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The map label for this gene is fhlA [H]

Identifier: 218547761

GI number: 218547761

Start: 357548

End: 359638

Strand: Reverse

Name: fhlA [H]

Synonym: EFER_0346

Alternate gene names: 218547761

Gene position: 359638-357548 (Counterclockwise)

Preceding gene: 218547762

Following gene: 218547760

Centisome position: 7.84

GC content: 50.6

Gene sequence:

>2091_bases
ATGTCGTATACACCAATGAGCGATCTCGGACAACAAGGGCTGTTCGATATCACTCGTACGTTATTACAGCAGCCCGATTT
GGCCTCACTGAGCGAGGCTCTTTCGCAACTGGTAAAGCAGTCTGCGCTGGCCGACAACGCCGCCATCGTGTTGTGGCAAG
CGCAGACGCAACGTGCGTCGTACTATACGACGCATAGCAGTAGCGGTCCGATTGCTTACGAAGATGAAACCGTACTGGCT
CACGGGCCGATTAGGCGGATTTTATCGCGCCCGGATACCCTGCACTGTAACTATGCAGAATTTAGTGAAACCTGGCCGCA
ACTGGCCGCCGGTACACTTTACCCACAATTTGGACACTACTGCTTATTACCACTCGCCGCTGAAGGGCGGATTTTTGGTG
GCTGTGAATTTACCCGTGTTGATGATCGTCCGTGGAGCGAAAAAGAGTTTTCACGCCTGCAAACCTTTACCCAAATTGTC
TCAGTGGTTGCCGAGCAAATTCAAAGCCGGGTGAGTAACAATATCGACTATGACCTGCTTTGTCGTGAGCGGGATAATTT
CCGCATCCTGGTGGCGATAACCAATGCGGTGTTATCACGACTGGATATGGACGAACTGGTGAGTGAAGTTGCCAAAGAGA
TCCACCATTATTTCAATATCGATGACATCAGCATTGTTCTGCGTAGTCATCGCAAACATAAGCTCAATATCTATTCCACG
CACTATCTCGATAAACATCATCCTGCCCACGAACAGAGTGAAGTGGATGAAGCCGGAACCCTGACCGAACGGGTCTTTAA
AAGCAAAGAGATGCTGCTGATTAACCTCCACGAGCGTGATTCGCTGGCACCTTATGAACGGATGCTGTTTGAAACCTGGG
GGAATCAAATTCAAACGTTATGCCTGCTGCCATTGAAGTCGGGTAATACCATGCTCGGCGTATTGAAACTGGCACAGTGC
GAAGAGAAAGTCTTCACCACCGCTAACCTGAATCTACTGCGCCAGATTGCCGAACGTGTGGCTATCGCCGTCGATAATGC
CCTTGCCTACCAGGAAATTCATCGTTTGAAAGAGCGTCTTGTCGATGAGAACCTCGCCCTGACCGAACAACTGAATAACG
TCGAGAGTGATTTTGGTGAAATCATCGGGCGCAGCGATGCCATGTATCGGGTGATGAAACAGGTCGAGATGGTGGCGCAA
AGTGACAGTACCGTATTAATTCTTGGTGAAACTGGTACTGGTAAGGAACTGATTGCCAGAGCGATTCACAATCTGAGCGG
ACGTAATAGTCGGCGGATGGTAAAGATGAACTGCGCCGCAATGCCAGCCGGACTGCTGGAAAGCGACCTGTTTGGCCACG
AACGTGGAGCTTTTACCGGTGCCAGCACACAGCGTATTGGCCGTTTCGAACTGGCGGATAAAAGCTCGCTGTTCCTTGAT
GAAGTGGGCGATATGCCACTGGAATTGCAACCGAAGTTGCTTCGCGTGCTGCAGGAACAAGAGTTTGAACGGCTTGGCAG
TAATAAGATCATTCAGACAGATGTGCGATTGATCGCCGCAACCAACCGCGATCTACAAAAAATGGTCGCGGATCGTGAAT
TCCGTAGCGATCTTTACTACCGTCTGAACGTGTTCCCGATCCATTTACCGCCATTGCGTGAGCGACCAGAAGATATCCCG
TTGTTGGTTAAAGCGTTCACTCATAAAATTGCCCGCCGTATGGGGCGTAATATCGACAGTATTCCTGCCGAAACCCTTCG
CGCCTTGTGTGATATGGAATGGCCTGGCAATGTGCGGGAACTGGAGAACGTGATTGAACGTGCGGTGTTACTGACACGCG
GTAACGTGCTGCAACTTTCGTTACCCGATATTGCCCCAGCGACGCAAGTACAGCCTCCTGTTGAGAAAGAAGTCGCCCAG
GAAGGGGAAGACGAATATCAGCTTATTGTCCGGGTGTTGAAAGAGACAAACGGTGTGGTAGCGGGGCCGAAAGGCGCGGC
CCAGCGTCTGGGGATGAAGCGAACAACACTGCTTTCACGTATGAAACGATTAGGAATCAATAAGGATGCGCTAATCCAGG
GTATGCCATAA

