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The map label for this gene is cisA [H]

Identifier: 218532099

GI number: 218532099

Start: 4418669

End: 4420336

Strand: Reverse

Name: cisA [H]

Synonym: Mchl_4193

Alternate gene names: 218532099

Gene position: 4420336-4418669 (Counterclockwise)

Preceding gene: 218532111

Following gene: 218532098

Centisome position: 76.5

GC content: 67.87

Gene sequence:

>1668_bases
ATGAACCATCAGACCCGCAAGGCGCCCTTCGTGCCGGTGAGCGACCGGCCGCAGCGCGTCGGCTGCTACCTGCGCGTGTC
CACCGGTCGGCAGGCGGAGGGCGACCTCTCGATCCCTGACCAGCGCAAGCAGGTCCACGCCTTCTGCGCCGCCAAGGGCT
GGCCGGTCGTCGGCGAGTACGTCGAGCCGGGTGCCTCGGCCACCGATGACAACCGCCCCGAGTTCCAGAAGATGATCGAG
CGCGCGGGTGACGACGACCACCCCTTCGACGTCATCGTCGTGCACTCCTACTCGCGCTTCTTCCGCGACAGCTTCGGCCT
GGAGATGTACGTGCGCCGGCTGGCCAGGGCCGGCGTTCGGCTTATCTCGATTACCCAGGAACTCGGCGATGACCCCGCCC
AGGTGATGATGCGCCAGGTCATCGCCCTGTTCGACGAGTACCAGTCGAAGGAGAATGCCAAGCACGTGCTGCGGGCGATG
AAGGAGAACGCGCGGCAAGGCTTCTACAACGGTTCGCCGGTGCCGCTCGGCTACACGGTCGCGGAGATCGAGAAGCGCGG
CGCCAGGGTCAAGAAGCGCCTCGCCGTCGATCCGGTCGAGGCCGAGACGGTCAGGCTGATCTTCCGGCTGTACCGGTTCG
GCGACGGCGCCACCGGTCCGCTGGGCGTCAAGGCGATCACCTGCCACCTGAACGAGCGCGGCTATCGCACCCGGGCTGGG
GCGCGGTTCGGGGTCGCCACGGTACACGGCATCCTGACCAACCGGGTCTATGTCGGCGAGGCTTTTTTCAACCGGCGCTG
CTCGAAGACGCTGGCCCAGAAGCCTGCAGCCGAACACATCCCGATCAGCGTGCCGGCGCTCATCGAGCTGCCCGAGTTCG
AAGCCGTGCAGGCGACTTTGAAGGCGCACAACCCGCGTGCGACGCCGGCCCGCGTGGTGACCGGCCCGATCCTGCTGACG
GGTCTGGCCACCTGCACGACCTGCAACGGACCGATGACGCTGCGCACCGGCACGTCGAAGTCGGGGAAGGTCCATCGCTA
CTACAGCTGCTCGACGCATGGCCGGATGGGCAGGACCGGCTGCACCGGCCGCAGCATTCCGATGGACACGCTCGACACGC
TGGTGACCGACCATCTTGTCGCCGAACTGCTGCAACCGGACCGGCTGCGGGCGACGCTGGCCTCGCTGTGGGCGCTGCGG
GCCGAGAAGGCGGCGCAGGTCGACGGCCGCGTGGCGGCTTTGCGCGGCGAGGTGTGCGAAGCGGAGGAGAAGCTGAAGCG
GCTCTACCGGATGGTCGAGGAGGGGGTGACCGATCTCGACGACATCTTGAAGGGGCGTTTGGCGGCGCTGAAGGAGGACC
GGGACCGGGCCCGGACCGCCCTTGACCGGATCCACGTGGCCGAGCGTGCGCCGGCCGAGATCGCGCCTGAGCTCGTCGAG
AAGTTCGGGCGTCTGATGCGCGAGAACGTGACGAGCGGCGAGATCCCGTTCCGGAAGGCGTGGCTGCAGGCGATCGTCGA
CCGAGTCGAGGTGGACGCAGACGTCATCCGCATCGTCGGCGAAAAGGCGAGCCTTGAGGCCGCCGTCATCGATGCGGCCC
CATCAGCCGCGCCAGGTGTTCGCAGTTCTGTACGGAAGTGGCGCGCCCGAAAGGATTCGAACCTCTGA

