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The map label for this gene is potH [H]

Identifier: 218532452

GI number: 218532452

Start: 4831573

End: 4832577

Strand: Reverse

Name: potH [H]

Synonym: Mchl_4564

Alternate gene names: 218532452

Gene position: 4832577-4831573 (Counterclockwise)

Preceding gene: 218532453

Following gene: 218532451

Centisome position: 83.64

GC content: 69.75

Gene sequence:

>1005_bases
ATGAGCGGGCTGTCGCGGCAGCCACGACCGACAGGCGATGACGGCTCGTCGCCGGTTGCCCCCGCCCATGCGGGGCGGCT
GTCTCAGCGCCTGGGGCGCGGTCTCGTCCGCGGCCTGCCGCTGCTGTGGCTGGCGCTGTTCTTCCTCGTGCCCTTCGCGG
TGACGCTGAAGATCAGCTTCTCCTCGCCGGCCACCGCGCAGCCGCCCTACCTGCCCCTGCTCGAATGGGATGGCGGCCTG
GAGGGCTGGCGCGAGTTCTTCGAGGCGCTCGACTTCCAGAACTACCAGATGATCGCGGCCGACGCGCTCTACCGCGACGC
CGCCCTCTCCTCGCTGGGCTATGCCGCGCTCGCCACCGCGATCCTCGTCGGGATCGGCACGCCGACGGCCTACGCCATGG
CCCGCGCCTCGGCGCGCTGGCAGCCGATCCTCGTGGCGCTGGTCATCGTGCCGTTCTGGACGAGTTTTTTGATCCGGGTC
TATGCCTGGATCGCGATCCTCAAGCCCGAGGGGCTGCTCAACGCGGCCCTGCTGAAACTCGGCCTGATCGCCGCGCCACT
GCCGATCCTCGATAGTCCCTATGGCGTGCTGATCGGGCTCACCTACGCCTACCTGCCCTTCATGGTGCTGCCGCTCTACG
CGGTGATGAGCCGGCTCGATCCGGCGCTCACCGAGGCCGCCGCCGATCTCGGCGCGTCGCGGGCGCGGGCCTTCTTCACC
GTCACCCTGCCGCTCAGCCTGCCCGGCCTCGCCGCCGGGGCGTTGCTCTGCTTCATCCCGATGGTCGGCGAGTTCATCAT
CCCCGATCTGCTCGGCGGCTCCGACACGCTGATGCTCGGGCGCGTGCTGTGGAGCGAGTTCTTTTCCAACCGCGACTGGC
CGCTGGCCTCGGCGGTGGCGGTGCTGTTGCTGATCCTCGTGGTCGGGCCCGTGGTGCTGTTCCGCGAGGCCGAGCTGCGC
CGCGAGGAAGCAGTGGAAGGGGCTCAGGAAGGGGCTCAGCGATGA

Upstream 100 bases:

>100_bases
TACGCGCCTCCGCCGCCCTGCCGGCGCCCGGCCCGATCCCGGCCGTCGGCGAGACCGTCACCCTCGGGGTGCCCGCGGAA
GCCGCCTGGATCCTGCCGGC

Downstream 100 bases:

>100_bases
GCCTCTTCGCCCGCACGGCGCTGACCCTCGCGCTGGCCTTCCTCTACGCGCCGATCCTCGTGCTGGTCGCCTATTCCTTC
AACGATTCGAAGCTCGTGAC

Product: binding-protein-dependent transporters inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSGLSRQPRPTGDDGSSPVAPAHAGRLSQRLGRGLVRGLPLLWLALFFLVPFAVTLKISFSSPATAQPPYLPLLEWDGGL
EGWREFFEALDFQNYQMIAADALYRDAALSSLGYAALATAILVGIGTPTAYAMARASARWQPILVALVIVPFWTSFLIRV
YAWIAILKPEGLLNAALLKLGLIAAPLPILDSPYGVLIGLTYAYLPFMVLPLYAVMSRLDPALTEAAADLGASRARAFFT
VTLPLSLPGLAAGALLCFIPMVGEFIIPDLLGGSDTLMLGRVLWSEFFSNRDWPLASAVAVLLLILVVGPVVLFREAELR
REEAVEGAQEGAQR

Sequences:

>Translated_334_residues
MSGLSRQPRPTGDDGSSPVAPAHAGRLSQRLGRGLVRGLPLLWLALFFLVPFAVTLKISFSSPATAQPPYLPLLEWDGGL
EGWREFFEALDFQNYQMIAADALYRDAALSSLGYAALATAILVGIGTPTAYAMARASARWQPILVALVIVPFWTSFLIRV
YAWIAILKPEGLLNAALLKLGLIAAPLPILDSPYGVLIGLTYAYLPFMVLPLYAVMSRLDPALTEAAADLGASRARAFFT
VTLPLSLPGLAAGALLCFIPMVGEFIIPDLLGGSDTLMLGRVLWSEFFSNRDWPLASAVAVLLLILVVGPVVLFREAELR
REEAVEGAQEGAQR
>Mature_333_residues
SGLSRQPRPTGDDGSSPVAPAHAGRLSQRLGRGLVRGLPLLWLALFFLVPFAVTLKISFSSPATAQPPYLPLLEWDGGLE
GWREFFEALDFQNYQMIAADALYRDAALSSLGYAALATAILVGIGTPTAYAMARASARWQPILVALVIVPFWTSFLIRVY
AWIAILKPEGLLNAALLKLGLIAAPLPILDSPYGVLIGLTYAYLPFMVLPLYAVMSRLDPALTEAAADLGASRARAFFTV
TLPLSLPGLAAGALLCFIPMVGEFIIPDLLGGSDTLMLGRVLWSEFFSNRDWPLASAVAVLLLILVVGPVVLFREAELRR
EEAVEGAQEGAQR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=296, Percent_Identity=49.6621621621622, Blast_Score=260, Evalue=9e-71,
Organism=Escherichia coli, GI1787369, Length=234, Percent_Identity=40.1709401709402, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787713, Length=247, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35965; Mature: 35834

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLSRQPRPTGDDGSSPVAPAHAGRLSQRLGRGLVRGLPLLWLALFFLVPFAVTLKISF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
SSPATAQPPYLPLLEWDGGLEGWREFFEALDFQNYQMIAADALYRDAALSSLGYAALATA
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILVGIGTPTAYAMARASARWQPILVALVIVPFWTSFLIRVYAWIAILKPEGLLNAALLKL
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GLIAAPLPILDSPYGVLIGLTYAYLPFMVLPLYAVMSRLDPALTEAAADLGASRARAFFT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCHHHCCEEEE
VTLPLSLPGLAAGALLCFIPMVGEFIIPDLLGGSDTLMLGRVLWSEFFSNRDWPLASAVA
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
VLLLILVVGPVVLFREAELRREEAVEGAQEGAQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGLSRQPRPTGDDGSSPVAPAHAGRLSQRLGRGLVRGLPLLWLALFFLVPFAVTLKISF
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
SSPATAQPPYLPLLEWDGGLEGWREFFEALDFQNYQMIAADALYRDAALSSLGYAALATA
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILVGIGTPTAYAMARASARWQPILVALVIVPFWTSFLIRVYAWIAILKPEGLLNAALLKL
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GLIAAPLPILDSPYGVLIGLTYAYLPFMVLPLYAVMSRLDPALTEAAADLGASRARAFFT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCHHHCCEEEE
VTLPLSLPGLAAGALLCFIPMVGEFIIPDLLGGSDTLMLGRVLWSEFFSNRDWPLASAVA
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
VLLLILVVGPVVLFREAELRREEAVEGAQEGAQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]