Click here to switch to the map view.

The map label for this gene is lptD [H]

Identifier: 218531864

GI number: 218531864

Start: 4135297

End: 4137927

Strand: Reverse

Name: lptD [H]

Synonym: Mchl_3941

Alternate gene names: 218531864

Gene position: 4137927-4135297 (Counterclockwise)

Preceding gene: 218531865

Following gene: 218531863

Centisome position: 71.62

GC content: 67.05

Gene sequence:

>2631_bases
GTGATCGGCGTGCAGGGGGCTGCACAGGGTTCGAGGGACGCGTTGCGTAAGGTCGCCGACATCGCGATCACGGTCTCCAT
GGCCGCACTGCTGACGGCAGCCTACGGGCTCGCCGCGCCGCGCGCCCATGCCCAGGCCGGACTGGAGAAGCTCGCCGGCT
CCAAGCCCGGCGAGAAGGGCCAGCGCCTGCTCGTCGAGGCCGACGAGCTCATCTACGACAACGACCGCAACACCGTGACC
GCGCGCGGCAATGCCGAGCTGCATTACGGCGCGCGCACGCTCCAGGCCGACCGGGTGCGCTACGACCGCGGCACGAGCCG
CGTCTTCGCCGAGGGCAATGTCCGCCTCACCGACGAGACCGGAGCGCTGGTCACCGGCGAGCGGATGGAGCTGACCGACG
ACTTCAAGAACGGCTTCGTCGAGGCGCTGCGCATCCAGCAGACCGTGGTGATGAAGGGCGAGACGGTGCGCACCCGCATC
TCCGCCCCGCGGGCGGAGCGAATCGCCGGCGAGCAATCGAGCTTCGACTACGCGACCTACACCGCCTGCGAGCCGTGCAA
GGACCATCCGGAGACGCCGCCGCTGTGGCAGATCAAGGCGGCCAAGATCATCCACAACAATGAGACCCACACGATCAGTT
ACGAGGACTCGAGCCTCGAAATCGGTGGGATCCCGGTCGCCTACCTGCCCTATTTCGAGTCCGCCGACCCGACGGTGAAG
CGCAAGACCGGCTTCCTGACGCCGCGCTTCATCACCTCGACCCCGCTCGGCAGCGGCGTCGCTACCCCGTATTTCATCAA
CCTCGATCCGAGCTACGACCTGACGATCACCCCGGCCTTCGTCTCGCGCCAGGGTGTGCTGGGCCAGGCCGAGTTCCGCC
AGCGCCTCGACAACGGCAGCTACAATCTGCGCCTGTCGGGCATCTTCCAATCGACGCCCTCGGCCTTCCTGCCGGGGCCG
CTGGGTGCGGCTGATCGCGAGTTCCGCGGCTCGGTCGAGACGCGCGGGCGCTTCTTCATCAACGAGCACTGGCGCACGGG
TTGGGACCTCGTCGGCGTGACCGACAAGTGGTTCCTCGACAATTACCGCATCCGCAACCAGAGCATCACCACCGACTATT
TCCGGGAGGCGGTGTCGAGCGCCTACCTGATCGGCCAGGGCGACCGCTCGTGGTTCGAGGCGCGCGGCTACTACTTCAAG
GCCCTGTCGAGCTTCGACTGGCAGAAGGAGCAGCCCGTCGTCCTGCCGGTGATCGATTACGACAAGCGCATCAATGGGCC
GGGCAAGCTCGGCGGCGAGGTGCGGTTCGAGGCCAACGTCACGAGCCTCAGCCGCGAGGCCACCGACTTCCAGGGCATCC
CGCGCACGGGCAGCTACCTGTTCGCCCCGAGCGTCAACGGGATCAGCTATCCGCTCTACGAGACCTGCACCACCTTCGTG
CGCGACCGCTGCATCGTGCGCGGTTTGGGCGGCGTCGACACGCGGCTCTCGGCCCAGGTCTCCTGGCGCCGCAGCTTCAT
CGACGATTTCGGCCAGGTCTTCACGCCGTTCACCTATCTGCGCACCGACGCCTTCTTCGTGAACCCGAGCCGCTCGGGCT
TCCAGAACGCGCTGGTGAACAACATCACCACCGTCGATGACGGCTTCTCCGGCCGGGTCATGCCGGCGATCGGACTTGAC
TACCGCTATCCCTTCGTCGCCGATTTCGGCGGGCTCGGTGTCCACACCTTGGAGCCGATCGCCCAGGTGATTGCCCGGCC
CTCCGAGACCCGGATCGGCCGCCTGCCCAACGAGGACGCACAGAGCCTCGTCTTCGACGACACCTCGCTGTTCGAGTGGG
ACAAGTTCTCCGGCTACGACCGGGTCGAGGGCGGCGTGCGGGCCAATCTCGGCGCGCAATATTCGGTCGTGACCCCGTCC
GGCTGGTACGCGAACCTGATGTTCGGCGAGTCGATCCAGATCGCGGGCGTGAACTCGTTCCGCCGTGGGGATCTGGCCAA
TGTCGGCCTCGATTCCGGCCTGGAGAACCGCCGCTCGGATTTCGTCGGCCGCTTCCAGCTCTCGCCCAACCAGAACATCA
GCTTCATCAGCCGCGCCCGCTTCGACCAGCGCGACTTCGCCGTGAACCGGTTCGAGACCGGCGTGACCGCGCGGTTCTCG
CCGTTCCTGCCGCTCGAAACCTCGCTGTTCTACTCGTTCTACGAGGCGCAGCCGGCGCTCGGATTCGCCCATCGCCGCGA
GGGCGTGACGGCCTCGGCCACCTACCGCATCACCCCGAACTGGTTCGTCACCGGCTCGGCGCTCGTCGACTTCACCCGCT
ACCTCGACACCCGCGACACCTTCGCCGACCTCTATACCGCCTATCTCACCAACCCGGTCGGCCTCGCCCCGGTCTACAGC
AGCCCCGGCCTGACCTACCTCTCCGGCCTGAGCCTCGGCGGCGGCTACCAGGACGAGTGCACCACGGTCTCGCTGAACTA
CATCGTCTCGCCCATCGCCACGGCGATCGGCGTGCGCGAGCGAAACCAGACCGTGCTGCTGCGCCTGGAGCTGAAGACTT
TGGGCGAGGCGGACCTGCGCCAGAATGTCAGCAGCTCGACCACCGCCGACGGTATCGCCTCGGTGCGCTGA

