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The map label for this gene is radA [H]

Identifier: 218532192

GI number: 218532192

Start: 4545167

End: 4546597

Strand: Reverse

Name: radA [H]

Synonym: Mchl_4304

Alternate gene names: 218532192

Gene position: 4546597-4545167 (Counterclockwise)

Preceding gene: 218532193

Following gene: 218532189

Centisome position: 78.69

GC content: 72.19

Gene sequence:

>1431_bases
ATGGCAAAAATCCAACAGACCTTCGTCTGCCAGTCCTGCGGGGCGGTCTACAACCGCTGGCGCGGGCGCTGCGAGGCCTG
CAACGGCTGGAACACGATCCAGGAGGAGGTCGCCTCCGCCGGCCCGCAATCGGGCCCGGCCGCGACCCGACCCTCGCGGG
CGCGGGGCCGCGTCTTCCCCCTCGAAGGCCTGACCGGCGAGGCCAAGGAGGCCCCGCGCACGCCCTCGGGCATCAACGAG
CTCGACCGCGTCACCGGCGGCGGCTTCGTGCGCGGCTCGGTGATCCTGCTCGGCGGGGACCCCGGCATCGGCAAGTCGAC
CCTGCTGATGCAGGCCTCCGCCGCGATGGCCAAGAGCGGGGAGCGGGTCGCCTACATCTCCGGCGAGGAGGCGGTGGGGC
AGGTGCGCCTGCGCGCCGAACGCCTCGGGCTGGCCAAGTATCCGGTGGAGCTGGCGGCGCAAACGAATGTCGAGGACATC
GTCGAGACGCTGTCGCAGGGCCACCCGCCGGCGCTCGTCATCATCGACTCGATCCAGACCATGTGGACCGAGACGGTGGA
ATCGGCGCCCGGCACCGTCACGCAGGTGCGCTCATCGGCCCAGGCGCTGATCCGTTTTGCCAAGACCACGGGCACGGCCG
TCATCCTCGTCGGCCACGTCACCAAGGACGGGCAGATCGCGGGCCCCCGCGTGGTCGAGCACATGGTCGATGCGGTCGCC
TCGTTCGAGGGCGACCAGGGCCACCATTTCCGAATCTTACGCGCTGTGAAGAACCGGTTCGGGCCGACCGACGAGATCGG
CGTGTTCGAGATGACCGATGCCGGCCTGTCCGAAGTCCCGAACCCCTCCGCTCTGTTCCTGGCCGGGCGCGACCACGCCG
CGCCGGGCACCGCCGTCTTTGCCGGAATGGAGGGCACGCGTCCGCTGCTGGTCGAGATCCAGGCGCTCGTCGCCCCCTCC
TCGCTCGGCATGCCGCGCCGCGCCGTGGTCGGCTGGGACCCCAACCGCCTGTCGATGGTGCTCGCCGTGCTGGAGGCCCA
TGGCGGCATCCGGCTCGGCGGCCACGACGTCTACCTCAACGTCGCCGGGGGCCTGCGCATCACCGAGCCCGCCGCGGATC
TCGCGGTCGCCGCCGCCCTCGTCTCGTCGCTCTCGGGCGCGGCGCTTCCCTCCGACTCGGTCTATTTCGGCGAACTCGGC
CTGTCGGGGGCGGTGCGGCCGGTCTCGCAGGCGCCGGCCCGGCTGAAGGAGGCGTTGAAGCTCGGCTTTGGCAAGGCGAT
CACCCCGCAAGGGCGCGGCGAGGCCGGGGACCGGGCGCTGCCGACGGATGCGCTGCGCCACATCGCCGACCTCGTCGCCG
GCATCGCCTCGGGCGCGCCGCGAAAGGGCGGGGGCCGGCCCCGGGCGGTGCGGTTCGAGGAGGAGATGTAG

Upstream 100 bases:

>100_bases
GCCGGCTGGAGCCCTCCTTCGAGGCGGCTTCGCAGCACCTCAGGGTGAGCGGGCTGGGTGGGAGAGGTTCGATAGATCCC
TGCCCTGAGCCGTCTCCCCC

Downstream 100 bases:

>100_bases
CGCGGCCTATTCCGGCGCGGCCCGCGGCGCGAGATGCGGCCGCAGGATCGTCAGCATCCGCTCGGCTGAGGTGCCGGGCG
GAAAGAAGCCGAGATAGGCG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAKIQQTFVCQSCGAVYNRWRGRCEACNGWNTIQEEVASAGPQSGPAATRPSRARGRVFPLEGLTGEAKEAPRTPSGINE
LDRVTGGGFVRGSVILLGGDPGIGKSTLLMQASAAMAKSGERVAYISGEEAVGQVRLRAERLGLAKYPVELAAQTNVEDI
VETLSQGHPPALVIIDSIQTMWTETVESAPGTVTQVRSSAQALIRFAKTTGTAVILVGHVTKDGQIAGPRVVEHMVDAVA
SFEGDQGHHFRILRAVKNRFGPTDEIGVFEMTDAGLSEVPNPSALFLAGRDHAAPGTAVFAGMEGTRPLLVEIQALVAPS
SLGMPRRAVVGWDPNRLSMVLAVLEAHGGIRLGGHDVYLNVAGGLRITEPAADLAVAAALVSSLSGAALPSDSVYFGELG
LSGAVRPVSQAPARLKEALKLGFGKAITPQGRGEAGDRALPTDALRHIADLVAGIASGAPRKGGGRPRAVRFEEEM

