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The map label for this gene is gudX [H]

Identifier: 218547695

GI number: 218547695

Start: 289716

End: 291056

Strand: Direct

Name: gudX [H]

Synonym: EFER_0275

Alternate gene names: 218547695

Gene position: 289716-291056 (Clockwise)

Preceding gene: 218547691

Following gene: 218547696

Centisome position: 6.31

GC content: 53.47

Gene sequence:

>1341_bases
ATGACAACACAATCCAGTCCCATTATTACCGATATGAAAGTCATTCCGGTCGCCGGACATGACAGCATGTTGCTTAATAT
TGGTGGTGCACATAACGCATATTTCACCCGCAATATTGTGGTACTCACCGATAACGCCGGGAATACCGGCATTGGTGAAG
CACCGGGCGGAGAGGTGATTTATCAGACACTGGTCGATGCTATTCCGATGGTGCTGGGCCAGGAGATCGCGCGACTGAAT
AAATTGGTCCAGCAGGTGCATAAAGGTAATCAGGCTGCCGATTTTGATACCTTCGGCAAAGGCGTCTGGACTTTTGAATT
GCGCGTTAATGCCGTGGCGGCGCTGGAAGCTGCCTTGCTTGACCTGTTAGGTAAGGCGCTGAATGTTCCAGTCTGCGAAC
TGTTAGGGCCAGGCAAACAACGCGATGCTATTATCGTCCTCGGTTATCTGTTTTATATCGGTGATCGGACTAAAACCGAT
CTTCCTTATCTGGAAAATACGCCGGGCAATCATGAGTGGTATCAGTTGCGGCATCAGAAAGCGATGAACAGCGAAGCCGT
TGTGCGTCTGGCAGAAGCTTCACAGGATCGCTATGGTTTTAAAGATTTCAAACTTAAGGGCGGCGTGTTACCTGGCGAGC
AAGAAATCGACACTGTTCGTGCATTGAAGAAACGCTTCCCGGATGCACGAATTACCGTTGATCCCAACGGTGCATGGCTG
CTGGATGAAGCCATTTCTCTGTGCAAAGGGCTGAACGATGTGCTGACCTATGCGGAAGATCCATGCGGCGCAGAACAGGG
TTTCTCCGGACGTGAAGTGATGGCGGAGTTTCGGCGGGCGACCGGCTTGCCCGTCGCGACCAACATGATTGCCACCAACT
GGCGCGAAATGGGCCATGCGGTAATGCTCAATGCAGTAGATATTCCACTTGCCGATCCGCATTTCTGGACGCTTTCCGGT
GCAGTTCGCGTGGCGCAGCTTTGCGACGACTGGGGGCTGACCTGGGGCTGCCATTCCAATAATCACTTCGATATCTCTCT
GGCGATGTTTACCCATGTGGGCGCGGCGGCACCGGGTAATCCTACCGCTATAGATACCCACTGGATTTGGCAGGAGGGGG
GTTGTCGCCTGACCAAAAATCCGCTGGAGATTAAAAACGGAAAAATTGCCGTTCCTGATGCGCCCGGCCTGGGCGTGGAA
CTGGACTGGGAACAGGTACAGAAGGCGCATGAAGCCTATAAACGTCTGCCTGGCGGTGCGCGTAACGACGCAGGCCCGAT
GCAGTATCTGATCCCCGGCTGGACCTTTGACCGTAAACGCCCCGTTTTCGGCCGTCACTGA

Upstream 100 bases:

>100_bases
TAATATTTCATTTTGTAGTGTGCTGTTGGTTATATCGAATTATCAGCCACCGGAGAATGATATTAAGCATACCGAATCCA
AACCTGTCACAGGGCAAAAA

Downstream 100 bases:

>100_bases
TTTTGAATAAGGACATAGATATGAGTTCTCAATTTACGACGCCTGTTGTTACTGAAATGCAGGTCATCCCGGTGGCGGGT
CATGACAGTATGCTGATGAA

Product: glucarate dehydratase

Products: 5-dehydro-4-deoxy-D-glucarate; H2O

Alternate protein names: GDH-RP; GlucDRP [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTTQSSPIITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTDNAGNTGIGEAPGGEVIYQTLVDAIPMVLGQEIARLN
KLVQQVHKGNQAADFDTFGKGVWTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAIIVLGYLFYIGDRTKTD
LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKRFPDARITVDPNGAWL
LDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSG
AVRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGGCRLTKNPLEIKNGKIAVPDAPGLGVE
LDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH

Sequences:

>Translated_446_residues
MTTQSSPIITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTDNAGNTGIGEAPGGEVIYQTLVDAIPMVLGQEIARLN
KLVQQVHKGNQAADFDTFGKGVWTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAIIVLGYLFYIGDRTKTD
LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKRFPDARITVDPNGAWL
LDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSG
AVRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGGCRLTKNPLEIKNGKIAVPDAPGLGVE
LDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH
>Mature_445_residues
TTQSSPIITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTDNAGNTGIGEAPGGEVIYQTLVDAIPMVLGQEIARLNK
LVQQVHKGNQAADFDTFGKGVWTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAIIVLGYLFYIGDRTKTDL
PYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKRFPDARITVDPNGAWLL
DEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGA
VRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGGCRLTKNPLEIKNGKIAVPDAPGLGVEL
DWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH

Specific function: Does not seem to have an in-vivo activity on glucarate or idarate. Its real substrate is unknown [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789151, Length=446, Percent_Identity=97.3094170403588, Blast_Score=878, Evalue=0.0,
Organism=Escherichia coli, GI1789150, Length=448, Percent_Identity=62.7232142857143, Blast_Score=588, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.2.1.40

Molecular weight: Translated: 48895; Mature: 48764

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQSSPIITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTDNAGNTGIGEAPGGEVI
CCCCCCCCEECEEEEEECCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHH
YQTLVDAIPMVLGQEIARLNKLVQQVHKGNQAADFDTFGKGVWTFELRVNAVAALEAALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCEEEEEEEEHHHHHHHHHHH
DLLGKALNVPVCELLGPGKQRDAIIVLGYLFYIGDRTKTDLPYLENTPGNHEWYQLRHQK
HHHHHHHCCCHHHHCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKRFPDARITVDPNGAWL
HCCHHHHEEEHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCH
LDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHA
HHHHHHHHHCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCE
VMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGN
EEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEHHHHCCCCCCCC
PTAIDTHWIWQEGGCRLTKNPLEIKNGKIAVPDAPGLGVELDWEQVQKAHEAYKRLPGGA
CCEEEEEEEEECCCCEEECCCCEECCCEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCC
RNDAGPMQYLIPGWTFDRKRPVFGRH
CCCCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTQSSPIITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTDNAGNTGIGEAPGGEVI
CCCCCCCEECEEEEEECCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHH
YQTLVDAIPMVLGQEIARLNKLVQQVHKGNQAADFDTFGKGVWTFELRVNAVAALEAALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCEEEEEEEEHHHHHHHHHHH
DLLGKALNVPVCELLGPGKQRDAIIVLGYLFYIGDRTKTDLPYLENTPGNHEWYQLRHQK
HHHHHHHCCCHHHHCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKRFPDARITVDPNGAWL
HCCHHHHEEEHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCH
LDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHA
HHHHHHHHHCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCE
VMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGN
EEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEHHHHCCCCCCCC
PTAIDTHWIWQEGGCRLTKNPLEIKNGKIAVPDAPGLGVELDWEQVQKAHEAYKRLPGGA
CCEEEEEEEEECCCCEEECCCCEECCCEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCC
RNDAGPMQYLIPGWTFDRKRPVFGRH
CCCCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glucarate

Specific reaction: D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]