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The map label for this gene is 218532463

Identifier: 218532463

GI number: 218532463

Start: 4847335

End: 4848699

Strand: Reverse

Name: 218532463

Synonym: Mchl_4575

Alternate gene names: NA

Gene position: 4848699-4847335 (Counterclockwise)

Preceding gene: 218532464

Following gene: 218532462

Centisome position: 83.92

GC content: 68.28

Gene sequence:

>1365_bases
ATGCTGTATCCTCTTTACGAGGCCGGCCATCTTATGCTCGCACCGATGCGCTTGGCGGCGGAAGCCACCAAACTTGCCTG
CGAAAACCCGTTCAATCCCTTCGCCTACGCCCCGCAGAGCCGCACCATGGCTGCGGGCTGCGAGATGTTCGAGCGCGCCA
CCCGCGTCTACGCCAAGCCGGCCTTCGGGCTCGACGTGCCGGAGCGGGTGGTCTGGGAGCGCCCCTTCTGCCGCGTCGTC
GCCTTCGGCGAGCCCTCCGCGGAACTCGAGGCGAAGCCGAAGCTGCTGATCGTCGCGCCGATGTCAGGTCACTACGCCAC
GCTGCTGCGCGGCACGGTCGAGGCGTTTCTCCCTAGCCATCAGGTCTTCATCACCGATTGGTCCGATGCGCGTCAGGTGC
CGGCGAGCGCCGGCCGGTTCGGCCTCGACGATTACATCGATACCTGCATCGCCCTGTTCGCGGCGCTGGGACCGGATCTC
CACGTCGCAGCGGTCTGTCAGCCCTCGGTGCCGGTGCTCGCCGCCATCGCCCGCATGGAGGCGGAGGATCACCCGCTCGT
GCCGCGCTCGGCCGTGTTGATGGGCGGTCCCGTCGATACCCGCCGCTCGCCGACTGCCGTCAACCGCATGGCCGAGGAGA
AGGGCTTCGCGTGGTTCGAGCGGCACTGCATCCACAGGGTGCCGGGCGGATATCCGGGAGCCGGCCGCGCGGTCTATCCA
GGCTTCCTTCAGCTCGCTGGCTTCATGGGGATGAACCTTGAGCGCCACCGCGACGCCCACCACGCGATGTTCGACCATCT
CGTGCGCGGCGACGGCGACTCGGCCGCCCGCCATCGCGCCTTCTACGACGAGTACCTCGCAGTCATGGACCTGACCGCTG
AGTTCTACCTTGAGACGATCCAGCGGGTCTTCATCAGTCACGACCTGCCCCGCGGCACCCTGCGTCATCGCGGCGAACGC
GTCGATCTCGGCGCCATCCGCCGCTGCCACTTGATGGCGGTGGAGGGCGAGAAGGACGACATCACCGGCCTCGGCCAGAC
CAAGGCTGCGCTCGATCTCGCGGTGAACCTGCCCGAGGCGGCCAAGACCTACCACATGCAGCCGGGCGCCGGGCATTACG
GCATCTTCAACGGCTCGCGCTTCCGCCAGGATATCGTGCCGTTGGTCTGCAGCTTCATGGAACGCAGCCTGCGGCCGGCT
GCGCCGCGCCCAGCCCCCGTGGTGCCGGCACCGGAGCCGCACCCAATTATCCTGCGCCACGGCCCCATCATGCAGCCTTC
GCGCGCCACGCCCACCCGCGCCATCGTGTGGCCCGAGCCTCTGGTCGATCGACCGGGAGCGATCCCGCAGCGGATCGCCC
TGTAA

Upstream 100 bases:

>100_bases
GAGCAAACGAAACTTTCCTTGGGGTTCGGAGTTTTAACCCTCACGAACTCAAGAAAAGGACAGCTTGATTCAAGCGAGCT
GCTCCCAGGGAAGCGCTCGC

Downstream 100 bases:

>100_bases
CCGCCGCTCCCGACCGTTCTCCACCATCCGCCCATCGAAGCGGCCGCCGATCCCCTCGGCGGCCGCTTTGCCGTGGAAGG
GACGCTGCGACCCCACCGCG

Product: polyhydroxyalkanoate depolymerase, intracellular

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Poly(3-Hydroxyalkanoate)Synthetase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MLYPLYEAGHLMLAPMRLAAEATKLACENPFNPFAYAPQSRTMAAGCEMFERATRVYAKPAFGLDVPERVVWERPFCRVV
AFGEPSAELEAKPKLLIVAPMSGHYATLLRGTVEAFLPSHQVFITDWSDARQVPASAGRFGLDDYIDTCIALFAALGPDL
HVAAVCQPSVPVLAAIARMEAEDHPLVPRSAVLMGGPVDTRRSPTAVNRMAEEKGFAWFERHCIHRVPGGYPGAGRAVYP
GFLQLAGFMGMNLERHRDAHHAMFDHLVRGDGDSAARHRAFYDEYLAVMDLTAEFYLETIQRVFISHDLPRGTLRHRGER
VDLGAIRRCHLMAVEGEKDDITGLGQTKAALDLAVNLPEAAKTYHMQPGAGHYGIFNGSRFRQDIVPLVCSFMERSLRPA
APRPAPVVPAPEPHPIILRHGPIMQPSRATPTRAIVWPEPLVDRPGAIPQRIAL

Sequences:

>Translated_454_residues
MLYPLYEAGHLMLAPMRLAAEATKLACENPFNPFAYAPQSRTMAAGCEMFERATRVYAKPAFGLDVPERVVWERPFCRVV
AFGEPSAELEAKPKLLIVAPMSGHYATLLRGTVEAFLPSHQVFITDWSDARQVPASAGRFGLDDYIDTCIALFAALGPDL
HVAAVCQPSVPVLAAIARMEAEDHPLVPRSAVLMGGPVDTRRSPTAVNRMAEEKGFAWFERHCIHRVPGGYPGAGRAVYP
GFLQLAGFMGMNLERHRDAHHAMFDHLVRGDGDSAARHRAFYDEYLAVMDLTAEFYLETIQRVFISHDLPRGTLRHRGER
VDLGAIRRCHLMAVEGEKDDITGLGQTKAALDLAVNLPEAAKTYHMQPGAGHYGIFNGSRFRQDIVPLVCSFMERSLRPA
APRPAPVVPAPEPHPIILRHGPIMQPSRATPTRAIVWPEPLVDRPGAIPQRIAL
>Mature_454_residues
MLYPLYEAGHLMLAPMRLAAEATKLACENPFNPFAYAPQSRTMAAGCEMFERATRVYAKPAFGLDVPERVVWERPFCRVV
AFGEPSAELEAKPKLLIVAPMSGHYATLLRGTVEAFLPSHQVFITDWSDARQVPASAGRFGLDDYIDTCIALFAALGPDL
HVAAVCQPSVPVLAAIARMEAEDHPLVPRSAVLMGGPVDTRRSPTAVNRMAEEKGFAWFERHCIHRVPGGYPGAGRAVYP
GFLQLAGFMGMNLERHRDAHHAMFDHLVRGDGDSAARHRAFYDEYLAVMDLTAEFYLETIQRVFISHDLPRGTLRHRGER
VDLGAIRRCHLMAVEGEKDDITGLGQTKAALDLAVNLPEAAKTYHMQPGAGHYGIFNGSRFRQDIVPLVCSFMERSLRPA
APRPAPVVPAPEPHPIILRHGPIMQPSRATPTRAIVWPEPLVDRPGAIPQRIAL

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 50079; Mature: 50079

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPLYEAGHLMLAPMRLAAEATKLACENPFNPFAYAPQSRTMAAGCEMFERATRVYAKP
CCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
AFGLDVPERVVWERPFCRVVAFGEPSAELEAKPKLLIVAPMSGHYATLLRGTVEAFLPSH
CCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCC
QVFITDWSDARQVPASAGRFGLDDYIDTCIALFAALGPDLHVAAVCQPSVPVLAAIARME
EEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHC
AEDHPLVPRSAVLMGGPVDTRRSPTAVNRMAEEKGFAWFERHCIHRVPGGYPGAGRAVYP
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCH
GFLQLAGFMGMNLERHRDAHHAMFDHLVRGDGDSAARHRAFYDEYLAVMDLTAEFYLETI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRVFISHDLPRGTLRHRGERVDLGAIRRCHLMAVEGEKDDITGLGQTKAALDLAVNLPEA
HHHHHHCCCCCCHHHHCCCCCCHHHHCEEEEEEECCCCCCCCCCCCHHHHHEEEECCCHH
AKTYHMQPGAGHYGIFNGSRFRQDIVPLVCSFMERSLRPAAPRPAPVVPAPEPHPIILRH
HHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEC
GPIMQPSRATPTRAIVWPEPLVDRPGAIPQRIAL
CCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MLYPLYEAGHLMLAPMRLAAEATKLACENPFNPFAYAPQSRTMAAGCEMFERATRVYAKP
CCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
AFGLDVPERVVWERPFCRVVAFGEPSAELEAKPKLLIVAPMSGHYATLLRGTVEAFLPSH
CCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCC
QVFITDWSDARQVPASAGRFGLDDYIDTCIALFAALGPDLHVAAVCQPSVPVLAAIARME
EEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHC
AEDHPLVPRSAVLMGGPVDTRRSPTAVNRMAEEKGFAWFERHCIHRVPGGYPGAGRAVYP
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCH
GFLQLAGFMGMNLERHRDAHHAMFDHLVRGDGDSAARHRAFYDEYLAVMDLTAEFYLETI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRVFISHDLPRGTLRHRGERVDLGAIRRCHLMAVEGEKDDITGLGQTKAALDLAVNLPEA
HHHHHHCCCCCCHHHHCCCCCCHHHHCEEEEEEECCCCCCCCCCCCHHHHHEEEECCCHH
AKTYHMQPGAGHYGIFNGSRFRQDIVPLVCSFMERSLRPAAPRPAPVVPAPEPHPIILRH
HHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEC
GPIMQPSRATPTRAIVWPEPLVDRPGAIPQRIAL
CCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA