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The map label for this gene is wcaJ [H]

Identifier: 218532133

GI number: 218532133

Start: 4464710

End: 4465372

Strand: Reverse

Name: wcaJ [H]

Synonym: Mchl_4239

Alternate gene names: 218532133

Gene position: 4465372-4464710 (Counterclockwise)

Preceding gene: 218532134

Following gene: 218532130

Centisome position: 77.28

GC content: 63.5

Gene sequence:

>663_bases
ATGAATATGAGAGGTCGCGACCTGGCGCTGCCCGAACAGCAGGAACCGTTGAACAGCAGTTCACGGGATTTGCGCCCGGC
TCTGCGCACCAGCGTCGACGTTGCGCTGGCTGTGGTCGCACTGTTCCTGTTGGCGCCGCTGCTGGCGCTGATTGCCGTGC
TGGTCAAGCTTGATAGCCGAGGTGCTGCCCTGTTCCGGCAGACCCGCACCGGCCGGAACGGACACACCTTTCTGATCTAC
AAGTTCCGCACCATGCACGTGCAGGAGAACGGCGCAGAGGTGCATCAAGCCTGCCGTGGCGATAGGCGCGTGACCCGGCT
CGGCGCCTTCCTGCGTCAGACCAGCCTGGATGAGCTGCCGCAGCTATTGAACGTCCTGCGCGGTGAAATGGCGCTGGTTG
GTCCGCGTCCCCACGCCATGGCGCACGACACCTACTACGGTGCCCAGATCACAGCTTACGCACGTCGCTTTGCCGTGCGC
CCCGGCATTACCGGCTGGGCGCAGATCAACGGCTCGCGCGGAGCTACCCCAACCCTAGAAGACATGCAGCGTCGGGTCGA
GCTTGATCTCTGGTATGTTCGAAACCGTAGCTTCGCGCTGGATCTGCACATCCTCGCCGCAACCGCTCTGGTGGCCTTGG
TTCGGCGCACCGAAGCCTACTGA

Upstream 100 bases:

>100_bases
AATGTAAACACTTCCCGCGACCGTCCCATAAAGAGCCATTCGGGCCAGGGGCATGTCGCAGACAACCTTAGTTGCGTTCT
TACAAGCGTAGGGGGCACCA

Downstream 100 bases:

>100_bases
GCGGTCACAGCTGCGGGTCGCTCGTGGGATTCTCAGATTGGCGGCCTGCTTGGTCGGAAGCTCCGTCCCCCATAACTGCA
TCGTGGCTCGTCTCGTCTGC

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNMRGRDLALPEQQEPLNSSSRDLRPALRTSVDVALAVVALFLLAPLLALIAVLVKLDSRGAALFRQTRTGRNGHTFLIY
KFRTMHVQENGAEVHQACRGDRRVTRLGAFLRQTSLDELPQLLNVLRGEMALVGPRPHAMAHDTYYGAQITAYARRFAVR
PGITGWAQINGSRGATPTLEDMQRRVELDLWYVRNRSFALDLHILAATALVALVRRTEAY

Sequences:

>Translated_220_residues
MNMRGRDLALPEQQEPLNSSSRDLRPALRTSVDVALAVVALFLLAPLLALIAVLVKLDSRGAALFRQTRTGRNGHTFLIY
KFRTMHVQENGAEVHQACRGDRRVTRLGAFLRQTSLDELPQLLNVLRGEMALVGPRPHAMAHDTYYGAQITAYARRFAVR
PGITGWAQINGSRGATPTLEDMQRRVELDLWYVRNRSFALDLHILAATALVALVRRTEAY
>Mature_220_residues
MNMRGRDLALPEQQEPLNSSSRDLRPALRTSVDVALAVVALFLLAPLLALIAVLVKLDSRGAALFRQTRTGRNGHTFLIY
KFRTMHVQENGAEVHQACRGDRRVTRLGAFLRQTSLDELPQLLNVLRGEMALVGPRPHAMAHDTYYGAQITAYARRFAVR
PGITGWAQINGSRGATPTLEDMQRRVELDLWYVRNRSFALDLHILAATALVALVRRTEAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=208, Percent_Identity=49.0384615384615, Blast_Score=201, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 24641; Mature: 24641

Theoretical pI: Translated: 11.14; Mature: 11.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMRGRDLALPEQQEPLNSSSRDLRPALRTSVDVALAVVALFLLAPLLALIAVLVKLDSR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GAALFRQTRTGRNGHTFLIYKFRTMHVQENGAEVHQACRGDRRVTRLGAFLRQTSLDELP
CHHHHHHHCCCCCCCEEEEEEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHH
QLLNVLRGEMALVGPRPHAMAHDTYYGAQITAYARRFAVRPGITGWAQINGSRGATPTLE
HHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHH
DMQRRVELDLWYVRNRSFALDLHILAATALVALVRRTEAY
HHHHHHHHHEEEEECCCEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNMRGRDLALPEQQEPLNSSSRDLRPALRTSVDVALAVVALFLLAPLLALIAVLVKLDSR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GAALFRQTRTGRNGHTFLIYKFRTMHVQENGAEVHQACRGDRRVTRLGAFLRQTSLDELP
CHHHHHHHCCCCCCCEEEEEEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHH
QLLNVLRGEMALVGPRPHAMAHDTYYGAQITAYARRFAVRPGITGWAQINGSRGATPTLE
HHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHH
DMQRRVELDLWYVRNRSFALDLHILAATALVALVRRTEAY
HHHHHHHHHEEEEECCCEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]