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The map label for this gene is rpoE [H]

Identifier: 218533227

GI number: 218533227

Start: 5741256

End: 5742002

Strand: Reverse

Name: rpoE [H]

Synonym: Mchl_5355

Alternate gene names: 218533227

Gene position: 5742002-5741256 (Counterclockwise)

Preceding gene: 218533230

Following gene: 218533222

Centisome position: 99.38

GC content: 66.53

Gene sequence:

>747_bases
ATGCCAAAGACGACCCTCGACGACCTCGCCTTGCACCCCGATGACGAGGTGCCGACGCTCCCTGCTGCTGTCCAGCAACG
CCTCGGAGCGCTTCTCGCCGATGCCTATGCGCAGATGCCGGGGAATCCGGGTGTCGAAGCCGGTCGCTTCGACGCCTTGC
TGGCCAGGCTGGAGACCGTCCTCGTCGCCCAGGAAGGGAAGGACGAGGAATCGTTCCGGGCCGCCATCATCGAGATGACG
CCGCGCCTCTACAACTTCGCCATGTCGCTGACGAAGAACCCGTCGGCCGCCGACGACCTCGTGCAGGATACGTTCCTGCG
GGCGTGGCGCAGCCGTGCGCGTTTCACCGTCGGCACGAATCTCGGCGCGTGGCTGTTCACGATCATGCGCAACGCCTTCT
ACTCGCGCCACCGCAAGGAGGTGCGCGAGGTCGCCGACAGCGACGGCGATTACGCCGAGCGCCTCGCAGCAGCACCCGAG
CAATCGGGCCATGTTGATCTGATGGATGCGCAGACCGCGCTCGCCAAGCTTCCCCTGCCGATGCGGCAGGCGTTGATCCT
CGTGGCGATCGAGAACCTCAGCTACGAGGAGGCGGCGACCGTCATGAACTGCCGCATTGGCACGGTGAAGAGCCGGGTCT
GGCGTGCCCGGGAGCAACTCGCGCAGATCCTCGGCTACAGCGCCGCCGATGTCGGCTCCGACAGCATGACGCTGTCCGCC
GTCAGCGGCTCGACCTGGATGGCCTGA

Upstream 100 bases:

>100_bases
CCTGATAATCTATGTTGGCGCAGGGGACCGCAAATCGCGAGACAAGTCCGGCGTTGGGGCACGAACCCACGCGCGCGTCC
GTGACCAGCCACAGGACCTG

Downstream 100 bases:

>100_bases
AGCGCGGCCCGACGAAGCGGCCACCGCTTCGTCAAAGAAACGCGTCAATATAAGGACCGAGGCGTGCCAACCTGATGCCA
ATCAGGTCGGATAAGGCTTT

Product: RNA polymerase, sigma-24 subunit, ECF subfamily

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MPKTTLDDLALHPDDEVPTLPAAVQQRLGALLADAYAQMPGNPGVEAGRFDALLARLETVLVAQEGKDEESFRAAIIEMT
PRLYNFAMSLTKNPSAADDLVQDTFLRAWRSRARFTVGTNLGAWLFTIMRNAFYSRHRKEVREVADSDGDYAERLAAAPE
QSGHVDLMDAQTALAKLPLPMRQALILVAIENLSYEEAATVMNCRIGTVKSRVWRAREQLAQILGYSAADVGSDSMTLSA
VSGSTWMA

Sequences:

>Translated_248_residues
MPKTTLDDLALHPDDEVPTLPAAVQQRLGALLADAYAQMPGNPGVEAGRFDALLARLETVLVAQEGKDEESFRAAIIEMT
PRLYNFAMSLTKNPSAADDLVQDTFLRAWRSRARFTVGTNLGAWLFTIMRNAFYSRHRKEVREVADSDGDYAERLAAAPE
QSGHVDLMDAQTALAKLPLPMRQALILVAIENLSYEEAATVMNCRIGTVKSRVWRAREQLAQILGYSAADVGSDSMTLSA
VSGSTWMA
>Mature_247_residues
PKTTLDDLALHPDDEVPTLPAAVQQRLGALLADAYAQMPGNPGVEAGRFDALLARLETVLVAQEGKDEESFRAAIIEMTP
RLYNFAMSLTKNPSAADDLVQDTFLRAWRSRARFTVGTNLGAWLFTIMRNAFYSRHRKEVREVADSDGDYAERLAAAPEQ
SGHVDLMDAQTALAKLPLPMRQALILVAIENLSYEEAATVMNCRIGTVKSRVWRAREQLAQILGYSAADVGSDSMTLSAV
SGSTWMA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=134, Percent_Identity=32.089552238806, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 27189; Mature: 27058

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTTLDDLALHPDDEVPTLPAAVQQRLGALLADAYAQMPGNPGVEAGRFDALLARLETV
CCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LVAQEGKDEESFRAAIIEMTPRLYNFAMSLTKNPSAADDLVQDTFLRAWRSRARFTVGTN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEECCC
LGAWLFTIMRNAFYSRHRKEVREVADSDGDYAERLAAAPEQSGHVDLMDAQTALAKLPLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCH
MRQALILVAIENLSYEEAATVMNCRIGTVKSRVWRAREQLAQILGYSAADVGSDSMTLSA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEE
VSGSTWMA
ECCCCCCC
>Mature Secondary Structure 
PKTTLDDLALHPDDEVPTLPAAVQQRLGALLADAYAQMPGNPGVEAGRFDALLARLETV
CCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LVAQEGKDEESFRAAIIEMTPRLYNFAMSLTKNPSAADDLVQDTFLRAWRSRARFTVGTN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEECCC
LGAWLFTIMRNAFYSRHRKEVREVADSDGDYAERLAAAPEQSGHVDLMDAQTALAKLPLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCH
MRQALILVAIENLSYEEAATVMNCRIGTVKSRVWRAREQLAQILGYSAADVGSDSMTLSA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEE
VSGSTWMA
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]