Upstream 100 bases:

>100_bases
TACCTGTGGAGTGAGCTGGGCCGACTCTTGCTGCTGAGTCGCGCCCTCCTCAGCCTCCCTGTTCGAATAAATCTGTTCCC
AAAGCGTCAAGGCCCGCGCA

Downstream 100 bases:

>100_bases
CCCCCAGATTCGTAGTCTGGTGCTCTATCCAGCTGAGCTACGGGCGCCTTTTTTACTTCTTGACGCCCTTGCGGGGCGTT
CCTCTTGTTGCTTGGTCGGA

Product: Resolvase domain

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MNHQTRKAPFVPVSDRPQRVGCYLRVSTGRQAEGDLSIPDQRKQVHAFCAAKGWPVVGEYVEPGASATDDNRPEFQKMIE
RAGDDDHPFDVIVVHSYSRFFRDSFGLEMYVRRLARAGVRLISITQELGDDPAQVMMRQVIALFDEYQSKENAKHVLRAM
KENARQGFYNGSPVPLGYTVAEIEKRGARVKKRLAVDPVEAETVRLIFRLYRFGDGATGPLGVKAITCHLNERGYRTRAG
ARFGVATVHGILTNRVYVGEAFFNRRCSKTLAQKPAAEHIPISVPALIELPEFEAVQATLKAHNPRATPARVVTGPILLT
GLATCTTCNGPMTLRTGTSKSGKVHRYYSCSTHGRMGRTGCTGRSIPMDTLDTLVTDHLVAELLQPDRLRATLASLWALR
AEKAAQVDGRVAALRGEVCEAEEKLKRLYRMVEEGVTDLDDILKGRLAALKEDRDRARTALDRIHVAERAPAEIAPELVE
KFGRLMRENVTSGEIPFRKAWLQAIVDRVEVDADVIRIVGEKASLEAAVIDAAPSAAPGVRSSVRKWRARKDSNL

Sequences:

>Translated_555_residues
MNHQTRKAPFVPVSDRPQRVGCYLRVSTGRQAEGDLSIPDQRKQVHAFCAAKGWPVVGEYVEPGASATDDNRPEFQKMIE
RAGDDDHPFDVIVVHSYSRFFRDSFGLEMYVRRLARAGVRLISITQELGDDPAQVMMRQVIALFDEYQSKENAKHVLRAM
KENARQGFYNGSPVPLGYTVAEIEKRGARVKKRLAVDPVEAETVRLIFRLYRFGDGATGPLGVKAITCHLNERGYRTRAG
ARFGVATVHGILTNRVYVGEAFFNRRCSKTLAQKPAAEHIPISVPALIELPEFEAVQATLKAHNPRATPARVVTGPILLT
GLATCTTCNGPMTLRTGTSKSGKVHRYYSCSTHGRMGRTGCTGRSIPMDTLDTLVTDHLVAELLQPDRLRATLASLWALR
AEKAAQVDGRVAALRGEVCEAEEKLKRLYRMVEEGVTDLDDILKGRLAALKEDRDRARTALDRIHVAERAPAEIAPELVE
KFGRLMRENVTSGEIPFRKAWLQAIVDRVEVDADVIRIVGEKASLEAAVIDAAPSAAPGVRSSVRKWRARKDSNL
>Mature_555_residues
MNHQTRKAPFVPVSDRPQRVGCYLRVSTGRQAEGDLSIPDQRKQVHAFCAAKGWPVVGEYVEPGASATDDNRPEFQKMIE
RAGDDDHPFDVIVVHSYSRFFRDSFGLEMYVRRLARAGVRLISITQELGDDPAQVMMRQVIALFDEYQSKENAKHVLRAM
KENARQGFYNGSPVPLGYTVAEIEKRGARVKKRLAVDPVEAETVRLIFRLYRFGDGATGPLGVKAITCHLNERGYRTRAG
ARFGVATVHGILTNRVYVGEAFFNRRCSKTLAQKPAAEHIPISVPALIELPEFEAVQATLKAHNPRATPARVVTGPILLT
GLATCTTCNGPMTLRTGTSKSGKVHRYYSCSTHGRMGRTGCTGRSIPMDTLDTLVTDHLVAELLQPDRLRATLASLWALR
AEKAAQVDGRVAALRGEVCEAEEKLKRLYRMVEEGVTDLDDILKGRLAALKEDRDRARTALDRIHVAERAPAEIAPELVE
KFGRLMRENVTSGEIPFRKAWLQAIVDRVEVDADVIRIVGEKASLEAAVIDAAPSAAPGVRSSVRKWRARKDSNL

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 61509; Mature: 61509

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHQTRKAPFVPVSDRPQRVGCYLRVSTGRQAEGDLSIPDQRKQVHAFCAAKGWPVVGEY
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHH
VEPGASATDDNRPEFQKMIERAGDDDHPFDVIVVHSYSRFFRDSFGLEMYVRRLARAGVR
CCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHCCHH
LISITQELGDDPAQVMMRQVIALFDEYQSKENAKHVLRAMKENARQGFYNGSPVPLGYTV
HEEEHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
AEIEKRGARVKKRLAVDPVEAETVRLIFRLYRFGDGATGPLGVKAITCHLNERGYRTRAG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCC
ARFGVATVHGILTNRVYVGEAFFNRRCSKTLAQKPAAEHIPISVPALIELPEFEAVQATL
CCHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHEECCCHHHHHHHH
KAHNPRATPARVVTGPILLTGLATCTTCNGPMTLRTGTSKSGKVHRYYSCSTHGRMGRTG
HHCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCEEEEEECCCCCCCCCCC
CTGRSIPMDTLDTLVTDHLVAELLQPDRLRATLASLWALRAEKAAQVDGRVAALRGEVCE
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AEEKLKRLYRMVEEGVTDLDDILKGRLAALKEDRDRARTALDRIHVAERAPAEIAPELVE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KFGRLMRENVTSGEIPFRKAWLQAIVDRVEVDADVIRIVGEKASLEAAVIDAAPSAAPGV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCCCCH
RSSVRKWRARKDSNL
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MNHQTRKAPFVPVSDRPQRVGCYLRVSTGRQAEGDLSIPDQRKQVHAFCAAKGWPVVGEY
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHH
VEPGASATDDNRPEFQKMIERAGDDDHPFDVIVVHSYSRFFRDSFGLEMYVRRLARAGVR
CCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHCCHH
LISITQELGDDPAQVMMRQVIALFDEYQSKENAKHVLRAMKENARQGFYNGSPVPLGYTV
HEEEHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
AEIEKRGARVKKRLAVDPVEAETVRLIFRLYRFGDGATGPLGVKAITCHLNERGYRTRAG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCC
ARFGVATVHGILTNRVYVGEAFFNRRCSKTLAQKPAAEHIPISVPALIELPEFEAVQATL
CCHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCHHEECCCHHHHHHHH
KAHNPRATPARVVTGPILLTGLATCTTCNGPMTLRTGTSKSGKVHRYYSCSTHGRMGRTG
HHCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCEEEEEECCCCCCCCCCC
CTGRSIPMDTLDTLVTDHLVAELLQPDRLRATLASLWALRAEKAAQVDGRVAALRGEVCE
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AEEKLKRLYRMVEEGVTDLDDILKGRLAALKEDRDRARTALDRIHVAERAPAEIAPELVE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KFGRLMRENVTSGEIPFRKAWLQAIVDRVEVDADVIRIVGEKASLEAAVIDAAPSAAPGV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCCCCH
RSSVRKWRARKDSNL
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]