
AGCAATGGAAGAGCAGCGTCGTCGTGCGCGCGAAGCCAGCGGCTTTGGTGCCGATTACAACGCAATGATCCGTGTTGACA
GCGCATCTGAGTTTAAAGGTTATGACCATCTGGAACTGAACGGCAAAGTGACCGCGCTGTTTGTTGATGGTAAAGCGGTT
GATGCCATTAATGCAGGCCAGGAAGCTGTGGTCGTGCTGGATCAAACGCCATTCTATGCGGAATCTGGCGGTCAGGTTGG
CGATAAAGGCGAACTGAAAGGTGCTAACTTCTCCTTTGCGGTGGAAGATACGCAGAAATACGGCCAGGCGATTGGTCATA
TCGGTAAACTTGCTGCGGGTTCTCTGAAAGTGGGCGACGCGGTGCAGGCTGATGTTGATGAGGCTCGTCGCGCTCGTATT
CGTCTGAATCACTCCGCAACGCACCTGATGCACGCTGCGCTGCGCAAGGTTCTGGGTACTCATGTATCGCAGAAAGGTTC
ACTGGTTAACGACAAAGTGCTGCGCTTCGACTTTTCACACAACGAAGCGATGAAACCAGAAGAGATTCGAGCAGTCGAAG
ACCTGGTGAACGCGCAGATTCGCCGTAACTTGCCGATCGAAACCAACATCATGGATCTCGAAGCGGCGAAAGCGAAAGGT
GCGATGGCGCTGTTTGGCGAGAAGTATGATGAGCGCGTACGCGTGCTGAGCATGGGCGATTTCTCCACCGAGCTGTGTGG
CGGTACTCACGCCAGCCGCACCGGTGATATTGGTCTGTTCCGCATCATCTCTGAATCGGGTACTGCTGCAGGCGTTCGTC
GTATCGAAGCGGTAACCGGAGAAGGCGCTATCGCCACCGTTCATGCAGACAGCGATCGCTTAAGCGAAGTCGCACATCTG
CTGAAAGGCGATAGCAATAATCTGGCTGATAAAGTGCGTTCAGTACTGGAACGTACGCGTCAGCTGGAAAAAGAGTTACA
ACAGCTTAAAGAACAGGCTGCCGCACAGGAGAGTGCAAATCTTTCCAGTAAGGCAATTGATGTTAATGGTGTTAAGCTGT
TGGTTAGCGAGCTTAGCGGTGTTGAGCCGAAGATGTTGCGTACCATGGTTGACGATTTAAAAAATCAGCTGGGGTCGACA
ATTATCGTGCTGGCAACGGTAGCCGAAGGTAAGGTTTCTCTGATTGCAGGCGTATCTAAGGACGTCACAGATCGTGTGAA
AGCAGGGGAACTGATTGGTATGGTCGCTCAGCAGGTGGGTGGCAAGGGTGGTGGACGTCCTGACATGGCGCAAGCCGGTG
GTACGGATGCTGCGGCCTTACCTGCAGCGTTAGCCAGTGTGAAAGGCTGGGTCAGCGCGAAATTGCAATAA

Upstream 100 bases:

>100_bases
AAGAAAACTTATCTTATTCCCACTTTTCAGTTACCAGCCCGGCGGTTAAGACACGCTGTAGCTGGTGGCGATATTTCGTT
AGCTTGATTTCAGGATAATT

Downstream 100 bases:

>100_bases
TATAAGCGTCAGGCAATGCCGTGGACTCGCTTCACGGCATTCGCATTAACGCTATCGACAACGATAAAGTCAGGTTGAAG
TTGTGTATATCGGCTAAACT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 876; Mature: 875

Protein sequence:

>876_residues
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
LERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRH
GNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYD
TYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAV
DAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARI
RLNHSATHLMHAALRKVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKG
AMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHL
LKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLGST
IIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ

Sequences:

>Translated_876_residues
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
LERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRH
GNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYD
TYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAV
DAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARI
RLNHSATHLMHAALRKVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKG
AMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHL
LKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLGST
IIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ
>Mature_875_residues
SKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENV
GYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNK
GAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGL
ERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHG
NMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDT
YGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVD
AINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIR
LNHSATHLMHAALRKVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGA
MALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLL
KGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLGSTI
IVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=979, Percent_Identity=38.6108273748723, Blast_Score=567, Evalue=1e-161,
Organism=Homo sapiens, GI38569417, Length=965, Percent_Identity=35.2331606217617, Blast_Score=482, Evalue=1e-136,
Organism=Escherichia coli, GI1789048, Length=876, Percent_Identity=99.6575342465753, Blast_Score=1800, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=980, Percent_Identity=37.4489795918367, Blast_Score=554, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI17536681, Length=743, Percent_Identity=33.7819650067295, Blast_Score=385, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324911, Length=776, Percent_Identity=42.7835051546392, Blast_Score=582, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24582809, Length=956, Percent_Identity=37.8661087866109, Blast_Score=572, Evalue=1e-163,
Organism=Drosophila melanogaster, GI45552267, Length=956, Percent_Identity=37.8661087866109, Blast_Score=572, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24658214, Length=806, Percent_Identity=32.0099255583127, Blast_Score=359, Evalue=5e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 95975; Mature: 95844

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYS
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKW
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
FALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCG
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCC
PCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKV
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCC
DEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAV
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCEEEECCEEEEEEECCCEE
DAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYGQAIGHIGKLAAG
CCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCC
SLKVGDAVQADVDEARRARIRLNHSATHLMHAALRKVLGTHVSQKGSLVNDKVLRFDFSH
CCCCCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
NEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHCCCHHHHHCCHHCCEEEEEECC
FSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHL
CCHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
LKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSG
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHCC
VEPKMLRTMVDDLKNQLGSTIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVG
CCHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHC
GKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYS
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKW
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
FALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCG
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCC
PCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKV
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCC
DEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAV
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCEEEECCEEEEEEECCCEE
DAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYGQAIGHIGKLAAG
CCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCC
SLKVGDAVQADVDEARRARIRLNHSATHLMHAALRKVLGTHVSQKGSLVNDKVLRFDFSH
CCCCCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
NEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHCCCHHHHHCCHHCCEEEEEECC
FSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHL
CCHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
LKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSG
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHCC
VEPKMLRTMVDDLKNQLGSTIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVG
CCHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHC
GKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA