BacMap

Definition	Escherichia fergusonii ATCC 35469 chromosome, complete genome.
Accession	NC_011740
Length	4,588,711

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The map label for this gene is dkgB [H]

Identifier: 218547657

GI number: 218547657

Start: 250591

End: 251394

Strand: Direct

Name: dkgB [H]

Synonym: EFER_0236

Alternate gene names: 218547657

Gene position: 250591-251394 (Clockwise)

Preceding gene: 218547656

Following gene: 218547659

Centisome position: 5.46

GC content: 45.4

Gene sequence:

>804_bases
ATGGCTATCCCTGCATTTGGTTTAGGTACTTTCCGTCTTAAAGACGACGTTGTTATTGCATCAGTGAAAACGGCACTTGA
ACTTGGTTATCGTGCAATTGATACCGCACAAATCTATGATAACGAAGCTGCAGTAGGTCAGGCGATTGCAGAAAGTGGCG
TGCCTCGTAATGAACTCTATATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAACTGATCCCGAGTCTGAAA
GAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGGCGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGT
TGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGTCTGACGTGTGAGATCGGTATTTCCAACTTCACGATCC
CATTGATGGAAAAAGCGATTGCTGCTGTTGGCGCTGAAAATATCGCTACCAACCAGATTGAACTCTCTCCTTATCTGCAA
AACCGTAAAGTAGTCGATTGGGCTAAACAGCACGGTATCCATATTACTTCCTATATGACGCTGGCATATGGGAAGGCCCT
GAAAGATGAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGG
AAGGTTACTCAGTAATTCCTTCTTCTACTAAACGTGAAAACCTGGAGAGTAATCTTAAGGCACAAAATTTACAGTTTGAT
GCCGAAGATAAAAAAGCGATCGCCGCACTGGATTGCAACGACCGCCTGGTAAGCCCGGAAGGTCTGGCTCCTGAATGGGA
TTAA

Upstream 100 bases:

>100_bases
ATAATCGCAGAAATCCTCTGCGTTTTACGCTCTTTTTCCGCAACAGTCTGAAGTCCATAATCATCTCAGTAAACGAAAAT
AGCATTAAAAGAGACATATT

Downstream 100 bases:

>100_bases
GCCTCTCTAACAGCTCCTTTGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTAC
CGCACGGTCGCTGTAATAGA

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MAIPAFGLGTFRLKDDVVIASVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRNELYITTKIWIENLSKDKLIPSLK
ESLQKLRTDYVDLALIHWPSPNDEVSVEEFMQALLEAKKQGLTCEIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
NRKVVDWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQFD
AEDKKAIAALDCNDRLVSPEGLAPEWD

Sequences:

>Translated_267_residues
MAIPAFGLGTFRLKDDVVIASVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRNELYITTKIWIENLSKDKLIPSLK
ESLQKLRTDYVDLALIHWPSPNDEVSVEEFMQALLEAKKQGLTCEIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
NRKVVDWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQFD
AEDKKAIAALDCNDRLVSPEGLAPEWD
>Mature_266_residues
AIPAFGLGTFRLKDDVVIASVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRNELYITTKIWIENLSKDKLIPSLKE
SLQKLRTDYVDLALIHWPSPNDEVSVEEFMQALLEAKKQGLTCEIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQN
RKVVDWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQFDA
EDKKAIAALDCNDRLVSPEGLAPEWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI310109922, Length=275, Percent_Identity=32, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI310109920, Length=292, Percent_Identity=30.4794520547945, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI45446745, Length=292, Percent_Identity=30.4794520547945, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4503285, Length=292, Percent_Identity=30.4794520547945, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=30.4498269896194, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=30.4498269896194, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=30.1369863013699, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=29.553264604811, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=30.6397306397306, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=31.0580204778157, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=30.1754385964912, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=29.7658862876254, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI300116273, Length=269, Percent_Identity=30.8550185873606, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=30.5755395683453, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI291291012, Length=270, Percent_Identity=28.8888888888889, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=37.719298245614, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI207028673, Length=114, Percent_Identity=37.719298245614, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=97.0037453183521, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI87082198, Length=256, Percent_Identity=37.890625, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1788081, Length=249, Percent_Identity=29.718875502008, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI48994888, Length=267, Percent_Identity=27.7153558052434, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787674, Length=263, Percent_Identity=28.1368821292776, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17550248, Length=271, Percent_Identity=35.7933579335793, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=35.5172413793103, Blast_Score=140, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=35.4166666666667, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71998625, Length=264, Percent_Identity=30.3030303030303, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17552492, Length=267, Percent_Identity=31.0861423220974, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17561298, Length=276, Percent_Identity=30.4347826086957, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17561300, Length=275, Percent_Identity=30.1818181818182, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17566692, Length=262, Percent_Identity=32.0610687022901, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17538386, Length=281, Percent_Identity=29.5373665480427, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=26.1904761904762, Blast_Score=113, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17564128, Length=291, Percent_Identity=26.4604810996564, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17562292, Length=268, Percent_Identity=28.3582089552239, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324694, Length=289, Percent_Identity=35.2941176470588, Blast_Score=159, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6320576, Length=282, Percent_Identity=36.5248226950355, Blast_Score=152, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=29.4117647058824, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322556, Length=259, Percent_Identity=26.6409266409266, Blast_Score=116, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319625, Length=275, Percent_Identity=30.5454545454545, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320079, Length=274, Percent_Identity=31.021897810219, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24644950, Length=293, Percent_Identity=32.7645051194539, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24662789, Length=290, Percent_Identity=30.6896551724138, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI221378297, Length=313, Percent_Identity=30.9904153354633, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=30.6620209059233, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=30.7692307692308, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=34.2560553633218, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21356425, Length=289, Percent_Identity=30.7958477508651, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=29.1228070175439, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=33.5640138408304, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45553081, Length=284, Percent_Identity=28.169014084507, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281366140, Length=259, Percent_Identity=26.2548262548263, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 29409; Mature: 29278

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPAFGLGTFRLKDDVVIASVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRNELY
CCCCCCCCEEEEEECCEEEHHHHHHHHHCHHHCCHHHHCCCHHHHHHHHHHCCCCCCCEE
ITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLALIHWPSPNDEVSVEEFMQALLEAKKQ
EEEEHEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC
GLTCEIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVDWAKQHGIHITSYMT
CCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCHHHHHHHHCCEEEEHHHH
LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQFD
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCCHHHHHHHCCCCCCCEEC
AEDKKAIAALDCNDRLVSPEGLAPEWD
CCCCCEEEEECCCCCEECCCCCCCCCC
>Mature Secondary Structure 
AIPAFGLGTFRLKDDVVIASVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRNELY
CCCCCCCEEEEEECCEEEHHHHHHHHHCHHHCCHHHHCCCHHHHHHHHHHCCCCCCCEE
ITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLALIHWPSPNDEVSVEEFMQALLEAKKQ
EEEEHEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC
GLTCEIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVDWAKQHGIHITSYMT
CCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCHHHHHHHHCCEEEEHHHH
LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQFD
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCCHHHHHHHCCCCCCCEEC
AEDKKAIAALDCNDRLVSPEGLAPEWD
CCCCCEEEEECCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008 [H]