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The map label for this gene is bepF [H]

Identifier: 218532046

GI number: 218532046

Start: 4366322

End: 4367632

Strand: Reverse

Name: bepF [H]

Synonym: Mchl_4140

Alternate gene names: 218532046

Gene position: 4367632-4366322 (Counterclockwise)

Preceding gene: 218532047

Following gene: 218532045

Centisome position: 75.59

GC content: 68.73

Gene sequence:

>1311_bases
ATGACGATGACCGGCATGTTCACGCTCCGGGTCTGGGTGGCCGCCGCAACGCTGTCCGCCGCGTGCCCGCCCGTCATTTC
GACGGCGTTCGCGCAGGCCGAGCCGACGGGCCCCCCCGTCGTCGGCATCATCGCCGCGCGCAAGGAGCCGATCACCCAGA
CGGAGCAGTTCATCGGCCGCATCGAGGCGAAGCAGCGTGTCGACATCGTCGCCCGGGTGACGGCGTTCATCGAGAAGCGG
CTCTTCGTCGAGGGCACGGATGTCGAGGAAGGCGCGCTCCTCTACCTTCTCGAGCAGGGCCCGTTCCAGGCGGTCGTGGC
CGCGCAGAAGGCGCAGATTGCGCAGGTCGAAGCGCAATTGGAGAACGCGCGTCTCACGACGCAGCGCGCCCGAACCCTGC
TCGGCGGGCCGGCCGGGCAGCAATCGACCTACGACGCGGCCCTCGCGAACGAGCGCGCCTTGGAGGCGCAGCTCCAAGGC
GCACAAGCTCAGCTCGACACGGCGCAGATCAATCTCGGCTACACGGAGATCCGCGCGCCGATTGCGGGCCAGATCGGGCG
CACGTCCCTGACGATCGGCAACGTCGTCGGGCCGGGCTCCGGGGTGCTGACGACGATCATCAGCCAGGACCCGATGTTCG
TGACGTTTCCGGTGCCGGTCCGTGAAGCGATCAATCTGAGAGCGCGCTATGGGCCGACGGGCGGATTCGGCGCCGTCCTG
ATCCGGGCACAACTCCCGGACGGCCGCTTCTACGATCACACCGGGACGCTCGATTTCGTCAACAACACCATCGCGACGAA
TACGGACACGATCCTGCTTCGCGGCGTGCTGCCGAACCCTCCGATCTTCGGTCAAACCCGCAGCTTGCTCACCCTTCGTG
AACTCACGGATGGCGAGTTCGTGACCGTGTTCCTCGAGGGCGTCGAGCCGGTCGAGGTGGTCGCGATTCCCCGCGCCGCC
GTCCTGGCCGACCAGCGGGGCAGCTACGTCTACGTGATCGGGCCGGGGAATAAGGCCGAGGAGCGTCGGATTCAGCTCGG
CCAGTCGAGCACGACGCTCGCGGCGGTGACGAGCGGTCTCAAGGTCGGAGAGCAGGTCATCGCCGAAGGCTTGCAGCGGG
TCCGCGCGGGCGAGACGGTCTCGCCCAAACCCGCCGACGCCATTCTGCAGAGGATGATGCGGAATTCGGCGGTGAACGCG
GACCGCGCAGGGCCGGCTGCGTCCGCTTCGCCGCCGGCCGGACAAGCTCCGACCGGAGCCGCCAGCGGCGCCGGTCCGAG
CGCGAGCGGCAGCCCGAACAGCCGGCGCTAG

Upstream 100 bases:

>100_bases
ATGCAAATGCATCGGCAATAAACCGTCGTTCTAGAGCGCATTCCGACGAAGTGCTCACCGGTTCGGCGAAAGAATGCGTG
TCAAACCCCCGGAGGCGAAC

Downstream 100 bases:

>100_bases
CGCAGCATGGGACGCCGCCTCCCGTTCCGCCCCCCTTGTCCCGCTCCGCGGCCCCTCGTCCCGGATCGCACTTCGTTTGT
CCCAGGCGGCCGAGCCCGAG

Product: efflux transporter RND family, MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTMTGMFTLRVWVAAATLSAACPPVISTAFAQAEPTGPPVVGIIAARKEPITQTEQFIGRIEAKQRVDIVARVTAFIEKR
LFVEGTDVEEGALLYLLEQGPFQAVVAAQKAQIAQVEAQLENARLTTQRARTLLGGPAGQQSTYDAALANERALEAQLQG
AQAQLDTAQINLGYTEIRAPIAGQIGRTSLTIGNVVGPGSGVLTTIISQDPMFVTFPVPVREAINLRARYGPTGGFGAVL
IRAQLPDGRFYDHTGTLDFVNNTIATNTDTILLRGVLPNPPIFGQTRSLLTLRELTDGEFVTVFLEGVEPVEVVAIPRAA
VLADQRGSYVYVIGPGNKAEERRIQLGQSSTTLAAVTSGLKVGEQVIAEGLQRVRAGETVSPKPADAILQRMMRNSAVNA
DRAGPAASASPPAGQAPTGAASGAGPSASGSPNSRR

Sequences:

