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The map label for this gene is argA [H]

Identifier: 218547673

GI number: 218547673

Start: 266249

End: 267580

Strand: Reverse

Name: argA [H]

Synonym: EFER_0252

Alternate gene names: 218547673

Gene position: 267580-266249 (Counterclockwise)

Preceding gene: 218547677

Following gene: 218547671

Centisome position: 5.83

GC content: 48.42

Gene sequence:

>1332_bases
GTGGTAAAGGAGCGTAGTACCGAACTTGTTCAGGGGTTCCGTCATTCTGTTCCCTATATCAACACCCATCGGGGAAAGAC
GTTTGTCATTATGCTCGGCGGCGAAGCCATTGAGCATGAGAACTTCTCCAGTATTGTCAATGATATTGGCTTATTACATA
GCCTCGGAATTCGTCTGGTTGTAGTTTATGGAGCCCGACCACAAATCGATGCCAACCTTGCAGCGCATCAACATGAGCCG
CTTTATCACAAGAATATCCGCGTAACAGATGCCAAAACGCTGGAACTGGTTAAACAGGCTGCTGGCACATTACAATTGGA
TATCACCGCTCGCCTGTCGATGAGTCTAAATAACACGCCGTTACAGGGCGCACATATTAATGTGGTCAGCGGTAATTTCA
TCATTGCGCAGCCACTGGGCGTTGATGATGGTGTGGATTATTGTCATAGCGGACGTATTCGCCGTATTGATGAAGAAGCG
ATCCACCGCCAACTGGACAGTGGTGCAATCGTGTTGCTCGGACCTGTGGCCGTTTCTGTCACTGGCGAAAGTTTTAACCT
GACCTCGGAAGAAATTGCGACCCAACTCGCCATTAAACTGAAAGCAGAGAAAATGATCGGTTTCTGCTCATCTCAGGGCG
TGACTAATGACGATGGCGATATTGTATCTGAACTCTTTCCTAACGAAGCTCAGGCGCGTGTAGAAGCACAGGAAACGGCT
GGCGATTATAACTCAGGCACCGTACGCTTTTTACGCGGTGCGGTGAAGGCCTGCCGCAGTGGCGTGCGGCGTTGCCATTT
AATTAGCTATCAAGAAGATGGCGCACTACTACAAGAACTGTTCTCCCGTGACGGTATTGGTACGCAGATTGTTATGGAAA
GCGCAGAACAAATCCGCCGCGCCACTATTAATGATATTGGCGGTATTTTGGAGTTGATTCGCCCGCTGGAACAACAGGGA
ATTCTGGTACGTCGCTCGCGCGAACAACTGGAAATGGAGATCGATAAATTTACTATTATTCAGCGTGATAACACGACTAT
TGCCTGTGCCGCGCTCTATCCATTCCCGGAAGAGAAGATAGGCGAAATGGCATGTGTGGCGGTACACCCGGATTACCGCA
GTTCAGCTCGCGGTGAAGTCTTACTTGAACGAATTGCTGCACAGGCCAAACAGATAGGATTAAGCAAACTATTTGTCCTT
ACTACCCGTAGTATTCACTGGTTTCAGGAACGCGGGTTTACACCTGTGGATATTGATTTGCTGCCAGAAAGCAAAAAGTT
GATGTACAACTATCAGCGTCGTTCCAAAGTGTTAATGGCGGATTTGGGCTAG

Upstream 100 bases:

>100_bases
GATAAAAAACTGCAGACCGGGCAAAATTTGCGCTTGCATGGTGGAGAAAAACAGAATAAAAATACAATAATTTCGAATAT
TCATGCAAAGAGGGCGAGCC

Downstream 100 bases:

>100_bases
TCCGGTTTGGTGCCGGATATGAATGATATCCGGCGCTTCGAGTGTCACACACGCTCTGCTGGCTACAGGATCGTTTCTTC
AGTCAAAATCCGTCTAGCCC

Product: N-acetylglutamate synthase

Products: NA

Alternate protein names: N-acetylglutamate synthase; AGS; NAGS [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MVKERSTELVQGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLVVVYGARPQIDANLAAHQHEP
LYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEEA
IHRQLDSGAIVLLGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQETA
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQG
ILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSARGEVLLERIAAQAKQIGLSKLFVL
TTRSIHWFQERGFTPVDIDLLPESKKLMYNYQRRSKVLMADLG

Sequences:

>Translated_443_residues
MVKERSTELVQGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLVVVYGARPQIDANLAAHQHEP
LYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEEA
IHRQLDSGAIVLLGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQETA
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQG
ILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSARGEVLLERIAAQAKQIGLSKLFVL
TTRSIHWFQERGFTPVDIDLLPESKKLMYNYQRRSKVLMADLG
>Mature_443_residues
MVKERSTELVQGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLVVVYGARPQIDANLAAHQHEP
LYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEEA
IHRQLDSGAIVLLGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQETA
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQG
ILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSARGEVLLERIAAQAKQIGLSKLFVL
TTRSIHWFQERGFTPVDIDLLPESKKLMYNYQRRSKVLMADLG

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1789181, Length=443, Percent_Identity=97.2911963882619, Blast_Score=889, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320913, Length=290, Percent_Identity=22.0689655172414, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR001048
- InterPro:   IPR010167 [H]

Pfam domain/function: PF00696 AA_kinase; PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.1 [H]

Molecular weight: Translated: 49097; Mature: 49097

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKERSTELVQGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLV
CCCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEE
VVYGARPQIDANLAAHQHEPLYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTP
EEECCCCCCCCCCCCCCCCCCEECCEEEECHHHHHHHHHHCCEEEEEEEEEEEEEECCCC
LQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEEAIHRQLDSGAIVLLGPVAVSV
CCCCEEEEEECCEEEEECCCCCCCCHHHCCCCCEECCHHHHHHHCCCCCEEEEEEEEEEE
TGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQETA
ECCCCCCCHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHEEHHHHC
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRR
CCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
ATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKI
HHHHHHHHHHHHHHHHHHCCEEEECCHHHHHEEECEEEEEEECCCEEEEEEECCCCHHHH
GEMACVAVHPDYRSSARGEVLLERIAAQAKQIGLSKLFVLTTRSIHWFQERGFTPVDIDL
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEEECCHHHHHHCCCCEEEEEE
LPESKKLMYNYQRRSKVLMADLG
CCCCHHHHHHHHHHCEEEEECCC
>Mature Secondary Structure
MVKERSTELVQGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLV
CCCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEE
VVYGARPQIDANLAAHQHEPLYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTP
EEECCCCCCCCCCCCCCCCCCEECCEEEECHHHHHHHHHHCCEEEEEEEEEEEEEECCCC
LQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEEAIHRQLDSGAIVLLGPVAVSV
CCCCEEEEEECCEEEEECCCCCCCCHHHCCCCCEECCHHHHHHHCCCCCEEEEEEEEEEE
TGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQETA
ECCCCCCCHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHEEHHHHC
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRR
CCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
ATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKI
HHHHHHHHHHHHHHHHHHCCEEEECCHHHHHEEECEEEEEEECCCEEEEEEECCCCHHHH
GEMACVAVHPDYRSSARGEVLLERIAAQAKQIGLSKLFVLTTRSIHWFQERGFTPVDIDL
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEEECCHHHHHHCCCCEEEEEE
LPESKKLMYNYQRRSKVLMADLG
CCCCHHHHHHHHHHCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]