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The map label for this gene is dnaB [H]

Identifier: 218532194

GI number: 218532194

Start: 4547966

End: 4549453

Strand: Reverse

Name: dnaB [H]

Synonym: Mchl_4306

Alternate gene names: 218532194

Gene position: 4549453-4547966 (Counterclockwise)

Preceding gene: 218532195

Following gene: 218532193

Centisome position: 78.74

GC content: 65.86

Gene sequence:

>1488_bases
ATGGCCCTGCCCAACGCCCTCACGGCCAATCTCGAGACGGTGCAGCCCGAATATCGGGTGCCGCCGCACAACATCGACGC
GGAGCAGGCCCTGCTCGGCGCGATCATGGTGAACAACGACGCCTATTACCGTGTCTCGGACTTCCTGCTGCCCGAGCACT
TCATGGAGGCGGTGCACCGCCAGATCTTCGAGGTGTCGGTCTCGCTGATCAAGGCGGGCAAGCTCGCCACGCCGATCACG
CTGAAGACCTATCTCGGCGACATCGATCTCGGCGGCGTTACGCCGATGCAGTACCTCGCCCGCCTCGCGGCCGAGGCGAC
CACCGTCATCAACGCGGGCGAGTACGGCCGCACCATCTACGATCTGGCCATCCGCCGCCGGCTCATCACCATCGGCGAGG
ATCTGGTCAACGGCGCGTTCGAGGCGCCCGTCGAATCCCGGCCGCGCGACCAGATCGAGGCGACCGAGCGCCGGCTCTAC
GAACTCGCCGAATCCGGCAAGTACGACGGCGGCTTCCAGAAATTCTCCGACGCGCTGACCGCGGCCATCGACATGGCGGC
CAAAGCCTACCAGCGCGACGGCAAGCTCTCGGGCATCTCCACGGGGCTCACCGACCTCGACGCCAAGATGGGCGGCCTGC
AGCCCTCCGACCTCATCATCCTCGCAGGGCGCCCGGCCATGGGCAAGACCTCGCTCGTGACCAACATCGCCTTCAACATC
GCCAAGGCCTATCGCGGCGAGAAGCAGCCCGACGGCACCATGCAGACCGTCAACGGCGGCATCGTCGGCTTCTTCTCGCT
CGAAATGTCGGCCGAGCAATTGGCGACCCGTATCATCGCCGAGCAGTCCGGCGTGCCCTCCTACAAGATCCGCCGCGGCG
ACATCCGCCCGGAGGACTTCTACAAGATCACCGACGCCGCCCGGGACATGCAGACGATCCCGTTCTACATCGACCAGACC
GGCGGCATCTCGATCGCCCAGCTCGCCGCCCGTGCGCGCCGCCTCAAGCGCCAGAAGGGTCTCGACCTGCTCATCATCGA
CTACCTTCAGCTCCTCTCCGGCTCCGGCAAGAAGAGCGACAACCGCGTGCAGGAGATGACCGAGATCACCACCGGCATGA
AGGCTTTGGCCAAGGAGCTGGCGGTGCCGATCATCGGCCTGTCGCAGCTCTCGCGTCAGGTCGAGAACCGCGACGACAAG
CGGCCCCAGCTCTCGGACCTGCGCGAATCCGGCTCGATCGAGCAGGACGCCGACGTGGTGATGTTCGTGTTCCGCGAGGA
GTACTACGTCGGCAACAAGAAGCCGCGCGAGGGCACCGAGGAGTTCTTCGCCTGGGAAGCCGAGATGCAGCGCGTCCACG
GCAAGGCCGAGGTCATCCTCGGCAAGCAGCGTCACGGCCCGACCGGCACGGTGGAGCTGCAGTTCGACGCCAACATCACC
CGGTTCTCGAACCTGGCGCAAAGCGACCGGATGCCCGAGCAGATGTGA

Upstream 100 bases:

>100_bases
GCCGGGTCGGCTAAGGAAGGCTTCACCATCCCGAGGCAAGGTCCGGCGGGCATGAAACGGGGCGGCCAAGCCGCCCGCAT
CGGAGCGACGGACAAGCGCC

Downstream 100 bases:

>100_bases
TATCGGTGGCCGGATCGACGGTCCGGTCCCATCTCCGCCGGGGCTTTCCCCGCAGGAGTCTGTCATAGCGCCCGTAGAAT
CCTTCCCGGAAAACCCGACC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MALPNALTANLETVQPEYRVPPHNIDAEQALLGAIMVNNDAYYRVSDFLLPEHFMEAVHRQIFEVSVSLIKAGKLATPIT
LKTYLGDIDLGGVTPMQYLARLAAEATTVINAGEYGRTIYDLAIRRRLITIGEDLVNGAFEAPVESRPRDQIEATERRLY
ELAESGKYDGGFQKFSDALTAAIDMAAKAYQRDGKLSGISTGLTDLDAKMGGLQPSDLIILAGRPAMGKTSLVTNIAFNI
AKAYRGEKQPDGTMQTVNGGIVGFFSLEMSAEQLATRIIAEQSGVPSYKIRRGDIRPEDFYKITDAARDMQTIPFYIDQT
GGISIAQLAARARRLKRQKGLDLLIIDYLQLLSGSGKKSDNRVQEMTEITTGMKALAKELAVPIIGLSQLSRQVENRDDK
RPQLSDLRESGSIEQDADVVMFVFREEYYVGNKKPREGTEEFFAWEAEMQRVHGKAEVILGKQRHGPTGTVELQFDANIT
RFSNLAQSDRMPEQM

