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The map label for this gene is iap [H]

Identifier: 218547730

GI number: 218547730

Start: 328260

End: 329297

Strand: Reverse

Name: iap [H]

Synonym: EFER_0313

Alternate gene names: 218547730

Gene position: 329297-328260 (Counterclockwise)

Preceding gene: 218547741

Following gene: 218547728

Centisome position: 7.18

GC content: 47.01

Gene sequence:

>1038_bases
ATGTTTTCCGCATTGCGCCACCGTACCGCTGCCCTGGCGCTCGGCGTATGCTTTATCCTCCCTGTCCAGGCTGCCACTGT
AAGACCTGGAGATATTGCCAATACTCAGACGCGACATCTCGCCACTTTCTTTCCTGGACGAATGACCGGAACACCTGCGG
AAATGTTATCTGCCGATTATATCAATCAGCAGTTTCAACAGATGGGTTATCGCAGCGATATTCGGACATTTAATAGCCGT
TATATCTTCACCGATAAAAACAAGCGTAAAAGCTGGCATAACGTGACAGGAAGTACGGTGATTGCCGCTCATGAAGGAAA
AGTGCCCCAGCAAATCATCATCATGGCGCATCTTGATACCTATGCTCCGCAAAGTGATGCAGATATTGATGCCAACCTTG
GGGGATTAACATTACAAGGTGTGGATGATAATGCCGCAGGCCTTGGTGTTATGCTTGAACTGGCAGAGCGCCTGAAAGAT
ATTCCGACTGAGTATGGGATTCGTTTTATTGCCACCAGTGGTGAAGAGGAAGGGAAATTAGGTGCTGAGAATTTACTCAA
GCGCATGAGCGATACTGAAAAGAAAAATACCCTGCTGGTGATTAATCTCGATAATTTGATCGTGGGCGATAAGCTTTATT
TTAATAGTGGCGTAAATACACCAGAAGCTGTCAGGAAATTAACCCGTGATCGAGCGCTGGCAATTGCGCGTAATCATGGG
ATTGCAGCGACAACAAATCCAGGTTTGAATAAAAACTATCCGAAAGGCACCGGGTGTTGTAACGATGCAGAAGTTTTCGA
CAAAGCCGGTATTGCGGTACTTTCGGTGGAGACGACCAACTGGAATCTTGGAAATAAGGATGGTTATCAGCAACGCTCAA
AAACACCGGCCTTCCCGATGGGAAATAGCTGGCATGATGTAAGGCTTGATAATCAACAGCATATTGATAAGGCGCTTCCT
GGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCGCTGGTGAAGGAGCTGGCAAAGGCGTCTTGA

Upstream 100 bases:

>100_bases
CCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGC
ATGGTATTTAAGGACTCACT

Downstream 100 bases:

>100_bases
TGGGGTTGAAAATGGGAGCTGGGAGTTCTACCGCAGGGGCGGGGAATTCTAAGTGATATCCATCATCGCATCCAGTGCGC
CCGGTTTATCCCCGCTGATG

Product: alkaline phosphatase isozyme conversion aminopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MFSALRHRTAALALGVCFILPVQAATVRPGDIANTQTRHLATFFPGRMTGTPAEMLSADYINQQFQQMGYRSDIRTFNSR
YIFTDKNKRKSWHNVTGSTVIAAHEGKVPQQIIIMAHLDTYAPQSDADIDANLGGLTLQGVDDNAAGLGVMLELAERLKD
IPTEYGIRFIATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGVNTPEAVRKLTRDRALAIARNHG
IAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSVETTNWNLGNKDGYQQRSKTPAFPMGNSWHDVRLDNQQHIDKALP
GRIERRCRDVMRIMLPLVKELAKAS

Sequences:

>Translated_345_residues
MFSALRHRTAALALGVCFILPVQAATVRPGDIANTQTRHLATFFPGRMTGTPAEMLSADYINQQFQQMGYRSDIRTFNSR
YIFTDKNKRKSWHNVTGSTVIAAHEGKVPQQIIIMAHLDTYAPQSDADIDANLGGLTLQGVDDNAAGLGVMLELAERLKD
IPTEYGIRFIATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGVNTPEAVRKLTRDRALAIARNHG
IAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSVETTNWNLGNKDGYQQRSKTPAFPMGNSWHDVRLDNQQHIDKALP
GRIERRCRDVMRIMLPLVKELAKAS
>Mature_345_residues
MFSALRHRTAALALGVCFILPVQAATVRPGDIANTQTRHLATFFPGRMTGTPAEMLSADYINQQFQQMGYRSDIRTFNSR
YIFTDKNKRKSWHNVTGSTVIAAHEGKVPQQIIIMAHLDTYAPQSDADIDANLGGLTLQGVDDNAAGLGVMLELAERLKD
IPTEYGIRFIATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGVNTPEAVRKLTRDRALAIARNHG
IAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSVETTNWNLGNKDGYQQRSKTPAFPMGNSWHDVRLDNQQHIDKALP
GRIERRCRDVMRIMLPLVKELAKAS

Specific function: This protein, presumably an aminopeptidase, mediates the conversion of E.coli alkaline phosphatase isozyme 1, to isozymes 2 and 3 by removing, one by one, the two amino-terminal arginine residues [H]

COG id: COG2234

COG function: function code R; Predicted aminopeptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28C subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789111, Length=345, Percent_Identity=91.8840579710145, Blast_Score=671, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007484 [H]

Pfam domain/function: PF04389 Peptidase_M28 [H]

EC number: 3.4.11.- [C]

Molecular weight: Translated: 38083; Mature: 38083

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSALRHRTAALALGVCFILPVQAATVRPGDIANTQTRHLATFFPGRMTGTPAEMLSADY
CCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHEECCCCCCCCHHHHHHHHH
INQQFQQMGYRSDIRTFNSRYIFTDKNKRKSWHNVTGSTVIAAHEGKVPQQIIIMAHLDT
HHHHHHHCCCHHHHHHCCCEEEEECCCCCCHHCCCCCCEEEEECCCCCCCEEEEEEECCC
YAPQSDADIDANLGGLTLQGVDDNAAGLGVMLELAERLKDIPTEYGIRFIATSGEEEGKL
CCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHHCEEEEEECCCCCCCC
GAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGVNTPEAVRKLTRDRALAIARNHG
CHHHHHHHHHCCCCCCEEEEEEECCEEECCEEEECCCCCCHHHHHHHHHHHHEEEEECCC
IAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSVETTNWNLGNKDGYQQRSKTPAFPM
EEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCCCCHHHHCCCCCCCC
GNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIMLPLVKELAKAS
CCCCCEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSALRHRTAALALGVCFILPVQAATVRPGDIANTQTRHLATFFPGRMTGTPAEMLSADY
CCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHEECCCCCCCCHHHHHHHHH
INQQFQQMGYRSDIRTFNSRYIFTDKNKRKSWHNVTGSTVIAAHEGKVPQQIIIMAHLDT
HHHHHHHCCCHHHHHHCCCEEEEECCCCCCHHCCCCCCEEEEECCCCCCCEEEEEEECCC
YAPQSDADIDANLGGLTLQGVDDNAAGLGVMLELAERLKDIPTEYGIRFIATSGEEEGKL
CCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHHCEEEEEECCCCCCCC
GAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGVNTPEAVRKLTRDRALAIARNHG
CHHHHHHHHHCCCCCCEEEEEEECCEEECCEEEECCCCCCHHHHHHHHHHHHEEEEECCC
IAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSVETTNWNLGNKDGYQQRSKTPAFPM
EEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCCCCHHHHCCCCCCCC
GNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIMLPLVKELAKAS
CCCCCEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Aminopeptidases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316184; 9278503; 1316900 [H]