Click here to switch to the map view.

The map label for this gene is ligT [H]

Identifier: 218547603

GI number: 218547603

Start: 183487

End: 184242

Strand: Reverse

Name: ligT [H]

Synonym: EFER_0170

Alternate gene names: 218547603

Gene position: 184242-183487 (Counterclockwise)

Preceding gene: 218547610

Following gene: 218547602

Centisome position: 4.02

GC content: 55.03

Gene sequence:

>756_bases
ATGTTTCCGTTAATGCCGGGATGCGCCAGCAGTTGCAGCGGCAACCAGGTTGATTTCCCGGCCCCGGTTGGCGCACTTAA
TAACACCTGCGACGCACAATCAAGAGCGGTAAGTAATTCAGGTAAGACGGCAGCAACGGGCAACGACGACACAAAACGCT
CCAGAGGGTTAACAATCTTCGCGCCACATTGTAGCATCGCGGTAATGCATAACCGAGTACCTCACATGTCCGGGCCACAA
AAGCTGTTCTTTGCTATCGAATTACCTGCACAAATCCGCAAACAAATCGTTCACTGGCGGGCAGAGCAGTTTCCCCAGGA
AGCGGGCCGCCCGGTTGCCGCCGGGAATCTCCATCTGACGTTGGCATTTTTGGGTGACGTAAGTAGCGAGAAACAGCAGA
TCCTCAGCCAGCTTGCCGGGCGAATTCGTCAGCCCGGTTTCACCCTAAGACTCGACGATGCCGGGCAATGGCCTGGGGCT
CGTGTAATATGGCTGGGAACACGTCAGCCGCCGCGCGAGCTGCTACAACTGGCCAATTTATTGCGCTCACAGGCCGCACG
TAGCGGCTGCTATCAAAGCCCGAAACCTTTTCATCCGCACATTACGCTATTGCGCGACGTAAGCCATGCGGTGGCGATTC
CCGCACCAGGATTTTGCTGGTCATTTCCGGTGAGTGAATTTGTTCTGTATGCCTCCACGTTTACCCGTGGCTCTACCCGC
TATACAGCATTGCAACGCTGGACATTAACGCCATAA

Upstream 100 bases:

>100_bases
CATCCGGTAGCCAACGGTATCACCTGGCTTTTCGTTAAGCAGCTCCGCCAGTCGTTGCGCAACGTTACGCGCCGCCAGAC
GACGCGGCTCCAGCAGGATA

Downstream 100 bases:

>100_bases
CAAGGAATTGATCGATGCAGTTTTTCCCACCTTTACAGCGCGCCACATTAATCCAGCGCTATAAACGCTTTTTAGCCGAT
GTGATCACACCTGATGGTCG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MFPLMPGCASSCSGNQVDFPAPVGALNNTCDAQSRAVSNSGKTAATGNDDTKRSRGLTIFAPHCSIAVMHNRVPHMSGPQ
KLFFAIELPAQIRKQIVHWRAEQFPQEAGRPVAAGNLHLTLAFLGDVSSEKQQILSQLAGRIRQPGFTLRLDDAGQWPGA
RVIWLGTRQPPRELLQLANLLRSQAARSGCYQSPKPFHPHITLLRDVSHAVAIPAPGFCWSFPVSEFVLYASTFTRGSTR
YTALQRWTLTP

Sequences:

>Translated_251_residues
MFPLMPGCASSCSGNQVDFPAPVGALNNTCDAQSRAVSNSGKTAATGNDDTKRSRGLTIFAPHCSIAVMHNRVPHMSGPQ
KLFFAIELPAQIRKQIVHWRAEQFPQEAGRPVAAGNLHLTLAFLGDVSSEKQQILSQLAGRIRQPGFTLRLDDAGQWPGA
RVIWLGTRQPPRELLQLANLLRSQAARSGCYQSPKPFHPHITLLRDVSHAVAIPAPGFCWSFPVSEFVLYASTFTRGSTR
YTALQRWTLTP
>Mature_251_residues
MFPLMPGCASSCSGNQVDFPAPVGALNNTCDAQSRAVSNSGKTAATGNDDTKRSRGLTIFAPHCSIAVMHNRVPHMSGPQ
KLFFAIELPAQIRKQIVHWRAEQFPQEAGRPVAAGNLHLTLAFLGDVSSEKQQILSQLAGRIRQPGFTLRLDDAGQWPGA
RVIWLGTRQPPRELLQLANLLRSQAARSGCYQSPKPFHPHITLLRDVSHAVAIPAPGFCWSFPVSEFVLYASTFTRGSTR
YTALQRWTLTP

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=175, Percent_Identity=75.4285714285714, Blast_Score=279, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 27496; Mature: 27496

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPLMPGCASSCSGNQVDFPAPVGALNNTCDAQSRAVSNSGKTAATGNDDTKRSRGLTIF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHCCCEEE
APHCSIAVMHNRVPHMSGPQKLFFAIELPAQIRKQIVHWRAEQFPQEAGRPVAAGNLHLT
ECCCEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEECCEEEE
LAFLGDVSSEKQQILSQLAGRIRQPGFTLRLDDAGQWPGARVIWLGTRQPPRELLQLANL
EEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHH
LRSQAARSGCYQSPKPFHPHITLLRDVSHAVAIPAPGFCWSFPVSEFVLYASTFTRGSTR
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEECCCCCCEECCCHHHHHHHHHHHCCCCCE
YTALQRWTLTP
EEEEEEEECCC
>Mature Secondary Structure
MFPLMPGCASSCSGNQVDFPAPVGALNNTCDAQSRAVSNSGKTAATGNDDTKRSRGLTIF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHCCCEEE
APHCSIAVMHNRVPHMSGPQKLFFAIELPAQIRKQIVHWRAEQFPQEAGRPVAAGNLHLT
ECCCEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEECCEEEE
LAFLGDVSSEKQQILSQLAGRIRQPGFTLRLDDAGQWPGARVIWLGTRQPPRELLQLANL
EEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHH
LRSQAARSGCYQSPKPFHPHITLLRDVSHAVAIPAPGFCWSFPVSEFVLYASTFTRGSTR
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEECCCCCCEECCCHHHHHHHHHHHCCCCCE
YTALQRWTLTP
EEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]