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The map label for this gene is cysJ [H]

Identifier: 218547716

GI number: 218547716

Start: 313567

End: 315366

Strand: Direct

Name: cysJ [H]

Synonym: EFER_0298

Alternate gene names: 218547716

Gene position: 313567-315366 (Clockwise)

Preceding gene: 218547714

Following gene: 218547717

Centisome position: 6.83

GC content: 56.22

Gene sequence:

>1800_bases
ATGACGACACAGGTCCCACCTTCCGCGTTGCTTCCGTTGAACCCGGAGCAACTGGCACGCCTTCAGGCGGCCACGACCGA
TTTAACTCCCACCCAGCTTGCCTGGGTTTCTGGCTATTTCTGGGGCGTGCTCAACCAGCAGCCTGCTGCGCTTACAGCGA
CGCCAGCGCCCGCCGCAGAAATGCCGGGTATAACTATTATCTCCGCTTCGCAAACCGGCAATGCGCGCCGGGTTGCTGAA
ACATTACGCGATGATTTATTAGCGGCAAAACTGAACGTTAAGCTGGTGAACGCGGGCGACTATAAATTCAAACAAATCGC
CAGCGAAAAACTGCTCATCGTGGTGACGTCAACGCAAGGTGAAGGGGAACCGCCGGAAGAGGCCGTCGCGCTGCATAAGT
TCCTGTTCTCCAAAAAAGCGCCGAAGCTGGAAAACACCGCGTTTGCCGTGTTTAGTCTCGGCGATAGCTCTTATGAATTT
TTCTGCCAGTCCGGGAAAGATTTCGATAGCAAGCTGGCGGAACTGGGCGGTGAACGCCTGCTCGACCGTGTCGATGCCGA
CGTTGAATACCAGGCTGCTGCCAGCGAGTGGCGCGCCCGCGTGGTCGATGTTCTTAAATCGCGCGCGCCTGTCGCAGCAC
CTTCACAATCCGTCGCTACTGGCGCGGTAAATGAAATCCACACCAGCCCGTACAGCAAAGACGCGCCGCTGGTGGCGAGC
CTTTCGGTTAACCAGAAAATTACCGGGCGTAACTCTGAAAAAGACGTTCGCCATATCGAAATTGACTTAGGTGACTCTGG
CCTGCGTTATCAGCCGGGTGACGCGCTGGGCGTCTGGTATCAGAACGATCCGGCACTGGTGAAAGAACTTGTCGAACTGC
TGTGGCTGAAAGGTGATGAACCTGTCACCGTTGAGGGCAAAACCCTGCCGCTGAATGAGGCGCTACAGTGGCATTTTGAA
CTCACCGTCAACACCGCCAACATCGTTGAGAACTACGCCACGCTAACCCGCAGTGAAACGCTGCTGCCGCTGGTGGGCGA
TAAATCGAAGTTACAGCATTACGCCGCGACGACGCCGATTGTCGACATGGTGCGTTTTTCTCCGGCACAGCTTGACGCTG
AAGCCCTGATTAACTTGCTGCGCCCGCTGACGCCGCGCCTGTACTCCATCGCCTCCTCGCAGGCGGAAGTCGAGAACGAA
GTACACGTCACCGTTGGTGTGGTGCGTTATGACATTGAAGGCCGCGCCCGTGCTGGCGGTGCCTCCAGCTTCCTCGCGGA
CCGTGTGGAAGAAGAGGGCGAAGTCCGCGTCTTTATCGAACATAACGATAACTTCCGCCTGCCCGCTAACCCGGAAACCC
CGGTGATTATGATTGGTCCGGGCACCGGTATTGCGCCGTTCCGCGCCTTTATGCAGCAACGTGCTGCTGACGAAGCGCCG
GGCAAAAACTGGCTGTTCTTTGGCAATCCGCACTTTACGGAAGATTTCCTCTACCAGGTGGAGTGGCAGCGCTACGTCAA
AGAGGGCGTGCTGACACGTATCGATCTTGCCTGGTCGCGCGATCAAAAAGAAAAAGTTTACGTACAAGACAAACTGCGCG
AACAGGGCGCAGAGCTGTGGCGCTGGATCAATGACGGTGCCCACATTTATGTCTGCGGCGACGCTAATCGCATGGCGAAA
GACGTTGAACAGGCACTTCTGGAAGTGATTGCCGAATTTGGTGGCATGGACACCGAAGCGGCGGATGAATTTTTAAGTGA
GCTGCGCGTAGAGCGCCGTTATCAGCGAGATGTCTACTAA

Upstream 100 bases:

>100_bases
TTAATCCACACCGTTCATCCCGTTAACCTTACCTTCTCTTCTGTTTTATGGGCGCTGACAGGGCGCAGAAACAGCTTTGC
TTACTGGAACATAACGACGC

Downstream 100 bases:

>100_bases
TGAACGAAAAACATCCAGGGCCTTTAGTGGTCGAAGGAAAACTGACAGACGCCGAGCGCATGAAGCTTGAGAGCAACTAC
CTGCGCGGGACCATTGCGGA

Product: sulfite reductase subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALTATPAPAAEMPGITIISASQTGNARRVAE
TLRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDVLKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFE
LTVNTANIVENYATLTRSETLLPLVGDKSKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENE
VHVTVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAK
DVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY

Sequences:

>Translated_599_residues
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALTATPAPAAEMPGITIISASQTGNARRVAE
TLRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDVLKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFE
LTVNTANIVENYATLTRSETLLPLVGDKSKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENE
VHVTVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAK
DVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
>Mature_598_residues
TTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALTATPAPAAEMPGITIISASQTGNARRVAET
LRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFF
CQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDVLKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVASL
SVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFEL
TVNTANIVENYATLTRSETLLPLVGDKSKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEV
HVTVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAPG
KNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKD
VEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=601, Percent_Identity=30.4492512479201, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI7657393, Length=601, Percent_Identity=29.783693843594, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI221316709, Length=601, Percent_Identity=29.783693843594, Blast_Score=225, Evalue=9e-59,
Organism=Homo sapiens, GI24041029, Length=597, Percent_Identity=30.9882747068677, Blast_Score=225, Evalue=1e-58,
Organism=Homo sapiens, GI221316705, Length=610, Percent_Identity=29.344262295082, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI10835173, Length=646, Percent_Identity=29.8761609907121, Blast_Score=206, Evalue=6e-53,
Organism=Homo sapiens, GI221316707, Length=531, Percent_Identity=29.9435028248588, Blast_Score=202, Evalue=7e-52,
Organism=Homo sapiens, GI40254422, Length=649, Percent_Identity=27.4268104776579, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI169790956, Length=437, Percent_Identity=28.604118993135, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI169790958, Length=437, Percent_Identity=28.604118993135, Blast_Score=161, Evalue=2e-39,
Organism=Escherichia coli, GI1789123, Length=599, Percent_Identity=98.8313856427379, Blast_Score=1218, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=636, Percent_Identity=29.2452830188679, Blast_Score=233, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17566446, Length=583, Percent_Identity=26.5866209262436, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531441, Length=394, Percent_Identity=25.3807106598985, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321832, Length=640, Percent_Identity=28.90625, Blast_Score=209, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6321143, Length=397, Percent_Identity=31.4861460957179, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6325305, Length=626, Percent_Identity=23.482428115016, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17137192, Length=600, Percent_Identity=28.8333333333333, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24582192, Length=511, Percent_Identity=30.5283757338552, Blast_Score=199, Evalue=6e-51,
Organism=Drosophila melanogaster, GI24583543, Length=524, Percent_Identity=32.2519083969466, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI78706876, Length=524, Percent_Identity=32.2519083969466, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI78706872, Length=524, Percent_Identity=32.2519083969466, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24660907, Length=595, Percent_Identity=26.0504201680672, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24660903, Length=595, Percent_Identity=26.0504201680672, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 66385; Mature: 66253

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALTATPAPAAE
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHH
MPGITIISASQTGNARRVAETLRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQG
CCCEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHCCCCEEEEEECCCC
EGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFFCQSGKDFDSKLAELGGERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCHHHHHHHHCHHHH
LDRVDADVEYQAAASEWRARVVDVLKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDE
EECCCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVTVEGKTLPLNEALQWHFELTVNTANIVENYATLTRSETLLPLVGDKSKLQHYAATTPI
CEEECCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCH
VDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEVHVTVGVVRYDIEGRARAGG
HHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHCCHHHCCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
HHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RWINDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPAALTATPAPAAE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHH
MPGITIISASQTGNARRVAETLRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQG
CCCEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHCCCCEEEEEECCCC
EGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFFCQSGKDFDSKLAELGGERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCHHHHHHHHCHHHH
LDRVDADVEYQAAASEWRARVVDVLKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLKGDE
EECCCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVTVEGKTLPLNEALQWHFELTVNTANIVENYATLTRSETLLPLVGDKSKLQHYAATTPI
CEEECCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCH
VDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEVHVTVGVVRYDIEGRARAGG
HHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHCCHHHCCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADEAP
HHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RWINDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA