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The map label for this gene is alkK [H]

Identifier: 218532428

GI number: 218532428

Start: 4809562

End: 4811193

Strand: Reverse

Name: alkK [H]

Synonym: Mchl_4540

Alternate gene names: 218532428

Gene position: 4811193-4809562 (Counterclockwise)

Preceding gene: 218532429

Following gene: 218532425

Centisome position: 83.27

GC content: 69.06

Gene sequence:

>1632_bases
ATGGCGCGCACGACGATCTACGACCGCGATCTCGACCCGAATCCCGCGAACTTCCAGCCGCTCACGCCGCTGACCTATCT
CGATCGCGCCGCCCGCACCTTCCCGGACCGGGTTGCGGTGATCCACGGGCCCTTGCGCCGCTCCTACGCTGACCTCTACG
CCCGCTGCCGCCGCCTTGCCGCGGCTTTGGCCGCCCGCGGCATCGGCCGCGGCGACACGGTGGCGGTGCTGCTCGCCAAC
ACGCCCGCGATGATCGAGTGTCACTACGGCGTGCCGATGACGGGGGCGGTGCTCAACACCCTCAACACCCGCCTCGACGC
GGCGGCTCTGGCCTTCTGCCTCGACCACGGCGAAGCCAAGGTCTTCATCGTCGATCGCGAGTTCGCGCGGGTCGGGCGCG
AGGCGCTCGACAAGGCCGGGGTCTCACCCCTCGTCATCGATTACGACGACCCTGAATTCACCGGAGACAGCGCGCCCGTC
GGCGAGACCGACTACGAAGATTTCCTCGCCGCCGGCGATCCCGATTTCGACTGGGCGATGCCGGGCGACGAGTGGGACGC
GATCTCGCTGAACTACACCTCCGGCACCACCGGCGATCCGAAAGGCGTGGTCTATCACCACCGCGGTGCGGCCCTACTCT
CGCTCGGCAACGTCATCACCGCCGGGCTGCCGCAGCACGCGGTCTATCTCTGGACCCTGCCGATGTTTCACTGCAACGGC
TGGTGCTTCCCCTGGACGCTCTCGATCGTCGCCGGCACCCATGTCTGCCTGCGGCAGGTGCGGGCGCCCGCGATGTACGC
GGCTCTGGCCGAGCACGGCGTCACCCACCTGTCGGGCGCGCCGATCGTGATGTCGACGCTGCTGAACGCCCCCGAGGCGC
AGAAGCGCCCCCTGCCCCGCCGGGTCCACTTCCTCACTGCCGCCGCGCCGCCGCCCGAGGCGGTGCTCGCCGCCATGGGC
GAGGCCGGCTTCGACGTCACCCATCTCTACGGCCTGACCGAGACCTACGGCCCAGCGGTCGTGAATGCGTGGCACGAGGA
TTGGGATGCGCTGTCCCGCGACGAGCAGGCCAGGAAGAAGGCCCGCCAGGGCGTGCGCTACCCCGTCCTAGAAGGGCTCG
ACGTGCGCGACCCCGAGACGATGGCATCCCTGCCCGCCGACGGCACCAGCCTCGGCGAGGTGATGTTCCGCGGCAATGTG
GTGATGCGCGGCTACCTGAAGAACCCGGCCTCGACCGAGGCCGCCTTCAAGGGCGGCTGGTTCCGCTCCGGCGATCTCGG
GGTGAAGCATCCCGACGGCTACATCCAACTGAAAGACCGCTCGAAGGACATCATCATCTCGGGCGGCGAGAACATCTCCT
CGATCGAGGTGGAGGACGCGCTGTTCAAGCACCCGGCGGTCGCGGCCGCCGCCGTCGTCGCCAAGCCCGACGCGAAATGG
GGCGAGACGCCCTGCGCCTTCGTCGAGCTGAAGGAGGGGCGGGAGGCAACCTCCGAGGAACTGGTCGCATGGTGCCGCGA
GCGGCTCGCCCCCTACAAACTGCCGCGCCACGTGGTGTTCGGCGAACTGCCGAAGACCTCGACGGGCAAGGTGCAGAAGT
TCGTGTTGCGGGAGAAGGCGCGGGAGGAGTGA

Upstream 100 bases:

>100_bases
ACCTGACAAGGCTCCCGGGCTCGCGCCCGCGGGGCCTTTTCTATGCCGTTGATCGCCCGCGGGGCTCGGATAATCTCCCC
CTGAAACGGGAGGCGAGACC

Downstream 100 bases:

>100_bases
AGTCCCAATACGCCATCCGGAAAGGATTGGCGGGACGAGGTATGGGCCGAGCGCTTCACTCCACGCCCAATTGCTGCGCG
GCGCTCATGATCGCGGTCAT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MARTTIYDRDLDPNPANFQPLTPLTYLDRAARTFPDRVAVIHGPLRRSYADLYARCRRLAAALAARGIGRGDTVAVLLAN
TPAMIECHYGVPMTGAVLNTLNTRLDAAALAFCLDHGEAKVFIVDREFARVGREALDKAGVSPLVIDYDDPEFTGDSAPV
GETDYEDFLAAGDPDFDWAMPGDEWDAISLNYTSGTTGDPKGVVYHHRGAALLSLGNVITAGLPQHAVYLWTLPMFHCNG
WCFPWTLSIVAGTHVCLRQVRAPAMYAALAEHGVTHLSGAPIVMSTLLNAPEAQKRPLPRRVHFLTAAAPPPEAVLAAMG
EAGFDVTHLYGLTETYGPAVVNAWHEDWDALSRDEQARKKARQGVRYPVLEGLDVRDPETMASLPADGTSLGEVMFRGNV
VMRGYLKNPASTEAAFKGGWFRSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVAKPDAKW
GETPCAFVELKEGREATSEELVAWCRERLAPYKLPRHVVFGELPKTSTGKVQKFVLREKAREE

Sequences:

>Translated_543_residues
MARTTIYDRDLDPNPANFQPLTPLTYLDRAARTFPDRVAVIHGPLRRSYADLYARCRRLAAALAARGIGRGDTVAVLLAN
TPAMIECHYGVPMTGAVLNTLNTRLDAAALAFCLDHGEAKVFIVDREFARVGREALDKAGVSPLVIDYDDPEFTGDSAPV
GETDYEDFLAAGDPDFDWAMPGDEWDAISLNYTSGTTGDPKGVVYHHRGAALLSLGNVITAGLPQHAVYLWTLPMFHCNG
WCFPWTLSIVAGTHVCLRQVRAPAMYAALAEHGVTHLSGAPIVMSTLLNAPEAQKRPLPRRVHFLTAAAPPPEAVLAAMG
EAGFDVTHLYGLTETYGPAVVNAWHEDWDALSRDEQARKKARQGVRYPVLEGLDVRDPETMASLPADGTSLGEVMFRGNV
VMRGYLKNPASTEAAFKGGWFRSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVAKPDAKW
GETPCAFVELKEGREATSEELVAWCRERLAPYKLPRHVVFGELPKTSTGKVQKFVLREKAREE
>Mature_542_residues
ARTTIYDRDLDPNPANFQPLTPLTYLDRAARTFPDRVAVIHGPLRRSYADLYARCRRLAAALAARGIGRGDTVAVLLANT
PAMIECHYGVPMTGAVLNTLNTRLDAAALAFCLDHGEAKVFIVDREFARVGREALDKAGVSPLVIDYDDPEFTGDSAPVG
ETDYEDFLAAGDPDFDWAMPGDEWDAISLNYTSGTTGDPKGVVYHHRGAALLSLGNVITAGLPQHAVYLWTLPMFHCNGW
CFPWTLSIVAGTHVCLRQVRAPAMYAALAEHGVTHLSGAPIVMSTLLNAPEAQKRPLPRRVHFLTAAAPPPEAVLAAMGE
AGFDVTHLYGLTETYGPAVVNAWHEDWDALSRDEQARKKARQGVRYPVLEGLDVRDPETMASLPADGTSLGEVMFRGNVV
MRGYLKNPASTEAAFKGGWFRSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVAKPDAKWG
ETPCAFVELKEGREATSEELVAWCRERLAPYKLPRHVVFGELPKTSTGKVQKFVLREKAREE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=554, Percent_Identity=27.7978339350181, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI187761345, Length=546, Percent_Identity=27.1062271062271, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI187761343, Length=546, Percent_Identity=27.1062271062271, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI157311624, Length=517, Percent_Identity=27.6595744680851, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI157311622, Length=517, Percent_Identity=27.6595744680851, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI58082049, Length=517, Percent_Identity=27.4661508704062, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI122937307, Length=522, Percent_Identity=25.8620689655172, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI42544132, Length=512, Percent_Identity=25.390625, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI38505220, Length=523, Percent_Identity=26.5774378585086, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI115511026, Length=522, Percent_Identity=26.0536398467433, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI13375727, Length=543, Percent_Identity=23.9410681399632, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI8923896, Length=150, Percent_Identity=34, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI28416953, Length=138, Percent_Identity=32.6086956521739, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI253970489, Length=150, Percent_Identity=34, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI145693145, Length=523, Percent_Identity=32.6959847036329, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1788107, Length=566, Percent_Identity=26.5017667844523, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI221142682, Length=503, Percent_Identity=24.6520874751491, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1786810, Length=532, Percent_Identity=28.0075187969925, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1790505, Length=131, Percent_Identity=34.3511450381679, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71994694, Length=560, Percent_Identity=26.6071428571429, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI71994690, Length=560, Percent_Identity=26.6071428571429, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI71994703, Length=560, Percent_Identity=26.6071428571429, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17560308, Length=539, Percent_Identity=28.2003710575139, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=366, Percent_Identity=31.1475409836066, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=515, Percent_Identity=24.8543689320388, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=537, Percent_Identity=23.463687150838, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=523, Percent_Identity=25.0478011472275, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=528, Percent_Identity=23.1060606060606, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=523, Percent_Identity=24.282982791587, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=524, Percent_Identity=22.9007633587786, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=515, Percent_Identity=23.495145631068, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=25.207756232687, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=495, Percent_Identity=25.6565656565657, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323182, Length=141, Percent_Identity=31.2056737588652, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319264, Length=131, Percent_Identity=33.587786259542, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581924, Length=575, Percent_Identity=26.9565217391304, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=24.6679316888046, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18859661, Length=551, Percent_Identity=27.0417422867514, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922652, Length=505, Percent_Identity=23.7623762376238, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130357, Length=551, Percent_Identity=22.8675136116152, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281365686, Length=476, Percent_Identity=21.8487394957983, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24653035, Length=512, Percent_Identity=22.4609375, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI62472339, Length=127, Percent_Identity=33.8582677165354, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24667959, Length=127, Percent_Identity=33.8582677165354, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24667955, Length=127, Percent_Identity=33.8582677165354, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921316, Length=221, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 59211; Mature: 59080

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTTIYDRDLDPNPANFQPLTPLTYLDRAARTFPDRVAVIHGPLRRSYADLYARCRRLA
CCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHH
AALAARGIGRGDTVAVLLANTPAMIECHYGVPMTGAVLNTLNTRLDAAALAFCLDHGEAK
HHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VFIVDREFARVGREALDKAGVSPLVIDYDDPEFTGDSAPVGETDYEDFLAAGDPDFDWAM
EEEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
PGDEWDAISLNYTSGTTGDPKGVVYHHRGAALLSLGNVITAGLPQHAVYLWTLPMFHCNG
CCCCCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCC
WCFPWTLSIVAGTHVCLRQVRAPAMYAALAEHGVTHLSGAPIVMSTLLNAPEAQKRPLPR
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHCCCCHHHHHHHHCCCCHHCCCCCC
RVHFLTAAAPPPEAVLAAMGEAGFDVTHLYGLTETYGPAVVNAWHEDWDALSRDEQARKK
EEEEEEECCCCHHHHHHHHCCCCCCHHHEECCHHHCCCHHHHHHHHHHHHHCCHHHHHHH
ARQGVRYPVLEGLDVRDPETMASLPADGTSLGEVMFRGNVVMRGYLKNPASTEAAFKGGW
HHCCCCCCHHCCCCCCCCHHHHHCCCCCCCHHHHHEECCEEEEEECCCCCCCCHHHCCCC
FRSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVAKPDAKW
EECCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCCHHHHHHEEECCCCCC
GETPCAFVELKEGREATSEELVAWCRERLAPYKLPRHVVFGELPKTSTGKVQKFVLREKA
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCCHHHHHHHHHHHH
REE
CCC
>Mature Secondary Structure 
ARTTIYDRDLDPNPANFQPLTPLTYLDRAARTFPDRVAVIHGPLRRSYADLYARCRRLA
CCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHH
AALAARGIGRGDTVAVLLANTPAMIECHYGVPMTGAVLNTLNTRLDAAALAFCLDHGEAK
HHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VFIVDREFARVGREALDKAGVSPLVIDYDDPEFTGDSAPVGETDYEDFLAAGDPDFDWAM
EEEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
PGDEWDAISLNYTSGTTGDPKGVVYHHRGAALLSLGNVITAGLPQHAVYLWTLPMFHCNG
CCCCCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCC
WCFPWTLSIVAGTHVCLRQVRAPAMYAALAEHGVTHLSGAPIVMSTLLNAPEAQKRPLPR
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHCCCCHHHHHHHHCCCCHHCCCCCC
RVHFLTAAAPPPEAVLAAMGEAGFDVTHLYGLTETYGPAVVNAWHEDWDALSRDEQARKK
EEEEEEECCCCHHHHHHHHCCCCCCHHHEECCHHHCCCHHHHHHHHHHHHHCCHHHHHHH
ARQGVRYPVLEGLDVRDPETMASLPADGTSLGEVMFRGNVVMRGYLKNPASTEAAFKGGW
HHCCCCCCHHCCCCCCCCHHHHHCCCCCCCHHHHHEECCEEEEEECCCCCCCCHHHCCCC
FRSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVAKPDAKW
EECCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCCHHHHHHEEECCCCCC
GETPCAFVELKEGREATSEELVAWCRERLAPYKLPRHVVFGELPKTSTGKVQKFVLREKA
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCCHHHHHHHHHHHH
REE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]