>Translated_436_residues
MTMTGMFTLRVWVAAATLSAACPPVISTAFAQAEPTGPPVVGIIAARKEPITQTEQFIGRIEAKQRVDIVARVTAFIEKR
LFVEGTDVEEGALLYLLEQGPFQAVVAAQKAQIAQVEAQLENARLTTQRARTLLGGPAGQQSTYDAALANERALEAQLQG
AQAQLDTAQINLGYTEIRAPIAGQIGRTSLTIGNVVGPGSGVLTTIISQDPMFVTFPVPVREAINLRARYGPTGGFGAVL
IRAQLPDGRFYDHTGTLDFVNNTIATNTDTILLRGVLPNPPIFGQTRSLLTLRELTDGEFVTVFLEGVEPVEVVAIPRAA
VLADQRGSYVYVIGPGNKAEERRIQLGQSSTTLAAVTSGLKVGEQVIAEGLQRVRAGETVSPKPADAILQRMMRNSAVNA
DRAGPAASASPPAGQAPTGAASGAGPSASGSPNSRR
>Mature_435_residues
TMTGMFTLRVWVAAATLSAACPPVISTAFAQAEPTGPPVVGIIAARKEPITQTEQFIGRIEAKQRVDIVARVTAFIEKRL
FVEGTDVEEGALLYLLEQGPFQAVVAAQKAQIAQVEAQLENARLTTQRARTLLGGPAGQQSTYDAALANERALEAQLQGA
QAQLDTAQINLGYTEIRAPIAGQIGRTSLTIGNVVGPGSGVLTTIISQDPMFVTFPVPVREAINLRARYGPTGGFGAVLI
RAQLPDGRFYDHTGTLDFVNNTIATNTDTILLRGVLPNPPIFGQTRSLLTLRELTDGEFVTVFLEGVEPVEVVAIPRAAV
LADQRGSYVYVIGPGNKAEERRIQLGQSSTTLAAVTSGLKVGEQVIAEGLQRVRAGETVSPKPADAILQRMMRNSAVNAD
RAGPAASASPPAGQAPTGAASGAGPSASGSPNSRR

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=383, Percent_Identity=31.5926892950392, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789665, Length=359, Percent_Identity=30.9192200557103, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1789929, Length=345, Percent_Identity=27.8260869565217, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87082036, Length=335, Percent_Identity=24.1791044776119, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081796, Length=331, Percent_Identity=28.3987915407855, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45812; Mature: 45681

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTGMFTLRVWVAAATLSAACPPVISTAFAQAEPTGPPVVGIIAARKEPITQTEQFIGR
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHH
IEAKQRVDIVARVTAFIEKRLFVEGTDVEEGALLYLLEQGPFQAVVAAQKAQIAQVEAQL
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ENARLTTQRARTLLGGPAGQQSTYDAALANERALEAQLQGAQAQLDTAQINLGYTEIRAP
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHCCCHHEECEEEEECCHHHHCCC
IAGQIGRTSLTIGNVVGPGSGVLTTIISQDPMFVTFPVPVREAINLRARYGPTGGFGAVL
CCCCCCCCEEEECCCCCCCCCCEEHEECCCCEEEEECCCHHHHHCCCEECCCCCCCEEEE
IRAQLPDGRFYDHTGTLDFVNNTIATNTDTILLRGVLPNPPIFGQTRSLLTLRELTDGEF
EEEECCCCCEECCCCCHHHHCCEECCCCCEEEEEECCCCCCCCCCCHHHEEEECCCCCCE
VTVFLEGVEPVEVVAIPRAAVLADQRGSYVYVIGPGNKAEERRIQLGQSSTTLAAVTSGL
EEEEECCCCCEEEEEECCHHEEECCCCCEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHH
KVGEQVIAEGLQRVRAGETVSPKPADAILQRMMRNSAVNADRAGPAASASPPAGQAPTGA
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
ASGAGPSASGSPNSRR
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMTGMFTLRVWVAAATLSAACPPVISTAFAQAEPTGPPVVGIIAARKEPITQTEQFIGR
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHH
IEAKQRVDIVARVTAFIEKRLFVEGTDVEEGALLYLLEQGPFQAVVAAQKAQIAQVEAQL
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ENARLTTQRARTLLGGPAGQQSTYDAALANERALEAQLQGAQAQLDTAQINLGYTEIRAP
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHCCCHHEECEEEEECCHHHHCCC
IAGQIGRTSLTIGNVVGPGSGVLTTIISQDPMFVTFPVPVREAINLRARYGPTGGFGAVL
CCCCCCCCEEEECCCCCCCCCCEEHEECCCCEEEEECCCHHHHHCCCEECCCCCCCEEEE
IRAQLPDGRFYDHTGTLDFVNNTIATNTDTILLRGVLPNPPIFGQTRSLLTLRELTDGEF
EEEECCCCCEECCCCCHHHHCCEECCCCCEEEEEECCCCCCCCCCCHHHEEEECCCCCCE
VTVFLEGVEPVEVVAIPRAAVLADQRGSYVYVIGPGNKAEERRIQLGQSSTTLAAVTSGL
EEEEECCCCCEEEEEECCHHEEECCCCCEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHH
KVGEQVIAEGLQRVRAGETVSPKPADAILQRMMRNSAVNADRAGPAASASPPAGQAPTGA
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
ASGAGPSASGSPNSRR
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]