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The map label for this gene is trmFO [H]

Identifier: 218532141

GI number: 218532141

Start: 4482078

End: 4483502

Strand: Reverse

Name: trmFO [H]

Synonym: Mchl_4253

Alternate gene names: 218532141

Gene position: 4483502-4482078 (Counterclockwise)

Preceding gene: 218532143

Following gene: 218532140

Centisome position: 77.6

GC content: 70.32

Gene sequence:

>1425_bases
ATGACGAACCCGATCCATATCGTCGGCGGTGGCCTCGCCGGTTCCGAAGCGGCCTGGCAGGTCGCGCAGGGCGGTCGCAC
GGTCGTGCTGCACGAGATGCGGCCCGTTCGCGCCACCGATGCCCACCACACCGACGGGCTGGCCGAACTGGTCTGCTCCA
ATTCCTTCCGCTCGGACGACGCGAACGGCAACGCCGTCGGCCTGCTGCATCAGGAGATGCGCAGCCTCGACTCGCTGATC
ATGCGCACGGCCGACGCCCACCAAGTGCCCGCGGGCGGGGCGCTCGCCGTCGATCGCGAAGGGTTTTCGCGGGCCGTCAC
CGCGGCGCTGGAGGATCACCCCAACATCACCATCGTCCGTGAGGAGGTCGCCGGCCTTCCCCCCGAGGACTGGGGTTCGA
CCATCCTCGCCACCGGCCCCCTCACCTCCCCGGCGCTGGCCGAGGCCGTCGGCGCGCTGACGGGCCGGGAATCGCTCGCC
TTCTTCGACGCCATCGCCCCGATCGTCCACCGCGACTCGATCGACATGGGCAAGGCGTGGTTCCAGTCCCGCTACGACAA
GGCCGGCCCCGGCGGCACGGGGGCGGATTACCTCAACTGCCCGATGGACCGGGAGCAGTACGAGGCCTTCGTCGCCGCGT
TGATCGCGGGCGAGAAGACGGCGTTCAAGGAATGGGAGGCCTCGACCCCCTATTTCGACGGCTGCCTGCCGATCGAGGTG
ATGGCCGAGCGTGGGCCCGAGACCCTGCGCCACGGGCCGATGAAGCCGGTTGGGCTCACCAATCCGCACAACCCGACGGT
CAAGGCCTATGCCATCGTCCAACTGCGTCAGGACAATGCGCTCGGCACCCTGTTCAACATGGTGGGCTTCCAGACCAAGC
TGCGCCACGCCGAGCAGGTCCGGGTCTTCCGCACGATTCCGGGGCTGGAGAATGCCGAGTTCGCCCGGCTCGGCGGCCTG
CACCGCAACACCTATCTCGACAGCCCGCGCCTGCTCGACGCGACCCTGCGGCTGAAGGCACGGCCCCAGCTTCGCTTCGC
CGGCCAGATCACCGGATGCGAGGGGTACGTCGAGAGCGCCGCGGTCGGGCTGATGGCCGGCCGCTACGCGCTCGCCGAGG
CGGAGGGGCAGACGCTCGCTCCCCTGCCCCCGACGACCGCGCTCGGGGCGCTCATCGGCCACATCACCGGCGGCCATGTC
GAGGCGCCCGAGGAGGCGGAGCGCAACGCACCGCGCTCGTTCCAGCCGATGAACGTGAATTTCGGGCTGTTCCCGCCGCT
GGCGCAGATGCCCCGCAACGAGACCGGCAAGCGCCTGCGCGGGCCCGAGAAGGCGGCGCTCAAGAAGCGCGCGCTCACCG
ACCGGGCGCGGGAAGACCTCGCTGCCTGGATGACGGGTGAGCGACTTCCGCACGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
GCGATGGAGCGCATCCTGTCCTTCGCGCCGGCGGCGCCCCGCCTCGCCTGCGGTGCCCGCCCGCATTGATCACGCGGCCG
CGGGGTTCTATTCCGTTGCG

Downstream 100 bases:

>100_bases
AGAGCGGCTCCGCCGGCTTCACGGCGCGGCCCTGAACGCCCGCCAAAGGGCCGTGAGGCCCTTTGGGAACCCGGTGACCT
CAGAACGGACCGGCACGGCA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTNPIHIVGGGLAGSEAAWQVAQGGRTVVLHEMRPVRATDAHHTDGLAELVCSNSFRSDDANGNAVGLLHQEMRSLDSLI
MRTADAHQVPAGGALAVDREGFSRAVTAALEDHPNITIVREEVAGLPPEDWGSTILATGPLTSPALAEAVGALTGRESLA
FFDAIAPIVHRDSIDMGKAWFQSRYDKAGPGGTGADYLNCPMDREQYEAFVAALIAGEKTAFKEWEASTPYFDGCLPIEV
MAERGPETLRHGPMKPVGLTNPHNPTVKAYAIVQLRQDNALGTLFNMVGFQTKLRHAEQVRVFRTIPGLENAEFARLGGL
HRNTYLDSPRLLDATLRLKARPQLRFAGQITGCEGYVESAAVGLMAGRYALAEAEGQTLAPLPPTTALGALIGHITGGHV
EAPEEAERNAPRSFQPMNVNFGLFPPLAQMPRNETGKRLRGPEKAALKKRALTDRAREDLAAWMTGERLPHAAE

Sequences:

>Translated_474_residues
MTNPIHIVGGGLAGSEAAWQVAQGGRTVVLHEMRPVRATDAHHTDGLAELVCSNSFRSDDANGNAVGLLHQEMRSLDSLI
MRTADAHQVPAGGALAVDREGFSRAVTAALEDHPNITIVREEVAGLPPEDWGSTILATGPLTSPALAEAVGALTGRESLA
FFDAIAPIVHRDSIDMGKAWFQSRYDKAGPGGTGADYLNCPMDREQYEAFVAALIAGEKTAFKEWEASTPYFDGCLPIEV
MAERGPETLRHGPMKPVGLTNPHNPTVKAYAIVQLRQDNALGTLFNMVGFQTKLRHAEQVRVFRTIPGLENAEFARLGGL
HRNTYLDSPRLLDATLRLKARPQLRFAGQITGCEGYVESAAVGLMAGRYALAEAEGQTLAPLPPTTALGALIGHITGGHV
EAPEEAERNAPRSFQPMNVNFGLFPPLAQMPRNETGKRLRGPEKAALKKRALTDRAREDLAAWMTGERLPHAAE
>Mature_473_residues
TNPIHIVGGGLAGSEAAWQVAQGGRTVVLHEMRPVRATDAHHTDGLAELVCSNSFRSDDANGNAVGLLHQEMRSLDSLIM
RTADAHQVPAGGALAVDREGFSRAVTAALEDHPNITIVREEVAGLPPEDWGSTILATGPLTSPALAEAVGALTGRESLAF
FDAIAPIVHRDSIDMGKAWFQSRYDKAGPGGTGADYLNCPMDREQYEAFVAALIAGEKTAFKEWEASTPYFDGCLPIEVM
AERGPETLRHGPMKPVGLTNPHNPTVKAYAIVQLRQDNALGTLFNMVGFQTKLRHAEQVRVFRTIPGLENAEFARLGGLH
RNTYLDSPRLLDATLRLKARPQLRFAGQITGCEGYVESAAVGLMAGRYALAEAEGQTLAPLPPTTALGALIGHITGGHVE
APEEAERNAPRSFQPMNVNFGLFPPLAQMPRNETGKRLRGPEKAALKKRALTDRAREDLAAWMTGERLPHAAE

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 51029; Mature: 50898

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPIHIVGGGLAGSEAAWQVAQGGRTVVLHEMRPVRATDAHHTDGLAELVCSNSFRSDD
CCCCEEEEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCC
ANGNAVGLLHQEMRSLDSLIMRTADAHQVPAGGALAVDREGFSRAVTAALEDHPNITIVR
CCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEE
EEVAGLPPEDWGSTILATGPLTSPALAEAVGALTGRESLAFFDAIAPIVHRDSIDMGKAW
HHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHH
FQSRYDKAGPGGTGADYLNCPMDREQYEAFVAALIAGEKTAFKEWEASTPYFDGCLPIEV
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHH
MAERGPETLRHGPMKPVGLTNPHNPTVKAYAIVQLRQDNALGTLFNMVGFQTKLRHAEQV
HHHCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
RVFRTIPGLENAEFARLGGLHRNTYLDSPRLLDATLRLKARPQLRFAGQITGCEGYVESA
HHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCEEHEEEEECCCCEEEEEEECCCHHHHHHH
AVGLMAGRYALAEAEGQTLAPLPPTTALGALIGHITGGHVEAPEEAERNAPRSFQPMNVN
HHHHHHHHHHEECCCCCEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCC
FGLFPPLAQMPRNETGKRLRGPEKAALKKRALTDRAREDLAAWMTGERLPHAAE
CCCCCCHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TNPIHIVGGGLAGSEAAWQVAQGGRTVVLHEMRPVRATDAHHTDGLAELVCSNSFRSDD
CCCEEEEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCC
ANGNAVGLLHQEMRSLDSLIMRTADAHQVPAGGALAVDREGFSRAVTAALEDHPNITIVR
CCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEE
EEVAGLPPEDWGSTILATGPLTSPALAEAVGALTGRESLAFFDAIAPIVHRDSIDMGKAW
HHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHH
FQSRYDKAGPGGTGADYLNCPMDREQYEAFVAALIAGEKTAFKEWEASTPYFDGCLPIEV
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHH
MAERGPETLRHGPMKPVGLTNPHNPTVKAYAIVQLRQDNALGTLFNMVGFQTKLRHAEQV
HHHCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
RVFRTIPGLENAEFARLGGLHRNTYLDSPRLLDATLRLKARPQLRFAGQITGCEGYVESA
HHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCEEHEEEEECCCCEEEEEEECCCHHHHHHH
AVGLMAGRYALAEAEGQTLAPLPPTTALGALIGHITGGHVEAPEEAERNAPRSFQPMNVN
HHHHHHHHHHEECCCCCEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCC
FGLFPPLAQMPRNETGKRLRGPEKAALKKRALTDRAREDLAAWMTGERLPHAAE
CCCCCCHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA