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The map label for this gene is hofB [H]

Identifier: 218547562

GI number: 218547562

Start: 137569

End: 138954

Strand: Reverse

Name: hofB [H]

Synonym: EFER_0127

Alternate gene names: 218547562

Gene position: 138954-137569 (Counterclockwise)

Preceding gene: 218547563

Following gene: 218547561

Centisome position: 3.03

GC content: 50.29

Gene sequence:

>1386_bases
ATGAATAACGCACAACTTACTGCGCTCTGCCAACGTTACCAGGGCATCTTGTTAGATAGTGATCAAGAAGTGATTAACGT
TGCCGTTGTGGATGCCCCTTCCCATGAGTTGCTGGACGCTCTGCATTTTGCAACACAAAAACGGATCAACATTGTCTGTT
GGACACGCCAACAAATGGAAAGCCACCAGCATCAACCGCAGCAAACACTTCCTGCCGTGGTTTCGCCTGGTCATCTGACG
GCGGCAGAGCTACTCAATCAAACATTGCATTCGGCACTGGCTAAACGAGCTTCTGACATTCATGTCGAACCAGCGGAAAG
TTGTTACCGTATTCGACTACGTATAGATGGCGTTCTTCACGCGCTTCCTGATGTCGCAAAAGAGACGGGAACCGCATTGA
CAGCAAGACTGAAAGTTCTGGGTAATCTCGATATCGCGGAACACCGCCTGCCGCAAGATGGCCAATTTACTGTTGATTTG
GCGGGGAATGCTGTCTCTTTTCGAATTGCTACTCTGCCTTGTCGTGGCGGTGAAAAAGTGGTGTTACGCCTTCTGCATCA
GGTCGAACAAGCACTGGATATTCACCAACTGGGGATGCAACAAAACCAGCTGGAGCCCTTTATTCAGGCACTGCAACAAC
CTCAGGGGCTGATACTGGTGACCGGCCCAACGGGTAGCGGTAAAACTGTCACGTTGTATAGCGCACTGCAAACGCGAAAT
ACGCCACAAAGCAATCTCTGTAGCGTGGAAGATCCTGTTGAGATCCCCATTGACGGATTAAATCAGACACAAATTCACCC
ACGCGCCGGGCTAACCTTTCAGGGAGTATTACGGGCACTTCTTCGCCAGGATCCTGATGTCATCATGGTGGGGGAAATTC
GTGATGGTGAAACAGCAGAAATAGCCATTAAAGCTGCGCAAACCGGGCATCTGGTGCTTTCAACACTGCATACCAACTCC
ACCAGTGAAACTTTGGTGCGGTTGCAACAAATGGGCGTAGCCCACTGGATGTTGTCTTCAGCACTGACACTGGTTGTGGC
CCAACGCCTGGTCAGAAAACTTTGTCCGCATTGCCGTCATATTCATGGCGAGTCAATCACACTTCCTGAGAATTTATGGT
CAACACCACTTCCTCGTTGGCAGGCTCTGGGTTGCAAACACTGCAATCAGGGATTCTACGGTCGAGTTGCGCTGTTTGAA
GTTCTTCCCATCACACCATCGTTACGCCAGTTGATCGCTAACGGTAGTGGTGTGGCAGAACTGGAAACACACGCACAACA
GGAAGGTATCCAGCCGCTTTTTGCTAATGGCTGCCAGGCTGTTGAGCAAGGGTTAACCACGTTTGAAGAGCTCATTCGCG
TGTTGGGAATACCTCATGGCCACTAA

Upstream 100 bases:

>100_bases
CATCACTGGCTGGACACGTAACTGCAATATCCAAAGCGATAGCGCCTTACAACAGGCGTGTGAAGATGTTTTCCGTTTTA
ACGGCAATTAAGGAGCGGCT

Downstream 100 bases:

>100_bases
GCAACTCTGGTGCTGGCAAGGGATTGACAGCGATGGCCACGCACAAGAAGGCGTATTGTGGGCACAAAACCGTGCCGGAC
TGCTGCTTAATTTGCAGCAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNNAQLTALCQRYQGILLDSDQEVINVAVVDAPSHELLDALHFATQKRINIVCWTRQQMESHQHQPQQTLPAVVSPGHLT
AAELLNQTLHSALAKRASDIHVEPAESCYRIRLRIDGVLHALPDVAKETGTALTARLKVLGNLDIAEHRLPQDGQFTVDL
AGNAVSFRIATLPCRGGEKVVLRLLHQVEQALDIHQLGMQQNQLEPFIQALQQPQGLILVTGPTGSGKTVTLYSALQTRN
TPQSNLCSVEDPVEIPIDGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMVGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TSETLVRLQQMGVAHWMLSSALTLVVAQRLVRKLCPHCRHIHGESITLPENLWSTPLPRWQALGCKHCNQGFYGRVALFE
VLPITPSLRQLIANGSGVAELETHAQQEGIQPLFANGCQAVEQGLTTFEELIRVLGIPHGH

Sequences:

>Translated_461_residues
MNNAQLTALCQRYQGILLDSDQEVINVAVVDAPSHELLDALHFATQKRINIVCWTRQQMESHQHQPQQTLPAVVSPGHLT
AAELLNQTLHSALAKRASDIHVEPAESCYRIRLRIDGVLHALPDVAKETGTALTARLKVLGNLDIAEHRLPQDGQFTVDL
AGNAVSFRIATLPCRGGEKVVLRLLHQVEQALDIHQLGMQQNQLEPFIQALQQPQGLILVTGPTGSGKTVTLYSALQTRN
TPQSNLCSVEDPVEIPIDGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMVGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TSETLVRLQQMGVAHWMLSSALTLVVAQRLVRKLCPHCRHIHGESITLPENLWSTPLPRWQALGCKHCNQGFYGRVALFE
VLPITPSLRQLIANGSGVAELETHAQQEGIQPLFANGCQAVEQGLTTFEELIRVLGIPHGH
>Mature_461_residues
MNNAQLTALCQRYQGILLDSDQEVINVAVVDAPSHELLDALHFATQKRINIVCWTRQQMESHQHQPQQTLPAVVSPGHLT
AAELLNQTLHSALAKRASDIHVEPAESCYRIRLRIDGVLHALPDVAKETGTALTARLKVLGNLDIAEHRLPQDGQFTVDL
AGNAVSFRIATLPCRGGEKVVLRLLHQVEQALDIHQLGMQQNQLEPFIQALQQPQGLILVTGPTGSGKTVTLYSALQTRN
TPQSNLCSVEDPVEIPIDGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMVGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TSETLVRLQQMGVAHWMLSSALTLVVAQRLVRKLCPHCRHIHGESITLPENLWSTPLPRWQALGCKHCNQGFYGRVALFE
VLPITPSLRQLIANGSGVAELETHAQQEGIQPLFANGCQAVEQGLTTFEELIRVLGIPHGH

Specific function: Unknown

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=460, Percent_Identity=78.695652173913, Blast_Score=730, Evalue=0.0,
Organism=Escherichia coli, GI1789723, Length=388, Percent_Identity=44.3298969072165, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI87082188, Length=335, Percent_Identity=31.9402985074627, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 50546; Mature: 50546

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNAQLTALCQRYQGILLDSDQEVINVAVVDAPSHELLDALHFATQKRINIVCWTRQQME
CCCCHHHHHHHHHCCCEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECHHHHH
SHQHQPQQTLPAVVSPGHLTAAELLNQTLHSALAKRASDIHVEPAESCYRIRLRIDGVLH
HHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHH
ALPDVAKETGTALTARLKVLGNLDIAEHRLPQDGQFTVDLAGNAVSFRIATLPCRGGEKV
HHHHHHHHHCHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECCCEEEEEEEEECCCCCHHH
VLRLLHQVEQALDIHQLGMQQNQLEPFIQALQQPQGLILVTGPTGSGKTVTLYSALQTRN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEHHHHCC
TPQSNLCSVEDPVEIPIDGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMVGEIRDGETAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEE
IAIKAAQTGHLVLSTLHTNSTSETLVRLQQMGVAHWMLSSALTLVVAQRLVRKLCPHCRH
EEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
IHGESITLPENLWSTPLPRWQALGCKHCNQGFYGRVALFEVLPITPSLRQLIANGSGVAE
CCCCCEECCHHHCCCCCCHHHHCCHHHCCCCCCCCEEEHEEECCCHHHHHHHHCCCCCHH
LETHAQQEGIQPLFANGCQAVEQGLTTFEELIRVLGIPHGH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNNAQLTALCQRYQGILLDSDQEVINVAVVDAPSHELLDALHFATQKRINIVCWTRQQME
CCCCHHHHHHHHHCCCEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECHHHHH
SHQHQPQQTLPAVVSPGHLTAAELLNQTLHSALAKRASDIHVEPAESCYRIRLRIDGVLH
HHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHH
ALPDVAKETGTALTARLKVLGNLDIAEHRLPQDGQFTVDLAGNAVSFRIATLPCRGGEKV
HHHHHHHHHCHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECCCEEEEEEEEECCCCCHHH
VLRLLHQVEQALDIHQLGMQQNQLEPFIQALQQPQGLILVTGPTGSGKTVTLYSALQTRN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEHHHHCC
TPQSNLCSVEDPVEIPIDGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMVGEIRDGETAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEE
IAIKAAQTGHLVLSTLHTNSTSETLVRLQQMGVAHWMLSSALTLVVAQRLVRKLCPHCRH
EEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
IHGESITLPENLWSTPLPRWQALGCKHCNQGFYGRVALFEVLPITPSLRQLIANGSGVAE
CCCCCEECCHHHCCCCCCHHHHCCHHHCCCCCCCCEEEHEEECCCHHHHHHHHCCCCCHH
LETHAQQEGIQPLFANGCQAVEQGLTTFEELIRVLGIPHGH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959070; 8202364; 9278503 [H]