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The map label for this gene is tnpR [H]

Identifier: 218533242

GI number: 218533242

Start: 5761142

End: 5761774

Strand: Reverse

Name: tnpR [H]

Synonym: Mchl_5372

Alternate gene names: 218533242

Gene position: 5761774-5761142 (Counterclockwise)

Preceding gene: 218533246

Following gene: 218533241

Centisome position: 99.72

GC content: 66.82

Gene sequence:

>633_bases
ATGAAGACGATTCTCTACGTCCGCGTCAGCACCACCGACCAGACCAGCGCGCACCAGCGCAAGCAGGCCGAGGGAGCAGG
ATTCAAGGTGGACGACGTTTTAGCTGATGACGGCGTGTCGGGTGTCACGACCCTCCTGGCCGAGCGCTCGCAGGGCAAGC
GCCTGTTCGACATGCTCCGGCGCGGTGACACGCTCGTGGTCCGTTGGGTGGACCGTCTGGGCCGCAACTATGCCGACGTG
ACTGAGACGATCCGCGAGTTCATGCGCCGGGGCGTCATCGTCCGGACGGTCATCAACAACATGACCTTCGACGGCGCGAC
CACGGACCCGGTGCAGATGGCCGTCCGCGATGCCCTTATCGGCTTCATGGCCGCGACTGCCCAGGCTCAGGCCGAGGCGA
CGAAGGAGGCACAGAAGGCCGGCATCGCGCACGCCAAGGATCGGGAGGAAGCCTACCGGGGCCGGAAGCCCAGCTACTCG
CGCGCCCAGCTCGACGCCGTTCGCGCTCAGCTCGCTCAGGGTGCCAGCGTATCAGCCATCGCCCGAGACACCGGCCTGTC
GAGACAGACCGTCTACCGCATTCAAGAAGACCCGGCCGAAGCCGAGGCCGCGCTTGCCCGATGGGCCGCGTGA

Upstream 100 bases:

>100_bases
GACAGCGGGAATCCGTCCCAAAGTGTGTCCGTAAGGGTTGACCTGTCCATTCGGACAAAGCTATATACGGACAGCACCAC
AGCGGACACGGCGAACACCG

Downstream 100 bases:

>100_bases
GAAGGAGACCATGACCGTGACCGCCCCGTCAGCGAAGCGCCGCTACGTGAAGCTCACCGAGGCCGAATGGGCAGAGGCCG
AGGCCGCATGGGCTTCCGGC

Product: Resolvase domain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKTILYVRVSTTDQTSAHQRKQAEGAGFKVDDVLADDGVSGVTTLLAERSQGKRLFDMLRRGDTLVVRWVDRLGRNYADV
TETIREFMRRGVIVRTVINNMTFDGATTDPVQMAVRDALIGFMAATAQAQAEATKEAQKAGIAHAKDREEAYRGRKPSYS
RAQLDAVRAQLAQGASVSAIARDTGLSRQTVYRIQEDPAEAEAALARWAA

Sequences:

>Translated_210_residues
MKTILYVRVSTTDQTSAHQRKQAEGAGFKVDDVLADDGVSGVTTLLAERSQGKRLFDMLRRGDTLVVRWVDRLGRNYADV
TETIREFMRRGVIVRTVINNMTFDGATTDPVQMAVRDALIGFMAATAQAQAEATKEAQKAGIAHAKDREEAYRGRKPSYS
RAQLDAVRAQLAQGASVSAIARDTGLSRQTVYRIQEDPAEAEAALARWAA
>Mature_210_residues
MKTILYVRVSTTDQTSAHQRKQAEGAGFKVDDVLADDGVSGVTTLLAERSQGKRLFDMLRRGDTLVVRWVDRLGRNYADV
TETIREFMRRGVIVRTVINNMTFDGATTDPVQMAVRDALIGFMAATAQAQAEATKEAQKAGIAHAKDREEAYRGRKPSYS
RAQLDAVRAQLAQGASVSAIARDTGLSRQTVYRIQEDPAEAEAALARWAA

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787827, Length=189, Percent_Identity=32.2751322751323, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI1787638, Length=189, Percent_Identity=31.7460317460317, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1787404, Length=192, Percent_Identity=31.25, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 23061; Mature: 23061

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTILYVRVSTTDQTSAHQRKQAEGAGFKVDDVLADDGVSGVTTLLAERSQGKRLFDMLR
CCEEEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHH
RGDTLVVRWVDRLGRNYADVTETIREFMRRGVIVRTVINNMTFDGATTDPVQMAVRDALI
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
GFMAATAQAQAEATKEAQKAGIAHAKDREEAYRGRKPSYSRAQLDAVRAQLAQGASVSAI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHH
ARDTGLSRQTVYRIQEDPAEAEAALARWAA
HHHCCCCHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTILYVRVSTTDQTSAHQRKQAEGAGFKVDDVLADDGVSGVTTLLAERSQGKRLFDMLR
CCEEEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHH
RGDTLVVRWVDRLGRNYADVTETIREFMRRGVIVRTVINNMTFDGATTDPVQMAVRDALI
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
GFMAATAQAQAEATKEAQKAGIAHAKDREEAYRGRKPSYSRAQLDAVRAQLAQGASVSAI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHH
ARDTGLSRQTVYRIQEDPAEAEAALARWAA
HHHCCCCHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2992807; 6312271; 3029727 [H]