Sequences:

>Translated_476_residues
MAKIQQTFVCQSCGAVYNRWRGRCEACNGWNTIQEEVASAGPQSGPAATRPSRARGRVFPLEGLTGEAKEAPRTPSGINE
LDRVTGGGFVRGSVILLGGDPGIGKSTLLMQASAAMAKSGERVAYISGEEAVGQVRLRAERLGLAKYPVELAAQTNVEDI
VETLSQGHPPALVIIDSIQTMWTETVESAPGTVTQVRSSAQALIRFAKTTGTAVILVGHVTKDGQIAGPRVVEHMVDAVA
SFEGDQGHHFRILRAVKNRFGPTDEIGVFEMTDAGLSEVPNPSALFLAGRDHAAPGTAVFAGMEGTRPLLVEIQALVAPS
SLGMPRRAVVGWDPNRLSMVLAVLEAHGGIRLGGHDVYLNVAGGLRITEPAADLAVAAALVSSLSGAALPSDSVYFGELG
LSGAVRPVSQAPARLKEALKLGFGKAITPQGRGEAGDRALPTDALRHIADLVAGIASGAPRKGGGRPRAVRFEEEM
>Mature_475_residues
AKIQQTFVCQSCGAVYNRWRGRCEACNGWNTIQEEVASAGPQSGPAATRPSRARGRVFPLEGLTGEAKEAPRTPSGINEL
DRVTGGGFVRGSVILLGGDPGIGKSTLLMQASAAMAKSGERVAYISGEEAVGQVRLRAERLGLAKYPVELAAQTNVEDIV
ETLSQGHPPALVIIDSIQTMWTETVESAPGTVTQVRSSAQALIRFAKTTGTAVILVGHVTKDGQIAGPRVVEHMVDAVAS
FEGDQGHHFRILRAVKNRFGPTDEIGVFEMTDAGLSEVPNPSALFLAGRDHAAPGTAVFAGMEGTRPLLVEIQALVAPSS
LGMPRRAVVGWDPNRLSMVLAVLEAHGGIRLGGHDVYLNVAGGLRITEPAADLAVAAALVSSLSGAALPSDSVYFGELGL
SGAVRPVSQAPARLKEALKLGFGKAITPQGRGEAGDRALPTDALRHIADLVAGIASGAPRKGGGRPRAVRFEEEM

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=438, Percent_Identity=48.1735159817352, Blast_Score=402, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR020588
- InterPro:   IPR004504
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC [H]

EC number: NA

Molecular weight: Translated: 49685; Mature: 49554

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIQQTFVCQSCGAVYNRWRGRCEACNGWNTIQEEVASAGPQSGPAATRPSRARGRVFP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
LEGLTGEAKEAPRTPSGINELDRVTGGGFVRGSVILLGGDPGIGKSTLLMQASAAMAKSG
CCCCCCCHHCCCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCC
ERVAYISGEEAVGQVRLRAERLGLAKYPVELAAQTNVEDIVETLSQGHPPALVIIDSIQT
CEEEEECCCHHHHHHHHHHHHCCCHHCCHHHHHCCCHHHHHHHHHCCCCCEEEEECCHHH
MWTETVESAPGTVTQVRSSAQALIRFAKTTGTAVILVGHVTKDGQIAGPRVVEHMVDAVA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHH
SFEGDQGHHFRILRAVKNRFGPTDEIGVFEMTDAGLSEVPNPSALFLAGRDHAAPGTAVF
HCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHCCCCCEEEEECCCCCCCCCEEE
AGMEGTRPLLVEIQALVAPSSLGMPRRAVVGWDPNRLSMVLAVLEAHGGIRLGGHDVYLN
ECCCCCCCHHEEHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEECCCEEEEE
VAGGLRITEPAADLAVAAALVSSLSGAALPSDSVYFGELGLSGAVRPVSQAPARLKEALK
ECCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
LGFGKAITPQGRGEAGDRALPTDALRHIADLVAGIASGAPRKGGGRPRAVRFEEEM
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCC
>Mature Secondary Structure 
AKIQQTFVCQSCGAVYNRWRGRCEACNGWNTIQEEVASAGPQSGPAATRPSRARGRVFP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
LEGLTGEAKEAPRTPSGINELDRVTGGGFVRGSVILLGGDPGIGKSTLLMQASAAMAKSG
CCCCCCCHHCCCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCC
ERVAYISGEEAVGQVRLRAERLGLAKYPVELAAQTNVEDIVETLSQGHPPALVIIDSIQT
CEEEEECCCHHHHHHHHHHHHCCCHHCCHHHHHCCCHHHHHHHHHCCCCCEEEEECCHHH
MWTETVESAPGTVTQVRSSAQALIRFAKTTGTAVILVGHVTKDGQIAGPRVVEHMVDAVA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHH
SFEGDQGHHFRILRAVKNRFGPTDEIGVFEMTDAGLSEVPNPSALFLAGRDHAAPGTAVF
HCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHCCCCCEEEEECCCCCCCCCEEE
AGMEGTRPLLVEIQALVAPSSLGMPRRAVVGWDPNRLSMVLAVLEAHGGIRLGGHDVYLN
ECCCCCCCHHEEHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEECCCEEEEE
VAGGLRITEPAADLAVAAALVSSLSGAALPSDSVYFGELGLSGAVRPVSQAPARLKEALK
ECCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
LGFGKAITPQGRGEAGDRALPTDALRHIADLVAGIASGAPRKGGGRPRAVRFEEEM
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]