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The map label for this gene is dmlA [H]

Identifier: 218533028

GI number: 218533028

Start: 5518679

End: 5519758

Strand: Reverse

Name: dmlA [H]

Synonym: Mchl_5152

Alternate gene names: 218533028

Gene position: 5519758-5518679 (Counterclockwise)

Preceding gene: 218533029

Following gene: 218533027

Centisome position: 95.53

GC content: 67.41

Gene sequence:

>1080_bases
ATGGGCGGGACGAAGCGCAGCTACCGGATCGCGGTGATCCCCGGCGACGGCATCGGCAAGGAAGTCATGCCGGAGGGCGT
TCGGGTTCTGGAGCGGGCGGCCGCCCGCCACGGCTTCGAGATCGTGCAGGACTGGTTCGATTTCGCGAGCTGCGACTACT
ACGAGGCGCACGGGCGGATGATGCCCGAGGACTGGAAGGCGCAGATCGGCTCCCACGATGCGATCTTCTTCGGCGCCGTC
GGCATGCCCGAGCGCGTGCCCGACCACATCTCGCTCTGGGGCTCGCTGATCCAGTTTCGGCGCGAGTTCGACCAATACGT
CAACCTGCGCCCTGTGCGCCTGATGCCGGGCGTGCCGTCACCGCTCGCCGGCCGCAAGCCCGGCGACATCGACTTCTGGG
TCGTCCGCGAGAACACCGAAGGTGAGTATTCCAACGCGGGCGGGCGGATGTTCGCCGGCACCGAGCGCGAGTTCGCGGTT
CAGGAATCGGTGTTCACCCGCCACGGCGTCGATCGCGTGCTGAAGTTCGCCTTCGAATTGGCGCAGAGCCGGCCCAAGCG
TCACCTGACCTCGGCGACTAAATCGAACGGCATCTCGATCACCATGCCGTTCTGGGACGAGCGGGTGCGGGACGTGGCCG
AGGGCTACCCTGAGATCCGCTGGGACCAGTACCACATCGACATCCTGACCGCGCATTTCGTGCTGAACCCGGACCGGTTC
GACGTGGTGGTGGCCTCGAACCTCTTCGGCGACATTCTCTCCGATCTCGGGCCCGCCTGCACCGGCACCATCGGCATCGC
GCCTTCGGGCAACATCAATCCCGAGCAGCTCTATCCGTCCGTGTTCGAACCGGTGCACGGCTCGGCCCCCGACATCGCCG
GACAGGGAATCGCCAACCCAATCGGCCAGATCTGGTCGGGGGCGATGATGCTGGAGCATCTCGGCGAGCGGGAGGCCGCG
GCCGAGATCGTCGCTGGCATCGAGCGGGTGCTCACTGAGCGGACGCTGCGCACGCGCGACCTCGGCGGCAACGCCGACAC
GCAGGCCTGCGGCCGCGCGGTCGAACAGGCGCTGGGCTGA

Upstream 100 bases:

>100_bases
CTCGCCCGGCAGCGGAGACCCCGCTTGTCTCTTCCCGCCATTGACGCCGCGAGACGCTGGCGCAAGTGAGACGCCGGGGG
CGGACACGAAGGCGACCACC

Downstream 100 bases:

>100_bases
GGGGCCGGCCGCGATGAGACCCGTCGTCGTCACCGTCGCAATCACCGGCTCGGTCGCCTGCAAGGCCGACAACCCGACCG
TGCCGATCACGCCCGCCGAA

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MGGTKRSYRIAVIPGDGIGKEVMPEGVRVLERAAARHGFEIVQDWFDFASCDYYEAHGRMMPEDWKAQIGSHDAIFFGAV
GMPERVPDHISLWGSLIQFRREFDQYVNLRPVRLMPGVPSPLAGRKPGDIDFWVVRENTEGEYSNAGGRMFAGTEREFAV
QESVFTRHGVDRVLKFAFELAQSRPKRHLTSATKSNGISITMPFWDERVRDVAEGYPEIRWDQYHIDILTAHFVLNPDRF
DVVVASNLFGDILSDLGPACTGTIGIAPSGNINPEQLYPSVFEPVHGSAPDIAGQGIANPIGQIWSGAMMLEHLGEREAA
AEIVAGIERVLTERTLRTRDLGGNADTQACGRAVEQALG

Sequences:

>Translated_359_residues
MGGTKRSYRIAVIPGDGIGKEVMPEGVRVLERAAARHGFEIVQDWFDFASCDYYEAHGRMMPEDWKAQIGSHDAIFFGAV
GMPERVPDHISLWGSLIQFRREFDQYVNLRPVRLMPGVPSPLAGRKPGDIDFWVVRENTEGEYSNAGGRMFAGTEREFAV
QESVFTRHGVDRVLKFAFELAQSRPKRHLTSATKSNGISITMPFWDERVRDVAEGYPEIRWDQYHIDILTAHFVLNPDRF
DVVVASNLFGDILSDLGPACTGTIGIAPSGNINPEQLYPSVFEPVHGSAPDIAGQGIANPIGQIWSGAMMLEHLGEREAA
AEIVAGIERVLTERTLRTRDLGGNADTQACGRAVEQALG
>Mature_358_residues
GGTKRSYRIAVIPGDGIGKEVMPEGVRVLERAAARHGFEIVQDWFDFASCDYYEAHGRMMPEDWKAQIGSHDAIFFGAVG
MPERVPDHISLWGSLIQFRREFDQYVNLRPVRLMPGVPSPLAGRKPGDIDFWVVRENTEGEYSNAGGRMFAGTEREFAVQ
ESVFTRHGVDRVLKFAFELAQSRPKRHLTSATKSNGISITMPFWDERVRDVAEGYPEIRWDQYHIDILTAHFVLNPDRFD
VVVASNLFGDILSDLGPACTGTIGIAPSGNINPEQLYPSVFEPVHGSAPDIAGQGIANPIGQIWSGAMMLEHLGEREAAA
EIVAGIERVLTERTLRTRDLGGNADTQACGRAVEQALG

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=337, Percent_Identity=32.9376854599407, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI4758582, Length=343, Percent_Identity=28.2798833819242, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI28178821, Length=348, Percent_Identity=28.448275862069, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI28178816, Length=359, Percent_Identity=28.4122562674095, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI28178838, Length=318, Percent_Identity=28.9308176100629, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178819, Length=186, Percent_Identity=31.7204301075269, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1788101, Length=354, Percent_Identity=66.9491525423729, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI87081683, Length=349, Percent_Identity=36.9627507163324, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1787381, Length=369, Percent_Identity=28.7262872628726, Blast_Score=114, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71986051, Length=343, Percent_Identity=32.3615160349854, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17550882, Length=344, Percent_Identity=27.3255813953488, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=350, Percent_Identity=27.4285714285714, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=342, Percent_Identity=27.7777777777778, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=37.0473537604457, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324291, Length=350, Percent_Identity=30.8571428571429, Blast_Score=135, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6324709, Length=358, Percent_Identity=29.608938547486, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319830, Length=367, Percent_Identity=29.9727520435967, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24643268, Length=350, Percent_Identity=32.2857142857143, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24643270, Length=350, Percent_Identity=32.2857142857143, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24661184, Length=342, Percent_Identity=30.9941520467836, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI281362242, Length=350, Percent_Identity=27.7142857142857, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648872, Length=350, Percent_Identity=27.7142857142857, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078635, Length=352, Percent_Identity=26.9886363636364, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078637, Length=352, Percent_Identity=27.2727272727273, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078633, Length=352, Percent_Identity=26.9886363636364, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24650122, Length=352, Percent_Identity=26.9886363636364, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078639, Length=263, Percent_Identity=30.0380228136882, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI20130355, Length=356, Percent_Identity=25.561797752809, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 39697; Mature: 39566

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGTKRSYRIAVIPGDGIGKEVMPEGVRVLERAAARHGFEIVQDWFDFASCDYYEAHGRM
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCC
MPEDWKAQIGSHDAIFFGAVGMPERVPDHISLWGSLIQFRREFDQYVNLRPVRLMPGVPS
CCHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
PLAGRKPGDIDFWVVRENTEGEYSNAGGRMFAGTEREFAVQESVFTRHGVDRVLKFAFEL
CCCCCCCCCCEEEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AQSRPKRHLTSATKSNGISITMPFWDERVRDVAEGYPEIRWDQYHIDILTAHFVLNPDRF
HHCCCHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCE
DVVVASNLFGDILSDLGPACTGTIGIAPSGNINPEQLYPSVFEPVHGSAPDIAGQGIANP
EEEEHHHHHHHHHHHCCCHHCCCEECCCCCCCCHHHHCHHHHCCCCCCCCCCCCCCCCHH
IGQIWSGAMMLEHLGEREAAAEIVAGIERVLTERTLRTRDLGGNADTQACGRAVEQALG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GGTKRSYRIAVIPGDGIGKEVMPEGVRVLERAAARHGFEIVQDWFDFASCDYYEAHGRM
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCC
MPEDWKAQIGSHDAIFFGAVGMPERVPDHISLWGSLIQFRREFDQYVNLRPVRLMPGVPS
CCHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
PLAGRKPGDIDFWVVRENTEGEYSNAGGRMFAGTEREFAVQESVFTRHGVDRVLKFAFEL
CCCCCCCCCCEEEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AQSRPKRHLTSATKSNGISITMPFWDERVRDVAEGYPEIRWDQYHIDILTAHFVLNPDRF
HHCCCHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCE
DVVVASNLFGDILSDLGPACTGTIGIAPSGNINPEQLYPSVFEPVHGSAPDIAGQGIANP
EEEEHHHHHHHHHHHCCCHHCCCEECCCCCCCCHHHHCHHHHCCCCCCCCCCCCCCCCHH
IGQIWSGAMMLEHLGEREAAAEIVAGIERVLTERTLRTRDLGGNADTQACGRAVEQALG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]