Sequences:

>Translated_495_residues
MALPNALTANLETVQPEYRVPPHNIDAEQALLGAIMVNNDAYYRVSDFLLPEHFMEAVHRQIFEVSVSLIKAGKLATPIT
LKTYLGDIDLGGVTPMQYLARLAAEATTVINAGEYGRTIYDLAIRRRLITIGEDLVNGAFEAPVESRPRDQIEATERRLY
ELAESGKYDGGFQKFSDALTAAIDMAAKAYQRDGKLSGISTGLTDLDAKMGGLQPSDLIILAGRPAMGKTSLVTNIAFNI
AKAYRGEKQPDGTMQTVNGGIVGFFSLEMSAEQLATRIIAEQSGVPSYKIRRGDIRPEDFYKITDAARDMQTIPFYIDQT
GGISIAQLAARARRLKRQKGLDLLIIDYLQLLSGSGKKSDNRVQEMTEITTGMKALAKELAVPIIGLSQLSRQVENRDDK
RPQLSDLRESGSIEQDADVVMFVFREEYYVGNKKPREGTEEFFAWEAEMQRVHGKAEVILGKQRHGPTGTVELQFDANIT
RFSNLAQSDRMPEQM
>Mature_494_residues
ALPNALTANLETVQPEYRVPPHNIDAEQALLGAIMVNNDAYYRVSDFLLPEHFMEAVHRQIFEVSVSLIKAGKLATPITL
KTYLGDIDLGGVTPMQYLARLAAEATTVINAGEYGRTIYDLAIRRRLITIGEDLVNGAFEAPVESRPRDQIEATERRLYE
LAESGKYDGGFQKFSDALTAAIDMAAKAYQRDGKLSGISTGLTDLDAKMGGLQPSDLIILAGRPAMGKTSLVTNIAFNIA
KAYRGEKQPDGTMQTVNGGIVGFFSLEMSAEQLATRIIAEQSGVPSYKIRRGDIRPEDFYKITDAARDMQTIPFYIDQTG
GISIAQLAARARRLKRQKGLDLLIIDYLQLLSGSGKKSDNRVQEMTEITTGMKALAKELAVPIIGLSQLSRQVENRDDKR
PQLSDLRESGSIEQDADVVMFVFREEYYVGNKKPREGTEEFFAWEAEMQRVHGKAEVILGKQRHGPTGTVELQFDANITR
FSNLAQSDRMPEQM

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=477, Percent_Identity=37.9454926624738, Blast_Score=289, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54840; Mature: 54709

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPNALTANLETVQPEYRVPPHNIDAEQALLGAIMVNNDAYYRVSDFLLPEHFMEAVHR
CCCCCCHHCCHHHCCCCCCCCCCCCCHHHHHHHHEEECCCCEEEHHHHHCCHHHHHHHHH
QIFEVSVSLIKAGKLATPITLKTYLGDIDLGGVTPMQYLARLAAEATTVINAGEYGRTIY
HHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEECCHHCHHHH
DLAIRRRLITIGEDLVNGAFEAPVESRPRDQIEATERRLYELAESGKYDGGFQKFSDALT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AAIDMAAKAYQRDGKLSGISTGLTDLDAKMGGLQPSDLIILAGRPAMGKTSLVTNIAFNI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
AKAYRGEKQPDGTMQTVNGGIVGFFSLEMSAEQLATRIIAEQSGVPSYKIRRGDIRPEDF
HHHHCCCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
YKITDAARDMQTIPFYIDQTGGISIAQLAARARRLKRQKGLDLLIIDYLQLLSGSGKKSD
HHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCH
NRVQEMTEITTGMKALAKELAVPIIGLSQLSRQVENRDDKRPQLSDLRESGSIEQDADVV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEE
MFVFREEYYVGNKKPREGTEEFFAWEAEMQRVHGKAEVILGKQRHGPTGTVELQFDANIT
EEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCHH
RFSNLAQSDRMPEQM
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
ALPNALTANLETVQPEYRVPPHNIDAEQALLGAIMVNNDAYYRVSDFLLPEHFMEAVHR
CCCCCHHCCHHHCCCCCCCCCCCCCHHHHHHHHEEECCCCEEEHHHHHCCHHHHHHHHH
QIFEVSVSLIKAGKLATPITLKTYLGDIDLGGVTPMQYLARLAAEATTVINAGEYGRTIY
HHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEECCHHCHHHH
DLAIRRRLITIGEDLVNGAFEAPVESRPRDQIEATERRLYELAESGKYDGGFQKFSDALT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AAIDMAAKAYQRDGKLSGISTGLTDLDAKMGGLQPSDLIILAGRPAMGKTSLVTNIAFNI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
AKAYRGEKQPDGTMQTVNGGIVGFFSLEMSAEQLATRIIAEQSGVPSYKIRRGDIRPEDF
HHHHCCCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
YKITDAARDMQTIPFYIDQTGGISIAQLAARARRLKRQKGLDLLIIDYLQLLSGSGKKSD
HHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCH
NRVQEMTEITTGMKALAKELAVPIIGLSQLSRQVENRDDKRPQLSDLRESGSIEQDADVV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEE
MFVFREEYYVGNKKPREGTEEFFAWEAEMQRVHGKAEVILGKQRHGPTGTVELQFDANIT
EEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCHH
RFSNLAQSDRMPEQM
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA