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The map label for this gene is aspA [H]

Identifier: 218532214

GI number: 218532214

Start: 4566306

End: 4568012

Strand: Reverse

Name: aspA [H]

Synonym: Mchl_4326

Alternate gene names: 218532214

Gene position: 4568012-4566306 (Counterclockwise)

Preceding gene: 218532215

Following gene: 218532209

Centisome position: 79.06

GC content: 68.42

Gene sequence:

>1707_bases
ATGGCATCCGACACCAAGGCCAAGCGCTCCGCCAAGGATGCGGCCGGAGGCCAGGGTGAGGTCAAGAAGTCCAAGGCGGC
GGCCGGGAAGGCCAAGGGCACGGGCGGCAAAGGCAGCACCGGAAAGAAGGCGGCCAAGGCCGAGATGAAGGCTGCGGCCA
TCGCGGCGACCGAGAAGGCCGCCCGCCCGGACGCGCTCGGGACGGTGGCAACGACCACGGCGGAGAATCCGCCCCAGGAG
GAGGATCTGGCTGTCGCCACCGTGCGCAAGGTGGCGATGGAAGGGGCGAAGACCCGCTCGGAACACGACCTGCTCGGGGA
CGGCGAGGTGCCGGCCGATGCGCTGTGGGGCATCCACACCAAGCGAGCGGTGGCGAACTTCCCGATCACCGGCGTGTCGG
TGGGCCATTATCCGGAGCTGGTGCGGGCGCTCGCCCTGGTGAAACAGGCCGCGGCGCGGTCCAACCACCGGCTCGGCTAC
CTGCCGAAGGTGAAGGCAAAGATCATCGAGGAGGCCTGCATCCTCGTCGCGACCGATCCGGCTTACGCCGAATCCTTCGT
GGTCGATGCGATCCAGGGCGGGGCCGGCACCTCGACCAACATGAACGCCAACGAGGTCATCGCCAATGTCGGCTTGAGGC
TGATGGGCAAGGCGCCGGGCGACTACGCCGCGCTGCATCCCAACGACGACGTCAACATGGCGCAATCGACCAACGACGCC
TACCCGACGGCCCTGCGGCTCGCGGTGATCTTCGCCACCCAGCCGCTGGTCAAGGCGCTCGACGACCTCGCCTACGCCTT
CAAGAGCAAGGCGGTGGAGTTCGCCGACGTGCTCAAGATGGGCCGCACCCAGCTCCAGGACGCGGTACCGATGACGCTCG
GCCAGGAGTTCGACGGCTTCCACGCCACGATCAAGGAGGACGTGGCGCGATTGACCGAGATCGTCGGCCTGTTCCGCGAG
GTGAATCTCGGCGCCACCGCGATCGGCACCGGGATCAACGCCGACCCGCGCTACGCCGCGCTGGCCGTGGAGGAGCTGTC
GCGGCTCTCGGGCCAGCCGATGGTGCTGGCCTCGAACCTGATCGAGGCGACCTCGGATCTCGGCGCCTTCGTGCTGTTCT
CCGGCGTCCTGAAGCGCGTCGCGGTCAAGCTGTCCAAGATCTGCAACGATCTGCGCCTGCTCTCGTCCGGTCCCCGCACC
GGTTTCGGCGAGATCCGGCTGCCGGCGGTGCAGGCGGGCTCCTCGATCATGCCGGGCAAGGTCAACCCGGTGATCCCGGA
AGTGGTCAACCAAGTCGCCTACATGGTGATCGGCCACGACCTGACGGTGACGCTCTGCGCCGAGGGCGGCCAGCTCCAGC
TCAACGCCTTCGAGCCGACCATCGGCTACTGCGTGCTGAGCTCCTTACGGATGCTGACGGCCGCCATCGACACCCTGACC
AAGCGCTGCATCGACGGCATCGAGGCGGACCGCGAGCGCTGCCGCAGCCTCGTCCAGGGCTCGATCGGCCTCGTTACGGC
GCTCGCGCCGACGCTGGGCTACGAGGCCAGTTCCCGCATCGCCCGCCGCGCGCTGAAGGAGAACCGCGCGGTGGCGGATC
TGGTGCTGGAGGAGGGCCTCCTCACCGAGGCCCAGCTCAACGGACTGCTCGAACTGGAAGCCATGACCCACCCGACCCGG
CGGCAGAAGGCCGGGACGGTCGTGTGA

Upstream 100 bases:

>100_bases
GCACGCGGAGACCTGAACACCGACCCGACGGTCACCGGCCGCTGACATCGTGGATTCGGCCGGATCGTCCGACGCATCGG
GGGAGACTTCCGTGGCGAAG

Downstream 100 bases:

>100_bases
GTCTCTGGGGGTGAGCCTTGCGGCGGTCAGGGGATGACCCGCTCCGCCCGCAGCCGCACGAACAGGTCGAAGAACGCGTC
GTCCGAGGGCTGGTAATCGA

Product: fumarate lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MASDTKAKRSAKDAAGGQGEVKKSKAAAGKAKGTGGKGSTGKKAAKAEMKAAAIAATEKAARPDALGTVATTTAENPPQE
EDLAVATVRKVAMEGAKTRSEHDLLGDGEVPADALWGIHTKRAVANFPITGVSVGHYPELVRALALVKQAAARSNHRLGY
LPKVKAKIIEEACILVATDPAYAESFVVDAIQGGAGTSTNMNANEVIANVGLRLMGKAPGDYAALHPNDDVNMAQSTNDA
YPTALRLAVIFATQPLVKALDDLAYAFKSKAVEFADVLKMGRTQLQDAVPMTLGQEFDGFHATIKEDVARLTEIVGLFRE
VNLGATAIGTGINADPRYAALAVEELSRLSGQPMVLASNLIEATSDLGAFVLFSGVLKRVAVKLSKICNDLRLLSSGPRT
GFGEIRLPAVQAGSSIMPGKVNPVIPEVVNQVAYMVIGHDLTVTLCAEGGQLQLNAFEPTIGYCVLSSLRMLTAAIDTLT
KRCIDGIEADRERCRSLVQGSIGLVTALAPTLGYEASSRIARRALKENRAVADLVLEEGLLTEAQLNGLLELEAMTHPTR
RQKAGTVV

Sequences:

>Translated_568_residues
MASDTKAKRSAKDAAGGQGEVKKSKAAAGKAKGTGGKGSTGKKAAKAEMKAAAIAATEKAARPDALGTVATTTAENPPQE
EDLAVATVRKVAMEGAKTRSEHDLLGDGEVPADALWGIHTKRAVANFPITGVSVGHYPELVRALALVKQAAARSNHRLGY
LPKVKAKIIEEACILVATDPAYAESFVVDAIQGGAGTSTNMNANEVIANVGLRLMGKAPGDYAALHPNDDVNMAQSTNDA
YPTALRLAVIFATQPLVKALDDLAYAFKSKAVEFADVLKMGRTQLQDAVPMTLGQEFDGFHATIKEDVARLTEIVGLFRE
VNLGATAIGTGINADPRYAALAVEELSRLSGQPMVLASNLIEATSDLGAFVLFSGVLKRVAVKLSKICNDLRLLSSGPRT
GFGEIRLPAVQAGSSIMPGKVNPVIPEVVNQVAYMVIGHDLTVTLCAEGGQLQLNAFEPTIGYCVLSSLRMLTAAIDTLT
KRCIDGIEADRERCRSLVQGSIGLVTALAPTLGYEASSRIARRALKENRAVADLVLEEGLLTEAQLNGLLELEAMTHPTR
RQKAGTVV
>Mature_567_residues
ASDTKAKRSAKDAAGGQGEVKKSKAAAGKAKGTGGKGSTGKKAAKAEMKAAAIAATEKAARPDALGTVATTTAENPPQEE
DLAVATVRKVAMEGAKTRSEHDLLGDGEVPADALWGIHTKRAVANFPITGVSVGHYPELVRALALVKQAAARSNHRLGYL
PKVKAKIIEEACILVATDPAYAESFVVDAIQGGAGTSTNMNANEVIANVGLRLMGKAPGDYAALHPNDDVNMAQSTNDAY
PTALRLAVIFATQPLVKALDDLAYAFKSKAVEFADVLKMGRTQLQDAVPMTLGQEFDGFHATIKEDVARLTEIVGLFREV
NLGATAIGTGINADPRYAALAVEELSRLSGQPMVLASNLIEATSDLGAFVLFSGVLKRVAVKLSKICNDLRLLSSGPRTG
FGEIRLPAVQAGSSIMPGKVNPVIPEVVNQVAYMVIGHDLTVTLCAEGGQLQLNAFEPTIGYCVLSSLRMLTAAIDTLTK
RCIDGIEADRERCRSLVQGSIGLVTALAPTLGYEASSRIARRALKENRAVADLVLEEGLLTEAQLNGLLELEAMTHPTRR
QKAGTVV

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=39.2624728850325, Blast_Score=314, Evalue=2e-85,
Organism=Escherichia coli, GI87082375, Length=468, Percent_Identity=53.6324786324786, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=40.5228758169935, Blast_Score=333, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17553882, Length=464, Percent_Identity=39.4396551724138, Blast_Score=321, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=40.0611620795107, Blast_Score=239, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=38.3116883116883, Blast_Score=315, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24640179, Length=470, Percent_Identity=38.936170212766, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24640177, Length=479, Percent_Identity=38.6221294363257, Blast_Score=329, Evalue=4e-90,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=39.3939393939394, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=37.5, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=36.0691144708423, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 59682; Mature: 59551

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDTKAKRSAKDAAGGQGEVKKSKAAAGKAKGTGGKGSTGKKAAKAEMKAAAIAATEKA
CCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ARPDALGTVATTTAENPPQEEDLAVATVRKVAMEGAKTRSEHDLLGDGEVPADALWGIHT
CCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHH
KRAVANFPITGVSVGHYPELVRALALVKQAAARSNHRLGYLPKVKAKIIEEACILVATDP
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCEEEEECCH
AYAESFVVDAIQGGAGTSTNMNANEVIANVGLRLMGKAPGDYAALHPNDDVNMAQSTNDA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHEECCCCCCCEEECCCCCCCHHCCCCCC
YPTALRLAVIFATQPLVKALDDLAYAFKSKAVEFADVLKMGRTQLQDAVPMTLGQEFDGF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCH
HATIKEDVARLTEIVGLFREVNLGATAIGTGINADPRYAALAVEELSRLSGQPMVLASNL
HHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHH
IEATSDLGAFVLFSGVLKRVAVKLSKICNDLRLLSSGPRTGFGEIRLPAVQAGSSIMPGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHCCCCCCCCC
VNPVIPEVVNQVAYMVIGHDLTVTLCAEGGQLQLNAFEPTIGYCVLSSLRMLTAAIDTLT
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
KRCIDGIEADRERCRSLVQGSIGLVTALAPTLGYEASSRIARRALKENRAVADLVLEEGL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHCCC
LTEAQLNGLLELEAMTHPTRRQKAGTVV
CCHHHHCCEEEEHHHCCCHHHCCCCCCC
>Mature Secondary Structure 
ASDTKAKRSAKDAAGGQGEVKKSKAAAGKAKGTGGKGSTGKKAAKAEMKAAAIAATEKA
CCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ARPDALGTVATTTAENPPQEEDLAVATVRKVAMEGAKTRSEHDLLGDGEVPADALWGIHT
CCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHH
KRAVANFPITGVSVGHYPELVRALALVKQAAARSNHRLGYLPKVKAKIIEEACILVATDP
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCEEEEECCH
AYAESFVVDAIQGGAGTSTNMNANEVIANVGLRLMGKAPGDYAALHPNDDVNMAQSTNDA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHEECCCCCCCEEECCCCCCCHHCCCCCC
YPTALRLAVIFATQPLVKALDDLAYAFKSKAVEFADVLKMGRTQLQDAVPMTLGQEFDGF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCH
HATIKEDVARLTEIVGLFREVNLGATAIGTGINADPRYAALAVEELSRLSGQPMVLASNL
HHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHH
IEATSDLGAFVLFSGVLKRVAVKLSKICNDLRLLSSGPRTGFGEIRLPAVQAGSSIMPGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHCCCCCCCCC
VNPVIPEVVNQVAYMVIGHDLTVTLCAEGGQLQLNAFEPTIGYCVLSSLRMLTAAIDTLT
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
KRCIDGIEADRERCRSLVQGSIGLVTALAPTLGYEASSRIARRALKENRAVADLVLEEGL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHCCC
LTEAQLNGLLELEAMTHPTRRQKAGTVV
CCHHHHCCEEEEHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]