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The map label for this gene is pphB [H]

Identifier: 218547748

GI number: 218547748

Start: 342290

End: 342946

Strand: Reverse

Name: pphB [H]

Synonym: EFER_0333

Alternate gene names: 218547748

Gene position: 342946-342290 (Counterclockwise)

Preceding gene: 218547749

Following gene: 218547744

Centisome position: 7.47

GC content: 43.07

Gene sequence:

>657_bases
ATGCCATCTACACGCTATCAAAAAATTAATGCCCATCACTATCGCCATATATGGGCCGTTGGTGATATTCATGGTGACTA
TCAATTATTACAATCCCGCTTACACCAATTATCTTTTTGTCCCGAAACTGACTTTCTTATTTCTGTCGGCGATAACATTG
ATCGGGGGCCGGATAGCCTTAACGTGCTGCGCCTGTTAAACCAGCCCTGGTTTACCTCCGTCAAAGGCAACCACGAAGCG
ATGGCGCTTGATGCATTCGCGACTGGCGATGGTAATATGTGGTTAGCCAGCGGTGGTAACTGGTTTTTCGATTTAAATGA
TTCAGAGCAAAAAGAAGCTATAGATCTGTTGCTGAAATTTCATCACCTCCCACATATTATTGAAATCACTAACGACATCA
TAAAATATGTCATCGCACATGCGGATTATCCAGGTGATGAATATCAGTTTGGAAAAGAAATAGCAGAAAGCGAATTACTC
TGGCCTGTTGATCGTGTACTGAAATCGCTCAAAGGCGAGTTACAGGAAATAAACGGCGCCGACTGCTTTATCTTCGGTCA
TATGATGTTTGATAACATCCAGACGTTCGCCAACCAGGTATATATTGATACCGGATCGCCGGAAAGCGGGCGGCTGTCGT
TTTATAAAATCAAATAA

Upstream 100 bases:

>100_bases
ATTCCCGATAGTTTTTTGCTATCGGGAATATTAACGACAACTGACGAATCAAATAAAAATACCCTGTATAATAGGAAATC
TTATTTTACAGGGTAAAACC

Downstream 100 bases:

>100_bases
CGTGGCCGGGTAAAGCAAAGCCTCTACCCGGCCCTTTTTTATTAGCGCTTATCTGCCGCCAGCAGCGGCGGAATGCTCGG
CACCATTGGCGCGTCATCAA

Product: serine/threonine-specific protein phosphatase 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETDFLISVGDNIDRGPDSLNVLRLLNQPWFTSVKGNHEA
MALDAFATGDGNMWLASGGNWFFDLNDSEQKEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYQFGKEIAESELL
WPVDRVLKSLKGELQEINGADCFIFGHMMFDNIQTFANQVYIDTGSPESGRLSFYKIK

Sequences:

>Translated_218_residues
MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETDFLISVGDNIDRGPDSLNVLRLLNQPWFTSVKGNHEA
MALDAFATGDGNMWLASGGNWFFDLNDSEQKEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYQFGKEIAESELL
WPVDRVLKSLKGELQEINGADCFIFGHMMFDNIQTFANQVYIDTGSPESGRLSFYKIK
>Mature_217_residues
PSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETDFLISVGDNIDRGPDSLNVLRLLNQPWFTSVKGNHEAM
ALDAFATGDGNMWLASGGNWFFDLNDSEQKEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYQFGKEIAESELLW
PVDRVLKSLKGELQEINGADCFIFGHMMFDNIQTFANQVYIDTGSPESGRLSFYKIK

Specific function: Has been shown, in vitro, to act on Ser, Thr and Tyr- phosphorylated substrates [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1789090, Length=218, Percent_Identity=89.9082568807339, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI87081988, Length=217, Percent_Identity=45.6221198156682, Blast_Score=193, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 24958; Mature: 24827

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETDFLISVGDNIDRGPDSL
CCCCCCEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHH
NVLRLLNQPWFTSVKGNHEAMALDAFATGDGNMWLASGGNWFFDLNDSEQKEAIDLLLKF
HHHHHHCCCCEECCCCCCCEEEEEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHH
HHLPHIIEITNDIIKYVIAHADYPGDEYQFGKEIAESELLWPVDRVLKSLKGELQEINGA
HCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
DCFIFGHMMFDNIQTFANQVYIDTGSPESGRLSFYKIK
EEEEEHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEC
>Mature Secondary Structure 
PSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETDFLISVGDNIDRGPDSL
CCCCCEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHH
NVLRLLNQPWFTSVKGNHEAMALDAFATGDGNMWLASGGNWFFDLNDSEQKEAIDLLLKF
HHHHHHCCCCEECCCCCCCEEEEEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHH
HHLPHIIEITNDIIKYVIAHADYPGDEYQFGKEIAESELLWPVDRVLKSLKGELQEINGA
HCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
DCFIFGHMMFDNIQTFANQVYIDTGSPESGRLSFYKIK
EEEEEHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9278503 [H]