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The map label for this gene is hycH [H]

Identifier: 218547774

GI number: 218547774

Start: 370624

End: 371034

Strand: Direct

Name: hycH [H]

Synonym: EFER_0359

Alternate gene names: 218547774

Gene position: 370624-371034 (Clockwise)

Preceding gene: 218547773

Following gene: 218547775

Centisome position: 8.08

GC content: 51.82

Gene sequence:

>411_bases
ATGAGTGAAAAGGTGGTGTTCAGTCAACTGAGCCGTAAATTTATTGATGAGAACGATGCCACGCCCGCCGAGGCGCAGCA
GGTGGTCTATTATAGCCTGGCGATTGGTCACCACCTTGGGGTTATCGATTGCCTGGAAGCCGCGCTCACCTGCCCGTGGG
ATGAATATCTGGCATGGATTGCCACTCTGGAGGCGGGCAGTGAAGCCCGCCGTAAAATGGAAGGCGTGCCGAAATATGGT
GAGATCGTTATCGACATTAACCATGTGCCGATGCTGGCCAACGCATTCGATAAAGCCCGGTCAGCGCAAACTTCGCAGCA
GCAGGAATGGAGTACAATGCTGTTAAGTATGCTGCATGATATTCATCAGGAAAACGCCATCTATTTGATGGTGAGGAGAT
TGCGTGACTGA

Upstream 100 bases:

>100_bases
GTGAAGAGCAGGTTGCACGCTGGCTGGAAGCAGAAAACGATCCGCGTCTGAACGAGATTGTCAGCCATCTGAATCATGTT
GTTGAAGAGGCGCGTATCCG

Downstream 100 bases:

>100_bases
CGTTTTACTCTGTGTTGGCAATAGCATGATGGGCGATGATGGCGCGGGCCCGTTGCTGGCGGAAAAGTGCGCCGCCGCGC
CGAAAGGCAACTGGGTGGTG

Product: hydrogenase-3 maturation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYG
EIVIDINHVPMLANAFDKARSAQTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD

Sequences:

>Translated_136_residues
MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYG
EIVIDINHVPMLANAFDKARSAQTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD
>Mature_135_residues
SEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGE
IVIDINHVPMLANAFDKARSAQTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD

Specific function: Seems to be required for the conversion of a precursor form of the large subunit of hydrogenlyase (hycE) into a mature form [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli hyfJ [H]

Homologues:

Organism=Escherichia coli, GI1789073, Length=136, Percent_Identity=99.2647058823529, Blast_Score=284, Evalue=1e-78,
Organism=Escherichia coli, GI87082116, Length=131, Percent_Identity=48.0916030534351, Blast_Score=123, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010005
- ProDom:   PD021295 [H]

Pfam domain/function: PF07450 HycH [H]

EC number: NA

Molecular weight: Translated: 15481; Mature: 15350

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWI
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
ATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARSAQTSQQQEWSTMLLSMLHD
HHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IHQENAIYLMVRRLRD
HHHCCHHHHHHHHHCC
>Mature Secondary Structure 
SEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWI
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
ATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARSAQTSQQQEWSTMLLSMLHD
HHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IHQENAIYLMVRRLRD
HHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]