Upstream 100 bases:

>100_bases
TGGGGATATTAAGCGGCATTATCATCATTCTCGATCTACTCTTTTCTTGTAGTAATTTTCTTTTAGCACCGCAGGTTGCG
GTGCATTCCTGGATAGCAAA

Downstream 100 bases:

>100_bases
TCGTTATTTACGTTAAAGGAATGGAGGCAATATGGCAGTTTCAGCACGTAATCAATTAATCGGAACAATAAGCGCACTGG
CATTGGGTGCCGTCAATGAT

Product: DNA-binding transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKQSALADNAAIVLWQAQTQRASYYTTHSSSGPIAYEDETVLA
HGPIRRILSRPDTLHCNYAEFSETWPQLAAGTLYPQFGHYCLLPLAAEGRIFGGCEFTRVDDRPWSEKEFSRLQTFTQIV
SVVAEQIQSRVSNNIDYDLLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHHYFNIDDISIVLRSHRKHKLNIYST
HYLDKHHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDSLAPYERMLFETWGNQIQTLCLLPLKSGNTMLGVLKLAQC
EEKVFTTANLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVESDFGEIIGRSDAMYRVMKQVEMVAQ
SDSTVLILGETGTGKELIARAIHNLSGRNSRRMVKMNCAAMPAGLLESDLFGHERGAFTGASTQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLQKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIP
LLVKAFTHKIARRMGRNIDSIPAETLRALCDMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIAPATQVQPPVEKEVAQ
EGEDEYQLIVRVLKETNGVVAGPKGAAQRLGMKRTTLLSRMKRLGINKDALIQGMP

Sequences:

>Translated_696_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKQSALADNAAIVLWQAQTQRASYYTTHSSSGPIAYEDETVLA
HGPIRRILSRPDTLHCNYAEFSETWPQLAAGTLYPQFGHYCLLPLAAEGRIFGGCEFTRVDDRPWSEKEFSRLQTFTQIV
SVVAEQIQSRVSNNIDYDLLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHHYFNIDDISIVLRSHRKHKLNIYST
HYLDKHHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDSLAPYERMLFETWGNQIQTLCLLPLKSGNTMLGVLKLAQC
EEKVFTTANLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVESDFGEIIGRSDAMYRVMKQVEMVAQ
SDSTVLILGETGTGKELIARAIHNLSGRNSRRMVKMNCAAMPAGLLESDLFGHERGAFTGASTQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLQKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIP
LLVKAFTHKIARRMGRNIDSIPAETLRALCDMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIAPATQVQPPVEKEVAQ
EGEDEYQLIVRVLKETNGVVAGPKGAAQRLGMKRTTLLSRMKRLGINKDALIQGMP
>Mature_695_residues
SYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKQSALADNAAIVLWQAQTQRASYYTTHSSSGPIAYEDETVLAH
GPIRRILSRPDTLHCNYAEFSETWPQLAAGTLYPQFGHYCLLPLAAEGRIFGGCEFTRVDDRPWSEKEFSRLQTFTQIVS
VVAEQIQSRVSNNIDYDLLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHHYFNIDDISIVLRSHRKHKLNIYSTH
YLDKHHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDSLAPYERMLFETWGNQIQTLCLLPLKSGNTMLGVLKLAQCE
EKVFTTANLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVESDFGEIIGRSDAMYRVMKQVEMVAQS
DSTVLILGETGTGKELIARAIHNLSGRNSRRMVKMNCAAMPAGLLESDLFGHERGAFTGASTQRIGRFELADKSSLFLDE
VGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLQKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPL
LVKAFTHKIARRMGRNIDSIPAETLRALCDMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIAPATQVQPPVEKEVAQE
GEDEYQLIVRVLKETNGVVAGPKGAAQRLGMKRTTLLSRMKRLGINKDALIQGMP

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=692, Percent_Identity=90.606936416185, Blast_Score=1293, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=567, Percent_Identity=50.6172839506173, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI1790437, Length=309, Percent_Identity=46.6019417475728, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI1788550, Length=323, Percent_Identity=47.0588235294118, Blast_Score=269, Evalue=5e-73,
Organism=Escherichia coli, GI1788905, Length=241, Percent_Identity=52.2821576763486, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1790299, Length=367, Percent_Identity=40.8719346049046, Blast_Score=242, Evalue=7e-65,
Organism=Escherichia coli, GI87082152, Length=279, Percent_Identity=45.1612903225806, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=40.6153846153846, Blast_Score=235, Evalue=8e-63,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=46.9827586206897, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1786524, Length=323, Percent_Identity=38.6996904024768, Blast_Score=201, Evalue=2e-52,
Organism=Escherichia coli, GI1787583, Length=332, Percent_Identity=35.8433734939759, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=37.007874015748, Blast_Score=142, Evalue=1e-34,
Organism=Escherichia coli, GI87081858, Length=326, Percent_Identity=31.5950920245399, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78732; Mature: 78601

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKQSALADNAAIVLWQAQTQRAS
CCCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCE
YYTTHSSSGPIAYEDETVLAHGPIRRILSRPDTLHCNYAEFSETWPQLAAGTLYPQFGHY
EEEECCCCCCEEECCCEEEEHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCE
CLLPLAAEGRIFGGCEFTRVDDRPWSEKEFSRLQTFTQIVSVVAEQIQSRVSNNIDYDLL
EEEEEECCCCEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHHYFNIDDISIVLRSHRKHKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEE
HYLDKHHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDSLAPYERMLFETWGNQIQTL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEEE
CLLPLKSGNTMLGVLKLAQCEEKVFTTANLNLLRQIAERVAIAVDNALAYQEIHRLKERL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVESDFGEIIGRSDAMYRVMKQVEMVAQSDSTVLILGETGTGKELIAR
HCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRNSRRMVKMNCAAMPAGLLESDLFGHERGAFTGASTQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCCHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLQKMVADREFRSDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEEEECCHHHHHHHHHHHHHHCCEE
RLNVFPIHLPPLRERPEDIPLLVKAFTHKIARRMGRNIDSIPAETLRALCDMEWPGNVRE
EEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LENVIERAVLLTRGNVLQLSLPDIAPATQVQPPVEKEVAQEGEDEYQLIVRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEE
AGPKGAAQRLGMKRTTLLSRMKRLGINKDALIQGMP
ECCCHHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCC
>Mature Secondary Structure 
SYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKQSALADNAAIVLWQAQTQRAS
CCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCE
YYTTHSSSGPIAYEDETVLAHGPIRRILSRPDTLHCNYAEFSETWPQLAAGTLYPQFGHY
EEEECCCCCCEEECCCEEEEHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCE
CLLPLAAEGRIFGGCEFTRVDDRPWSEKEFSRLQTFTQIVSVVAEQIQSRVSNNIDYDLL
EEEEEECCCCEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHHYFNIDDISIVLRSHRKHKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEE
HYLDKHHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDSLAPYERMLFETWGNQIQTL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEEE
CLLPLKSGNTMLGVLKLAQCEEKVFTTANLNLLRQIAERVAIAVDNALAYQEIHRLKERL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVESDFGEIIGRSDAMYRVMKQVEMVAQSDSTVLILGETGTGKELIAR
HCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRNSRRMVKMNCAAMPAGLLESDLFGHERGAFTGASTQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCCHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLQKMVADREFRSDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEEEECCHHHHHHHHHHHHHHCCEE
RLNVFPIHLPPLRERPEDIPLLVKAFTHKIARRMGRNIDSIPAETLRALCDMEWPGNVRE
EEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LENVIERAVLLTRGNVLQLSLPDIAPATQVQPPVEKEVAQEGEDEYQLIVRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEE
AGPKGAAQRLGMKRTTLLSRMKRLGINKDALIQGMP
ECCCHHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]