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The map label for this gene is tolB [H]

Identifier: 218533101

GI number: 218533101

Start: 5594172

End: 5595512

Strand: Reverse

Name: tolB [H]

Synonym: Mchl_5227

Alternate gene names: 218533101

Gene position: 5595512-5594172 (Counterclockwise)

Preceding gene: 218533102

Following gene: 218533099

Centisome position: 96.84

GC content: 70.32

Gene sequence:

>1341_bases
ATGCCTGGAACAGCCTTCACCCGGCGCACTCTCCTGCCGGCGCTCACGGCCGCGCTCGGCGTCCTCGCCCTGGCGCTGCC
CGCCGCGACGCCGGCGCGGGCGCAGCTTCAACTCCGCATCGGCAGCGGCGCCTTTCAGCCGATGCCGATCGCCGTCGCCG
ACTTCGCCGGTGACGCCAGCCTCGGCACGCTGGTCGCCAGCGTGATCACGAACAATCTGCGCCGCTCCGGCTACTTCACG
CCGCTGGAGAAGGGGCGCTTCCCCGAGAACCCGAGCTTCGATGCGGCGCCGAACTTCGAGGCGTGGAAGGGCGCGGGCGT
CCAAGCGCTCGTGACCGGCCGGGTCGGGCGCGACGCCTCAGGCCGGCTCACCGTCGCCTTCCGCCTGTGGGACGTGGCCT
CGGGCCAGCAGATCACGGGTCAGCAATACGGCACCGACGTCGCCAACGCGCGCCGCACCGGGCATCTCGTCTCGGACGCG
GTCTACACCAAGGTCACCGGCCTCGGGCCGTGGTTCGACAGCCGCGTCGCCTTTGTCGATGAGTCCGGCCCGAAGGAGAA
CCGCCGCAAGCGCCTGATGGTGATGGATCAGGACGGCGCCAACGTCCGCGCGCTGACCGGGGGCGGCGACGTCTCGGTGG
TGGCGCCGCGCTACTCGCCGGCGGGGCAGGACATCGCCTTCATGACCCAGGCGCCGGGCCAGCAGCCCAAGGTCCAGATG
ATCGATCTGGAGACCGGCAAGCGTCAGACGGTCGGCAACTTCGCCTCGATGAGCGCGAGCCCGCGCTTCTCGCCGGACGG
GCGGCGCCTCGTGATGAGCGTGCAGCAGGGCGGCAACGCCGACATCGTCACCGTCGATATCGCCTCGAAGGCGCAGACGC
CGGTCACGCAAGGTCTCGCGATCGACACCTCGCCGAGCTACGCGCCCGACGGCAGCCAGATCGTGTTCGAATCCGACCGC
GGCGGCTCGCAGCAAATCTATGTGATGGGCGCCGACGGCTCGAACCCGCGCCGGCTCTCCTTCGGCGAAGGCTCGGCCTC
GCAGCCGGCTTGGTCGCCCCGCGGCGACCTCATCGCCTTCACCCGGCAGCGCAAGGGCGGCTTTGCCATCTGTGTGATGA
AGCCCGACGGCTCGGGCGAGCGGGTGCTGACCGAGGGCTTCCACAACGAGAGCCCGACCTTCGCGCCGAACGGCCAGTAC
GTGATGTTCTTCCGTGATCCCGGCGGCCAGGGCGGCGGCAAGCTCTACATGGTCGACATCACCGGCAAGGTGGAGCAGCC
GGTGCCGACCCCGTCCTTCGCCTCGGACCCAACCTGGTCGCCGCTGCTGTCGGGCAAGTAA

Upstream 100 bases:

>100_bases
GTTCGAGTTCTCGCCCGTGTGATCCGCCGGCTCGACCCGCCGATCCACCGCGTCATCGGCCTTTCTGTCTCGCATTCCCT
GAACGACCTGGACTTGTCCC

Downstream 100 bases:

>100_bases
GGCGGCCGGGAGCGCACAGTGCGCGGCCGAAATCTCGCAATGACGGAGCGGCAAACCCACACCGTCGTTGCGAGCCGAAC
GGCTTCGCTTCAACAGCACG

Product: Tol-Pal system beta propeller repeat protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPGTAFTRRTLLPALTAALGVLALALPAATPARAQLQLRIGSGAFQPMPIAVADFAGDASLGTLVASVITNNLRRSGYFT
PLEKGRFPENPSFDAAPNFEAWKGAGVQALVTGRVGRDASGRLTVAFRLWDVASGQQITGQQYGTDVANARRTGHLVSDA
VYTKVTGLGPWFDSRVAFVDESGPKENRRKRLMVMDQDGANVRALTGGGDVSVVAPRYSPAGQDIAFMTQAPGQQPKVQM
IDLETGKRQTVGNFASMSASPRFSPDGRRLVMSVQQGGNADIVTVDIASKAQTPVTQGLAIDTSPSYAPDGSQIVFESDR
GGSQQIYVMGADGSNPRRLSFGEGSASQPAWSPRGDLIAFTRQRKGGFAICVMKPDGSGERVLTEGFHNESPTFAPNGQY
VMFFRDPGGQGGGKLYMVDITGKVEQPVPTPSFASDPTWSPLLSGK

Sequences:

>Translated_446_residues
MPGTAFTRRTLLPALTAALGVLALALPAATPARAQLQLRIGSGAFQPMPIAVADFAGDASLGTLVASVITNNLRRSGYFT
PLEKGRFPENPSFDAAPNFEAWKGAGVQALVTGRVGRDASGRLTVAFRLWDVASGQQITGQQYGTDVANARRTGHLVSDA
VYTKVTGLGPWFDSRVAFVDESGPKENRRKRLMVMDQDGANVRALTGGGDVSVVAPRYSPAGQDIAFMTQAPGQQPKVQM
IDLETGKRQTVGNFASMSASPRFSPDGRRLVMSVQQGGNADIVTVDIASKAQTPVTQGLAIDTSPSYAPDGSQIVFESDR
GGSQQIYVMGADGSNPRRLSFGEGSASQPAWSPRGDLIAFTRQRKGGFAICVMKPDGSGERVLTEGFHNESPTFAPNGQY
VMFFRDPGGQGGGKLYMVDITGKVEQPVPTPSFASDPTWSPLLSGK
>Mature_445_residues
PGTAFTRRTLLPALTAALGVLALALPAATPARAQLQLRIGSGAFQPMPIAVADFAGDASLGTLVASVITNNLRRSGYFTP
LEKGRFPENPSFDAAPNFEAWKGAGVQALVTGRVGRDASGRLTVAFRLWDVASGQQITGQQYGTDVANARRTGHLVSDAV
YTKVTGLGPWFDSRVAFVDESGPKENRRKRLMVMDQDGANVRALTGGGDVSVVAPRYSPAGQDIAFMTQAPGQQPKVQMI
DLETGKRQTVGNFASMSASPRFSPDGRRLVMSVQQGGNADIVTVDIASKAQTPVTQGLAIDTSPSYAPDGSQIVFESDRG
GSQQIYVMGADGSNPRRLSFGEGSASQPAWSPRGDLIAFTRQRKGGFAICVMKPDGSGERVLTEGFHNESPTFAPNGQYV
MFFRDPGGQGGGKLYMVDITGKVEQPVPTPSFASDPTWSPLLSGK

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=440, Percent_Identity=30.4545454545455, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR006311
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 47192; Mature: 47061

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTAFTRRTLLPALTAALGVLALALPAATPARAQLQLRIGSGAFQPMPIAVADFAGDAS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCH
LGTLVASVITNNLRRSGYFTPLEKGRFPENPSFDAAPNFEAWKGAGVQALVTGRVGRDAS
HHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCHHHCCCCEEEEEECCCCCCCC
GRLTVAFRLWDVASGQQITGQQYGTDVANARRTGHLVSDAVYTKVTGLGPWFDSRVAFVD
CEEEEEEEEEECCCCCEECCHHCCCHHHHHHHCCCHHHHHHHHEECCCCCCCCCEEEEEE
ESGPKENRRKRLMVMDQDGANVRALTGGGDVSVVAPRYSPAGQDIAFMTQAPGQQPKVQM
CCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEECCCCCCCCCEEEEECCCCCCCCEEE
IDLETGKRQTVGNFASMSASPRFSPDGRRLVMSVQQGGNADIVTVDIASKAQTPVTQGLA
EEECCCCCCCCCCHHHCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCEE
IDTSPSYAPDGSQIVFESDRGGSQQIYVMGADGSNPRRLSFGEGSASQPAWSPRGDLIAF
ECCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEE
TRQRKGGFAICVMKPDGSGERVLTEGFHNESPTFAPNGQYVMFFRDPGGQGGGKLYMVDI
EEECCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
TGKVEQPVPTPSFASDPTWSPLLSGK
CCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PGTAFTRRTLLPALTAALGVLALALPAATPARAQLQLRIGSGAFQPMPIAVADFAGDAS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCH
LGTLVASVITNNLRRSGYFTPLEKGRFPENPSFDAAPNFEAWKGAGVQALVTGRVGRDAS
HHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCHHHCCCCEEEEEECCCCCCCC
GRLTVAFRLWDVASGQQITGQQYGTDVANARRTGHLVSDAVYTKVTGLGPWFDSRVAFVD
CEEEEEEEEEECCCCCEECCHHCCCHHHHHHHCCCHHHHHHHHEECCCCCCCCCEEEEEE
ESGPKENRRKRLMVMDQDGANVRALTGGGDVSVVAPRYSPAGQDIAFMTQAPGQQPKVQM
CCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEECCCCCCCCCEEEEECCCCCCCCEEE
IDLETGKRQTVGNFASMSASPRFSPDGRRLVMSVQQGGNADIVTVDIASKAQTPVTQGLA
EEECCCCCCCCCCHHHCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCEE
IDTSPSYAPDGSQIVFESDRGGSQQIYVMGADGSNPRRLSFGEGSASQPAWSPRGDLIAF
ECCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEE
TRQRKGGFAICVMKPDGSGERVLTEGFHNESPTFAPNGQYVMFFRDPGGQGGGKLYMVDI
EEECCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
TGKVEQPVPTPSFASDPTWSPLLSGK
CCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA