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The map label for this gene is arsB [H]

Identifier: 218532222

GI number: 218532222

Start: 4576525

End: 4577793

Strand: Reverse

Name: arsB [H]

Synonym: Mchl_4334

Alternate gene names: 218532222

Gene position: 4577793-4576525 (Counterclockwise)

Preceding gene: 218532224

Following gene: 218532221

Centisome position: 79.23

GC content: 71.79

Gene sequence:

>1269_bases
ATGGGTGCGCTGATCCCGAACCCGAACGCGGCGACCTGGGGTATCGCCGCGCTGGCGACGCTCGGCGTGATCCTGCGCCC
GTTCTCCTGGCCTGAAGCGATCTGGGCGGTGCTCGGCGCGGCGCTCCTCGTCCTCCTCGGCCTGATCCCCTGGCAGAATG
CCCTGGAGGGCGCAGCCAAGGGGACCGACGTCTACCTCTTCCTCGTGGGGATGATGCTGCTCTCCGAGATCGCCCGGAAG
CAGGGCCTGTTCGATTGGCTCGCCGCCCACGCGGTGCGGGCCGCCAAGGGGTCGCCGACGCGGCTGTTTGCGCTCGTCTA
CGTCGTCGGCACGGTGGTTACGGTCTTCCTCTCGAACGATGCCTGCGCGGTGGTGCTGACGCCCGCCGTCTTCGCCGCGA
CGCGGGCCGCCGGGGTGAAGCAGCCCCTGCCCTACCTGTTCGTCTGCGCCTTCATCGCCAACGCGGCGAGCTTCGTGCTG
CCGATCTCGAACCCGGCCAACCTCGTCGTCTTCGCCGAGCACATGCCGCCGCTCGGCCGCTGGCTGGCGACCTTCACCCT
GCCCTCCCTCCTCGCCATCGTCGCGACCTATCTCGTCCTGCGCCAGACCCAGAACGCGCGGCTGAAGGCCGAGACGGTCG
CGACCGACGTCGCAATCCCGAGGCTCGAGCTCGGCGGCACGATCGCGGCCGGGGGCATCGTCGCCACCGGCGCGGCCCTG
ATCGGCGCCTCGGCCGCCGGGATCGAACTCGGTCTTCCGACCTTCATCGCCGGACTCGCCACCACCCTCGTCGTGCTCGC
GATCAATCGGGGCGGGCTGGTCGCGGTCGCTCGGGACGTCTCCTGGGGCGTGCTGCCTCTGGTCGCCGGGCTCTTCGTCC
TCGTCGAGTCCCTGGAGAAGACCGGCCTCCTCGCAAGGCTCGCCGACCTCCTGGGCCGCGCCGCGCAGGGCGATCCCGCC
GCGACGGCTTGGGCCGGCGGCGTGCTCGTCGCGTTCGGATCGAACCTCGTGAACAACCTGCCGGCGGGCCTCCTGGCGGG
CGCCGCGGTGCAGGCCGCCCATGTGCCGGAGACGGTGGCGGGGGCGATCCTGATCGGCGTCGATCTCGGGCCGAACCTCT
CGGTCACGGGCTCGCTCGCCACGATCCTCTGGCTCACCGCGATCCGCCGCGAGGGCCAGAACGTCTCCGCCTGGGCGTTC
CTGAAGCTCGGCGCCCTCGTCATGCCCCCGGCGCTGGCGCTGGCCCTCGCGGCTCTGATCCTCGCCTGA

Upstream 100 bases:

>100_bases
GAGGCCGTGCGCGATCCTGCTCCAAGCCGGAGCGACCCGCAGCGGTGCCCCGCCTCTCCGAATCCCGTGATCCTCCCCGA
CAGAAGGAAGCCCGACGCCG

Downstream 100 bases:

>100_bases
TCCCATACTCCTCGCCGATCCGGAGGCCGACCCGCGTGAGTGCCGCCCTGCTCTATCCCTTCATCATCGTGGCGGGCGCG
CTCCAGGCGCTCGGCAACTC

Product: Arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MGALIPNPNAATWGIAALATLGVILRPFSWPEAIWAVLGAALLVLLGLIPWQNALEGAAKGTDVYLFLVGMMLLSEIARK
QGLFDWLAAHAVRAAKGSPTRLFALVYVVGTVVTVFLSNDACAVVLTPAVFAATRAAGVKQPLPYLFVCAFIANAASFVL
PISNPANLVVFAEHMPPLGRWLATFTLPSLLAIVATYLVLRQTQNARLKAETVATDVAIPRLELGGTIAAGGIVATGAAL
IGASAAGIELGLPTFIAGLATTLVVLAINRGGLVAVARDVSWGVLPLVAGLFVLVESLEKTGLLARLADLLGRAAQGDPA
ATAWAGGVLVAFGSNLVNNLPAGLLAGAAVQAAHVPETVAGAILIGVDLGPNLSVTGSLATILWLTAIRREGQNVSAWAF
LKLGALVMPPALALALAALILA

