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The map label for this gene is 218531927

Identifier: 218531927

GI number: 218531927

Start: 4222789

End: 4224624

Strand: Reverse

Name: 218531927

Synonym: Mchl_4015

Alternate gene names: NA

Gene position: 4224624-4222789 (Counterclockwise)

Preceding gene: 218531928

Following gene: 218531926

Centisome position: 73.12

GC content: 74.07

Gene sequence:

>1836_bases
ATGCCGGTTCCCGCCTTCCCGAGCCTCAGCGCGCTCCACACCGCCTATGCCGAGGGGCTCTCGCCCGAGGCGGTTCTGGC
CGAGATCGACCGCCGGATCGCAGCCGCCGACGATCCCGGCATCTTCCTCGCACGGGTGCCGGCGGCGGAGATGCAGGCCG
CCGCCCGCGCGCTGGGCCCCTTCGACCCCGCCAACAAGCCGCTCTGGGGCGTGCCCTTCGCCGTGAAGGACAATGTCGAC
GTCGCAGGCCTGCCCACCACCGCCGCCTGCCCGGATTTCGCCTACACGCCGCAGGCGACCGCCCTGGCGGTCGAGCGCCT
GCTCGCGGCCGGCGCGATCCTCGTCGGCAAGACCAATCTCGATCAGTTCGCCACGGGGCTCGTCGGCGTGCGCACGCCCT
ATCCAGTGCCGAAGAACGCCATCGATCCGGCGATCGTGCCGGGCGGCTCCTCGTCCGGCTCGGCGGTTGCAGTGGCGCGC
GGCCTCGTCACCTTCGCGCTCGGCACCGACACGGCGGGTTCGGGCCGGGTGCCGGCCGGCCTCAACAACATCGTCGGGCT
CAAGCCCTCCCTCGGCAGCGTCTCGGGCCGCGGCGTGGTCCCGGCCTGCCGCACGCTGGACACCCTCTCCGTCTTCGCCG
GCACCGTGACGGAGGCCGATACGGTCTTTCGGATCATGGCCGGATACGATCCGCAGGATCCGTATTCGCGCGCCCTGCCC
GTTCCGCCCCGCCCCGGTGCCCTGCCGCCGGGCTTGCGCGTCGGCGTGCCGGATGCGGCGGGCCTGATCTTTGCCGGCGA
CGCGCTCTCGGCGGCGGCTTTCGACGGGGCACGCGCCGACCTGCACACGGTGACTGGTGCTGCAGCGACGGCGGTCGATC
TCGCGCCCTTCTTCGCGGTGGCGGGGCTTCTCTATGCCGGCCCCTGGGTGGCCGAGCGCTATCAGGCGATCCGCGGCTTC
ATCGAGGAGCGCCCGGAGGCCCTGCACCCGACGACGCGGGCGATCATCGGCGCGGCCACCGGTCACTCGGCGGCGGACGC
CTTCGCCGGCCTCTACCGCCTCGCCGAACTGCGCCGGGCGACGGAAGCCGTCTGGCGCGGAATCGACGTGCTCGTGGTGC
CGACCTATCCGCGTCCGCGCCGGGTCGCCGACCTCGCCGCCGACCCGGTCGGCCCCAACAGCGAGCTCGGCACCTACACC
AACTTCGTCAACCTGCTCGATCTCTGCGCCCTCGCGGTGCCGGGCCGCTTCCGCACCGACGGCTTCCCGTCCGGCGTCAC
CCTGATCGCGCCGCGCGGGGCCGACGGCCTCATCGCCGAACTCGGCGCCCGCCTCCACGCGGCGGCCGGCGGCACCCTCG
GAGCAAGCGGCGTGCCGATCCCGGCGGAGGCCGCATCGCCGGGGGATCGCGCGAAGGGGGGAGATCGCGCGCAAGGGGAC
GAGATCGAGATCGCGGTGGTCGGCGCGCACCTGTCCGGCCTGCCGCTCAACGGCGAGCTGACCGCCCGCGGCGCCCGCTT
CCTGCGCGCCGTCCCCACGACGCCGGATTACCGCCTGCACGCGCTGCCCGGCGGACCGCCGGCCCGGCCCGGCCTGATCC
GCGTCGCGCCCGGCTCGGGCCATGCGATCGAGACCGAAATCTGGGCGCTGGCGCCGGACGCTTTCGGCTCCTTCGTGGCG
GGAATTCCCGCGCCGCTTGCGATCGGCACGCTGTCGCTGGCCGACGGCACGGCGCCGAAGGGATTTTTGGCGGAAGCGGC
CGGGCTGACGGGCGCGCGCGACATCAGCGATCACGGCGGCTGGCGTGCCTATCTCGCCTCCGGGACCGCGACGTAG

Upstream 100 bases:

>100_bases
GGCCGCCGGGCCGACGGGCCGTCAGGCTCGGGCCGCCGGAGCCGTTCCCCTTGGAATTTCGGGCCGCGTGCCGCCGGCCC
TTCACGGACGTGAGAGATCG

Downstream 100 bases:

>100_bases
GGCCGTGCCCAGCCCGATCGCATCGGGCCGGCGTCGCCCGGTCATTGTTGCGACCAGCCTCCCCTGAGAGAAACCTCTCG
GGGGGATGAACCCTTTCCTT

Product: allophanate hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MPVPAFPSLSALHTAYAEGLSPEAVLAEIDRRIAAADDPGIFLARVPAAEMQAAARALGPFDPANKPLWGVPFAVKDNVD
VAGLPTTAACPDFAYTPQATALAVERLLAAGAILVGKTNLDQFATGLVGVRTPYPVPKNAIDPAIVPGGSSSGSAVAVAR
GLVTFALGTDTAGSGRVPAGLNNIVGLKPSLGSVSGRGVVPACRTLDTLSVFAGTVTEADTVFRIMAGYDPQDPYSRALP
VPPRPGALPPGLRVGVPDAAGLIFAGDALSAAAFDGARADLHTVTGAAATAVDLAPFFAVAGLLYAGPWVAERYQAIRGF
IEERPEALHPTTRAIIGAATGHSAADAFAGLYRLAELRRATEAVWRGIDVLVVPTYPRPRRVADLAADPVGPNSELGTYT
NFVNLLDLCALAVPGRFRTDGFPSGVTLIAPRGADGLIAELGARLHAAAGGTLGASGVPIPAEAASPGDRAKGGDRAQGD
EIEIAVVGAHLSGLPLNGELTARGARFLRAVPTTPDYRLHALPGGPPARPGLIRVAPGSGHAIETEIWALAPDAFGSFVA
GIPAPLAIGTLSLADGTAPKGFLAEAAGLTGARDISDHGGWRAYLASGTAT

Sequences:

>Translated_611_residues
MPVPAFPSLSALHTAYAEGLSPEAVLAEIDRRIAAADDPGIFLARVPAAEMQAAARALGPFDPANKPLWGVPFAVKDNVD
VAGLPTTAACPDFAYTPQATALAVERLLAAGAILVGKTNLDQFATGLVGVRTPYPVPKNAIDPAIVPGGSSSGSAVAVAR
GLVTFALGTDTAGSGRVPAGLNNIVGLKPSLGSVSGRGVVPACRTLDTLSVFAGTVTEADTVFRIMAGYDPQDPYSRALP
VPPRPGALPPGLRVGVPDAAGLIFAGDALSAAAFDGARADLHTVTGAAATAVDLAPFFAVAGLLYAGPWVAERYQAIRGF
IEERPEALHPTTRAIIGAATGHSAADAFAGLYRLAELRRATEAVWRGIDVLVVPTYPRPRRVADLAADPVGPNSELGTYT
NFVNLLDLCALAVPGRFRTDGFPSGVTLIAPRGADGLIAELGARLHAAAGGTLGASGVPIPAEAASPGDRAKGGDRAQGD
EIEIAVVGAHLSGLPLNGELTARGARFLRAVPTTPDYRLHALPGGPPARPGLIRVAPGSGHAIETEIWALAPDAFGSFVA
GIPAPLAIGTLSLADGTAPKGFLAEAAGLTGARDISDHGGWRAYLASGTAT
>Mature_610_residues
PVPAFPSLSALHTAYAEGLSPEAVLAEIDRRIAAADDPGIFLARVPAAEMQAAARALGPFDPANKPLWGVPFAVKDNVDV
AGLPTTAACPDFAYTPQATALAVERLLAAGAILVGKTNLDQFATGLVGVRTPYPVPKNAIDPAIVPGGSSSGSAVAVARG
LVTFALGTDTAGSGRVPAGLNNIVGLKPSLGSVSGRGVVPACRTLDTLSVFAGTVTEADTVFRIMAGYDPQDPYSRALPV
PPRPGALPPGLRVGVPDAAGLIFAGDALSAAAFDGARADLHTVTGAAATAVDLAPFFAVAGLLYAGPWVAERYQAIRGFI
EERPEALHPTTRAIIGAATGHSAADAFAGLYRLAELRRATEAVWRGIDVLVVPTYPRPRRVADLAADPVGPNSELGTYTN
FVNLLDLCALAVPGRFRTDGFPSGVTLIAPRGADGLIAELGARLHAAAGGTLGASGVPIPAEAASPGDRAKGGDRAQGDE
IEIAVVGAHLSGLPLNGELTARGARFLRAVPTTPDYRLHALPGGPPARPGLIRVAPGSGHAIETEIWALAPDAFGSFVAG
IPAPLAIGTLSLADGTAPKGFLAEAAGLTGARDISDHGGWRAYLASGTAT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=427, Percent_Identity=28.1030444964871, Blast_Score=95, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=448, Percent_Identity=29.0178571428571, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=186, Percent_Identity=32.258064516129, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=172, Percent_Identity=34.8837209302326, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=552, Percent_Identity=45.2898550724638, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323950, Length=191, Percent_Identity=37.17277486911, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648113, Length=355, Percent_Identity=32.112676056338, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 61890; Mature: 61759

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPAFPSLSALHTAYAEGLSPEAVLAEIDRRIAAADDPGIFLARVPAAEMQAAARALGP
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCC
FDPANKPLWGVPFAVKDNVDVAGLPTTAACPDFAYTPQATALAVERLLAAGAILVGKTNL
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCH
DQFATGLVGVRTPYPVPKNAIDPAIVPGGSSSGSAVAVARGLVTFALGTDTAGSGRVPAG
HHHHHHHEECCCCCCCCCCCCCCCEECCCCCCCCHHHHHHCEEEEEECCCCCCCCCCCCC
LNNIVGLKPSLGSVSGRGVVPACRTLDTLSVFAGTVTEADTVFRIMAGYDPQDPYSRALP
CCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHCCCC
VPPRPGALPPGLRVGVPDAAGLIFAGDALSAAAFDGARADLHTVTGAAATAVDLAPFFAV
CCCCCCCCCCCCEECCCCCCCEEEECCCHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHH
AGLLYAGPWVAERYQAIRGFIEERPEALHPTTRAIIGAATGHSAADAFAGLYRLAELRRA
HHHHHCCHHHHHHHHHHHHHHHHCCHHCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHH
TEAVWRGIDVLVVPTYPRPRRVADLAADPVGPNSELGTYTNFVNLLDLCALAVPGRFRTD
HHHHHCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
GFPSGVTLIAPRGADGLIAELGARLHAAAGGTLGASGVPIPAEAASPGDRAKGGDRAQGD
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EIEIAVVGAHLSGLPLNGELTARGARFLRAVPTTPDYRLHALPGGPPARPGLIRVAPGSG
EEEEEEEECCCCCCCCCCCCHHCHHHHHEECCCCCCCEEEECCCCCCCCCCEEEECCCCC
HAIETEIWALAPDAFGSFVAGIPAPLAIGTLSLADGTAPKGFLAEAAGLTGARDISDHGG
CEEEEEEEEECCHHHHHHHHCCCCCHHEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCC
WRAYLASGTAT
EEEEEECCCCC
>Mature Secondary Structure 
PVPAFPSLSALHTAYAEGLSPEAVLAEIDRRIAAADDPGIFLARVPAAEMQAAARALGP
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCC
FDPANKPLWGVPFAVKDNVDVAGLPTTAACPDFAYTPQATALAVERLLAAGAILVGKTNL
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCH
DQFATGLVGVRTPYPVPKNAIDPAIVPGGSSSGSAVAVARGLVTFALGTDTAGSGRVPAG
HHHHHHHEECCCCCCCCCCCCCCCEECCCCCCCCHHHHHHCEEEEEECCCCCCCCCCCCC
LNNIVGLKPSLGSVSGRGVVPACRTLDTLSVFAGTVTEADTVFRIMAGYDPQDPYSRALP
CCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHCCCC
VPPRPGALPPGLRVGVPDAAGLIFAGDALSAAAFDGARADLHTVTGAAATAVDLAPFFAV
CCCCCCCCCCCCEECCCCCCCEEEECCCHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHH
AGLLYAGPWVAERYQAIRGFIEERPEALHPTTRAIIGAATGHSAADAFAGLYRLAELRRA
HHHHHCCHHHHHHHHHHHHHHHHCCHHCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHH
TEAVWRGIDVLVVPTYPRPRRVADLAADPVGPNSELGTYTNFVNLLDLCALAVPGRFRTD
HHHHHCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
GFPSGVTLIAPRGADGLIAELGARLHAAAGGTLGASGVPIPAEAASPGDRAKGGDRAQGD
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EIEIAVVGAHLSGLPLNGELTARGARFLRAVPTTPDYRLHALPGGPPARPGLIRVAPGSG
EEEEEEEECCCCCCCCCCCCHHCHHHHHEECCCCCCCEEEECCCCCCCCCCEEEECCCCC
HAIETEIWALAPDAFGSFVAGIPAPLAIGTLSLADGTAPKGFLAEAAGLTGARDISDHGG
CEEEEEEEEECCHHHHHHHHCCCCCHHEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCC
WRAYLASGTAT
EEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9809753 [H]