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The map label for this gene is glnT [H]

Identifier: 218531933

GI number: 218531933

Start: 4230524

End: 4231897

Strand: Reverse

Name: glnT [H]

Synonym: Mchl_4021

Alternate gene names: 218531933

Gene position: 4231897-4230524 (Counterclockwise)

Preceding gene: 218531934

Following gene: 218531932

Centisome position: 73.24

GC content: 67.69

Gene sequence:

>1374_bases
ATGGGCCACGCGAACGACATCGATCCGCAGGTGCGTGCCGTGCAGGAGGATCTGCGCGCCAAGGGCGTGAAATACGTCAT
CGGCGCCTATGTCGACATCCACGGCGCGCAGAAGGCCAAGGTGGTGCCGATCGACCACCTGCCGCAGATGGCGGCGGGCT
CCGAGCGCTATACCGGCTACGCCCTCGACGGCCTGGGCCAGGCGCCGAACGAGGACGAACTCGCCTCGGTCCCGGATTTC
TCGCAGGTCATCCAGCTCCCCTGGGAGAGCAAGCTCGCCTGGGCGCCGGCCGACCTCACCTTCCAGGGCAAGCCCTACCC
CCTCTCGACCCGCGTCGCCCTCAAGAACGTCCTGGCTCAGGCCGAGGCGATGGGCTTCGGCTTCAATCTCGGCATCGAGT
GCGAGATCTTCCTGCTGCGCCAAGCCGCCGACGGCTCGCTGCACACGCCGGTGGCGGACGATAAGCTGGTGAAGCCCTGC
TACGACGTGCGCGGCTTCATCGACAACTTCTCCTGGCTCGACAAGGTCGCGACCACGATCAACGATCTCGGCTGGGATCT
GTACTCGTTCGACCATGAGGATGCGAACGCGCAGTTCGAGTTCGACTTCCAGTACGCTGATGCGCTGACCATGTGCGATC
GGCTGACCTTCTTCCGCATGATGACCAAGCATTACGCGAAGGAGGAAGGGCTCATCGCCACGATGATGCCCAAGCCCTTC
GCCGACCGCACCGGCAACGGTGCCCACTTCAACATGTCGCTCTCCGACCGCGAGACCGGCCGCAATCTCTTCGCCTGCGC
GCCCGGCGAGGATCCCCGCGGCCTCGGCCTCACCGAGACCGGCTACCACTTCATCGGCGGCGTGCTGCGCCATGGCCGGG
CGCTCTGCGCCGCCTTCGCGCCCACCGTGAACAGCTACAAGCGCCTCGTGCGCCAGGGCGCGATGGCGGGCTTCTCCTGG
GCGCCGGTGTTCAACTCCTACGGCTCGAACAACCGCACCAATTCGGTGCGGGTGCCGGCCGGCGGTGGGCGCTGCGAGAG
CCGCAACGCGGACGGCGCGGTCAATCCCTACCTCGCCGCCGCCCTGGTGCTCGCGGCCGGGCTGGAGGGCGTGCGCGAGC
GGATCGACCCCGGCCAGCCCAACGAGGACAACCTCTACGCGCTGAGCGACGGCGAGCGCCGGGCGCGCGGCATCGCGTTC
CTGCCCCAGACCCTGCAGGAGGCGGTCGATGCCTTCGCCGCCGATCCCCTGGTCGAGGCGACGCTGGGCTCAGGGCTGCG
CGAAGAGTTCATCCGCACCAAGCGCGAGGAGTGGAACGCCTACCATCTCACGGTGAGCCCGTGGGAAATCGAGCGCTACA
GCCACCTGTTCTAG

Upstream 100 bases:

>100_bases
GTCGAGGACCTGACCGCCCTCGTCCGCCGCGGGCTCAACGAGACCCCGCCCCTTCCCCATTCCTACGACGGCTCCGCCGT
CCCCATGCCAGGAGCCTGAA

Downstream 100 bases:

>100_bases
GCGGAGGCTCCCATGAAGACCGTCATCGCCTTCGCGAATTCTTCCGCAGAGCCCTCGACCTACCGGCCGGCGCCCGAGCG
CATCCTGGCCGGAGACCCCG

Product: glutamine synthetaseII

Products: NA

Alternate protein names: Glutamate--ammonia ligase; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MGHANDIDPQVRAVQEDLRAKGVKYVIGAYVDIHGAQKAKVVPIDHLPQMAAGSERYTGYALDGLGQAPNEDELASVPDF
SQVIQLPWESKLAWAPADLTFQGKPYPLSTRVALKNVLAQAEAMGFGFNLGIECEIFLLRQAADGSLHTPVADDKLVKPC
YDVRGFIDNFSWLDKVATTINDLGWDLYSFDHEDANAQFEFDFQYADALTMCDRLTFFRMMTKHYAKEEGLIATMMPKPF
ADRTGNGAHFNMSLSDRETGRNLFACAPGEDPRGLGLTETGYHFIGGVLRHGRALCAAFAPTVNSYKRLVRQGAMAGFSW
APVFNSYGSNNRTNSVRVPAGGGRCESRNADGAVNPYLAAALVLAAGLEGVRERIDPGQPNEDNLYALSDGERRARGIAF
LPQTLQEAVDAFAADPLVEATLGSGLREEFIRTKREEWNAYHLTVSPWEIERYSHLF

Sequences:

>Translated_457_residues
MGHANDIDPQVRAVQEDLRAKGVKYVIGAYVDIHGAQKAKVVPIDHLPQMAAGSERYTGYALDGLGQAPNEDELASVPDF
SQVIQLPWESKLAWAPADLTFQGKPYPLSTRVALKNVLAQAEAMGFGFNLGIECEIFLLRQAADGSLHTPVADDKLVKPC
YDVRGFIDNFSWLDKVATTINDLGWDLYSFDHEDANAQFEFDFQYADALTMCDRLTFFRMMTKHYAKEEGLIATMMPKPF
ADRTGNGAHFNMSLSDRETGRNLFACAPGEDPRGLGLTETGYHFIGGVLRHGRALCAAFAPTVNSYKRLVRQGAMAGFSW
APVFNSYGSNNRTNSVRVPAGGGRCESRNADGAVNPYLAAALVLAAGLEGVRERIDPGQPNEDNLYALSDGERRARGIAF
LPQTLQEAVDAFAADPLVEATLGSGLREEFIRTKREEWNAYHLTVSPWEIERYSHLF
>Mature_456_residues
GHANDIDPQVRAVQEDLRAKGVKYVIGAYVDIHGAQKAKVVPIDHLPQMAAGSERYTGYALDGLGQAPNEDELASVPDFS
QVIQLPWESKLAWAPADLTFQGKPYPLSTRVALKNVLAQAEAMGFGFNLGIECEIFLLRQAADGSLHTPVADDKLVKPCY
DVRGFIDNFSWLDKVATTINDLGWDLYSFDHEDANAQFEFDFQYADALTMCDRLTFFRMMTKHYAKEEGLIATMMPKPFA
DRTGNGAHFNMSLSDRETGRNLFACAPGEDPRGLGLTETGYHFIGGVLRHGRALCAAFAPTVNSYKRLVRQGAMAGFSWA
PVFNSYGSNNRTNSVRVPAGGGRCESRNADGAVNPYLAAALVLAAGLEGVRERIDPGQPNEDNLYALSDGERRARGIAFL
PQTLQEAVDAFAADPLVEATLGSGLREEFIRTKREEWNAYHLTVSPWEIERYSHLF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=449, Percent_Identity=21.6035634743875, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1790301, Length=287, Percent_Identity=33.7979094076655, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87081870, Length=459, Percent_Identity=25.9259259259259, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50355; Mature: 50224

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHANDIDPQVRAVQEDLRAKGVKYVIGAYVDIHGAQKAKVVPIDHLPQMAAGSERYTGY
CCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEEHHHCCHHHCCCCCCCCE
ALDGLGQAPNEDELASVPDFSQVIQLPWESKLAWAPADLTFQGKPYPLSTRVALKNVLAQ
EECCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCEEECCCCCCCHHHHHHHHHHHH
AEAMGFGFNLGIECEIFLLRQAADGSLHTPVADDKLVKPCYDVRGFIDNFSWLDKVATTI
HHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NDLGWDLYSFDHEDANAQFEFDFQYADALTMCDRLTFFRMMTKHYAKEEGLIATMMPKPF
HHHCCHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
ADRTGNGAHFNMSLSDRETGRNLFACAPGEDPRGLGLTETGYHFIGGVLRHGRALCAAFA
CCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
PTVNSYKRLVRQGAMAGFSWAPVFNSYGSNNRTNSVRVPAGGGRCESRNADGAVNPYLAA
HHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHH
ALVLAAGLEGVRERIDPGQPNEDNLYALSDGERRARGIAFLPQTLQEAVDAFAADPLVEA
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHCCHHHHH
TLGSGLREEFIRTKREEWNAYHLTVSPWEIERYSHLF
HHCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCC
>Mature Secondary Structure 
GHANDIDPQVRAVQEDLRAKGVKYVIGAYVDIHGAQKAKVVPIDHLPQMAAGSERYTGY
CCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEEHHHCCHHHCCCCCCCCE
ALDGLGQAPNEDELASVPDFSQVIQLPWESKLAWAPADLTFQGKPYPLSTRVALKNVLAQ
EECCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCEEECCCCCCCHHHHHHHHHHHH
AEAMGFGFNLGIECEIFLLRQAADGSLHTPVADDKLVKPCYDVRGFIDNFSWLDKVATTI
HHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NDLGWDLYSFDHEDANAQFEFDFQYADALTMCDRLTFFRMMTKHYAKEEGLIATMMPKPF
HHHCCHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
ADRTGNGAHFNMSLSDRETGRNLFACAPGEDPRGLGLTETGYHFIGGVLRHGRALCAAFA
CCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
PTVNSYKRLVRQGAMAGFSWAPVFNSYGSNNRTNSVRVPAGGGRCESRNADGAVNPYLAA
HHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHH
ALVLAAGLEGVRERIDPGQPNEDNLYALSDGERRARGIAFLPQTLQEAVDAFAADPLVEA
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHCCHHHHH
TLGSGLREEFIRTKREEWNAYHLTVSPWEIERYSHLF
HHCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1356885 [H]