Click here to switch to the map view.

The map label for this gene is hsrA [H]

Identifier: 218531669

GI number: 218531669

Start: 3947262

End: 3948743

Strand: Reverse

Name: hsrA [H]

Synonym: Mchl_3738

Alternate gene names: 218531669

Gene position: 3948743-3947262 (Counterclockwise)

Preceding gene: 218531671

Following gene: 218531667

Centisome position: 68.34

GC content: 69.97

Gene sequence:

>1482_bases
ATGCGTCCCGCCCAGATCGTCCCCCTCGTCGTCGCCACCGCTTTGTTCATGGAGAACACCGACTCGACGGTGATCGCCAC
GGCCCTGCCGGCGATCGCGGCGAGCCTCGGTGTCGATCCGATCGCCCTCAAGCTCGCACTCACCGCCTATCTCGTCAGTC
TGGCGATCTTCATCCCGGTCTCGGGCTGGGCCGCCGACCGCTACGGCGCCCGCAACGTCTTCCGCGCCGCCCTGTGCGTA
TTCATGGCGGGCTCGCTCGCCTGCGCCGCCGCGAATTCGCTCACCGGTTTCGTCGCCGCCCGCTTCGTGCAGGGAATCGG
CGGGGCGATGATGGTGCCGGTGGGGCGCCTCGTGATCCTGCGCTCGGTGCCGAAATCCGAACTCGTTGGGGCGCTGGCCT
ACCTCACCATCCCCGCGCTGATCGGCCCGATCCTCGGGCCGCCGCTCGGCGGCTTCATCACCACCTATGCCGACTGGCGT
TGGATCTTCTTCATCAACATCCCGATCGGGCTCGCCGGCATCGTCCTCGCGACCCTGTATTTCGGCGACATCCGCGAGGA
AACGCGCCCGCCGCTCGACGTGACGGGCTTCGTCCTGTCAGGCGGCGGGCTCGCCCTGCTGATGCTCGGCTTTGCCGCGA
CCGGGCGCCATCTCCTGCCGGACGGTGTGTCCTGGGCCTGCATGGCCGGCGGGATCGTGCTCGTCGGGCTCTACCTGCGC
CACGCCAAGCGCACCGAGCATCCGCTCATCCGCCTCGACCTGCTCAAGCACGACACCTTCCGCGCGGCGGTGACGGGGGG
AAGCCTGTTCCGCATCGGTACGGGCGCCATCCCCTTCCTGCTGCCGCTGATGCTGCAGATCGGGTTCGGGCTCGATCCGC
TGCATTCAGGGCTCATCACCTTCGCCGCAGCCGCCGGCGCCCTTCTCATCAAGATCGTCGGCCCGCGCATCCTGCGCGCC
TACGGGTTCCGCCGGGTGATGGTGGTCAACGCGCTGGTCGCCTCCGCCTTTCTCGCCGCCAACGGGCTGTTCACGGCGCA
GACCCCGCACTGGATCATCGTCGGCGTGCTCCTGCTCGGCGGCTGCGTGCGCTCGCTGCAATTCACCTGCGTCAACGCCA
TCGCCTATGCCGACCTCGAATCGCGGGAGATGAGCGCGGCCACGAGTCTGGCAAGCGTCGCGCAGCAACTGTCCCTCAGC
CTCGGCGTCTCCATCGGCGCCCTGGCGCTGGAGGGCGCCGCCGCCTGGCACGGCCATGCCGGGATCGAGGCGGGAGATTT
CTCCCTCGCCTTCCTCGGGGTGGCCGTGGTCTCGGTGGGCTCGTTCTTCGTGTTCCGGCGGCTGGCGCCGGATGCCGGCG
CGGAGGTGTCGGGTCAGCGACGGGTCGCCGAGGCCGTGCCGAGACCCGCCGATGGTTCGCCCCAGAGCCTTGCCCCTGTT
ACGCGGCAGCCCGCGCCGGAGCCCGGCCGCCCGCCCGCTTGA

Upstream 100 bases:

>100_bases
TCGTCTTCGTGCAACGTCGCCGCGCCTCTCGCGCGCGACTTGCGCGCTCCTTGCGCGCCCCTTGCGCAACCTTCGGCGCG
TCGCGCGCTTCTCCCTGCCG

Downstream 100 bases:

>100_bases
CGCTGCCGGACTTAGGCCGCGCGGGCGCCTTGGCCGCCGGCTTGGCGGGGGCTCGTGCGGGCGCCGAAGCCTCGCCGGAG
CTGAAGACCCCGCGCCAGCT

Product: major facilitator superfamily MFS_1

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRPAQIVPLVVATALFMENTDSTVIATALPAIAASLGVDPIALKLALTAYLVSLAIFIPVSGWAADRYGARNVFRAALCV
FMAGSLACAAANSLTGFVAARFVQGIGGAMMVPVGRLVILRSVPKSELVGALAYLTIPALIGPILGPPLGGFITTYADWR
WIFFINIPIGLAGIVLATLYFGDIREETRPPLDVTGFVLSGGGLALLMLGFAATGRHLLPDGVSWACMAGGIVLVGLYLR
HAKRTEHPLIRLDLLKHDTFRAAVTGGSLFRIGTGAIPFLLPLMLQIGFGLDPLHSGLITFAAAAGALLIKIVGPRILRA
YGFRRVMVVNALVASAFLAANGLFTAQTPHWIIVGVLLLGGCVRSLQFTCVNAIAYADLESREMSAATSLASVAQQLSLS
LGVSIGALALEGAAAWHGHAGIEAGDFSLAFLGVAVVSVGSFFVFRRLAPDAGAEVSGQRRVAEAVPRPADGSPQSLAPV
TRQPAPEPGRPPA

Sequences:

>Translated_493_residues
MRPAQIVPLVVATALFMENTDSTVIATALPAIAASLGVDPIALKLALTAYLVSLAIFIPVSGWAADRYGARNVFRAALCV
FMAGSLACAAANSLTGFVAARFVQGIGGAMMVPVGRLVILRSVPKSELVGALAYLTIPALIGPILGPPLGGFITTYADWR
WIFFINIPIGLAGIVLATLYFGDIREETRPPLDVTGFVLSGGGLALLMLGFAATGRHLLPDGVSWACMAGGIVLVGLYLR
HAKRTEHPLIRLDLLKHDTFRAAVTGGSLFRIGTGAIPFLLPLMLQIGFGLDPLHSGLITFAAAAGALLIKIVGPRILRA
YGFRRVMVVNALVASAFLAANGLFTAQTPHWIIVGVLLLGGCVRSLQFTCVNAIAYADLESREMSAATSLASVAQQLSLS
LGVSIGALALEGAAAWHGHAGIEAGDFSLAFLGVAVVSVGSFFVFRRLAPDAGAEVSGQRRVAEAVPRPADGSPQSLAPV
TRQPAPEPGRPPA
>Mature_493_residues
MRPAQIVPLVVATALFMENTDSTVIATALPAIAASLGVDPIALKLALTAYLVSLAIFIPVSGWAADRYGARNVFRAALCV
FMAGSLACAAANSLTGFVAARFVQGIGGAMMVPVGRLVILRSVPKSELVGALAYLTIPALIGPILGPPLGGFITTYADWR
WIFFINIPIGLAGIVLATLYFGDIREETRPPLDVTGFVLSGGGLALLMLGFAATGRHLLPDGVSWACMAGGIVLVGLYLR
HAKRTEHPLIRLDLLKHDTFRAAVTGGSLFRIGTGAIPFLLPLMLQIGFGLDPLHSGLITFAAAAGALLIKIVGPRILRA
YGFRRVMVVNALVASAFLAANGLFTAQTPHWIIVGVLLLGGCVRSLQFTCVNAIAYADLESREMSAATSLASVAQQLSLS
LGVSIGALALEGAAAWHGHAGIEAGDFSLAFLGVAVVSVGSFFVFRRLAPDAGAEVSGQRRVAEAVPRPADGSPQSLAPV
TRQPAPEPGRPPA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=470, Percent_Identity=38.936170212766, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1788392, Length=461, Percent_Identity=36.4425162689805, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1789042, Length=440, Percent_Identity=27.9545454545455, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788710, Length=435, Percent_Identity=28.0459770114943, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87081983, Length=295, Percent_Identity=25.4237288135593, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=30.2469135802469, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=207, Percent_Identity=28.5024154589372, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=235, Percent_Identity=24.6808510638298, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51253; Mature: 51253

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAQIVPLVVATALFMENTDSTVIATALPAIAASLGVDPIALKLALTAYLVSLAIFIPV
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
SGWAADRYGARNVFRAALCVFMAGSLACAAANSLTGFVAARFVQGIGGAMMVPVGRLVIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RSVPKSELVGALAYLTIPALIGPILGPPLGGFITTYADWRWIFFINIPIGLAGIVLATLY
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEEEECCHHHHHHHHHHHH
FGDIREETRPPLDVTGFVLSGGGLALLMLGFAATGRHLLPDGVSWACMAGGIVLVGLYLR
HHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
HAKRTEHPLIRLDLLKHDTFRAAVTGGSLFRIGTGAIPFLLPLMLQIGFGLDPLHSGLIT
HHHCCCCCEEEEEEHHCCCCEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHH
FAAAAGALLIKIVGPRILRAYGFRRVMVVNALVASAFLAANGLFTAQTPHWIIVGVLLLG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHH
GCVRSLQFTCVNAIAYADLESREMSAATSLASVAQQLSLSLGVSIGALALEGAAAWHGHA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCCCC
GIEAGDFSLAFLGVAVVSVGSFFVFRRLAPDAGAEVSGQRRVAEAVPRPADGSPQSLAPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
TRQPAPEPGRPPA
CCCCCCCCCCCCC
>Mature Secondary Structure
MRPAQIVPLVVATALFMENTDSTVIATALPAIAASLGVDPIALKLALTAYLVSLAIFIPV
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
SGWAADRYGARNVFRAALCVFMAGSLACAAANSLTGFVAARFVQGIGGAMMVPVGRLVIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RSVPKSELVGALAYLTIPALIGPILGPPLGGFITTYADWRWIFFINIPIGLAGIVLATLY
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEEEECCHHHHHHHHHHHH
FGDIREETRPPLDVTGFVLSGGGLALLMLGFAATGRHLLPDGVSWACMAGGIVLVGLYLR
HHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
HAKRTEHPLIRLDLLKHDTFRAAVTGGSLFRIGTGAIPFLLPLMLQIGFGLDPLHSGLIT
HHHCCCCCEEEEEEHHCCCCEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHH
FAAAAGALLIKIVGPRILRAYGFRRVMVVNALVASAFLAANGLFTAQTPHWIIVGVLLLG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHH
GCVRSLQFTCVNAIAYADLESREMSAATSLASVAQQLSLSLGVSIGALALEGAAAWHGHA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCCCC
GIEAGDFSLAFLGVAVVSVGSFFVFRRLAPDAGAEVSGQRRVAEAVPRPADGSPQSLAPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
TRQPAPEPGRPPA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]