Sequences:

>Translated_422_residues
MGALIPNPNAATWGIAALATLGVILRPFSWPEAIWAVLGAALLVLLGLIPWQNALEGAAKGTDVYLFLVGMMLLSEIARK
QGLFDWLAAHAVRAAKGSPTRLFALVYVVGTVVTVFLSNDACAVVLTPAVFAATRAAGVKQPLPYLFVCAFIANAASFVL
PISNPANLVVFAEHMPPLGRWLATFTLPSLLAIVATYLVLRQTQNARLKAETVATDVAIPRLELGGTIAAGGIVATGAAL
IGASAAGIELGLPTFIAGLATTLVVLAINRGGLVAVARDVSWGVLPLVAGLFVLVESLEKTGLLARLADLLGRAAQGDPA
ATAWAGGVLVAFGSNLVNNLPAGLLAGAAVQAAHVPETVAGAILIGVDLGPNLSVTGSLATILWLTAIRREGQNVSAWAF
LKLGALVMPPALALALAALILA
>Mature_421_residues
GALIPNPNAATWGIAALATLGVILRPFSWPEAIWAVLGAALLVLLGLIPWQNALEGAAKGTDVYLFLVGMMLLSEIARKQ
GLFDWLAAHAVRAAKGSPTRLFALVYVVGTVVTVFLSNDACAVVLTPAVFAATRAAGVKQPLPYLFVCAFIANAASFVLP
ISNPANLVVFAEHMPPLGRWLATFTLPSLLAIVATYLVLRQTQNARLKAETVATDVAIPRLELGGTIAAGGIVATGAALI
GASAAGIELGLPTFIAGLATTLVVLAINRGGLVAVARDVSWGVLPLVAGLFVLVESLEKTGLLARLADLLGRAAQGDPAA
TAWAGGVLVAFGSNLVNNLPAGLLAGAAVQAAHVPETVAGAILIGVDLGPNLSVTGSLATILWLTAIRREGQNVSAWAFL
KLGALVMPPALALALAALILA

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=370, Percent_Identity=27.8378378378378, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 43082; Mature: 42950

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGALIPNPNAATWGIAALATLGVILRPFSWPEAIWAVLGAALLVLLGLIPWQNALEGAAK
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
GTDVYLFLVGMMLLSEIARKQGLFDWLAAHAVRAAKGSPTRLFALVYVVGTVVTVFLSND
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
ACAVVLTPAVFAATRAAGVKQPLPYLFVCAFIANAASFVLPISNPANLVVFAEHMPPLGR
CEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHEEEECCCCCCEEEEECCCCCHHH
WLATFTLPSLLAIVATYLVLRQTQNARLKAETVATDVAIPRLELGGTIAAGGIVATGAAL
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCCEEECCHHHHHHHHH
IGASAAGIELGLPTFIAGLATTLVVLAINRGGLVAVARDVSWGVLPLVAGLFVLVESLEK
HCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
TGLLARLADLLGRAAQGDPAATAWAGGVLVAFGSNLVNNLPAGLLAGAAVQAAHVPETVA
HHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHH
GAILIGVDLGPNLSVTGSLATILWLTAIRREGQNVSAWAFLKLGALVMPPALALALAALI
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LA
HC
>Mature Secondary Structure 
GALIPNPNAATWGIAALATLGVILRPFSWPEAIWAVLGAALLVLLGLIPWQNALEGAAK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
GTDVYLFLVGMMLLSEIARKQGLFDWLAAHAVRAAKGSPTRLFALVYVVGTVVTVFLSND
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
ACAVVLTPAVFAATRAAGVKQPLPYLFVCAFIANAASFVLPISNPANLVVFAEHMPPLGR
CEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHEEEECCCCCCEEEEECCCCCHHH
WLATFTLPSLLAIVATYLVLRQTQNARLKAETVATDVAIPRLELGGTIAAGGIVATGAAL
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCCEEECCHHHHHHHHH
IGASAAGIELGLPTFIAGLATTLVVLAINRGGLVAVARDVSWGVLPLVAGLFVLVESLEK
HCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
TGLLARLADLLGRAAQGDPAATAWAGGVLVAFGSNLVNNLPAGLLAGAAVQAAHVPETVA
HHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHH
GAILIGVDLGPNLSVTGSLATILWLTAIRREGQNVSAWAFLKLGALVMPPALALALAALI
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LA
HC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA