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The map label for this gene is pcm [H]

Identifier: 218532488

GI number: 218532488

Start: 4879794

End: 4880492

Strand: Reverse

Name: pcm [H]

Synonym: Mchl_4602

Alternate gene names: 218532488

Gene position: 4880492-4879794 (Counterclockwise)

Preceding gene: 218532489

Following gene: 218532487

Centisome position: 84.47

GC content: 76.54

Gene sequence:

>699_bases
ATGCCGCCTGAGGCCGGAGAGCCCGACGAACGGGCTGATCGGCTGTCCGCGGGGCTGGCGGAGGCGACCGGCAACGCCGC
CTTCGTGCTGGCCCTGCGCGAGCGGGGCGTGCGCGACACCGCCGTTCTGCGGGCGATGGAACAGGTCCCGCGCGAGCGCT
TCGCGCCGCCGGCCCTGCGCCCCCATGCGCGGCGCGACATCGCCCTGCCGCTCGCCTGCGGCCAGACCATGACCGCGCCG
AGCGTCGTCGCGCAGATGCTCGGCGCCCTCGACCTCGCCCCCGGCCAGCGCGTGCTGGAGGTCGGCACCGGCACGGGCTA
CGTCACCGCTCTGCTGGTCCGGCTCGGGGCCGCCCATGTGCGCAGCCTGGAGCGCTACGAGGGGCTCGCCCGCGCGGCGC
GGGCGCATCTGGGGCGCGACCTTTCCGACGTCACCGTCGAGACCAATGACGGGCTCGCGCCCGAAGTCGTTCGGGGCGGA
AGCTACGACCGCATCCTCGTCAACGGCAGCCTCGCCGCGCTGCCGCCGCACCTCCCCGCCGCGCTCAAATCTGGCGGCCG
GCTGGTGGTGGGCCATTGGACCGGGCGGGGGAGCCGGCTCGTGACGCTGACCCGCGACAGCGCGGCCGCCTACGCCCGGA
CGGAGGGGGCGGCGCTGCGCCTCGGCCCTCTTACGCCGGGCCGTGCGCTGGCGCCGTAG

Upstream 100 bases:

>100_bases
CTCGGTGACGCCGCTGCGGCTCGACCTCACCGACGAGCCGGAACTGACCCGCTTCGCCGCGGCGTTCCGGGCGTGAGCGG
GGGCGGCCGTCCGGGCCCTC

Downstream 100 bases:

>100_bases
CGGCTCGACGACGGTTCGGCGCGTGCGCATCGAGGGGCGGTCTCGCCCGCTCGAACACGCGACCGGCCGCGTTGCCAGAC
TCCCTCAACCTTTACGGAAC

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MPPEAGEPDERADRLSAGLAEATGNAAFVLALRERGVRDTAVLRAMEQVPRERFAPPALRPHARRDIALPLACGQTMTAP
SVVAQMLGALDLAPGQRVLEVGTGTGYVTALLVRLGAAHVRSLERYEGLARAARAHLGRDLSDVTVETNDGLAPEVVRGG
SYDRILVNGSLAALPPHLPAALKSGGRLVVGHWTGRGSRLVTLTRDSAAAYARTEGAALRLGPLTPGRALAP

Sequences:

>Translated_232_residues
MPPEAGEPDERADRLSAGLAEATGNAAFVLALRERGVRDTAVLRAMEQVPRERFAPPALRPHARRDIALPLACGQTMTAP
SVVAQMLGALDLAPGQRVLEVGTGTGYVTALLVRLGAAHVRSLERYEGLARAARAHLGRDLSDVTVETNDGLAPEVVRGG
SYDRILVNGSLAALPPHLPAALKSGGRLVVGHWTGRGSRLVTLTRDSAAAYARTEGAALRLGPLTPGRALAP
>Mature_231_residues
PPEAGEPDERADRLSAGLAEATGNAAFVLALRERGVRDTAVLRAMEQVPRERFAPPALRPHARRDIALPLACGQTMTAPS
VVAQMLGALDLAPGQRVLEVGTGTGYVTALLVRLGAAHVRSLERYEGLARAARAHLGRDLSDVTVETNDGLAPEVVRGGS
YDRILVNGSLAALPPHLPAALKSGGRLVVGHWTGRGSRLVTLTRDSAAAYARTEGAALRLGPLTPGRALAP

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=202, Percent_Identity=37.1287128712871, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24274; Mature: 24143

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPEAGEPDERADRLSAGLAEATGNAAFVLALRERGVRDTAVLRAMEQVPRERFAPPALR
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHCCCCCCC
PHARRDIALPLACGQTMTAPSVVAQMLGALDLAPGQRVLEVGTGTGYVTALLVRLGAAHV
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
RSLERYEGLARAARAHLGRDLSDVTVETNDGLAPEVVRGGSYDRILVNGSLAALPPHLPA
HHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCHHHHCCCCCCEEEECCCHHCCCCCCHH
ALKSGGRLVVGHWTGRGSRLVTLTRDSAAAYARTEGAALRLGPLTPGRALAP
HHHCCCEEEEEEECCCCCEEEEEECCCCHHHHHCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
PPEAGEPDERADRLSAGLAEATGNAAFVLALRERGVRDTAVLRAMEQVPRERFAPPALR
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHCCCCCCC
PHARRDIALPLACGQTMTAPSVVAQMLGALDLAPGQRVLEVGTGTGYVTALLVRLGAAHV
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
RSLERYEGLARAARAHLGRDLSDVTVETNDGLAPEVVRGGSYDRILVNGSLAALPPHLPA
HHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCHHHHCCCCCCEEEECCCHHCCCCCCHH
ALKSGGRLVVGHWTGRGSRLVTLTRDSAAAYARTEGAALRLGPLTPGRALAP
HHHCCCEEEEEEECCCCCEEEEEECCCCHHHHHCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA