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The map label for this gene is glcA [H]

Identifier: 218532479

GI number: 218532479

Start: 4869031

End: 4870770

Strand: Reverse

Name: glcA [H]

Synonym: Mchl_4593

Alternate gene names: 218532479

Gene position: 4870770-4869031 (Counterclockwise)

Preceding gene: 218532481

Following gene: 218532477

Centisome position: 84.3

GC content: 67.07

Gene sequence:

>1740_bases
GTGACACCCTGGAACCAGGTCTACGATCCATTCGGGAGCCAATGGCTCTCGACCTTCATGGCGTCGCTGCCGGTGATCGC
CCTGCTCGGCATGATCGCGAGCGGCAAGATCAAGGTGCACATCGCCGCCATCCTCGCCCTCGTCGTCGCCTTCCTCGTGG
CGGTGGTGGCCTTCGGTATGCCAACCGACCTCGCCGTCCGGGCGACCCTCCTCGGCGTCGTCACGGGCATGTTCCCGATC
GGCTGGATCATCCTCAACGTCATCTTCCTCTACCGGCTGACGGTGGAGAAGGGCTGGTTCGCGATCCTCCAGCAATCGGT
GGCCGGCATCACCGAGGACCGGCGCATCCAGCTCCTGTTGGTCGCCTTCGCCTTCGGCGCCTTCTTCGAGGGCGCGGGCG
GCTTCGGCACCCCGGTGGCCGTCACCGGCGCCATCCTGATCGGGCTCGGCTTCTCGCCGCTGGCCGCCTCCGGCCTCTCG
CTCATCGCCAACACCGCCCCCGTCGCCTACGGCGCACTCGGCGCGCCGGTGCAGGGTCTGGCCTCGGTGACGGGCTACGA
CCCCTACATCCTCGGCGCGATGATCGGCCGTCAGCTGCCGTTCTTCTCCGTGATCGTGCCGTTCTGGCTGATTTGGGTGT
TCGCGGGCTTCCGCGGCATGATCGCGATCTGGCCGCCGATCGCCGTCTGCGGCATCTCGTTTGCCGCGGCGCAGTTCCTG
ATCTCGAACTACATCAACCCCTGGATCGTCGATATCGGCGCCTCGCTGGTCTCGATGGCCGCCCTCGTCGCCTTCCTGAA
GGTGTGGCGTCCGGCCCAGCTGTGGACCTCGCCCGCCCTGCGCGGCCACGACCCGTCGGTCGGCTACGGTGAGCCGCGTC
CGGCCTCCCTCGGCGCGGGCGTCCCCGGTGATCTGCCCAAGGTGCATGTCGGCGGCCGCACTGCCTCGTCGAGCGAGATC
TTCATGGCCTGGGTGCCGTGGATCATCCTCTCGATCATCGTGGCGATCTGGGGCACCGGCTGGTTCAAGGGCATCGTCAA
CCCGATCTTCTCGTGGAAGTACGAGGTGCCGGGCCTGCACAACATGATCATGAAGGTGCCCCCGGTGGAGGCCGCGCCGA
AGGCCGAAGCCGCGATCTTCAACTTCACCTACATGTCCTACACCGGCACGGGCGTGCTGTTCGCCGCGATCATCTCCGGT
ATCATCATGCGGTTCTCGCCGGTGCGCCTCGTCACGGCGTATTTCGAGACGATCTGGGTGCTGCGCTACTCGCTCATCAC
CATCGCCGCGATGCTCGCCCTCGGCGTGCTCACCCGCTACGCCGGCGTGGATGCCACGCTCGGCCTCGCCTTCGCCGGCA
CGGGCATCCTCTACCCGTTCTTCGGCACGCTGCTGGGCTGGCTCGGCGTCGCGCTCACGGGTTCGGACACGGCTTCGAAC
GTGCTGTTCGGCGGCCTGCAGCGGATCACCTCCGAGCAGCTCGGATTGTCGGGCATCCTCATGGCCTCGGCGAACTCGTC
GGGCGGCGTGATGGGCAAGATGATCGACGCCCAGTCGATCGTCGTGGCCTCGACGGCGACGGGCTATTTCGGCCAGGAGA
GCAAGATCCTGCGCTTCGTGTTCTGGCACTCGATCGTGCTGGCCTGCCTCGTCGGCGGTCTCGTGATGCTCCAGGCCTAC
GTCTATCCGTTCACCGAGATGGTGATCCACCCGGCCGCGACGGCACCCGCCGCGCACTGA

Upstream 100 bases:

>100_bases
GAATACATGATGCCTTGCGTTGAGCGGGCCCAGCGGCTCTGCTAGCACCCTGTGCCAGAAGGTCCCGGCGGAAGATCGGG
GCCTGGGAGGAGCAAGGCCC

Downstream 100 bases:

>100_bases
CCGGCCGGATCGACGGCAGGCAAAACGAAGCCCGCCCCGAGCGATCGGGGCGGGCTTTTTCGTGGACCGGAATTAGCGTG
GGCCGGGCGCGTCCGGCTTT

Product: L-lactate transport

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MTPWNQVYDPFGSQWLSTFMASLPVIALLGMIASGKIKVHIAAILALVVAFLVAVVAFGMPTDLAVRATLLGVVTGMFPI
GWIILNVIFLYRLTVEKGWFAILQQSVAGITEDRRIQLLLVAFAFGAFFEGAGGFGTPVAVTGAILIGLGFSPLAASGLS
LIANTAPVAYGALGAPVQGLASVTGYDPYILGAMIGRQLPFFSVIVPFWLIWVFAGFRGMIAIWPPIAVCGISFAAAQFL
ISNYINPWIVDIGASLVSMAALVAFLKVWRPAQLWTSPALRGHDPSVGYGEPRPASLGAGVPGDLPKVHVGGRTASSSEI
FMAWVPWIILSIIVAIWGTGWFKGIVNPIFSWKYEVPGLHNMIMKVPPVEAAPKAEAAIFNFTYMSYTGTGVLFAAIISG
IIMRFSPVRLVTAYFETIWVLRYSLITIAAMLALGVLTRYAGVDATLGLAFAGTGILYPFFGTLLGWLGVALTGSDTASN
VLFGGLQRITSEQLGLSGILMASANSSGGVMGKMIDAQSIVVASTATGYFGQESKILRFVFWHSIVLACLVGGLVMLQAY
VYPFTEMVIHPAATAPAAH

Sequences:

>Translated_579_residues
MTPWNQVYDPFGSQWLSTFMASLPVIALLGMIASGKIKVHIAAILALVVAFLVAVVAFGMPTDLAVRATLLGVVTGMFPI
GWIILNVIFLYRLTVEKGWFAILQQSVAGITEDRRIQLLLVAFAFGAFFEGAGGFGTPVAVTGAILIGLGFSPLAASGLS
LIANTAPVAYGALGAPVQGLASVTGYDPYILGAMIGRQLPFFSVIVPFWLIWVFAGFRGMIAIWPPIAVCGISFAAAQFL
ISNYINPWIVDIGASLVSMAALVAFLKVWRPAQLWTSPALRGHDPSVGYGEPRPASLGAGVPGDLPKVHVGGRTASSSEI
FMAWVPWIILSIIVAIWGTGWFKGIVNPIFSWKYEVPGLHNMIMKVPPVEAAPKAEAAIFNFTYMSYTGTGVLFAAIISG
IIMRFSPVRLVTAYFETIWVLRYSLITIAAMLALGVLTRYAGVDATLGLAFAGTGILYPFFGTLLGWLGVALTGSDTASN
VLFGGLQRITSEQLGLSGILMASANSSGGVMGKMIDAQSIVVASTATGYFGQESKILRFVFWHSIVLACLVGGLVMLQAY
VYPFTEMVIHPAATAPAAH
>Mature_578_residues
TPWNQVYDPFGSQWLSTFMASLPVIALLGMIASGKIKVHIAAILALVVAFLVAVVAFGMPTDLAVRATLLGVVTGMFPIG
WIILNVIFLYRLTVEKGWFAILQQSVAGITEDRRIQLLLVAFAFGAFFEGAGGFGTPVAVTGAILIGLGFSPLAASGLSL
IANTAPVAYGALGAPVQGLASVTGYDPYILGAMIGRQLPFFSVIVPFWLIWVFAGFRGMIAIWPPIAVCGISFAAAQFLI
SNYINPWIVDIGASLVSMAALVAFLKVWRPAQLWTSPALRGHDPSVGYGEPRPASLGAGVPGDLPKVHVGGRTASSSEIF
MAWVPWIILSIIVAIWGTGWFKGIVNPIFSWKYEVPGLHNMIMKVPPVEAAPKAEAAIFNFTYMSYTGTGVLFAAIISGI
IMRFSPVRLVTAYFETIWVLRYSLITIAAMLALGVLTRYAGVDATLGLAFAGTGILYPFFGTLLGWLGVALTGSDTASNV
LFGGLQRITSEQLGLSGILMASANSSGGVMGKMIDAQSIVVASTATGYFGQESKILRFVFWHSIVLACLVGGLVMLQAYV
YPFTEMVIHPAATAPAAH

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=575, Percent_Identity=44.3478260869565, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1790031, Length=566, Percent_Identity=41.1660777385159, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 61447; Mature: 61316

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TPWNQVYDPFGSQWLSTFMASLPVIALLGMIASGKIKVHIAAILALVVAFLVAVVAFGM
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCC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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]