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The map label for this gene is ygdE [H]

Identifier: 218547682

GI number: 218547682

Start: 275415

End: 276515

Strand: Direct

Name: ygdE [H]

Synonym: EFER_0261

Alternate gene names: 218547682

Gene position: 275415-276515 (Clockwise)

Preceding gene: 218547681

Following gene: 218547688

Centisome position: 6.0

GC content: 48.86

Gene sequence:

>1101_bases
ATGAATAAGGTTGTATTGTTGTGTCGCCCAGGCTTCGAAAAAGAGTGTGCCGCAGAAATCACGGACAAAGCAGGGCGACA
AGAGATTTTCGGTTTTGCTCGTGTGAAAGAGAATGCGGGTTATGTCATTTATGAATGTTATCAAGCTGAAGATGGCGATA
AATTAATTCGTGAATTGCCATTCAGCTCGTTGATCTTTGCCCGTCAGTGGTTTGTGGTGGGCGAGTTGCTTCAGCATTTA
CCACCAGAAGATCGCATTACCCCTATAGTCGGCATGTTGCAGGGTGTGGTGGAGAAAGGCGGTGAGTTGCGTGTTGAAGT
TGCCGATACCAACGAAAGCAAAGAACTGCTCAAATTCTGTCGTAAATTCACGGTGCCATTACGTGCAGCATTGCGTGATG
CAGGTGTCCTTGCGAACTATGAAACGCCGAAGCGTCCGGTGGTGCATGTGTTCTTTATTGCACCAGGTTGCTGCTATACC
GGTTATTCTTACAGCAATAATAATTCACCGTTCTATATGGGCATTCCGCGTCTGAAGTTCCCGGCAGATGCACCAAGCCG
TTCCACGCTCAAACTGGAAGAGGCATTTCATGTATTTATTCCGGCAGATGAGTGGGATGAACGTCTGGCAAACGGAATGT
GGGCAGTGGATCTGGGGGCTTGCCCTGGCGGTTGGACTTACCAGTTAGTGAAGCGCAACATGTGGGTATATTCCGTTGAT
AACGGCCCGATGGCGCAAAGTTTGATGGATACCGGCCAGGTCACATGGCTACGGGAAGATGGTTTTAAATTCCGTCCGAC
GCGTAACAACATTTCCTGGATGGTGTGCGATATGGTTGAGAAACCTGCAAAAGTAGCTGCACTGATGGCGCAATGGCTGG
TTAATGGCTGGTGCCGTGAAACCATCTTCAACCTTAAACTGCCAATGAAAAAACGCTACGAAGAAGTGTCACATAATCTG
GCGTACATTCAGGCTCAGCTTGATGAACATGGCATTAATGCCCAGATTCAGGCGCGACAGTTGTATCACGATCGCGAAGA
AGTGACGGTGCATGTCCGTCGTATATGGGCTGCTGTTGGTGGTCGTCGCGACGAACGCTAA

Upstream 100 bases:

>100_bases
CTGTGGGCGTATGTTACTCCTGTCGGTGGCGTTAGCTTTCTGGCAGGTTGGGTTCTCATGTTAATTGGCGCTATCCGTTT
GAAGCGTAAGGGCGTAAGTC

Downstream 100 bases:

>100_bases
TCTCTGACTACCTGTGCCGGAAGCTGTTACTGTCTGCTTCCGGCATTGGTTTATCAGCGTTTAAGTCTTAGCTGTTGTAA
ATTGCCATCAATATGCACAT

Product: RNA 2'-O-ribose methyltransferase

Products: NA

Alternate protein names: 23S rRNA 2'-O-ribose methyltransferase rlmM [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MNKVVLLCRPGFEKECAAEITDKAGRQEIFGFARVKENAGYVIYECYQAEDGDKLIRELPFSSLIFARQWFVVGELLQHL
PPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
NGPMAQSLMDTGQVTWLREDGFKFRPTRNNISWMVCDMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNL
AYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVGGRRDER

Sequences:

>Translated_366_residues
MNKVVLLCRPGFEKECAAEITDKAGRQEIFGFARVKENAGYVIYECYQAEDGDKLIRELPFSSLIFARQWFVVGELLQHL
PPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
NGPMAQSLMDTGQVTWLREDGFKFRPTRNNISWMVCDMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNL
AYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVGGRRDER
>Mature_366_residues
MNKVVLLCRPGFEKECAAEITDKAGRQEIFGFARVKENAGYVIYECYQAEDGDKLIRELPFSSLIFARQWFVVGELLQHL
PPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
NGPMAQSLMDTGQVTWLREDGFKFRPTRNNISWMVCDMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNL
AYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVGGRRDER

Specific function: Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA [H]

COG id: COG2933

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family. RlmM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789171, Length=366, Percent_Identity=98.6338797814208, Blast_Score=756, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011224
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: NA

Molecular weight: Translated: 41921; Mature: 41921

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVVLLCRPGFEKECAAEITDKAGRQEIFGFARVKENAGYVIYECYQAEDGDKLIRELP
CCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHEEECCCCEEEEEEEECCCHHHHHHHCC
FSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFC
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
RKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYTGYSYSNNNSPFYMGIPRLKF
HHHCHHHHHHHHHCCEEECCCCCCCCEEEEEEECCCCEECCCCCCCCCCCEEEECCCEEC
PADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
CCCCCCCCEEEEECEEEEEECCCHHHHHHHCCEEEEEECCCCCCCCHHEECCCEEEEEEC
NGPMAQSLMDTGQVTWLREDGFKFRPTRNNISWMVCDMVEKPAKVAALMAQWLVNGWCRE
CCHHHHHHHCCCCEEEEECCCCEECCCCCCCCEEEEHHHHCHHHHHHHHHHHHHHHHHHH
TIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVG
HHHHCCCCHHHHHHHHHCCCEEEEEEHHCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHC
GRRDER
CCCCCC
>Mature Secondary Structure
MNKVVLLCRPGFEKECAAEITDKAGRQEIFGFARVKENAGYVIYECYQAEDGDKLIRELP
CCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHEEECCCCEEEEEEEECCCHHHHHHHCC
FSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFC
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
RKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYTGYSYSNNNSPFYMGIPRLKF
HHHCHHHHHHHHHCCEEECCCCCCCCEEEEEEECCCCEECCCCCCCCCCCEEEECCCEEC
PADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
CCCCCCCCEEEEECEEEEEECCCHHHHHHHCCEEEEEECCCCCCCCHHEECCCEEEEEEC
NGPMAQSLMDTGQVTWLREDGFKFRPTRNNISWMVCDMVEKPAKVAALMAQWLVNGWCRE
CCHHHHHHHCCCCEEEEECCCCEECCCCCCCCEEEEHHHHCHHHHHHHHHHHHHHHHHHH
TIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVG
HHHHCCCCHHHHHHHHHCCCEEEEEEHHCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHC
GRRDER
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA