Click here to switch to the map view.

The map label for this gene is mchE [H]

Identifier: 218532327

GI number: 218532327

Start: 4698041

End: 4699330

Strand: Reverse

Name: mchE [H]

Synonym: Mchl_4439

Alternate gene names: 218532327

Gene position: 4699330-4698041 (Counterclockwise)

Preceding gene: 218532332

Following gene: 218532326

Centisome position: 81.33

GC content: 71.78

Gene sequence:

>1290_bases
GTGAACGCACCCCTGTTCCGTCGCGAGGTCGTGGAGGCACGGCGAAGCGCCTGGCTCGGCGAGGCCCAGGTGATCCAGCC
CCTGGCAATCCGCGTCGTGGTCGGTCTGTGCCTCGCCTTCGCCGTCCTCGTCGGCGTCTACGCCTGGCTCGGCAGCTACA
CCCGCCGGGTCCATGCCGACGGCCTGCTCGCGCCCGATATCGGCCTCATCACCGTGGCGAGCCCGCTGGCGGGGCGGGTC
AGCGCGTCGGGCGCCAAGGAGGGGGACCGGGTCGAGCGCGGCCAGCTCCTCTTCACCATCGACCTCGACGCGGTCTCGGC
GAGCGGCCCGACCCAGGAGCGGGTGATCGCCCAGCTCGGCCGTCAGCGCGAGAGCGTGGAGCGCCAGCGCGCCGCCCGCG
CGGCCATGGCCACCGCCGAGAAGGAGGGCTTGAGGGAGCAGGTCGCCAACCTGGAGGCACAGCGCGCCAGGATCGAGGAG
CAGCTCGCGCTCCAAGAGAAGCTGGTGCCGCCGCTCAAATCCCGCGCCGACGAACTCGCCGACCTTGTGACCCGCGGTTT
TGCCCGCGCGGCCGACTTCCAGAGCCAGAACTACCTCTATCTCCAGGCCAGTTCGCAGCTCGCGCAGTTCCGCCAGGGCG
GGCTTCAGGTCGAGGGCAAGCTCGGCGATCTGCGCGCCAAGCTCGCCACCTTCGACGAGACCCTGGCGCGCGATCTCGCC
GAACTCGACCGCACCGCCGCGCAGCTCGAACAGCAGCGGGCCGAGAGCGAGGCGCGCCGCGCCATCGAGGTGCGCGCGCC
GGAGCGCGGCATCCTCACCTCGATCCGGGCGCAGGCCGGGCAGACCGTGGCGGCGGGGGCGAACCTGCTCACCCTGCTGC
CGAGCGAGGGCCGGCTGCAGGCCAACCTGTTCGTCGATTCCTCGGCGATCGGCTTCATCGAGCCCGGCGCCAGCGTGATG
CTGCGCTACGCCGCCTTCCCGTTCCAGCGCTTCGGCCTGCACCGGGGCACGGTGACGGAGGTGACCCGCGCGCCGCTCGA
AGGCGATGCCCGGCCCGAGGCGGCGCGGGACACGGCCAAAAATGCGGGGAATGGGGGCGTCTACCGCATCATCGTGCGGC
CGGATGCCGACAGCGTGACGGCCTATGGCGAGCAACGCCGGCTGGAGGCCGGAATGCGGGTCGAGGCCGACATCGCGCTC
GAGAAGCGCCCGCTCTACCGCTGGCTCCTCGACCCCCTCTACCACGTCAAGCGCAGCGTCGATCTCGTCACCGAAGGAGG
GACACGTTGA

Upstream 100 bases:

>100_bases
CCATCACCGCTAAGAATCTGAAGCTGTTGCGGATTACGGCCTGCATGCGTCAGAACAACGCACCTAATCGTTCGATCGCT
ACCCCGGAGGAAGCTTCCCG

Downstream 100 bases:

>100_bases
GCCTGCTGTCGGATCTTCAGTTCGGTTTCCGGCGGCGCCTGCCGGTCGTGCTCCAGGCGGAGGCCGCCGAGTGCGGCATG
GCCTGCCTCGCCATGGTGCT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNAPLFRREVVEARRSAWLGEAQVIQPLAIRVVVGLCLAFAVLVGVYAWLGSYTRRVHADGLLAPDIGLITVASPLAGRV
SASGAKEGDRVERGQLLFTIDLDAVSASGPTQERVIAQLGRQRESVERQRAARAAMATAEKEGLREQVANLEAQRARIEE
QLALQEKLVPPLKSRADELADLVTRGFARAADFQSQNYLYLQASSQLAQFRQGGLQVEGKLGDLRAKLATFDETLARDLA
ELDRTAAQLEQQRAESEARRAIEVRAPERGILTSIRAQAGQTVAAGANLLTLLPSEGRLQANLFVDSSAIGFIEPGASVM
LRYAAFPFQRFGLHRGTVTEVTRAPLEGDARPEAARDTAKNAGNGGVYRIIVRPDADSVTAYGEQRRLEAGMRVEADIAL
EKRPLYRWLLDPLYHVKRSVDLVTEGGTR

Sequences:

>Translated_429_residues
MNAPLFRREVVEARRSAWLGEAQVIQPLAIRVVVGLCLAFAVLVGVYAWLGSYTRRVHADGLLAPDIGLITVASPLAGRV
SASGAKEGDRVERGQLLFTIDLDAVSASGPTQERVIAQLGRQRESVERQRAARAAMATAEKEGLREQVANLEAQRARIEE
QLALQEKLVPPLKSRADELADLVTRGFARAADFQSQNYLYLQASSQLAQFRQGGLQVEGKLGDLRAKLATFDETLARDLA
ELDRTAAQLEQQRAESEARRAIEVRAPERGILTSIRAQAGQTVAAGANLLTLLPSEGRLQANLFVDSSAIGFIEPGASVM
LRYAAFPFQRFGLHRGTVTEVTRAPLEGDARPEAARDTAKNAGNGGVYRIIVRPDADSVTAYGEQRRLEAGMRVEADIAL
EKRPLYRWLLDPLYHVKRSVDLVTEGGTR
>Mature_429_residues
MNAPLFRREVVEARRSAWLGEAQVIQPLAIRVVVGLCLAFAVLVGVYAWLGSYTRRVHADGLLAPDIGLITVASPLAGRV
SASGAKEGDRVERGQLLFTIDLDAVSASGPTQERVIAQLGRQRESVERQRAARAAMATAEKEGLREQVANLEAQRARIEE
QLALQEKLVPPLKSRADELADLVTRGFARAADFQSQNYLYLQASSQLAQFRQGGLQVEGKLGDLRAKLATFDETLARDLA
ELDRTAAQLEQQRAESEARRAIEVRAPERGILTSIRAQAGQTVAAGANLLTLLPSEGRLQANLFVDSSAIGFIEPGASVM
LRYAAFPFQRFGLHRGTVTEVTRAPLEGDARPEAARDTAKNAGNGGVYRIIVRPDADSVTAYGEQRRLEAGMRVEADIAL
EKRPLYRWLLDPLYHVKRSVDLVTEGGTR

Specific function: Probably involved, in conjunction with mchF, in the secretion of microcin H47 [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003997
- InterPro:   IPR006143
- InterPro:   IPR006144
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 46840; Mature: 46840

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00543 HLYD_FAMILY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPLFRREVVEARRSAWLGEAQVIQPLAIRVVVGLCLAFAVLVGVYAWLGSYTRRVHAD
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLLAPDIGLITVASPLAGRVSASGAKEGDRVERGQLLFTIDLDAVSASGPTQERVIAQLG
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHH
RQRESVERQRAARAAMATAEKEGLREQVANLEAQRARIEEQLALQEKLVPPLKSRADELA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
DLVTRGFARAADFQSQNYLYLQASSQLAQFRQGGLQVEGKLGDLRAKLATFDETLARDLA
HHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHH
ELDRTAAQLEQQRAESEARRAIEVRAPERGILTSIRAQAGQTVAAGANLLTLLPSEGRLQ
HHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCEECCCCCEEEECCCCCCEE
ANLFVDSSAIGFIEPGASVMLRYAAFPFQRFGLHRGTVTEVTRAPLEGDARPEAARDTAK
EEEEEECCCCCEECCCHHHHHHHHHCCHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHC
NAGNGGVYRIIVRPDADSVTAYGEQRRLEAGMRVEADIALEKRPLYRWLLDPLYHVKRSV
CCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECHHHCCCHHHHHHHHHHHHHHHHH
DLVTEGGTR
HHCCCCCCC
>Mature Secondary Structure
MNAPLFRREVVEARRSAWLGEAQVIQPLAIRVVVGLCLAFAVLVGVYAWLGSYTRRVHAD
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLLAPDIGLITVASPLAGRVSASGAKEGDRVERGQLLFTIDLDAVSASGPTQERVIAQLG
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHH
RQRESVERQRAARAAMATAEKEGLREQVANLEAQRARIEEQLALQEKLVPPLKSRADELA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
DLVTRGFARAADFQSQNYLYLQASSQLAQFRQGGLQVEGKLGDLRAKLATFDETLARDLA
HHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHH
ELDRTAAQLEQQRAESEARRAIEVRAPERGILTSIRAQAGQTVAAGANLLTLLPSEGRLQ
HHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCEECCCCCEEEECCCCCCEE
ANLFVDSSAIGFIEPGASVMLRYAAFPFQRFGLHRGTVTEVTRAPLEGDARPEAARDTAK
EEEEEECCCCCEECCCHHHHHHHHHCCHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHC
NAGNGGVYRIIVRPDADSVTAYGEQRRLEAGMRVEADIALEKRPLYRWLLDPLYHVKRSV
CCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECHHHCCCHHHHHHHHHHHHHHHHH
DLVTEGGTR
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11181394 [H]