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The map label for this gene is livK [C]

Identifier: 218533212

GI number: 218533212

Start: 5723344

End: 5724534

Strand: Reverse

Name: livK [C]

Synonym: Mchl_5340

Alternate gene names: 218533212

Gene position: 5724534-5723344 (Counterclockwise)

Preceding gene: 218533214

Following gene: 218533211

Centisome position: 99.08

GC content: 68.35

Gene sequence:

>1191_bases
ATGCCCCAACGCCTCAAGCCCACCCGCTCCCTCGCACGGCGTTTCGTCCTCGGCGCCCTTTTGGCGGGGGTTACCAGCAT
CGGTGCCGTCCGGGCCGCAGAGCCGGTGAAGATCGGTCTCGTCACCGCCCTCAGCGGCCAATCTGCGAAATCGGGCGAGG
CGATCTCCCGCGGGATTGGGCTGGCGATCGAAGAGATCAACCGCAACGGCGGCGTCCTCGGCCGCCCGCTGGAACTCGTC
GCCCGCGACGACGAGGCCAACCCGTCCAAGGGCGTGCTCGCCGCCCGAGAGCTGATCCAGCGGGCCGGCGTGGTCGCGCT
GATCGGCGGTCTCGACACGCCGGTCTCGATGGCAATCGTGCCGATCGCCAACCAGATGAAGGTGCCCTTCGTCGGCCCTT
GGGCGGCCGGCACCGGCATCACCCGCAACGGCGCGGTCGACAACTATGTCTTCCGGGTTTCCGCGGTCGATGCGCTCGTG
GACGAGGCCCTCGTGGCCTACGCGGTCAAGACCTACGGCGCCAAGAAACCCGGCGCGATCCTCGTCAACAACGCCTGGGG
CGAGTCGAACCAGCAGGGGCTGGTCTCGGCGCTGAAGGCCGCGGGTCTCCCCAGCGCCGGCATCGAAAAGTACGAGGCCA
ACGACATCGACATGGTCCCGCAACTCTCCCGCCTGCGGGAGGCCGGGGCCGACGCGCTGTTCCTCGTCGGCAATGTCGGC
CCCTCGGCCCAGGTGGTGAAGTCCCTCGATCGGATGGGCTGGACCGTGCCGGTGATCTCCCATTGGGGACCGGCGGGCGG
GCGCTTCGACGAACTCGCTGGGCCGGGTGCGGCGCGGGTGCACTTCGTCCAGACCTTCACCTTCGCGGGCAACGACAGCC
CGAAGGCGAAGGCGGTACTCGACGCGCTGAAGGCGAAGTACCCGGCGATCAAGTCCATGGCTGACGTCACGCCCGCCGTC
GGCATCGCCAACGCCTACGACGCCACGCACCTGATCGCCCTGGCGATCCAGGCAGCGGGCGCCACCGAGGGGCCGAAGGT
TCGCGACGGCTTCTACAAGGTCGCGCCCTATTCCGGCCTGATCAAGACCTACGAAGCGCCGTTCGCACCGGGCCAGCACG
ACGCCTTGAAGCCGGACGACTACATCTTCGCAAGCTTCAAGAACGGCGAGATCGTCGCGGTGTCCAAGTGA

Upstream 100 bases:

>100_bases
GCGGCGGGCTCCGGGCGGGAAAATGCGAGAAGAAACAAGCACGTTTCATCTGGCACGCCCGTTGCGGTCATTGGTCGACC
GCATACGGAGCCGGATCGCC

Downstream 100 bases:

>100_bases
ACGGCGTCCGGAGCCGTCTTCCCGGAAGAGGGCAGGGGATGATGTCACCATGCTGACCAGCACCCTCGTCAGCGGGCTCG
GCCTCGGCAGCATGTACGGG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MPQRLKPTRSLARRFVLGALLAGVTSIGAVRAAEPVKIGLVTALSGQSAKSGEAISRGIGLAIEEINRNGGVLGRPLELV
ARDDEANPSKGVLAARELIQRAGVVALIGGLDTPVSMAIVPIANQMKVPFVGPWAAGTGITRNGAVDNYVFRVSAVDALV
DEALVAYAVKTYGAKKPGAILVNNAWGESNQQGLVSALKAAGLPSAGIEKYEANDIDMVPQLSRLREAGADALFLVGNVG
PSAQVVKSLDRMGWTVPVISHWGPAGGRFDELAGPGAARVHFVQTFTFAGNDSPKAKAVLDALKAKYPAIKSMADVTPAV
GIANAYDATHLIALAIQAAGATEGPKVRDGFYKVAPYSGLIKTYEAPFAPGQHDALKPDDYIFASFKNGEIVAVSK

Sequences:

>Translated_396_residues
MPQRLKPTRSLARRFVLGALLAGVTSIGAVRAAEPVKIGLVTALSGQSAKSGEAISRGIGLAIEEINRNGGVLGRPLELV
ARDDEANPSKGVLAARELIQRAGVVALIGGLDTPVSMAIVPIANQMKVPFVGPWAAGTGITRNGAVDNYVFRVSAVDALV
DEALVAYAVKTYGAKKPGAILVNNAWGESNQQGLVSALKAAGLPSAGIEKYEANDIDMVPQLSRLREAGADALFLVGNVG
PSAQVVKSLDRMGWTVPVISHWGPAGGRFDELAGPGAARVHFVQTFTFAGNDSPKAKAVLDALKAKYPAIKSMADVTPAV
GIANAYDATHLIALAIQAAGATEGPKVRDGFYKVAPYSGLIKTYEAPFAPGQHDALKPDDYIFASFKNGEIVAVSK
>Mature_395_residues
PQRLKPTRSLARRFVLGALLAGVTSIGAVRAAEPVKIGLVTALSGQSAKSGEAISRGIGLAIEEINRNGGVLGRPLELVA
RDDEANPSKGVLAARELIQRAGVVALIGGLDTPVSMAIVPIANQMKVPFVGPWAAGTGITRNGAVDNYVFRVSAVDALVD
EALVAYAVKTYGAKKPGAILVNNAWGESNQQGLVSALKAAGLPSAGIEKYEANDIDMVPQLSRLREAGADALFLVGNVGP
SAQVVKSLDRMGWTVPVISHWGPAGGRFDELAGPGAARVHFVQTFTFAGNDSPKAKAVLDALKAKYPAIKSMADVTPAVG
IANAYDATHLIALAIQAAGATEGPKVRDGFYKVAPYSGLIKTYEAPFAPGQHDALKPDDYIFASFKNGEIVAVSK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=328, Percent_Identity=22.8658536585366, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994941, Length=290, Percent_Identity=22.0689655172414, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41154; Mature: 41023

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQRLKPTRSLARRFVLGALLAGVTSIGAVRAAEPVKIGLVTALSGQSAKSGEAISRGIG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHCCC
LAIEEINRNGGVLGRPLELVARDDEANPSKGVLAARELIQRAGVVALIGGLDTPVSMAIV
CEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCEEEEE
PIANQMKVPFVGPWAAGTGITRNGAVDNYVFRVSAVDALVDEALVAYAVKTYGAKKPGAI
ECCCCCCCCEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LVNNAWGESNQQGLVSALKAAGLPSAGIEKYEANDIDMVPQLSRLREAGADALFLVGNVG
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
PSAQVVKSLDRMGWTVPVISHWGPAGGRFDELAGPGAARVHFVQTFTFAGNDSPKAKAVL
CHHHHHHHHHHCCCEEEEHHCCCCCCCCCHHHCCCCCCEEEEEEEEEECCCCCCHHHHHH
DALKAKYPAIKSMADVTPAVGIANAYDATHLIALAIQAAGATEGPKVRDGFYKVAPYSGL
HHHHHHCHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHH
IKTYEAPFAPGQHDALKPDDYIFASFKNGEIVAVSK
HHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEC
>Mature Secondary Structure 
PQRLKPTRSLARRFVLGALLAGVTSIGAVRAAEPVKIGLVTALSGQSAKSGEAISRGIG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHCCC
LAIEEINRNGGVLGRPLELVARDDEANPSKGVLAARELIQRAGVVALIGGLDTPVSMAIV
CEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCEEEEE
PIANQMKVPFVGPWAAGTGITRNGAVDNYVFRVSAVDALVDEALVAYAVKTYGAKKPGAI
ECCCCCCCCEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LVNNAWGESNQQGLVSALKAAGLPSAGIEKYEANDIDMVPQLSRLREAGADALFLVGNVG
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
PSAQVVKSLDRMGWTVPVISHWGPAGGRFDELAGPGAARVHFVQTFTFAGNDSPKAKAVL
CHHHHHHHHHHCCCEEEEHHCCCCCCCCCHHHCCCCCCEEEEEEEEEECCCCCCHHHHHH
DALKAKYPAIKSMADVTPAVGIANAYDATHLIALAIQAAGATEGPKVRDGFYKVAPYSGL
HHHHHHCHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHH
IKTYEAPFAPGQHDALKPDDYIFASFKNGEIVAVSK
HHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]