Upstream 100 bases:

>100_bases
GGTAGGAAGCTTGCTCGGTACGCTCACGCTTCTCTACCAGGAGGACGGCTGATGCACGGTTGTCCGGATCGGGACGGGTT
CGGTCGTGCAAGGGACTATC

Downstream 100 bases:

>100_bases
GATGGCCGTCGGCGCCGGGATACCACGATGCTGAAGGGGGCGGCCGATTCGCTGATCCGCGCCTACGGCGCCCTGGCCAC
GCTCCCGTTCAGGTTCCGCC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 876; Mature: 876

Protein sequence:

>876_residues
MIGVQGAAQGSRDALRKVADIAITVSMAALLTAAYGLAAPRAHAQAGLEKLAGSKPGEKGQRLLVEADELIYDNDRNTVT
ARGNAELHYGARTLQADRVRYDRGTSRVFAEGNVRLTDETGALVTGERMELTDDFKNGFVEALRIQQTVVMKGETVRTRI
SAPRAERIAGEQSSFDYATYTACEPCKDHPETPPLWQIKAAKIIHNNETHTISYEDSSLEIGGIPVAYLPYFESADPTVK
RKTGFLTPRFITSTPLGSGVATPYFINLDPSYDLTITPAFVSRQGVLGQAEFRQRLDNGSYNLRLSGIFQSTPSAFLPGP
LGAADREFRGSVETRGRFFINEHWRTGWDLVGVTDKWFLDNYRIRNQSITTDYFREAVSSAYLIGQGDRSWFEARGYYFK
ALSSFDWQKEQPVVLPVIDYDKRINGPGKLGGEVRFEANVTSLSREATDFQGIPRTGSYLFAPSVNGISYPLYETCTTFV
RDRCIVRGLGGVDTRLSAQVSWRRSFIDDFGQVFTPFTYLRTDAFFVNPSRSGFQNALVNNITTVDDGFSGRVMPAIGLD
YRYPFVADFGGLGVHTLEPIAQVIARPSETRIGRLPNEDAQSLVFDDTSLFEWDKFSGYDRVEGGVRANLGAQYSVVTPS
GWYANLMFGESIQIAGVNSFRRGDLANVGLDSGLENRRSDFVGRFQLSPNQNISFISRARFDQRDFAVNRFETGVTARFS
PFLPLETSLFYSFYEAQPALGFAHRREGVTASATYRITPNWFVTGSALVDFTRYLDTRDTFADLYTAYLTNPVGLAPVYS
SPGLTYLSGLSLGGGYQDECTTVSLNYIVSPIATAIGVRERNQTVLLRLELKTLGEADLRQNVSSSTTADGIASVR

Sequences:

>Translated_876_residues
MIGVQGAAQGSRDALRKVADIAITVSMAALLTAAYGLAAPRAHAQAGLEKLAGSKPGEKGQRLLVEADELIYDNDRNTVT
ARGNAELHYGARTLQADRVRYDRGTSRVFAEGNVRLTDETGALVTGERMELTDDFKNGFVEALRIQQTVVMKGETVRTRI
SAPRAERIAGEQSSFDYATYTACEPCKDHPETPPLWQIKAAKIIHNNETHTISYEDSSLEIGGIPVAYLPYFESADPTVK
RKTGFLTPRFITSTPLGSGVATPYFINLDPSYDLTITPAFVSRQGVLGQAEFRQRLDNGSYNLRLSGIFQSTPSAFLPGP
LGAADREFRGSVETRGRFFINEHWRTGWDLVGVTDKWFLDNYRIRNQSITTDYFREAVSSAYLIGQGDRSWFEARGYYFK
ALSSFDWQKEQPVVLPVIDYDKRINGPGKLGGEVRFEANVTSLSREATDFQGIPRTGSYLFAPSVNGISYPLYETCTTFV
RDRCIVRGLGGVDTRLSAQVSWRRSFIDDFGQVFTPFTYLRTDAFFVNPSRSGFQNALVNNITTVDDGFSGRVMPAIGLD
YRYPFVADFGGLGVHTLEPIAQVIARPSETRIGRLPNEDAQSLVFDDTSLFEWDKFSGYDRVEGGVRANLGAQYSVVTPS
GWYANLMFGESIQIAGVNSFRRGDLANVGLDSGLENRRSDFVGRFQLSPNQNISFISRARFDQRDFAVNRFETGVTARFS
PFLPLETSLFYSFYEAQPALGFAHRREGVTASATYRITPNWFVTGSALVDFTRYLDTRDTFADLYTAYLTNPVGLAPVYS
SPGLTYLSGLSLGGGYQDECTTVSLNYIVSPIATAIGVRERNQTVLLRLELKTLGEADLRQNVSSSTTADGIASVR
>Mature_876_residues
MIGVQGAAQGSRDALRKVADIAITVSMAALLTAAYGLAAPRAHAQAGLEKLAGSKPGEKGQRLLVEADELIYDNDRNTVT
ARGNAELHYGARTLQADRVRYDRGTSRVFAEGNVRLTDETGALVTGERMELTDDFKNGFVEALRIQQTVVMKGETVRTRI
SAPRAERIAGEQSSFDYATYTACEPCKDHPETPPLWQIKAAKIIHNNETHTISYEDSSLEIGGIPVAYLPYFESADPTVK
RKTGFLTPRFITSTPLGSGVATPYFINLDPSYDLTITPAFVSRQGVLGQAEFRQRLDNGSYNLRLSGIFQSTPSAFLPGP
LGAADREFRGSVETRGRFFINEHWRTGWDLVGVTDKWFLDNYRIRNQSITTDYFREAVSSAYLIGQGDRSWFEARGYYFK
ALSSFDWQKEQPVVLPVIDYDKRINGPGKLGGEVRFEANVTSLSREATDFQGIPRTGSYLFAPSVNGISYPLYETCTTFV
RDRCIVRGLGGVDTRLSAQVSWRRSFIDDFGQVFTPFTYLRTDAFFVNPSRSGFQNALVNNITTVDDGFSGRVMPAIGLD
YRYPFVADFGGLGVHTLEPIAQVIARPSETRIGRLPNEDAQSLVFDDTSLFEWDKFSGYDRVEGGVRANLGAQYSVVTPS
GWYANLMFGESIQIAGVNSFRRGDLANVGLDSGLENRRSDFVGRFQLSPNQNISFISRARFDQRDFAVNRFETGVTARFS
PFLPLETSLFYSFYEAQPALGFAHRREGVTASATYRITPNWFVTGSALVDFTRYLDTRDTFADLYTAYLTNPVGLAPVYS
SPGLTYLSGLSLGGGYQDECTTVSLNYIVSPIATAIGVRERNQTVLLRLELKTLGEADLRQNVSSSTTADGIASVR

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=639, Percent_Identity=22.2222222222222, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 96963; Mature: 96963

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00879 ODR_DC_2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGVQGAAQGSRDALRKVADIAITVSMAALLTAAYGLAAPRAHAQAGLEKLAGSKPGEKG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
QRLLVEADELIYDNDRNTVTARGNAELHYGARTLQADRVRYDRGTSRVFAEGNVRLTDET
CEEEEEECCEEEECCCCEEEECCCCEEEECCHHHHHHHEEECCCCCEEEECCCEEEECCC
GALVTGERMELTDDFKNGFVEALRIQQTVVMKGETVRTRISAPRAERIAGEQSSFDYATY
CCEEECCCEECHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCHHHHHCCCCCCCCEEEE
TACEPCKDHPETPPLWQIKAAKIIHNNETHTISYEDSSLEIGGIPVAYLPYFESADPTVK
CCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCEEECCCCEEEECCCCCCCCCHH
RKTGFLTPRFITSTPLGSGVATPYFINLDPSYDLTITPAFVSRQGVLGQAEFRQRLDNGS
HHCCCCCCCEEECCCCCCCCCCCEEEECCCCCCEEEEHHHHCCCCCCCHHHHHHHHCCCC
YNLRLSGIFQSTPSAFLPGPLGAADREFRGSVETRGRFFINEHWRTGWDLVGVTDKWFLD
EEEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEECCCCCCCEEEECCHHEECC
NYRIRNQSITTDYFREAVSSAYLIGQGDRSWFEARGYYFKALSSFDWQKEQPVVLPVIDY
CEEECCCCCCHHHHHHHHHCEEEEECCCCHHHHHCCHHEEHHCCCCCCCCCCEEEEEECC
DKRINGPGKLGGEVRFEANVTSLSREATDFQGIPRTGSYLFAPSVNGISYPLYETCTTFV
CCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHH
RDRCIVRGLGGVDTRLSAQVSWRRSFIDDFGQVFTPFTYLRTDAFFVNPSRSGFQNALVN
HHHHHEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHEECCEEEECCCCCHHHHHHHH
NITTVDDGFSGRVMPAIGLDYRYPFVADFGGLGVHTLEPIAQVIARPSETRIGRLPNEDA
CCEECCCCCCCEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCC
QSLVFDDTSLFEWDKFSGYDRVEGGVRANLGAQYSVVTPSGWYANLMFGESIQIAGVNSF
CEEEECCCCCCCCCCCCCCCHHCCCCEECCCCEEEEECCCCCEEEEEECCEEEEEECCCC
RRGDLANVGLDSGLENRRSDFVGRFQLSPNQNISFISRARFDQRDFAVNRFETGVTARFS
CCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHCCCCEEECC
PFLPLETSLFYSFYEAQPALGFAHRREGVTASATYRITPNWFVTGSALVDFTRYLDTRDT
CCCCCHHHHHHHHHHCCCCCCHHHHHCCCEEEEEEEECCCEEEECHHHHHHHHHHCCHHH
FADLYTAYLTNPVGLAPVYSSPGLTYLSGLSLGGGYQDECTTVSLNYIVSPIATAIGVRE
HHHHHHHHHCCCCCCCEEECCCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCC
RNQTVLLRLELKTLGEADLRQNVSSSTTADGIASVR
CCCEEEEEEEHHHCCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIGVQGAAQGSRDALRKVADIAITVSMAALLTAAYGLAAPRAHAQAGLEKLAGSKPGEKG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
QRLLVEADELIYDNDRNTVTARGNAELHYGARTLQADRVRYDRGTSRVFAEGNVRLTDET
CEEEEEECCEEEECCCCEEEECCCCEEEECCHHHHHHHEEECCCCCEEEECCCEEEECCC
GALVTGERMELTDDFKNGFVEALRIQQTVVMKGETVRTRISAPRAERIAGEQSSFDYATY
CCEEECCCEECHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCHHHHHCCCCCCCCEEEE
TACEPCKDHPETPPLWQIKAAKIIHNNETHTISYEDSSLEIGGIPVAYLPYFESADPTVK
CCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCEEECCCCEEEECCCCCCCCCHH
RKTGFLTPRFITSTPLGSGVATPYFINLDPSYDLTITPAFVSRQGVLGQAEFRQRLDNGS
HHCCCCCCCEEECCCCCCCCCCCEEEECCCCCCEEEEHHHHCCCCCCCHHHHHHHHCCCC
YNLRLSGIFQSTPSAFLPGPLGAADREFRGSVETRGRFFINEHWRTGWDLVGVTDKWFLD
EEEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEECCCCCCCEEEECCHHEECC
NYRIRNQSITTDYFREAVSSAYLIGQGDRSWFEARGYYFKALSSFDWQKEQPVVLPVIDY
CEEECCCCCCHHHHHHHHHCEEEEECCCCHHHHHCCHHEEHHCCCCCCCCCCEEEEEECC
DKRINGPGKLGGEVRFEANVTSLSREATDFQGIPRTGSYLFAPSVNGISYPLYETCTTFV
CCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHH
RDRCIVRGLGGVDTRLSAQVSWRRSFIDDFGQVFTPFTYLRTDAFFVNPSRSGFQNALVN
HHHHHEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHEECCEEEECCCCCHHHHHHHH
NITTVDDGFSGRVMPAIGLDYRYPFVADFGGLGVHTLEPIAQVIARPSETRIGRLPNEDA
CCEECCCCCCCEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCC
QSLVFDDTSLFEWDKFSGYDRVEGGVRANLGAQYSVVTPSGWYANLMFGESIQIAGVNSF
CEEEECCCCCCCCCCCCCCCHHCCCCEECCCCEEEEECCCCCEEEEEECCEEEEEECCCC
RRGDLANVGLDSGLENRRSDFVGRFQLSPNQNISFISRARFDQRDFAVNRFETGVTARFS
CCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHCCCCEEECC
PFLPLETSLFYSFYEAQPALGFAHRREGVTASATYRITPNWFVTGSALVDFTRYLDTRDT
CCCCCHHHHHHHHHHCCCCCCHHHHHCCCEEEEEEEECCCEEEECHHHHHHHHHHCCHHH
FADLYTAYLTNPVGLAPVYSSPGLTYLSGLSLGGGYQDECTTVSLNYIVSPIATAIGVRE
HHHHHHHHHCCCCCCCEEECCCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCC
RNQTVLLRLELKTLGEADLRQNVSSSTTADGIASVR
CCCEEEEEEEHHHCCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]