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The map label for this gene is thcD [H]

Identifier: 218532258

GI number: 218532258

Start: 4611060

End: 4612322

Strand: Reverse

Name: thcD [H]

Synonym: Mchl_4370

Alternate gene names: 218532258

Gene position: 4612322-4611060 (Counterclockwise)

Preceding gene: 218532259

Following gene: 218532257

Centisome position: 79.83

GC content: 73.24

Gene sequence:

>1263_bases
GTGACGACACACCCTGAACGCATCGTCGTCGTCGGCGCCTCGCTGGCGGGCCTTGCCGCCGCCCATGCTCTGCGTGAACG
AGGCTATGCCGGCCGCCTGACCCTGGTCGGCGCCGAGCCCCACCGGCCCTACGACCGGCCGCCGCTCTCCAAGCAGGTGC
TGCGTGGGCTCTACTCCGCCGACACCACCCTGCCCTGCCATCCCGAGCTGCGCGCGGAGTTCCGCCTCGGGCAGCCGGCG
ACTCGGCTCGACCTGACCGGGCGCGTGGTCGAATGCGAGGACGGCAGCCGCCTGCCCTACGATCGCCTGCTGATCGCCAC
CGGCACCCGGGCGCGGCCCTGGCCCAACCCCGAGGAGGCCGCCCTCGCCGGTGTCTACACCCTGCGCGACCGCGACGACG
CCGAGCGGCTCGGCGCGCATCTGCGGACCAAGCCGCAACGCATCGTCCTCGTCGGCGGCGGCTTCATCGGCTTCGAGGTC
GCCTCCACCTGTCGCGATCTCGACATCCCCGTCACCCTGCTGGTGCGCGATGCCGTGCCGCTGGCGGCCGCGCTCGGTCC
CCGCCTCGGCGGCCTGATCGGCGAGGTCGCCCGCGAGAAGGGCGTCGATCTGCGTCTCGAATCCGAGATCGAGCGGATGG
AGGGGGAAGCGGGCCGCCTCGCCGCCGTGATCCTGAAGGACGGCACGCGGATCGAGACCGACTGCCTCGTGGCCGCCATC
GGCACTTTGCGCAATGTCGAGTGGCTCGACGGCGCGGGGCTTGAGGCCGACGCAGGGGGCCTGCGCTGCGACGCCTTCTG
CCGTGCGCTCCGCGCGGACGGCCGCGTCGCCGAGGGCGTTTTTTGCGCGGGCGACGTGGCGCGCTGGCCGAACCCGCACG
ACGACGACGCGCTGGTGGCGGTTGAGCATTGGGGCAACGCCCGGGCGCAAGCCGAGACCGCCGCCGCCAACATGCTGGCG
GGCGATGCCTCGGCGATGCGCCGCCACGACCACCTGCCCGATTTCTGGTCGCAGCAATTCGGCCTGACGATCAAGCTCGT
TGGCTTGACCGAGGGCGCCGACGCCCTCGCGGTGGTGCACGGCTCGTTCGAGGAGCGCCGCCTCGTGGCCGCCTTCGGCA
AGGCGGGCCGCACCGTCGCCGCCGCCGCCATCGACAGCGCCCGCTGGCTGCCCGCCTACAAGGCCGCGATCCGCGAGCGC
GCCCCATTCCCGCCGATCGAGGACGCCGTCGATCAGCCCCGCATCCGTGTGCAGGAGCCCTAG

Upstream 100 bases:

>100_bases
AGACCGCACGGGGCGAGATCGAGCGGGCCATCCATGCCTGCCCGGTGCGGGCGATCACCGCCTACCCCGATGCCGCCGAC
GCGGCGGCCGGAGAGGCGCG

Downstream 100 bases:

>100_bases
TCCCGTGAGCGGAAGCACACTGTTCGATCAGATCCTCGATCCCGCCAACCGGGCGAACCCCTACCCGCTCTACGCCGAGC
TGCGAAAAATCCCGGTCGCG

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTTHPERIVVVGASLAGLAAAHALRERGYAGRLTLVGAEPHRPYDRPPLSKQVLRGLYSADTTLPCHPELRAEFRLGQPA
TRLDLTGRVVECEDGSRLPYDRLLIATGTRARPWPNPEEAALAGVYTLRDRDDAERLGAHLRTKPQRIVLVGGGFIGFEV
ASTCRDLDIPVTLLVRDAVPLAAALGPRLGGLIGEVAREKGVDLRLESEIERMEGEAGRLAAVILKDGTRIETDCLVAAI
GTLRNVEWLDGAGLEADAGGLRCDAFCRALRADGRVAEGVFCAGDVARWPNPHDDDALVAVEHWGNARAQAETAAANMLA
GDASAMRRHDHLPDFWSQQFGLTIKLVGLTEGADALAVVHGSFEERRLVAAFGKAGRTVAAAAIDSARWLPAYKAAIRER
APFPPIEDAVDQPRIRVQEP

Sequences:

>Translated_420_residues
MTTHPERIVVVGASLAGLAAAHALRERGYAGRLTLVGAEPHRPYDRPPLSKQVLRGLYSADTTLPCHPELRAEFRLGQPA
TRLDLTGRVVECEDGSRLPYDRLLIATGTRARPWPNPEEAALAGVYTLRDRDDAERLGAHLRTKPQRIVLVGGGFIGFEV
ASTCRDLDIPVTLLVRDAVPLAAALGPRLGGLIGEVAREKGVDLRLESEIERMEGEAGRLAAVILKDGTRIETDCLVAAI
GTLRNVEWLDGAGLEADAGGLRCDAFCRALRADGRVAEGVFCAGDVARWPNPHDDDALVAVEHWGNARAQAETAAANMLA
GDASAMRRHDHLPDFWSQQFGLTIKLVGLTEGADALAVVHGSFEERRLVAAFGKAGRTVAAAAIDSARWLPAYKAAIRER
APFPPIEDAVDQPRIRVQEP
>Mature_419_residues
TTHPERIVVVGASLAGLAAAHALRERGYAGRLTLVGAEPHRPYDRPPLSKQVLRGLYSADTTLPCHPELRAEFRLGQPAT
RLDLTGRVVECEDGSRLPYDRLLIATGTRARPWPNPEEAALAGVYTLRDRDDAERLGAHLRTKPQRIVLVGGGFIGFEVA
STCRDLDIPVTLLVRDAVPLAAALGPRLGGLIGEVAREKGVDLRLESEIERMEGEAGRLAAVILKDGTRIETDCLVAAIG
TLRNVEWLDGAGLEADAGGLRCDAFCRALRADGRVAEGVFCAGDVARWPNPHDDDALVAVEHWGNARAQAETAAANMLAG
DASAMRRHDHLPDFWSQQFGLTIKLVGLTEGADALAVVHGSFEERRLVAAFGKAGRTVAAAAIDSARWLPAYKAAIRERA
PFPPIEDAVDQPRIRVQEP

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=384, Percent_Identity=29.1666666666667, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI65787454, Length=384, Percent_Identity=29.1666666666667, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI21389617, Length=384, Percent_Identity=29.1666666666667, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI22202629, Length=388, Percent_Identity=27.0618556701031, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI4757732, Length=388, Percent_Identity=27.0618556701031, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1788892, Length=401, Percent_Identity=29.426433915212, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789065, Length=284, Percent_Identity=27.112676056338, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI32564386, Length=397, Percent_Identity=25.9445843828715, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=390, Percent_Identity=25.6410256410256, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585130, Length=381, Percent_Identity=29.6587926509186, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24639257, Length=381, Percent_Identity=29.1338582677165, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI281359715, Length=381, Percent_Identity=29.1338582677165, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281359713, Length=381, Percent_Identity=29.1338582677165, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24639250, Length=381, Percent_Identity=29.1338582677165, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18543267, Length=381, Percent_Identity=29.1338582677165, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24639252, Length=381, Percent_Identity=29.1338582677165, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 45249; Mature: 45118

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHPERIVVVGASLAGLAAAHALRERGYAGRLTLVGAEPHRPYDRPPLSKQVLRGLYSA
CCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHC
DTTLPCHPELRAEFRLGQPATRLDLTGRVVECEDGSRLPYDRLLIATGTRARPWPNPEEA
CCCCCCCCCHHHHEECCCCCCEEECCCEEEECCCCCCCCHHEEEEEECCCCCCCCCCHHH
ALAGVYTLRDRDDAERLGAHLRTKPQRIVLVGGGFIGFEVASTCRDLDIPVTLLVRDAVP
HHEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCHH
LAAALGPRLGGLIGEVAREKGVDLRLESEIERMEGEAGRLAAVILKDGTRIETDCLVAAI
HHHHHCHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCEEEEEEECCCEECHHHHHHHH
GTLRNVEWLDGAGLEADAGGLRCDAFCRALRADGRVAEGVFCAGDVARWPNPHDDDALVA
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCHHCCCCCCCCCCEEE
VEHWGNARAQAETAAANMLAGDASAMRRHDHLPDFWSQQFGLTIKLVGLTEGADALAVVH
EECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCCEEEEEEECCCCCEEEEEE
GSFEERRLVAAFGKAGRTVAAAAIDSARWLPAYKAAIRERAPFPPIEDAVDQPRIRVQEP
CCHHHHHHHHHHCCCCCEEEEHHHHCHHCCHHHHHHHHHCCCCCCHHHHCCCCCEEECCC
>Mature Secondary Structure 
TTHPERIVVVGASLAGLAAAHALRERGYAGRLTLVGAEPHRPYDRPPLSKQVLRGLYSA
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHC
DTTLPCHPELRAEFRLGQPATRLDLTGRVVECEDGSRLPYDRLLIATGTRARPWPNPEEA
CCCCCCCCCHHHHEECCCCCCEEECCCEEEECCCCCCCCHHEEEEEECCCCCCCCCCHHH
ALAGVYTLRDRDDAERLGAHLRTKPQRIVLVGGGFIGFEVASTCRDLDIPVTLLVRDAVP
HHEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCHH
LAAALGPRLGGLIGEVAREKGVDLRLESEIERMEGEAGRLAAVILKDGTRIETDCLVAAI
HHHHHCHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCEEEEEEECCCEECHHHHHHHH
GTLRNVEWLDGAGLEADAGGLRCDAFCRALRADGRVAEGVFCAGDVARWPNPHDDDALVA
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCHHCCCCCCCCCCEEE
VEHWGNARAQAETAAANMLAGDASAMRRHDHLPDFWSQQFGLTIKLVGLTEGADALAVVH
EECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCCEEEEEEECCCCCEEEEEE
GSFEERRLVAAFGKAGRTVAAAAIDSARWLPAYKAAIRERAPFPPIEDAVDQPRIRVQEP
CCHHHHHHHHHHCCCCCEEEEHHHHCHHCCHHHHHHHHHCCCCCCHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]